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The map label for this gene is haeIIIM [H]

Identifier: 169823586

GI number: 169823586

Start: 57683

End: 58675

Strand: Reverse

Name: haeIIIM [H]

Synonym: FMG_P0072

Alternate gene names: 169823586

Gene position: 58675-57683 (Counterclockwise)

Preceding gene: 169823587

Following gene: 169823585

Centisome position: 31.02

GC content: 32.43

Gene sequence:

>993_bases
ATGGATCTTATAAGTTTATTTTCTGGTGCTGGTGGATTAGATTTAGGCTTTATTAAAGCGGGATTTAATGTTTTAGTGGC
TAATGAATATGACAAAAGAATATGGGAAACCTATGAAAAGAATTATGAAACAAGGCTGATTAAAGATGATATAACTAATG
TGAGGTCTAATGAACTTCCAGATTGTGACGGTTTAATAGGCGGCCCTCCTTGTCAGTCTTGGAGTGAAGCCGGTTCTCTA
AAAGGGATAGAGGATCCAAGAGGGCAGTTGTTTTATGAATATATAAGGATTTTACGAGATAAAAGTCCTAAATTTTTTCT
TGCGGAAAATGTAAAAGGAATGATGGCAAAAAGACATAATAAAGCTGTTCAAAGTATAATTTCACAATTTAACAAAGCTG
GATATGATGTTTTTATACACTTATTAAACGCTTCTGATTATGGTGTTGCTCAAGATAGAAAAAGAATTTTTTATGTTGGC
TTCAGAAAAGATTTAAACATATGTTTTGATAATCCTCCAAAAAAATTCAATTATAAGCCTACCCTGAAAGATGCTATATA
TGATTTGAAAGATACAGCAATACCTTCTTTGAATAGAAACAAGACTAATGGTGATAATTGTAAGGTTACAAATCACGAAT
ACTTTACTGGTTCTTATTCCCCTATATTTATGAGTAGAAATCGTGTTAGGTCGTGGAATGAGCCGGCCTTTACAATTCAA
GCTTCTGGAAGACAATGTCAATTGCACCCACAAGCCCCTAAAATGCTTAAAATTAGTAAGGATAAACATATATTTGTACC
AGGTAAGGAAAAACTGTATAGAAGATTGACAGTAAGAGAATGTGCTAGAATTCAGGGATTTCCCGATGATTTTATTTTCT
ACTATAAAGATGTGAATGATGGATATAAGATGATAGGAAATGCAGTTCCAATCAATTTAGCATTTGTAATCGCAATGAAT
ATAAAAAGTCATATAATGTCAGTATATTGTTAG

Upstream 100 bases:

>100_bases
TGTTACATGTTCTAGGTTGGTTACTTATTTGTGGTGTTGTCACTTGTTGATATATTACATATAACAAAGAAACAAAGGAG
AATAATAAGAAAGGGATTAA

Downstream 100 bases:

>100_bases
TTCTCTCCCCTGCTATTATCTGGCAGGGGCTTTTATGTGGGTACATATTCTGGGTGTATTAATTATTTGTGGTGTTGTTA
CTTGTTGCTATATTACATAC

Product: cytosine-specific methyltransferase

Products: NA

Alternate protein names: M.HaeIII; Cytosine-specific methyltransferase HaeIII [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MDLISLFSGAGGLDLGFIKAGFNVLVANEYDKRIWETYEKNYETRLIKDDITNVRSNELPDCDGLIGGPPCQSWSEAGSL
KGIEDPRGQLFYEYIRILRDKSPKFFLAENVKGMMAKRHNKAVQSIISQFNKAGYDVFIHLLNASDYGVAQDRKRIFYVG
FRKDLNICFDNPPKKFNYKPTLKDAIYDLKDTAIPSLNRNKTNGDNCKVTNHEYFTGSYSPIFMSRNRVRSWNEPAFTIQ
ASGRQCQLHPQAPKMLKISKDKHIFVPGKEKLYRRLTVRECARIQGFPDDFIFYYKDVNDGYKMIGNAVPINLAFVIAMN
IKSHIMSVYC

Sequences:

>Translated_330_residues
MDLISLFSGAGGLDLGFIKAGFNVLVANEYDKRIWETYEKNYETRLIKDDITNVRSNELPDCDGLIGGPPCQSWSEAGSL
KGIEDPRGQLFYEYIRILRDKSPKFFLAENVKGMMAKRHNKAVQSIISQFNKAGYDVFIHLLNASDYGVAQDRKRIFYVG
FRKDLNICFDNPPKKFNYKPTLKDAIYDLKDTAIPSLNRNKTNGDNCKVTNHEYFTGSYSPIFMSRNRVRSWNEPAFTIQ
ASGRQCQLHPQAPKMLKISKDKHIFVPGKEKLYRRLTVRECARIQGFPDDFIFYYKDVNDGYKMIGNAVPINLAFVIAMN
IKSHIMSVYC
>Mature_330_residues
MDLISLFSGAGGLDLGFIKAGFNVLVANEYDKRIWETYEKNYETRLIKDDITNVRSNELPDCDGLIGGPPCQSWSEAGSL
KGIEDPRGQLFYEYIRILRDKSPKFFLAENVKGMMAKRHNKAVQSIISQFNKAGYDVFIHLLNASDYGVAQDRKRIFYVG
FRKDLNICFDNPPKKFNYKPTLKDAIYDLKDTAIPSLNRNKTNGDNCKVTNHEYFTGSYSPIFMSRNRVRSWNEPAFTIQ
ASGRQCQLHPQAPKMLKISKDKHIFVPGKEKLYRRLTVRECARIQGFPDDFIFYYKDVNDGYKMIGNAVPINLAFVIAMN
IKSHIMSVYC

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the HaeIII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=205, Percent_Identity=30.2439024390244, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 37874; Mature: 37874

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLISLFSGAGGLDLGFIKAGFNVLVANEYDKRIWETYEKNYETRLIKDDITNVRSNELP
CCHHHHHCCCCCCCCCEEECCCEEEEECCHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCC
DCDGLIGGPPCQSWSEAGSLKGIEDPRGQLFYEYIRILRDKSPKFFLAENVKGMMAKRHN
CCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
KAVQSIISQFNKAGYDVFIHLLNASDYGVAQDRKRIFYVGFRKDLNICFDNPPKKFNYKP
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCC
TLKDAIYDLKDTAIPSLNRNKTNGDNCKVTNHEYFTGSYSPIFMSRNRVRSWNEPAFTIQ
CHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEECCCCCEEEECCCCCCCCCCCEEEEE
ASGRQCQLHPQAPKMLKISKDKHIFVPGKEKLYRRLTVRECARIQGFPDDFIFYYKDVND
ECCCEEEECCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GYKMIGNAVPINLAFVIAMNIKSHIMSVYC
CHHHHCCCCCEEEEEEEEECHHHHHHHHCC
>Mature Secondary Structure
MDLISLFSGAGGLDLGFIKAGFNVLVANEYDKRIWETYEKNYETRLIKDDITNVRSNELP
CCHHHHHCCCCCCCCCEEECCCEEEEECCHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCC
DCDGLIGGPPCQSWSEAGSLKGIEDPRGQLFYEYIRILRDKSPKFFLAENVKGMMAKRHN
CCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
KAVQSIISQFNKAGYDVFIHLLNASDYGVAQDRKRIFYVGFRKDLNICFDNPPKKFNYKP
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCC
TLKDAIYDLKDTAIPSLNRNKTNGDNCKVTNHEYFTGSYSPIFMSRNRVRSWNEPAFTIQ
CHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEECCCCCEEEECCCCCCCCCCCEEEEE
ASGRQCQLHPQAPKMLKISKDKHIFVPGKEKLYRRLTVRECARIQGFPDDFIFYYKDVND
ECCCEEEECCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GYKMIGNAVPINLAFVIAMNIKSHIMSVYC
CHHHHCCCCCEEEEEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3248732; 1932026; 7606780 [H]