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The map label for this gene is maeB [H]

Identifier: 167625764

GI number: 167625764

Start: 4681120

End: 4682370

Strand: Reverse

Name: maeB [H]

Synonym: Shal_3859

Alternate gene names: 167625764

Gene position: 4682370-4681120 (Counterclockwise)

Preceding gene: 167625765

Following gene: 167625763

Centisome position: 89.58

GC content: 45.8

Gene sequence:

>1251_bases
ATGGCAGATCTTCGTCAACAAGCCCTCGATTATCATGAATTCCCCGTTCCAGGCAAAACAGCAGTTTGCCTAACTAAACC
CGCAGAAACTAGCATGGACCTCGCACTGGCTTATAGCCCCGGCGTGGCAGAGCCTGTACGTGAAATTGCGGCGAATCCAG
ACAATGCTTACCGTTATACAGGTAAAGGCAACACTGTTGCAGTTATCTCTAACGGTACCGCAATTTTAGGTCTAGGTAAC
TTAGGCCCGTTGGCATCTAAGCCTGTTATGGAAGGCAAGGCTTTGCTTTTCAAACACTTTGCAAATATTGATGCTACTGA
TATTGAAGTAAAGCACAGAACATCTGAAGAGTTCATCAACACCGTTGAAGCGATTGCTGATACATTTGGTGGTATCAACC
TTGAAGATATCAAGGCGCCAGAGTGTTTTGAAATTGAAAAGGCACTGATTGAGCTTTGTAATGTGCCTGTATTCCACGAT
GATCAGCATGGCACTGCAATCGTAACTGCTGCGGGTATGATTAATGCGCTTGAAATTCAGGGCAAATCTATCGAAGAAGC
CGTATTTGTTTGTATGGGTGCCGGTGCAGCCGCGATCGCCTGTATGACGATGATTGTAAATTGCGGTGCTCAACGCGAAA
ACGTCTACATGTTGGATAGAAAAGGCGTTATCCATACTCGTCGTGAAGACATTAACGAGTATAAAGCGCTATTTGCTAAT
AATACTGACAAGCGTACGCTGCAAGATGTGATTAAAGGTGCTGACGTATTCTTAGGTCTTTCTGGGCCTGACGTTTTAGG
CGCCGAAGATGTTGCAATGATGGCTGATAAGCCAGTGATTTTTGCCTGTTCTAACCCAGATCCTGAAATTAAACCTGAAC
TTGCTCATGATGTTCGCAAAGATTTAATCATGGGTACGGGCCGTAGCGACTACCCTAACCAAGTTAACAACGTGCTTTGT
TTCCCATTCATTTTCCGTGGCGCACTCGATGTGCGTGCTTCACGGATCAATGATGAGATGAAGATTGCGGCTGTACACGC
GATTGCAGCTTTGGCGAAAGAGCCTGTACCAGCAGCTGTACTCGCTGCATATCCAAATGTTGACTCATTAGAATTTGGCC
CCGATTACGTGCTCCCTAAGCCAACTGATGCACGTCTTCTACCTAATGTGGCTAAAGCCGTGGCATTGGCTGCGATTGAA
TCAGGCGTTGCGGTAATTAAAGAGCTTCCTGAAGGCTATATGGAGTCTTAA

Upstream 100 bases:

>100_bases
AAAATGCCTTGAAAATGTATCTTTATTGAGCAGCAAGATTAACCTTTTAGTTGTCGACCTTTCGCTCAGGATATTATTAG
TCGCACTCAGGATTAGAAAA

Downstream 100 bases:

>100_bases
GGACTCTGCTTATCAGAAAAAAAGGGACCCAAGGGTCCCTTTTTTGTTGCCGAGCGTTTTTCGGTTACTGTATAACTGAA
TGTTATTAAGCTAAGGCTGG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYTGKGNTVAVISNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFINTVEAIADTFGGINLEDIKAPECFEIEKALIELCNVPVFHD
DQHGTAIVTAAGMINALEIQGKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVAMMADKPVIFACSNPDPEIKPELAHDVRKDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASRINDEMKIAAVHAIAALAKEPVPAAVLAAYPNVDSLEFGPDYVLPKPTDARLLPNVAKAVALAAIE
SGVAVIKELPEGYMES

Sequences:

>Translated_416_residues
MADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYTGKGNTVAVISNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFINTVEAIADTFGGINLEDIKAPECFEIEKALIELCNVPVFHD
DQHGTAIVTAAGMINALEIQGKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVAMMADKPVIFACSNPDPEIKPELAHDVRKDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASRINDEMKIAAVHAIAALAKEPVPAAVLAAYPNVDSLEFGPDYVLPKPTDARLLPNVAKAVALAAIE
SGVAVIKELPEGYMES
>Mature_415_residues
ADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYTGKGNTVAVISNGTAILGLGNL
GPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFINTVEAIADTFGGINLEDIKAPECFEIEKALIELCNVPVFHDD
QHGTAIVTAAGMINALEIQGKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFANN
TDKRTLQDVIKGADVFLGLSGPDVLGAEDVAMMADKPVIFACSNPDPEIKPELAHDVRKDLIMGTGRSDYPNQVNNVLCF
PFIFRGALDVRASRINDEMKIAAVHAIAALAKEPVPAAVLAAYPNVDSLEFGPDYVLPKPTDARLLPNVAKAVALAAIES
GVAVIKELPEGYMES

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=354, Percent_Identity=26.8361581920904, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI62420880, Length=354, Percent_Identity=26.8361581920904, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI239049447, Length=354, Percent_Identity=26.8361581920904, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4505143, Length=344, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4505145, Length=373, Percent_Identity=26.0053619302949, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788806, Length=404, Percent_Identity=60.8910891089109, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI87081919, Length=300, Percent_Identity=27, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17537199, Length=367, Percent_Identity=25.8855585831063, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=327, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707242, Length=402, Percent_Identity=26.3681592039801, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707238, Length=402, Percent_Identity=26.3681592039801, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707240, Length=393, Percent_Identity=26.2086513994911, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281362672, Length=368, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281362674, Length=368, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356279, Length=368, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281363503, Length=357, Percent_Identity=22.96918767507, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363505, Length=357, Percent_Identity=22.96918767507, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78707236, Length=357, Percent_Identity=22.96918767507, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78707232, Length=357, Percent_Identity=22.96918767507, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646388, Length=366, Percent_Identity=27.3224043715847, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646386, Length=366, Percent_Identity=27.3224043715847, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44622; Mature: 44490

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYT
CCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEE
GKGNTVAVISNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFIN
CCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
TVEAIADTFGGINLEDIKAPECFEIEKALIELCNVPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCEEEEEEC
GKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVAMMADKPVIFACSNPDPEIKPELAHDVRK
CCCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
DLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASRINDEMKIAAVHAIAALAKEPVPAAV
HHEECCCCCCCCHHHHCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
LAAYPNVDSLEFGPDYVLPKPTDARLLPNVAKAVALAAIESGVAVIKELPEGYMES
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
ADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYT
CCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEE
GKGNTVAVISNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFIN
CCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
TVEAIADTFGGINLEDIKAPECFEIEKALIELCNVPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCEEEEEEC
GKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVAMMADKPVIFACSNPDPEIKPELAHDVRK
CCCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
DLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASRINDEMKIAAVHAIAALAKEPVPAAV
HHEECCCCCCCCHHHHCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
LAAYPNVDSLEFGPDYVLPKPTDARLLPNVAKAVALAAIESGVAVIKELPEGYMES
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]