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The map label for this gene is opuD [H]

Identifier: 167625666

GI number: 167625666

Start: 4565509

End: 4567212

Strand: Reverse

Name: opuD [H]

Synonym: Shal_3761

Alternate gene names: 167625666

Gene position: 4567212-4565509 (Counterclockwise)

Preceding gene: 167625667

Following gene: 167625665

Centisome position: 87.38

GC content: 46.54

Gene sequence:

>1704_bases
ATGGCTGACGATGACAAAAGCGAATACTTAATGTCGAATTTGACACAATCTGTTGATCGTAAATCAACAGATGAAAACTA
CCTTGCAGCCCATCCCCCTGAACAAAGTGATGCGTCAACCAAGGTAAATCCCTTCTCCAGTCCAGTACTCTGGTTAAGTG
GCGGCTTTTTAGCTCTCTTTGTACTGCTTGCTGTTTTCGATACCTCAGCTCTGTCCAACATCATTCAAGTTGGATTCTCT
AAAGTGACCCAATGGTTTGGCCCATTTTGGCAAATTCTTTTGCTGGCTAACTTTATTATTGGTCTCTACTTAGCCTTTGC
TAGAACTGGCGACGTTAAGCTCGGTAATCGCAGCACCCCAGAAATTGATACCTTCAAATGGTTGGCGATTGTTCTGTGTA
CCCTGTTAGCCGGTGGTGGTGTATTTTGGGCTGCAGCTGAGCCTATCGCTCATTTTGTTTCCTCGCCACCTTTTTACGGT
GAAGCAGAGGGGCGCACGCTGGCGATCAATGCCTTATCTCAATCTTTTCTGCATTGGGGGTTTCTTGCATGGGCGATACT
CGGTAGCTTAGCCGCGATTGTTTTAATGCATCTACACTACGACAAAGGTTTACCATTGCAACCTCGCACTTTGTTGTATC
CCATTTTTGGCGATAAAGCACTTAACGGTTGGATTGGTACCTTAACCGACACATTTTGTATTATTGCTGTGGCCGCCGGT
ACGATTGGTCCAATTGGCTTTCTTGGTCTGCAAATTAGCTATGCGCTCAATGCGCTTTTTGGTGTGCAAGATACCTTAAT
CACCCAGAGTTTGGTGGTACTGTTTGCCATCGCTATGTATACCTTATCGGCATTAAGTGGCCTAAGCCGCGGCATTCAGC
TAATAAGCCGCTACAACATTTTACTTTCTGCTGCACTGATCATCTTCATTCTACTATTTGGCCCTACCAGCTTCATCATC
GATGGCTACATGCAGGGCGTGGCTAAAATGGTCAATAACTTTGTGCCGATGTCACTCTACCGAGGCGATGCTAATTGGCT
AAGTTGGTGGACGGTGTTTTTCTGGGGCTGGTTTATTGGCTATGGTCCGATGATGGCGATCTTCATTGCGCGAATTTCTC
GCGGCCGCACGATTCGTCAATTGATCCTGTCTATTTCTATTATTGCACCACTCATCACCTGTTTCTGGTTTAGTGTTGTC
GGTGGATCTGGCCTTGCGTTCGAGTTAGCGGAGCCCGGTGTTATCTCGACAGCATTTGAAGGATTTAATTTACCTGGCGT
GTTGCTGGCTATTACCTCACAACTGCCAATGCCGTCGCTGATTGCAGTACTGTTTTTAATCCTAACGACCACATTTATCG
TCACTACAGGTGATTCGATGACTTATACCATTAGTGTGGTCGTGACAGGTTCCACCGAGCCTAGTGGCGCAATGCGTGCG
TTTTGGGGAATATTAATGGGACTGGTTGCTATCGCATTGATCTCGATGGGCTCTGGTGGCATTTCCGCCCTACAGTCGTT
TATTGTGATCACTGCCGTTCCGGTGTCATTTGTTTTGCTACCATCACTGTGGCAAGCACCCAAAATGGCTAAAGCGATGG
CGGAGCAGCAATTTGGTAAGCCATCACTCAATCATGCGCAAGCAAGCCATTCACAAACAGAGCATTCACAAACAAAGCAT
TCGCAAGCAAAGCAATCCGGATAA

Upstream 100 bases:

>100_bases
CAGCCTCAACCTTTACAAAGTCACTCAATGTCTTTGCGATAGCCGAGGAAAATTCACCGACTCAGCTTAAAATATATCTT
AATAAACAGACTTATATTTA

Downstream 100 bases:

>100_bases
GTGGGTTAAAGTAAACGTTTACTTTAAAGCCCCGATGCTTGTTAGTATCGGGGCTATTGCTTATGTACTGACCCACACCG
CCGCCTCGGCAGTATAGCCT

Product: BCCT transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MADDDKSEYLMSNLTQSVDRKSTDENYLAAHPPEQSDASTKVNPFSSPVLWLSGGFLALFVLLAVFDTSALSNIIQVGFS
KVTQWFGPFWQILLLANFIIGLYLAFARTGDVKLGNRSTPEIDTFKWLAIVLCTLLAGGGVFWAAAEPIAHFVSSPPFYG
EAEGRTLAINALSQSFLHWGFLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDKALNGWIGTLTDTFCIIAVAAG
TIGPIGFLGLQISYALNALFGVQDTLITQSLVVLFAIAMYTLSALSGLSRGIQLISRYNILLSAALIIFILLFGPTSFII
DGYMQGVAKMVNNFVPMSLYRGDANWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVV
GGSGLAFELAEPGVISTAFEGFNLPGVLLAITSQLPMPSLIAVLFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRA
FWGILMGLVAIALISMGSGGISALQSFIVITAVPVSFVLLPSLWQAPKMAKAMAEQQFGKPSLNHAQASHSQTEHSQTKH
SQAKQSG

Sequences:

>Translated_567_residues
MADDDKSEYLMSNLTQSVDRKSTDENYLAAHPPEQSDASTKVNPFSSPVLWLSGGFLALFVLLAVFDTSALSNIIQVGFS
KVTQWFGPFWQILLLANFIIGLYLAFARTGDVKLGNRSTPEIDTFKWLAIVLCTLLAGGGVFWAAAEPIAHFVSSPPFYG
EAEGRTLAINALSQSFLHWGFLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDKALNGWIGTLTDTFCIIAVAAG
TIGPIGFLGLQISYALNALFGVQDTLITQSLVVLFAIAMYTLSALSGLSRGIQLISRYNILLSAALIIFILLFGPTSFII
DGYMQGVAKMVNNFVPMSLYRGDANWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVV
GGSGLAFELAEPGVISTAFEGFNLPGVLLAITSQLPMPSLIAVLFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRA
FWGILMGLVAIALISMGSGGISALQSFIVITAVPVSFVLLPSLWQAPKMAKAMAEQQFGKPSLNHAQASHSQTEHSQTKH
SQAKQSG
>Mature_566_residues
ADDDKSEYLMSNLTQSVDRKSTDENYLAAHPPEQSDASTKVNPFSSPVLWLSGGFLALFVLLAVFDTSALSNIIQVGFSK
VTQWFGPFWQILLLANFIIGLYLAFARTGDVKLGNRSTPEIDTFKWLAIVLCTLLAGGGVFWAAAEPIAHFVSSPPFYGE
AEGRTLAINALSQSFLHWGFLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDKALNGWIGTLTDTFCIIAVAAGT
IGPIGFLGLQISYALNALFGVQDTLITQSLVVLFAIAMYTLSALSGLSRGIQLISRYNILLSAALIIFILLFGPTSFIID
GYMQGVAKMVNNFVPMSLYRGDANWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVG
GSGLAFELAEPGVISTAFEGFNLPGVLLAITSQLPMPSLIAVLFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRAF
WGILMGLVAIALISMGSGGISALQSFIVITAVPVSFVLLPSLWQAPKMAKAMAEQQFGKPSLNHAQASHSQTEHSQTKHS
QAKQSG

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=530, Percent_Identity=31.3207547169811, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788102, Length=436, Percent_Identity=26.8348623853211, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1786224, Length=464, Percent_Identity=26.2931034482759, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 61334; Mature: 61203

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDDKSEYLMSNLTQSVDRKSTDENYLAAHPPEQSDASTKVNPFSSPVLWLSGGFLALF
CCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHCCCHHHHH
VLLAVFDTSALSNIIQVGFSKVTQWFGPFWQILLLANFIIGLYLAFARTGDVKLGNRSTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
EIDTFKWLAIVLCTLLAGGGVFWAAAEPIAHFVSSPPFYGEAEGRTLAINALSQSFLHWG
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
FLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDKALNGWIGTLTDTFCIIAVAAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHC
TIGPIGFLGLQISYALNALFGVQDTLITQSLVVLFAIAMYTLSALSGLSRGIQLISRYNI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSAALIIFILLFGPTSFIIDGYMQGVAKMVNNFVPMSLYRGDANWLSWWTVFFWGWFIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHH
YGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVGGSGLAFELAEPGVISTAFE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHC
GFNLPGVLLAITSQLPMPSLIAVLFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRA
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCCHHHHH
FWGILMGLVAIALISMGSGGISALQSFIVITAVPVSFVLLPSLWQAPKMAKAMAEQQFGK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
PSLNHAQASHSQTEHSQTKHSQAKQSG
CCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADDDKSEYLMSNLTQSVDRKSTDENYLAAHPPEQSDASTKVNPFSSPVLWLSGGFLALF
CCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHCCCHHHHH
VLLAVFDTSALSNIIQVGFSKVTQWFGPFWQILLLANFIIGLYLAFARTGDVKLGNRSTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
EIDTFKWLAIVLCTLLAGGGVFWAAAEPIAHFVSSPPFYGEAEGRTLAINALSQSFLHWG
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
FLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDKALNGWIGTLTDTFCIIAVAAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHC
TIGPIGFLGLQISYALNALFGVQDTLITQSLVVLFAIAMYTLSALSGLSRGIQLISRYNI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSAALIIFILLFGPTSFIIDGYMQGVAKMVNNFVPMSLYRGDANWLSWWTVFFWGWFIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHH
YGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVGGSGLAFELAEPGVISTAFE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHC
GFNLPGVLLAITSQLPMPSLIAVLFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRA
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCCHHHHH
FWGILMGLVAIALISMGSGGISALQSFIVITAVPVSFVLLPSLWQAPKMAKAMAEQQFGK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
PSLNHAQASHSQTEHSQTKHSQAKQSG
CCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]