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The map label for this gene is dusA [H]

Identifier: 167625570

GI number: 167625570

Start: 4462801

End: 4463787

Strand: Reverse

Name: dusA [H]

Synonym: Shal_3664

Alternate gene names: 167625570

Gene position: 4463787-4462801 (Counterclockwise)

Preceding gene: 167625571

Following gene: 167625569

Centisome position: 85.4

GC content: 46.5

Gene sequence:

>987_bases
ATGTCAGTTAGACCAATCAACCGCACCTTCTCAATCGCTCCCATGCTGGATTGGACCGACAGGCATTATCGTTATTTTGC
TCGTTTGATGTCTTCGCAGGTGCTGCTATATACCGAAATGGTAACGACTGGCGCTATCTTGCATGGACGCGGTGATTACC
TCGCTTATAACCAAGAAGAGCATCCACTCGCATTGCAACTGGGTGGATCAAATCCAGCCGATCTAGCGGCTTGTGCCAAG
CTCGCTGCCGAACGGGGTTATGATGAGGTTAACCTTAATGTGGGTTGCCCATCGGATAGAGTACAGAATGGTCGTTTTGG
TGCCTGCCTAATGGCTGAGCCTGAACTTGTTGCCGAGTGTGTGTCGGCAATGAAGCAAGTCGTTGACATCCCTATTACGG
TTAAAACCCGTATCGGCATTGACGAGCAAGATAGCTATGAGTTTTTAACTCAGTTTATCGATACTGTTAAAGCGGCGGGT
TGTGAGGAGTTTACCATTCATGCTCGTAAGGCATGGCTCCAAGGCTTAAGTCCAAAAGAGAATCGCGAGATCCCGCCATT
AGATTATGAGCGTGTGTATCAACTTAAGCGTGATTATCCGCAGCTGAACATCAGTATCAATGGTGGCATTAAAACGCTAG
AAGAGTGTCAAACACACCTGCAGCACCTTGATGGCGTTATGGTTGGCCGCGAAGCTTACCAAAACCCATATATTTTGGCT
GAAGTCGATCAAAAGCTATGTGGTATAGATACGCCAGTAATAAGTCGAGATGCTGTTTTGGAGAAACTACTTCCTTATAT
AGAGAAACATCTACAGCAAGGTGGCCGCTTAAATCATATCACTCGCCACATCATAGGCTTGTATCAAGGTGAAGTGGGTT
CGCGTGTATGGCGTCGTTATCTAAGTGAAAATGCCCATAAACAAGGCGCTGGAATTGAAGTGGTAACTCAAGCTAGAGCC
GAAATGAACGGCATTGTGAGCTTATAG

Upstream 100 bases:

>100_bases
CTATCATGGCTTGGACAGTGGAGTGAGAGTTTTACTAAATAGATACTCCCCCTTAGATTTCGGTGAGTAAAGTCATTATA
AAGAGTTGGATAAATAGTTT

Downstream 100 bases:

>100_bases
TTACTTTGTTTAGTGAAATTCACCAGAGCATGGTGAGTTTCACTAATATCTTTTATTGTTTAAATATTGCTCTGCCATTC
ACTATTCCCATCTAGAGTTA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSVRPINRTFSIAPMLDWTDRHYRYFARLMSSQVLLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPADLAACAK
LAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVSAMKQVVDIPITVKTRIGIDEQDSYEFLTQFIDTVKAAG
CEEFTIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPQLNISINGGIKTLEECQTHLQHLDGVMVGREAYQNPYILA
EVDQKLCGIDTPVISRDAVLEKLLPYIEKHLQQGGRLNHITRHIIGLYQGEVGSRVWRRYLSENAHKQGAGIEVVTQARA
EMNGIVSL

Sequences:

>Translated_328_residues
MSVRPINRTFSIAPMLDWTDRHYRYFARLMSSQVLLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPADLAACAK
LAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVSAMKQVVDIPITVKTRIGIDEQDSYEFLTQFIDTVKAAG
CEEFTIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPQLNISINGGIKTLEECQTHLQHLDGVMVGREAYQNPYILA
EVDQKLCGIDTPVISRDAVLEKLLPYIEKHLQQGGRLNHITRHIIGLYQGEVGSRVWRRYLSENAHKQGAGIEVVTQARA
EMNGIVSL
>Mature_327_residues
SVRPINRTFSIAPMLDWTDRHYRYFARLMSSQVLLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPADLAACAKL
AAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVSAMKQVVDIPITVKTRIGIDEQDSYEFLTQFIDTVKAAGC
EEFTIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPQLNISINGGIKTLEECQTHLQHLDGVMVGREAYQNPYILAE
VDQKLCGIDTPVISRDAVLEKLLPYIEKHLQQGGRLNHITRHIIGLYQGEVGSRVWRRYLSENAHKQGAGIEVVTQARAE
MNGIVSL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=26.271186440678, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=25.1046025104602, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=25.1046025104602, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40807366, Length=238, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=71.2903225806452, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.0162601626016, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788462, Length=224, Percent_Identity=29.0178571428571, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=172, Percent_Identity=32.5581395348837, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=234, Percent_Identity=25.2136752136752, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=25.3164556962025, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=253, Percent_Identity=26.8774703557312, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920448, Length=253, Percent_Identity=26.8774703557312, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921524, Length=234, Percent_Identity=23.9316239316239, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=24.6527777777778, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36886; Mature: 36754

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRPINRTFSIAPMLDWTDRHYRYFARLMSSQVLLYTEMVTTGAILHGRGDYLAYNQEE
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCEEEECCCCEEEECCCC
HPLALQLGGSNPADLAACAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAEC
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCEEEECCHHHHHHH
VSAMKQVVDIPITVKTRIGIDEQDSYEFLTQFIDTVKAAGCEEFTIHARKAWLQGLSPKE
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
NREIPPLDYERVYQLKRDYPQLNISINGGIKTLEECQTHLQHLDGVMVGREAYQNPYILA
CCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
EVDQKLCGIDTPVISRDAVLEKLLPYIEKHLQQGGRLNHITRHIIGLYQGEVGSRVWRRY
EHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LSENAHKQGAGIEVVTQARAEMNGIVSL
HHCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVRPINRTFSIAPMLDWTDRHYRYFARLMSSQVLLYTEMVTTGAILHGRGDYLAYNQEE
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCEEEECCCCEEEECCCC
HPLALQLGGSNPADLAACAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAEC
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCEEEECCHHHHHHH
VSAMKQVVDIPITVKTRIGIDEQDSYEFLTQFIDTVKAAGCEEFTIHARKAWLQGLSPKE
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
NREIPPLDYERVYQLKRDYPQLNISINGGIKTLEECQTHLQHLDGVMVGREAYQNPYILA
CCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
EVDQKLCGIDTPVISRDAVLEKLLPYIEKHLQQGGRLNHITRHIIGLYQGEVGSRVWRRY
EHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LSENAHKQGAGIEVVTQARAEMNGIVSL
HHCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]