Click here to switch to the map view.

The map label for this gene is hldE

Identifier: 167625458

GI number: 167625458

Start: 4329709

End: 4331139

Strand: Reverse

Name: hldE

Synonym: Shal_3550

Alternate gene names: 167625458

Gene position: 4331139-4329709 (Counterclockwise)

Preceding gene: 167625460

Following gene: 167625457

Centisome position: 82.86

GC content: 50.17

Gene sequence:

>1431_bases
ATGAAGATTTCTCTGCCAGCATTTGAAAAAGCCAAAGTTCTCGTTGTCGGTGACGTGATGTTAGATCGCTATTGGGCGGG
GCCTACAGCGCGGATCTCACCAGAAGCGCCTGTACCTGTCGTAAAGGTTGAGCAACTTGAGGATAGACCCGGCGGCGCGG
CGAACGTGGCGATCAATATTGCGACTTTAGGTGGCAGTGCAAGCCTTGCGGGTATTGTCGGTGAAGATGAAACCGCTGAT
GCGCTGACCTTGGGTATTCAAACCCTTGGGGTTGAGCCTAACTGGCATAAAGTGGCGGGTAAGCCGACCATCACTAAGCT
ACGTGTGATGTCACGTAACCAGCAGTTACTGCGTTTAGACTTTGAAGAAAGTTATACCCAAGCTGAGAGTGAGGCGCTGC
TTGCTAAAACGCTACCTGAGCTAGATAAGGTCGACGTGGCAGTGTTATCTGATTACGCTAAAGGCGCAATTATTTCTCCA
CAAAGCTTTATCCAAGCGGCTAACGCTAAAGGCGTAAAAGTGTTAGTCGATCCTAAGGGCAGCGACTTTTCAAAGTATCG
CGGCGCTTACCTGCTGACGCCAAATATGAGTGAGTTTGAAGTGGTCGTCGGCAAGGTTGAGTCGGAAGCTGACTTAGTGG
CTAAGGCGCAAGGCTTGTTAGAAGCTTATGACTTTACTGCCATGCTAGTGACTCGCTCTGAAAAAGGCATGACCTTAATT
ACTCGCGATCAACCTGAGCTACATATTCCTACCGTGGCTCGTGAAGTTTTTGATGTCACTGGTGCGGGTGATACGGTGAT
TTCAGCATTGGCTACCGCGATTGCTGCGGGTAGTGACTTGCCACAAGCCTGTGCTATTGCGAACACGGCGGCGGGCATTG
TTGTGGCTAAGCTTGGCACATCGACAGTGACCCGACTCGAGCTTATCGAAGCGTTATCTTTAAGCCATGGTGAGACAGGT
TTTGGCGCGGTCAGTGAAGATCAACTTGCCTATGCTCTTGAGCAGGCAAAGCTTCGCGGTGAACGTGTGGTGATGACCAA
TGGCTGTTTTGATATCTTGCATGCTGGCCATGTGAGTTACCTGCAACAGGCTCGCGCCTTAGGCGATCGCTTAATTGTAG
CGGTTAACACTGACGCTTCGGTTAAGCGCCTTAAAGGTGAAGGACGCCCTGTTAACACCGAAGATAGACGTATGACAGTA
CTTGCCGCGCTTGCATCTGTGGATTGGGTGGTACCCTTCACTGAAGATACACCGCAACGCATCATCTCGCGCTTACTACC
TGACTTACTGGTTAAAGGTGGCGATTATAAGGTGGAAGATATTGCTGGTGGCGCAGAGGTGATGGCTGCAGGTGGTCAGG
TCAAAGTGTTAGGCTTTGAAGATGGCATCTCAACCACGGCGATTATCCAAAATATCATGGCTAATCAGTAA

Upstream 100 bases:

>100_bases
GTAATACTCGAATGCATTGAAAATTTACTCATTATTCTAACTCAAAAGTGATTAAAATTAGGTACAATCAGAATTAAATT
TTGTGAAAGATATAGGCGTT

Downstream 100 bases:

>100_bases
CCATTGATGATTAGTCGATAGTTAGTGACGATAACCCCAAATGATAAGCTCGCTCGCCGCGACGGTTGCAACGACTTCTG
CAACTGCAATTTTAGCTACA

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINIATLGGSASLAGIVGEDETAD
ALTLGIQTLGVEPNWHKVAGKPTITKLRVMSRNQQLLRLDFEESYTQAESEALLAKTLPELDKVDVAVLSDYAKGAIISP
QSFIQAANAKGVKVLVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLEAYDFTAMLVTRSEKGMTLI
TRDQPELHIPTVAREVFDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGTSTVTRLELIEALSLSHGETG
FGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNTEDRRMTV
LAALASVDWVVPFTEDTPQRIISRLLPDLLVKGGDYKVEDIAGGAEVMAAGGQVKVLGFEDGISTTAIIQNIMANQ

Sequences:

>Translated_476_residues
MKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINIATLGGSASLAGIVGEDETAD
ALTLGIQTLGVEPNWHKVAGKPTITKLRVMSRNQQLLRLDFEESYTQAESEALLAKTLPELDKVDVAVLSDYAKGAIISP
QSFIQAANAKGVKVLVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLEAYDFTAMLVTRSEKGMTLI
TRDQPELHIPTVAREVFDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGTSTVTRLELIEALSLSHGETG
FGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNTEDRRMTV
LAALASVDWVVPFTEDTPQRIISRLLPDLLVKGGDYKVEDIAGGAEVMAAGGQVKVLGFEDGISTTAIIQNIMANQ
>Mature_476_residues
MKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINIATLGGSASLAGIVGEDETAD
ALTLGIQTLGVEPNWHKVAGKPTITKLRVMSRNQQLLRLDFEESYTQAESEALLAKTLPELDKVDVAVLSDYAKGAIISP
QSFIQAANAKGVKVLVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLEAYDFTAMLVTRSEKGMTLI
TRDQPELHIPTVAREVFDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGTSTVTRLELIEALSLSHGETG
FGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNTEDRRMTV
LAALASVDWVVPFTEDTPQRIISRLLPDLLVKGGDYKVEDIAGGAEVMAAGGQVKVLGFEDGISTTAIIQNIMANQ

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Homo sapiens, GI4505651, Length=132, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI296841136, Length=132, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789432, Length=476, Percent_Identity=59.8739495798319, Blast_Score=570, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_SHEHH (B0TTF2)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675752.1
- ProteinModelPortal:   B0TTF2
- SMR:   B0TTF2
- GeneID:   5905942
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3550
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- BioCyc:   SHAL458817:SHAL_3550-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 50460; Mature: 50460

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINI
CCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEEHHCCCCCCCEEEEEEE
ATLGGSASLAGIVGEDETADALTLGIQTLGVEPNWHKVAGKPTITKLRVMSRNQQLLRLD
EEECCCCCEEEECCCCCCCCCEEEEHEEECCCCCCCEECCCCCCEEEEECCCCCEEEEEE
FEESYTQAESEALLAKTLPELDKVDVAVLSDYAKGAIISPQSFIQAANAKGVKVLVDPKG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECHHHHHHHCCCCCEEEEECCCC
SDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLEAYDFTAMLVTRSEKGMTLI
CCHHHHCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEE
TRDQPELHIPTVAREVFDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGT
ECCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCC
STVTRLELIEALSLSHGETGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSY
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
LQQARALGDRLIVAVNTDASVKRLKGEGRPVNTEDRRMTVLAALASVDWVVPFTEDTPQR
HHHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCHHH
IISRLLPDLLVKGGDYKVEDIAGGAEVMAAGGQVKVLGFEDGISTTAIIQNIMANQ
HHHHHHHHHHHCCCCEEEHHHCCCHHEEECCCEEEEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINI
CCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEEHHCCCCCCCEEEEEEE
ATLGGSASLAGIVGEDETADALTLGIQTLGVEPNWHKVAGKPTITKLRVMSRNQQLLRLD
EEECCCCCEEEECCCCCCCCCEEEEHEEECCCCCCCEECCCCCCEEEEECCCCCEEEEEE
FEESYTQAESEALLAKTLPELDKVDVAVLSDYAKGAIISPQSFIQAANAKGVKVLVDPKG
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECHHHHHHHCCCCCEEEEECCCC
SDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLEAYDFTAMLVTRSEKGMTLI
CCHHHHCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEE
TRDQPELHIPTVAREVFDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGT
ECCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCC
STVTRLELIEALSLSHGETGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSY
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
LQQARALGDRLIVAVNTDASVKRLKGEGRPVNTEDRRMTVLAALASVDWVVPFTEDTPQR
HHHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCHHH
IISRLLPDLLVKGGDYKVEDIAGGAEVMAAGGQVKVLGFEDGISTTAIIQNIMANQ
HHHHHHHHHHHCCCCEEEHHHCCCHHEEECCCEEEEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA