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The map label for this gene is cysH [H]

Identifier: 167625449

GI number: 167625449

Start: 4318765

End: 4319535

Strand: Reverse

Name: cysH [H]

Synonym: Shal_3541

Alternate gene names: 167625449

Gene position: 4319535-4318765 (Counterclockwise)

Preceding gene: 167625450

Following gene: 167625448

Centisome position: 82.64

GC content: 47.86

Gene sequence:

>771_bases
ATGGTTGATACTCATAATATTGCTGATCCGGTGATTACATCAACTGCGCTCAAGGCGTTATTGAGCGCGCCTAAAGTAGA
GCAGCAAGCAGAATTAAAGCGAGTCAATGAGTTTTTGGCTCAGCTGACACCGATTGAGCGGGTGCGCTGGGGATTAAATT
ACTTGCCGGGTAATCATGGGTTATCGTCAAGTTTTGGGATCCAAGCGGCGGTAATGCTGCATATGGTTAGCGCTGAAAAA
GCGGATATCCCAGTGCTGCTCACCGATACCGGTTACCTCTTTAGCGAAACCTATCAATTTATCGATGAGTTGACCGCAAG
GCTATCGCTTAATCTCAAGGTGTATGCATCGCCTTATAGCGCTGCGTGGCAAGAGGCGCGCTTTGGTAAGTTGTGGGAGC
AAGACCTAGACGGTTTGGATAAATATAACCAGATCAACAAAGTTGAGCCTATGCAACGAGCCTTGGCTGAGCAAGAGATT
GGCACTTGGTTTGCCGGGTTGAGGCGTTCTCAATCGAGTACTCGTGAGGCTCTGCCGATTTTGGCGATTCACGGCCAGCG
CTACAAAATCCTGCCAATTATCGATTGGAACAATAAACAGGTGCATGAGTATTTAACCGAGTTTGAACTGCCTTACCACC
CATTATGGGAGCAAGGTTATGTCTCTGTGGGCGACACCCATTCGAGTAAGCCGCTAGAGCTGGGAATGAGTGAAGAGGAG
ACGCGTTTTAATGGATTAAAGCGTGAATGTGGTCTGCACTACGATATCTAA

Upstream 100 bases:

>100_bases
GCACAATATGCTTCACAGCGCCAAGCGGGTGAAACGTTTGGTAACTACACCGTGCGTAGCGGCGTTGTTGCGGCTGTAAA
TGATGCGGCTAAGGACTTTC

Downstream 100 bases:

>100_bases
TCTTGCAGTTAGAATGGCTCAGGTTATCGAGTTGCTAAGTGGCACAATCGTACCACTTGCGGCGCATGATTATAATGTTG
CATGCAAAATGCCATTTTTT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVDTHNIADPVITSTALKALLSAPKVEQQAELKRVNEFLAQLTPIERVRWGLNYLPGNHGLSSSFGIQAAVMLHMVSAEK
ADIPVLLTDTGYLFSETYQFIDELTARLSLNLKVYASPYSAAWQEARFGKLWEQDLDGLDKYNQINKVEPMQRALAEQEI
GTWFAGLRRSQSSTREALPILAIHGQRYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEE
TRFNGLKRECGLHYDI

Sequences:

>Translated_256_residues
MVDTHNIADPVITSTALKALLSAPKVEQQAELKRVNEFLAQLTPIERVRWGLNYLPGNHGLSSSFGIQAAVMLHMVSAEK
ADIPVLLTDTGYLFSETYQFIDELTARLSLNLKVYASPYSAAWQEARFGKLWEQDLDGLDKYNQINKVEPMQRALAEQEI
GTWFAGLRRSQSSTREALPILAIHGQRYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEE
TRFNGLKRECGLHYDI
>Mature_256_residues
MVDTHNIADPVITSTALKALLSAPKVEQQAELKRVNEFLAQLTPIERVRWGLNYLPGNHGLSSSFGIQAAVMLHMVSAEK
ADIPVLLTDTGYLFSETYQFIDELTARLSLNLKVYASPYSAAWQEARFGKLWEQDLDGLDKYNQINKVEPMQRALAEQEI
GTWFAGLRRSQSSTREALPILAIHGQRYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEE
TRFNGLKRECGLHYDI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=242, Percent_Identity=60.3305785123967, Blast_Score=302, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6325425, Length=239, Percent_Identity=34.3096234309623, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29149; Mature: 29149

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTHNIADPVITSTALKALLSAPKVEQQAELKRVNEFLAQLTPIERVRWGLNYLPGNHG
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
LSSSFGIQAAVMLHMVSAEKADIPVLLTDTGYLFSETYQFIDELTARLSLNLKVYASPYS
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCHH
AAWQEARFGKLWEQDLDGLDKYNQINKVEPMQRALAEQEIGTWFAGLRRSQSSTREALPI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAIHGQRYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEE
EEECCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEECCCCHHH
TRFNGLKRECGLHYDI
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVDTHNIADPVITSTALKALLSAPKVEQQAELKRVNEFLAQLTPIERVRWGLNYLPGNHG
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
LSSSFGIQAAVMLHMVSAEKADIPVLLTDTGYLFSETYQFIDELTARLSLNLKVYASPYS
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCHH
AAWQEARFGKLWEQDLDGLDKYNQINKVEPMQRALAEQEIGTWFAGLRRSQSSTREALPI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAIHGQRYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEE
EEECCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEECCCCHHH
TRFNGLKRECGLHYDI
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]