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The map label for this gene is btuB [H]

Identifier: 167625198

GI number: 167625198

Start: 4005552

End: 4007405

Strand: Reverse

Name: btuB [H]

Synonym: Shal_3286

Alternate gene names: 167625198

Gene position: 4007405-4005552 (Counterclockwise)

Preceding gene: 167625199

Following gene: 167625197

Centisome position: 76.67

GC content: 45.47

Gene sequence:

>1854_bases
ATGGGAACACAACCATCAAAAATCGCACTGCTAGTTAGCAGTCTTATTAGCCTATCTGCATTGTCGGCGCAGGCCGCTGA
CGTCGATGTAAACTCAACTGCTATCGATGAAGCAATCACAGTAACAGGGTCTCGTTTCGATACAACAGCCGATCAGCAAC
TGACCGTTATCAATACCATTGAGCGTGAAGAGATTGTACGCCTTAACCCTAAATCTGTTGCTGACGTATTAGAGACACTC
CCGGGTGTAAGCGTATCGCGTAATGGTGGCGCGGGTCAGGCAACGTCAATTAGTGTTCGTGGTAGTAATTCAAACCATGT
ATTAGTGCTGGTCGATGGCGTTAAAGTCGGATCGGCGACCTTAGGGACGGTGAGCTTTAATACTTTGTCGCCAGAGAATA
TCGAGCGTATCGAAGTGCTTAAGGGCCCTAGAGCCTCGGTTTGGGGCAGCGATGCGATTGGCGGCGTTATTCAGATCTTT
ACCCGTCAACTCGAAGGCGGAGAGTGGTTTGCTGGTGCTGAATATGGCTCGAATGATTATATTCGTGGCTCGTTTGGCGC
GGGAGTGAATCATGGCAATGGTAGCACAACAATCGCGGTCAATCATGAGCAGTCCGAAGGCTATGATGTGTATAACGGCG
CCGAAGTGGAAAACGATGATGATGGTTATAAGCGTAACGCGCTTTCACTGAAAGGCTCGCAGCAGATTAACTCGAATTGG
CAAGCGATTTGGAATGGTCAGTACGATAAAGGTAATACTCAATACGATGATATATATGCATCGGGTTCGGCAGATGAGTC
TGACTACGAGAATTATTTATGGAACCTAGCGGGTCAGTACCACAATGACAAATTTACCAGCAAGTTAGCACTGAGTCAGT
CTCGAGATTATAACGAAAACTTCCGTGGCGATGATGCGAGTGTCGCGACAGCTGAGTTTGAAACTAAGCGTGACCAAGTC
AACTGGAGTAACCAGTATTTAGCTGCCGATGATTTAACTCTGACCGGTGGTATCGATTGGTCAAATGAGTCGGTTAGAGG
TGACTATGCTCAAGATCAGCGTGATATTTTTGGTGTCTATGCATTAGTACAAAAACAGTGGAGTAAGTTGCTGGCTGAAG
TTGCCGTACGTTACGATGATGTTGAAAACATCGACAGTGAAGTCTCCTATAACGCCAGCCTCGCCTATGAGTTTAATGAG
CATTGGCGTCTCGCCGCCGCAACGGGCACCGCCTTTAAGGCGCCAACCTTCAACGATCTATATTGGCCAGACTCAGGTAA
TCCAGATCTTATCTCTGAAACCGCCGAAAGTTATGAACTGACATTGCATTTTAACAGCCAAGATCTTCGTGCTTATATTA
GTGTGTTCCAAAACAGCATCGATAATCTTATCGCTTGGGCACCTACAGGCGAGAAGGATGATTACGGTTGGGATATCTGG
AAGCCAGCTAACATCAATAAAGCCGAGATTAATGGTGTTGAACTGTCGGCAAATTATCAATTACTAGGGCTAGAACATCA
GCTTAGTTATACCTATCTAGATGCAGAAAACAAGCATACAAGTGAGCAGTTAGTTGGTCGTAGTGAGAACGAATTCAACT
ATTTGCTGAGTTATACTTGGCAACAGTTCGACTTGCTAGCAAACTACCATTATCAAGGTAAGCGCTACGCCGGCGATGAG
CAGTTCTTAGATGCCTACCACAAGTTAGATTTAAGCATTGGTTACCAGCTAGACGATGCATGGAGCTTACGATTAAAGGC
AAATAACCTGTTTGATGAAGAGATTATCTCAGATCAAAACTACTTTAGCCCTGGAAGAGAGTTATTCTTCAGCGTCAGCT
ACCAAGCATTTTAA

Upstream 100 bases:

>100_bases
GCGGCAATTAGACGAACCAGCAAACGGATGGAGTTTTTCCATTTATTGGTCGTATCACCGTGACGTTTTTGCCCCGTTTT
CCTTTAGGAGTCAAAGGTAA

Downstream 100 bases:

>100_bases
TTATCGCTGACGTATAGCGATTGCAAAAAAGCCAGACATTGTGTCTGGCTTTTTTTATGTTATAAATTGGCAAACTCCTA
AGTAGGCATGCCAATGAAAC

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MGTQPSKIALLVSSLISLSALSAQAADVDVNSTAIDEAITVTGSRFDTTADQQLTVINTIEREEIVRLNPKSVADVLETL
PGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSATLGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIF
TRQLEGGEWFAGAEYGSNDYIRGSFGAGVNHGNGSTTIAVNHEQSEGYDVYNGAEVENDDDGYKRNALSLKGSQQINSNW
QAIWNGQYDKGNTQYDDIYASGSADESDYENYLWNLAGQYHNDKFTSKLALSQSRDYNENFRGDDASVATAEFETKRDQV
NWSNQYLAADDLTLTGGIDWSNESVRGDYAQDQRDIFGVYALVQKQWSKLLAEVAVRYDDVENIDSEVSYNASLAYEFNE
HWRLAAATGTAFKAPTFNDLYWPDSGNPDLISETAESYELTLHFNSQDLRAYISVFQNSIDNLIAWAPTGEKDDYGWDIW
KPANINKAEINGVELSANYQLLGLEHQLSYTYLDAENKHTSEQLVGRSENEFNYLLSYTWQQFDLLANYHYQGKRYAGDE
QFLDAYHKLDLSIGYQLDDAWSLRLKANNLFDEEIISDQNYFSPGRELFFSVSYQAF

Sequences:

>Translated_617_residues
MGTQPSKIALLVSSLISLSALSAQAADVDVNSTAIDEAITVTGSRFDTTADQQLTVINTIEREEIVRLNPKSVADVLETL
PGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSATLGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIF
TRQLEGGEWFAGAEYGSNDYIRGSFGAGVNHGNGSTTIAVNHEQSEGYDVYNGAEVENDDDGYKRNALSLKGSQQINSNW
QAIWNGQYDKGNTQYDDIYASGSADESDYENYLWNLAGQYHNDKFTSKLALSQSRDYNENFRGDDASVATAEFETKRDQV
NWSNQYLAADDLTLTGGIDWSNESVRGDYAQDQRDIFGVYALVQKQWSKLLAEVAVRYDDVENIDSEVSYNASLAYEFNE
HWRLAAATGTAFKAPTFNDLYWPDSGNPDLISETAESYELTLHFNSQDLRAYISVFQNSIDNLIAWAPTGEKDDYGWDIW
KPANINKAEINGVELSANYQLLGLEHQLSYTYLDAENKHTSEQLVGRSENEFNYLLSYTWQQFDLLANYHYQGKRYAGDE
QFLDAYHKLDLSIGYQLDDAWSLRLKANNLFDEEIISDQNYFSPGRELFFSVSYQAF
>Mature_616_residues
GTQPSKIALLVSSLISLSALSAQAADVDVNSTAIDEAITVTGSRFDTTADQQLTVINTIEREEIVRLNPKSVADVLETLP
GVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSATLGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIFT
RQLEGGEWFAGAEYGSNDYIRGSFGAGVNHGNGSTTIAVNHEQSEGYDVYNGAEVENDDDGYKRNALSLKGSQQINSNWQ
AIWNGQYDKGNTQYDDIYASGSADESDYENYLWNLAGQYHNDKFTSKLALSQSRDYNENFRGDDASVATAEFETKRDQVN
WSNQYLAADDLTLTGGIDWSNESVRGDYAQDQRDIFGVYALVQKQWSKLLAEVAVRYDDVENIDSEVSYNASLAYEFNEH
WRLAAATGTAFKAPTFNDLYWPDSGNPDLISETAESYELTLHFNSQDLRAYISVFQNSIDNLIAWAPTGEKDDYGWDIWK
PANINKAEINGVELSANYQLLGLEHQLSYTYLDAENKHTSEQLVGRSENEFNYLLSYTWQQFDLLANYHYQGKRYAGDEQ
FLDAYHKLDLSIGYQLDDAWSLRLKANNLFDEEIISDQNYFSPGRELFFSVSYQAF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=631, Percent_Identity=30.9033280507132, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788478, Length=705, Percent_Identity=24.2553191489362, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1786798, Length=759, Percent_Identity=23.3201581027668, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68640; Mature: 68509

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQPSKIALLVSSLISLSALSAQAADVDVNSTAIDEAITVTGSRFDTTADQQLTVINTI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCEEEEECCCCCCCCCCEEEEEECC
EREEIVRLNPKSVADVLETLPGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSAT
CCCCEEEECHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCEECCEE
LGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIFTRQLEGGEWFAGAEYGSNDY
EEEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCE
IRGSFGAGVNHGNGSTTIAVNHEQSEGYDVYNGAEVENDDDGYKRNALSLKGSQQINSNW
EEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEECEEEECCCCCCCCCC
QAIWNGQYDKGNTQYDDIYASGSADESDYENYLWNLAGQYHNDKFTSKLALSQSRDYNEN
EEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
FRGDDASVATAEFETKRDQVNWSNQYLAADDLTLTGGIDWSNESVRGDYAQDQRDIFGVY
CCCCCCCEEEEEHHCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ALVQKQWSKLLAEVAVRYDDVENIDSEVSYNASLAYEFNEHWRLAAATGTAFKAPTFNDL
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEECCCEEEEEECCCEECCCCCCCC
YWPDSGNPDLISETAESYELTLHFNSQDLRAYISVFQNSIDNLIAWAPTGEKDDYGWDIW
CCCCCCCCCHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
KPANINKAEINGVELSANYQLLGLEHQLSYTYLDAENKHTSEQLVGRSENEFNYLLSYTW
CCCCCCCEECCCEEEECCEEEEEEEEEEEEEEECCCCCCCHHHHHCCCCCCCEEEEEHHH
QQFDLLANYHYQGKRYAGDEQFLDAYHKLDLSIGYQLDDAWSLRLKANNLFDEEIISDQN
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECEEECCCEEEEEEECCCCHHHHHCCCC
YFSPGRELFFSVSYQAF
CCCCCCEEEEEEEEECC
>Mature Secondary Structure 
GTQPSKIALLVSSLISLSALSAQAADVDVNSTAIDEAITVTGSRFDTTADQQLTVINTI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCEEEEECCCCCCCCCCEEEEEECC
EREEIVRLNPKSVADVLETLPGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSAT
CCCCEEEECHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCEECCEE
LGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIFTRQLEGGEWFAGAEYGSNDY
EEEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCE
IRGSFGAGVNHGNGSTTIAVNHEQSEGYDVYNGAEVENDDDGYKRNALSLKGSQQINSNW
EEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEECEEEECCCCCCCCCC
QAIWNGQYDKGNTQYDDIYASGSADESDYENYLWNLAGQYHNDKFTSKLALSQSRDYNEN
EEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
FRGDDASVATAEFETKRDQVNWSNQYLAADDLTLTGGIDWSNESVRGDYAQDQRDIFGVY
CCCCCCCEEEEEHHCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ALVQKQWSKLLAEVAVRYDDVENIDSEVSYNASLAYEFNEHWRLAAATGTAFKAPTFNDL
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEECCCEEEEEECCCEECCCCCCCC
YWPDSGNPDLISETAESYELTLHFNSQDLRAYISVFQNSIDNLIAWAPTGEKDDYGWDIW
CCCCCCCCCHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
KPANINKAEINGVELSANYQLLGLEHQLSYTYLDAENKHTSEQLVGRSENEFNYLLSYTW
CCCCCCCEECCCEEEECCEEEEEEEEEEEEEEECCCCCCCHHHHHCCCCCCCEEEEEHHH
QQFDLLANYHYQGKRYAGDEQFLDAYHKLDLSIGYQLDDAWSLRLKANNLFDEEIISDQN
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECEEECCCEEEEEEECCCCHHHHHCCCC
YFSPGRELFFSVSYQAF
CCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA