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The map label for this gene is rsuA [H]

Identifier: 167625168

GI number: 167625168

Start: 3971649

End: 3972341

Strand: Reverse

Name: rsuA [H]

Synonym: Shal_3256

Alternate gene names: 167625168

Gene position: 3972341-3971649 (Counterclockwise)

Preceding gene: 167625174

Following gene: 167625163

Centisome position: 76.0

GC content: 45.89

Gene sequence:

>693_bases
GTGCGTTTAGATAAATTTATTTGTGAGTCGACCTCACACTCTCGTGTTTCTGCTAAAAAAGCCCTACATCGTGGTGATGT
GACTTGTAATGGTGAAGTGGTTAAAAACTCGGGCTTTAAAGTGACCGATGAACATGAAATCCGTCTTGACGGTGAAGTGC
TAACCATCATTGGCCCGCGCTTTATCATGCTTAATAAACCTGTCGATACCATCTGCTCAACCATAGATGAAGAGTACCCA
TCGGTGATCAGCTTGCTAGACGTTATCCGCCCTGAAGACTTGCACATTGCTGGCCGTCTTGATGCCGATACTACCGGACT
TGTGTTAATCACGACTGACGGTCAGTGGTCGCATAAGATCACTTCGCCAAAGAAAGAGTGTGGTAAGCGCTATTTGCTAG
AGACTGCTGAATCGCTATCTGATGATTTAGTGCAGCAGTTTGTTGATGGTCTGCAGCTTAATAATGAAGATGGCCTGACT
AAACCGGCTGAACTTGAGATTTTAGCTCCGAACCAAGCGCGTTTGACGATTACCGAAGGTAAATATCATCAGGTTAAGCG
TATGCTGGCAGCGGTAGGTAACAAGGTGACTAAGCTACACCGTGAGAGCGTTGGCGCGATTGAACTCGACCCAAATCTGG
AGTTGGGTCAATGGCGTTACCTTACTGCTGCTGAGATAAAGTCAGTTAAGTAA

Upstream 100 bases:

>100_bases
ATACGCTTTTAGCCGCTGTTGATTCAAGCAAAACTTTGCCCTTTGACGGCGCTGGCCTCATAATGTCGGCCATTATGGTT
ATATAAGCTGAGTTCTGCCT

Downstream 100 bases:

>100_bases
GCAGTCCAGCTTTAACTAAACAACTTTGAGCTCGGTGAATGGCTGGTGGTTAAATTTTACTGCTGCTGAAATAAAGTCAG
TTAAGTAAGCAGTCCAGCTT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRLDKFICESTSHSRVSAKKALHRGDVTCNGEVVKNSGFKVTDEHEIRLDGEVLTIIGPRFIMLNKPVDTICSTIDEEYP
SVISLLDVIRPEDLHIAGRLDADTTGLVLITTDGQWSHKITSPKKECGKRYLLETAESLSDDLVQQFVDGLQLNNEDGLT
KPAELEILAPNQARLTITEGKYHQVKRMLAAVGNKVTKLHRESVGAIELDPNLELGQWRYLTAAEIKSVK

Sequences:

>Translated_230_residues
MRLDKFICESTSHSRVSAKKALHRGDVTCNGEVVKNSGFKVTDEHEIRLDGEVLTIIGPRFIMLNKPVDTICSTIDEEYP
SVISLLDVIRPEDLHIAGRLDADTTGLVLITTDGQWSHKITSPKKECGKRYLLETAESLSDDLVQQFVDGLQLNNEDGLT
KPAELEILAPNQARLTITEGKYHQVKRMLAAVGNKVTKLHRESVGAIELDPNLELGQWRYLTAAEIKSVK
>Mature_230_residues
MRLDKFICESTSHSRVSAKKALHRGDVTCNGEVVKNSGFKVTDEHEIRLDGEVLTIIGPRFIMLNKPVDTICSTIDEEYP
SVISLLDVIRPEDLHIAGRLDADTTGLVLITTDGQWSHKITSPKKECGKRYLLETAESLSDDLVQQFVDGLQLNNEDGLT
KPAELEILAPNQARLTITEGKYHQVKRMLAAVGNKVTKLHRESVGAIELDPNLELGQWRYLTAAEIKSVK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=53.0434782608696, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=28.8065843621399, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1790453, Length=232, Percent_Identity=28.0172413793103, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=32.4175824175824, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25602; Mature: 25602

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFICESTSHSRVSAKKALHRGDVTCNGEVVKNSGFKVTDEHEIRLDGEVLTIIGPR
CCCHHHHHCCCCCHHHHHHHHHHCCCCEECCEEEECCCCEECCCCEEEECCEEEEEECCE
FIMLNKPVDTICSTIDEEYPSVISLLDVIRPEDLHIAGRLDADTTGLVLITTDGQWSHKI
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCC
TSPKKECGKRYLLETAESLSDDLVQQFVDGLQLNNEDGLTKPAELEILAPNQARLTITEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCEEEEEECC
KYHQVKRMLAAVGNKVTKLHRESVGAIELDPNLELGQWRYLTAAEIKSVK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHCCCC
>Mature Secondary Structure
MRLDKFICESTSHSRVSAKKALHRGDVTCNGEVVKNSGFKVTDEHEIRLDGEVLTIIGPR
CCCHHHHHCCCCCHHHHHHHHHHCCCCEECCEEEECCCCEECCCCEEEECCEEEEEECCE
FIMLNKPVDTICSTIDEEYPSVISLLDVIRPEDLHIAGRLDADTTGLVLITTDGQWSHKI
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCC
TSPKKECGKRYLLETAESLSDDLVQQFVDGLQLNNEDGLTKPAELEILAPNQARLTITEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCEEEEEECC
KYHQVKRMLAAVGNKVTKLHRESVGAIELDPNLELGQWRYLTAAEIKSVK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]