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The map label for this gene is ubiF [H]

Identifier: 167625132

GI number: 167625132

Start: 3925399

End: 3926598

Strand: Reverse

Name: ubiF [H]

Synonym: Shal_3220

Alternate gene names: 167625132

Gene position: 3926598-3925399 (Counterclockwise)

Preceding gene: 167625139

Following gene: 167625131

Centisome position: 75.12

GC content: 47.0

Gene sequence:

>1200_bases
GTGGAACAGACGAGTACTAAGATCAATCTATATGATGCGGTTGTTGTGGGTGGTGGCATGGTTGGCGCCGCAACAGCAAT
TGGTCTTGGCCAACTAGGATTGAAGGTTGCGATAGTCGAAGCGTTTGCTCCCAAAGCATATGACAGTGAACAGCCATTGG
ATCTACGCGTCTCTGCTATCAGCGCTGCATCCGAAGCGCTGCTTGAGCGCTTAGGTGCATTAGCGGGGTTAGATGGCATG
CGCAAGGTTGCCTATAAAGGCCTCGAAACCTGGGAGATGGATGGCTGTATCACCCGTTTTCATAGCGATCAAATCGGCTC
TTCTCATTTAGGCCATATTCTTGAGAACCGTTTAATCCAACTTTCTCTTTGGCAACGCATGGAGCAGCTAGATAATATCA
CGCTGTTTTGTCCAACTAAGATAGCTAAACTGTCTCGCAGCTCTACTCAAGAACAGATCACAGTCGAACTCGATAGTGGT
GAGTTATTTATGACGCGCTTGCTCGTCGGTGCAGACGGGGCCAATTCCATGGTACGGCAGTGGGCCAATATCGGTATCAC
TGGTTGGGATTACGGGCAGTCGGCCATGTTGATCAACATCGAAACCGCCAGTGAAGAGCAAGATGTCACCTGGCAGCAAT
TTACGCCAAGTGGCCCGCGTTCGTTATTACCGTTGCCCGGTAATCATGCTTCATTAGTCTGGTATGACGACGCAAATAAG
ATAGCTCAGCTTTCTCAACTCAATAATCTAGCCTTGAAAGCGCAAATTGATCAGTACTTCCCTGAGCGGTTAGAACGCGA
TTTTCAGGTTATTAATAAGGCGAGTTTTAAACTGACCCGTCGCCATGCCCAAGTTTACTACCGTGATAACCTAGTGATCC
TAGGTGATGCTGCACACACCATTAATCCGTTAGCAGGGCAGGGCGTGAACTTAGGCTTTAAAGATGTCGATGCCTTAGTG
AATACCATTGCGAGTCATATTGATGATGAAAAAGGCTGGTGGCACAGTCAGGTGCTAGAAAAATACCAAAAATGCCGTTA
TCGAGATAATCAGTTGATGATGTCGACCATGGATCTGTTCTATGCAAGCTTTAGTAACGACATCTTACCGCTAAAGATAC
TGCGTAATGGCGTGCTAAAGCTTGCCAATATCGACGGACCAATTAAGAAGCAGGTACTTAAGTACGCCATGGGTTTTTAA

Upstream 100 bases:

>100_bases
CAAATAGTGCAATCTTTCTTATATAAAGCTCAATATTGATACCCTTTAGGTGTAAACTCGGTAGCGAAAAATGAGTTCAG
TTGTCAGGAGAGATAAAGCT

Downstream 100 bases:

>100_bases
TAAAGCTAAGTTGATGTCATGACTAAGTTGATGTCAAAACTTGGCTGATACCATCAGCATAAGATGAAAAACCGAATATT
ATGTATTCGGTTTTTTTTGT

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MEQTSTKINLYDAVVVGGGMVGAATAIGLGQLGLKVAIVEAFAPKAYDSEQPLDLRVSAISAASEALLERLGALAGLDGM
RKVAYKGLETWEMDGCITRFHSDQIGSSHLGHILENRLIQLSLWQRMEQLDNITLFCPTKIAKLSRSSTQEQITVELDSG
ELFMTRLLVGADGANSMVRQWANIGITGWDYGQSAMLINIETASEEQDVTWQQFTPSGPRSLLPLPGNHASLVWYDDANK
IAQLSQLNNLALKAQIDQYFPERLERDFQVINKASFKLTRRHAQVYYRDNLVILGDAAHTINPLAGQGVNLGFKDVDALV
NTIASHIDDEKGWWHSQVLEKYQKCRYRDNQLMMSTMDLFYASFSNDILPLKILRNGVLKLANIDGPIKKQVLKYAMGF

Sequences:

>Translated_399_residues
MEQTSTKINLYDAVVVGGGMVGAATAIGLGQLGLKVAIVEAFAPKAYDSEQPLDLRVSAISAASEALLERLGALAGLDGM
RKVAYKGLETWEMDGCITRFHSDQIGSSHLGHILENRLIQLSLWQRMEQLDNITLFCPTKIAKLSRSSTQEQITVELDSG
ELFMTRLLVGADGANSMVRQWANIGITGWDYGQSAMLINIETASEEQDVTWQQFTPSGPRSLLPLPGNHASLVWYDDANK
IAQLSQLNNLALKAQIDQYFPERLERDFQVINKASFKLTRRHAQVYYRDNLVILGDAAHTINPLAGQGVNLGFKDVDALV
NTIASHIDDEKGWWHSQVLEKYQKCRYRDNQLMMSTMDLFYASFSNDILPLKILRNGVLKLANIDGPIKKQVLKYAMGF
>Mature_399_residues
MEQTSTKINLYDAVVVGGGMVGAATAIGLGQLGLKVAIVEAFAPKAYDSEQPLDLRVSAISAASEALLERLGALAGLDGM
RKVAYKGLETWEMDGCITRFHSDQIGSSHLGHILENRLIQLSLWQRMEQLDNITLFCPTKIAKLSRSSTQEQITVELDSG
ELFMTRLLVGADGANSMVRQWANIGITGWDYGQSAMLINIETASEEQDVTWQQFTPSGPRSLLPLPGNHASLVWYDDANK
IAQLSQLNNLALKAQIDQYFPERLERDFQVINKASFKLTRRHAQVYYRDNLVILGDAAHTINPLAGQGVNLGFKDVDALV
NTIASHIDDEKGWWHSQVLEKYQKCRYRDNQLMMSTMDLFYASFSNDILPLKILRNGVLKLANIDGPIKKQVLKYAMGF

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=443, Percent_Identity=30.9255079006772, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI32967307, Length=334, Percent_Identity=30.5389221556886, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=45.1948051948052, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1789273, Length=391, Percent_Identity=36.8286445012788, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789274, Length=391, Percent_Identity=27.1099744245524, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25146631, Length=438, Percent_Identity=29.6803652968037, Blast_Score=157, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321694, Length=458, Percent_Identity=29.0393013100437, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI19920760, Length=436, Percent_Identity=28.8990825688073, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 44380; Mature: 44380

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTSTKINLYDAVVVGGGMVGAATAIGLGQLGLKVAIVEAFAPKAYDSEQPLDLRVSAI
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCCCCEEEHHHH
SAASEALLERLGALAGLDGMRKVAYKGLETWEMDGCITRFHSDQIGSSHLGHILENRLIQ
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEECCHHHHHHCCCCCCHHHHHHHHHHHHHH
LSLWQRMEQLDNITLFCPTKIAKLSRSSTQEQITVELDSGELFMTRLLVGADGANSMVRQ
HHHHHHHHHHCCEEEECCHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHCCCCHHHHHHH
WANIGITGWDYGQSAMLINIETASEEQDVTWQQFTPSGPRSLLPLPGNHASLVWYDDANK
HHHCCCCEECCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEECCHHH
IAQLSQLNNLALKAQIDQYFPERLERDFQVINKASFKLTRRHAQVYYRDNLVILGDAAHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCHHH
INPLAGQGVNLGFKDVDALVNTIASHIDDEKGWWHSQVLEKYQKCRYRDNQLMMSTMDLF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
YASFSNDILPLKILRNGVLKLANIDGPIKKQVLKYAMGF
HHHHCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEQTSTKINLYDAVVVGGGMVGAATAIGLGQLGLKVAIVEAFAPKAYDSEQPLDLRVSAI
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCCCCEEEHHHH
SAASEALLERLGALAGLDGMRKVAYKGLETWEMDGCITRFHSDQIGSSHLGHILENRLIQ
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEECCHHHHHHCCCCCCHHHHHHHHHHHHHH
LSLWQRMEQLDNITLFCPTKIAKLSRSSTQEQITVELDSGELFMTRLLVGADGANSMVRQ
HHHHHHHHHHCCEEEECCHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHCCCCHHHHHHH
WANIGITGWDYGQSAMLINIETASEEQDVTWQQFTPSGPRSLLPLPGNHASLVWYDDANK
HHHCCCCEECCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEECCHHH
IAQLSQLNNLALKAQIDQYFPERLERDFQVINKASFKLTRRHAQVYYRDNLVILGDAAHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCHHH
INPLAGQGVNLGFKDVDALVNTIASHIDDEKGWWHSQVLEKYQKCRYRDNQLMMSTMDLF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
YASFSNDILPLKILRNGVLKLANIDGPIKKQVLKYAMGF
HHHHCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]