Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 167625123

GI number: 167625123

Start: 3915086

End: 3916171

Strand: Reverse

Name: prfA

Synonym: Shal_3211

Alternate gene names: 167625123

Gene position: 3916171-3915086 (Counterclockwise)

Preceding gene: 167625124

Following gene: 167625122

Centisome position: 74.92

GC content: 47.33

Gene sequence:

>1086_bases
ATGAAGGACAGTGTGATTCGTAAGCTTGAAGGCTTACTTGAACGTAATGAAGAAGTGTTGGCGCTACTGAGCGATGCAAG
CATTATTGCCGATCAGGAGCGTTTCCGCGCCTTGTCTAAAGAATATTCTCAACTAGAAGATGTAGTGAGAACATTTAAGT
CCTATCAGCAAGCCGAAGAAGACTTTGAGTCGGCCAAAGAGATGATGGAAGAGGACGATCCTGAGCTAAAAGAGATGGCT
CAGGAAGAATATAAAGCGACAAAAGAAGCGATAGCTACGCTTGAAGCTGAGCTGCAAATTCTACTGCTTCCAAAAGATCC
GAATGATGACAATAACTGTTTTATCGAAATTCGCGCGGGCGCGGGTGGAGATGAAGCGGCTATTTTTGCGGGCGACCTGT
TCCGCATGTACAGCAGATACGCTGAAAGCAAGCGCTGGCAGATTGAAGTTATGAACACCAACGAAGGTGAGCATGGCGGC
TTTAAAGAAGTGATTGCGAAAATCAGTGGCGAAGGCGTTTACGGTAAACTGAAATTTGAATCAGGCGGACACCGTGTACA
ACGTGTTCCTGAGACCGAGTCACAAGGCCGAGTACATACTTCTGCTTGTACTGTGATTGTATTACCAGAAGTGCCTGAAG
CTGAAGCCATTGAAATTAACAAGGCTGACTTGAAGGTCGATACCTTCCGCGCATCGGGTGCCGGTGGTCAGCACGTTAAC
AAAACCGATTCAGCTATCCGTATTACTCACATTCCAACTGGGATCGTAGTTGAGTGTCAGGATCAGCGTTCACAGCATAA
GAACCGTGCCCAAGCGATGAGCGTACTTGCTGCGCGTATTCAAGCGGTTGAAGACGAGAAGCGTCGTAGTGCAGAAGAAA
GCACACGTCGCAACCTAGTCAGTAGTGGTGATCGTTCTGAGCGTATCCGTACTTACAACTACCCACAAGGTCGTGTAAGT
GAGCACAGAATCAACTTAACGCTGTACCGCTTAAACGAGTTTATGGAAGGCGACATCGATTGTGTTGTTGAGCCGCTTAT
CCAAGAAAACCAAGCGGATATGCTTGCAGCACTGGGTGAAGCTTAA

Upstream 100 bases:

>100_bases
ACACAAGCGCTTACCGCTGCGAGTCGACAAGGGGACCTGAATTCTCTTGGACAATTAAGAGCTGTGCTTGGACTAGATAA
AGACTAAGGTTAGCGTTTTA

Downstream 100 bases:

>100_bases
TTCTTCACTAATGTGATTATTGAGCTACAGATGAATAAGGATATTCCTTGCAATTAACTATCTCAGGGGCGCTTAAGTGG
GCCTCTTCTCAGCTTGAAGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKDSVIRKLEGLLERNEEVLALLSDASIIADQERFRALSKEYSQLEDVVRTFKSYQQAEEDFESAKEMMEEDDPELKEMA
QEEYKATKEAIATLEAELQILLLPKDPNDDNNCFIEIRAGAGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGG
FKEVIAKISGEGVYGKLKFESGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLVSSGDRSERIRTYNYPQGRVS
EHRINLTLYRLNEFMEGDIDCVVEPLIQENQADMLAALGEA

Sequences:

>Translated_361_residues
MKDSVIRKLEGLLERNEEVLALLSDASIIADQERFRALSKEYSQLEDVVRTFKSYQQAEEDFESAKEMMEEDDPELKEMA
QEEYKATKEAIATLEAELQILLLPKDPNDDNNCFIEIRAGAGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGG
FKEVIAKISGEGVYGKLKFESGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLVSSGDRSERIRTYNYPQGRVS
EHRINLTLYRLNEFMEGDIDCVVEPLIQENQADMLAALGEA
>Mature_361_residues
MKDSVIRKLEGLLERNEEVLALLSDASIIADQERFRALSKEYSQLEDVVRTFKSYQQAEEDFESAKEMMEEDDPELKEMA
QEEYKATKEAIATLEAELQILLLPKDPNDDNNCFIEIRAGAGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGG
FKEVIAKISGEGVYGKLKFESGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLVSSGDRSERIRTYNYPQGRVS
EHRINLTLYRLNEFMEGDIDCVVEPLIQENQADMLAALGEA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=43.046357615894, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=41.5697674418605, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=42.0289855072464, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=70.2777777777778, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=40.7738095238095, Blast_Score=230, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=291, Percent_Identity=36.426116838488, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=41.7721518987342, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=39.7553516819572, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SHEHH (B0TR27)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675417.1
- ProteinModelPortal:   B0TR27
- SMR:   B0TR27
- GeneID:   5905573
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3211
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- ProtClustDB:   PRK00591
- BioCyc:   SHAL458817:SHAL_3211-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40600; Mature: 40600

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSVIRKLEGLLERNEEVLALLSDASIIADQERFRALSKEYSQLEDVVRTFKSYQQAEE
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFESAKEMMEEDDPELKEMAQEEYKATKEAIATLEAELQILLLPKDPNDDNNCFIEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGGFKEVIAKISGEGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEECEEEEEEECCCCCCCEEEEECCCEEEEEEEECCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGDRSERIRTYNYPQGRVSEHRINLTLYRLNEFMEGDIDCVVEPLIQENQADMLAALGE
HCCCCCCCCEECCCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHCC
A
C
>Mature Secondary Structure
MKDSVIRKLEGLLERNEEVLALLSDASIIADQERFRALSKEYSQLEDVVRTFKSYQQAEE
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFESAKEMMEEDDPELKEMAQEEYKATKEAIATLEAELQILLLPKDPNDDNNCFIEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGGFKEVIAKISGEGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEECEEEEEEECCCCCCCEEEEECCCEEEEEEEECCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGDRSERIRTYNYPQGRVSEHRINLTLYRLNEFMEGDIDCVVEPLIQENQADMLAALGE
HCCCCCCCCEECCCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA