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The map label for this gene is nqrF [H]

Identifier: 167625094

GI number: 167625094

Start: 3886662

End: 3887909

Strand: Reverse

Name: nqrF [H]

Synonym: Shal_3182

Alternate gene names: 167625094

Gene position: 3887909-3886662 (Counterclockwise)

Preceding gene: 167625095

Following gene: 167625093

Centisome position: 74.38

GC content: 44.39

Gene sequence:

>1248_bases
ATGGATATTCTTAAATCTACTCCGCTCGAGGTTTACCTCGGTGTGAGTATGTTTACTGCTATTGTCTTAGGTTTGGTGTT
AATTATTTTATTTGCCAAGTCAAAGTTAGTGAACAGTGGTGACATTACAATTGGTATCAATGATGACCCAAAGAAGGGCA
TCAAATTACCTGCAGGTGGTAAATTGTTGGGTGCACTTGCGGATAACGGTATCTTCGTTTCCAGTGCTTGTGGCGGTGGT
GGTACCTGTGGTCAGTGTAAAGTACACGTAAAATCGGGTGGTGGTGATATTTTGCCAACCGAGATGGACCATATCACTAA
AGGTGAGGCTCGTGAGGGCTGTCGTTTATCGTGTCAGGTGAACGTTAAAACCGATATGGAGATCGAGCTCGAAGAGGAGA
TCTTCGGTGTTAAAAAGTGGGAATGTACCGTTATCTCTAACGATAACAAGGCCACTTTCATCAAAGAGCTTAAGCTTCAA
ATCCCTGATGGTGACACGGTTCCTTTCCGAGCTGGTGGTTATATTCAGATTGAAGCGCCAGCCCACCATGTTAAATATGC
AGATTTTGATATTCCTGCAGAGTATCGTGGTGATTGGGAGCATTTTGGTTTCTTCAACTTAGAATCGAAAGTTGATGATG
AGACAATCCGTGCATACTCGATGGCGAACTATCCTGAAGAGGAAGGTATCATCATGTTGAACGTGCGTATTGCGACGCCG
CCTCCACGTAACCTGTCACTGCCTTGTGGTAAGATGTCTTCGTATATCTTTAGCCTCAAAGAGGGCGATAAGGTGACAAT
TTCAGGTCCATTTGGTGAGTTCTTCGCTAAAGAAACTGATAATGAGATGGTTTTTGTCGGTGGTGGTGCAGGTATGGCGC
CGATGCGCTCGCATATCTTCGATCAGCTTAAGCGTCTTCATACTAAGCGTAAGATGAGCTTCTGGTACGGGGCACGTTCT
AAGCGTGAAATGTTCTATGTTGAAGATTTCGATGGCCTAGCGGCAGATAACGATAACTTCCAATGGCACGTTGCTCTTTC
AGACCCTCAACCCGAGGATAACTGGGATGGTAAGACAGGGTTCATTCATAACGTTCTTTATGAGAGCTATCTCAAAGATC
ATGAAGCGCCAGAAGATTGTGAGTACTACATGTGTGGTCCACCAATGATGAACGCTGCTGTAATTGCTATGCTTAAAGAT
CTTGGTGTCGAAGATGAAAATATCCTATTGGATGACTTCGGTGGCTAA

Upstream 100 bases:

>100_bases
TACTGCGGGTCTAATGGCGCTTGGATTTATGTCGTTTTCTGGTGTGTCCCTATAACAGGGAGCCACTGGGTTCTTATTTC
GGACTTTTAAGGATAAGTTA

Downstream 100 bases:

>100_bases
CCGCTTCTTATAAAGTTACAATCCGCAAAAGTGTCGTCATCGAGTGTGGAAAGTGGAGGTTGATAAGCTCATGCCACTTT
CCTTTCTAAGCCCTCGATAG

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MDILKSTPLEVYLGVSMFTAIVLGLVLIILFAKSKLVNSGDITIGINDDPKKGIKLPAGGKLLGALADNGIFVSSACGGG
GTCGQCKVHVKSGGGDILPTEMDHITKGEAREGCRLSCQVNVKTDMEIELEEEIFGVKKWECTVISNDNKATFIKELKLQ
IPDGDTVPFRAGGYIQIEAPAHHVKYADFDIPAEYRGDWEHFGFFNLESKVDDETIRAYSMANYPEEEGIIMLNVRIATP
PPRNLSLPCGKMSSYIFSLKEGDKVTISGPFGEFFAKETDNEMVFVGGGAGMAPMRSHIFDQLKRLHTKRKMSFWYGARS
KREMFYVEDFDGLAADNDNFQWHVALSDPQPEDNWDGKTGFIHNVLYESYLKDHEAPEDCEYYMCGPPMMNAAVIAMLKD
LGVEDENILLDDFGG

Sequences:

>Translated_415_residues
MDILKSTPLEVYLGVSMFTAIVLGLVLIILFAKSKLVNSGDITIGINDDPKKGIKLPAGGKLLGALADNGIFVSSACGGG
GTCGQCKVHVKSGGGDILPTEMDHITKGEAREGCRLSCQVNVKTDMEIELEEEIFGVKKWECTVISNDNKATFIKELKLQ
IPDGDTVPFRAGGYIQIEAPAHHVKYADFDIPAEYRGDWEHFGFFNLESKVDDETIRAYSMANYPEEEGIIMLNVRIATP
PPRNLSLPCGKMSSYIFSLKEGDKVTISGPFGEFFAKETDNEMVFVGGGAGMAPMRSHIFDQLKRLHTKRKMSFWYGARS
KREMFYVEDFDGLAADNDNFQWHVALSDPQPEDNWDGKTGFIHNVLYESYLKDHEAPEDCEYYMCGPPMMNAAVIAMLKD
LGVEDENILLDDFGG
>Mature_415_residues
MDILKSTPLEVYLGVSMFTAIVLGLVLIILFAKSKLVNSGDITIGINDDPKKGIKLPAGGKLLGALADNGIFVSSACGGG
GTCGQCKVHVKSGGGDILPTEMDHITKGEAREGCRLSCQVNVKTDMEIELEEEIFGVKKWECTVISNDNKATFIKELKLQ
IPDGDTVPFRAGGYIQIEAPAHHVKYADFDIPAEYRGDWEHFGFFNLESKVDDETIRAYSMANYPEEEGIIMLNVRIATP
PPRNLSLPCGKMSSYIFSLKEGDKVTISGPFGEFFAKETDNEMVFVGGGAGMAPMRSHIFDQLKRLHTKRKMSFWYGARS
KREMFYVEDFDGLAADNDNFQWHVALSDPQPEDNWDGKTGFIHNVLYESYLKDHEAPEDCEYYMCGPPMMNAAVIAMLKD
LGVEDENILLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323552, Length=191, Percent_Identity=25.130890052356, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=159, Percent_Identity=28.3018867924528, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 45936; Mature: 45936

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILKSTPLEVYLGVSMFTAIVLGLVLIILFAKSKLVNSGDITIGINDDPKKGIKLPAGG
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEECCCCC
KLLGALADNGIFVSSACGGGGTCGQCKVHVKSGGGDILPTEMDHITKGEAREGCRLSCQV
CEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEE
NVKTDMEIELEEEIFGVKKWECTVISNDNKATFIKELKLQIPDGDTVPFRAGGYIQIEAP
EECCCCEEEEHHHHCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCEECCCEEEEECC
AHHVKYADFDIPAEYRGDWEHFGFFNLESKVDDETIRAYSMANYPEEEGIIMLNVRIATP
CCEEEEECCCCCHHHCCCHHHCCEEECCCCCCHHHHHEEEECCCCCCCCEEEEEEEECCC
PPRNLSLPCGKMSSYIFSLKEGDKVTISGPFGEFFAKETDNEMVFVGGGAGMAPMRSHIF
CCCCCCCCCCHHHHHEEEECCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHH
DQLKRLHTKRKMSFWYGARSKREMFYVEDFDGLAADNDNFQWHVALSDPQPEDNWDGKTG
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCH
FIHNVLYESYLKDHEAPEDCEYYMCGPPMMNAAVIAMLKDLGVEDENILLDDFGG
HHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MDILKSTPLEVYLGVSMFTAIVLGLVLIILFAKSKLVNSGDITIGINDDPKKGIKLPAGG
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEECCCCC
KLLGALADNGIFVSSACGGGGTCGQCKVHVKSGGGDILPTEMDHITKGEAREGCRLSCQV
CEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEE
NVKTDMEIELEEEIFGVKKWECTVISNDNKATFIKELKLQIPDGDTVPFRAGGYIQIEAP
EECCCCEEEEHHHHCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCEECCCEEEEECC
AHHVKYADFDIPAEYRGDWEHFGFFNLESKVDDETIRAYSMANYPEEEGIIMLNVRIATP
CCEEEEECCCCCHHHCCCHHHCCEEECCCCCCHHHHHEEEECCCCCCCCEEEEEEEECCC
PPRNLSLPCGKMSSYIFSLKEGDKVTISGPFGEFFAKETDNEMVFVGGGAGMAPMRSHIF
CCCCCCCCCCHHHHHEEEECCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHH
DQLKRLHTKRKMSFWYGARSKREMFYVEDFDGLAADNDNFQWHVALSDPQPEDNWDGKTG
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCH
FIHNVLYESYLKDHEAPEDCEYYMCGPPMMNAAVIAMLKDLGVEDENILLDDFGG
HHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA