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The map label for this gene is gadA [H]

Identifier: 167624956

GI number: 167624956

Start: 3712822

End: 3714216

Strand: Reverse

Name: gadA [H]

Synonym: Shal_3043

Alternate gene names: 167624956

Gene position: 3714216-3712822 (Counterclockwise)

Preceding gene: 167624958

Following gene: 167624955

Centisome position: 71.06

GC content: 46.45

Gene sequence:

>1395_bases
ATGCCTCTTCACTCAAAAGATACTGTGCGAGACGACCTCTTAGATGATATCTACTCATCGACAGATTTAGCTTTATCTAT
GCCTAAATATAAGATGCCGGAGCAGGAAAATAATTCTCGCCACGCATATCAAATCGTACACGACGAGCTGATGATGGACG
GAAACTCCCGTCAAAATCTTGCGACATTTTGCCAAACCTGGGTTGAGGATGAAGTTCATCAATTGATGGACGAATGTATC
GATAAGAATATGATCGATAAGGATGAATACCCTCAAACCGCAGAGCTTGAAGCCCGCTGTGTGCATATGCTTGCCGATCT
ATGGAACTCGCCGGATGCAGAAAACACATTAGGTTGCTCTACGACAGGCTCTAGTGAGGCTGCGATGTTAGGCGGTATGG
CTCTTAAGTGGGCTTGGCGCAAGAAAATGAAAGCGCTGGGTAAACCTACTGATAAACCCAATATGATTTGTGGACCGGTG
CAGGTTTGCTGGCATAAATTCGCGCGTTATTGGGACATAGAGTTACGTGAGATCCCGATGGAAGGCGATCGTTTAATCAT
GAATGCTGAAGAAGTGATTAAGCGATGTGACGAAAATACCATAGGCGTAGTGCCAACATTAGGCGTTACCTTTACCTGCC
AGTACGAGCCTGTAAAGGCTGTACATGATGCGCTCGATCAGCTGCAAAAAGATACCGGATTGGATATTCCAATGCATGTT
GATGCCGCAAGCGGTGGCTTTTTAGCGCCATTTTGCCAACCAGATCTAGAATGGGACTTCAAACTACCACGCGTTAAGTC
GATTAATGCGTCAGGACATAAATTTGGCTTGAGTCCACTCGGTGTAGGTTGGGTTATTTGGCGCGATGCTTCAGTTTTAG
ATGAAGACCTCATTTTTAACGTGAACTACTTAGGCGGTAATATGCCGACCTTTGCATTGAACTTCTCTCGCCCTGGTGGG
CAGATTGTCGCGCAGTATTATAATTTCTTACGTTTAGGTAAGGAAGGCTATCGTAAGATCCATCAGGCTTGCTACGACAC
GGCGCAATACCTGTCGAGCGAGATTGAAAAATTAGGCATGTTCGAGATTATCTATGATGGCCATGGCGGGATCCCTGCGA
TGAGTTGGTCCTTAAAAGAGGGCGTTGATCCTGGGTTCAACCTGTTTGATCTCTCGGATCGTATCCGCTCTCGAGGTTGG
CAGATTGCAGCCTATGCAATGCCGCCTAAAAGAGAGGACTTAGTCATTATGCGTATTTTGGTTCGCCATGGCTTTAGCCG
AGATCAGGCAGACCTCCTAGTGGCAGATTTGAAGCATTGTGTCGACTTCTTTGCCTCCCATCCGATTTCACACGGCAGTG
ATGCCAAAGAGTCATCCGGTTTCAATCATGGTTAA

Upstream 100 bases:

>100_bases
TGAGTATTGCAGGGAGTCTTACTATCTTCTATGTTGTTTACATCAGCTGACCATCGCCAGCTAGTAGGAAATTTTATTTA
ATTCATTGGAGTAAAATATA

Downstream 100 bases:

>100_bases
CTAGTCGAGAGGTCGTCTGCAAAGACGGCCTTTTTCTATCCAGTTTAATACCAATCGGTATAAAGGTGTGCTCGCTCAGC
GTGAGTTTAGTGCTTTTGAG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPLHSKDTVRDDLLDDIYSSTDLALSMPKYKMPEQENNSRHAYQIVHDELMMDGNSRQNLATFCQTWVEDEVHQLMDECI
DKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMICGPV
QVCWHKFARYWDIELREIPMEGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDQLQKDTGLDIPMHV
DAASGGFLAPFCQPDLEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASVLDEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGMFEIIYDGHGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVDFFASHPISHGSDAKESSGFNHG

Sequences:

>Translated_464_residues
MPLHSKDTVRDDLLDDIYSSTDLALSMPKYKMPEQENNSRHAYQIVHDELMMDGNSRQNLATFCQTWVEDEVHQLMDECI
DKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMICGPV
QVCWHKFARYWDIELREIPMEGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDQLQKDTGLDIPMHV
DAASGGFLAPFCQPDLEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASVLDEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGMFEIIYDGHGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVDFFASHPISHGSDAKESSGFNHG
>Mature_463_residues
PLHSKDTVRDDLLDDIYSSTDLALSMPKYKMPEQENNSRHAYQIVHDELMMDGNSRQNLATFCQTWVEDEVHQLMDECID
KNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMICGPVQ
VCWHKFARYWDIELREIPMEGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDQLQKDTGLDIPMHVD
AASGGFLAPFCQPDLEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASVLDEDLIFNVNYLGGNMPTFALNFSRPGGQ
IVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGMFEIIYDGHGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQ
IAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVDFFASHPISHGSDAKESSGFNHG

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=392, Percent_Identity=24.4897959183673, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787769, Length=459, Percent_Identity=56.8627450980392, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789934, Length=458, Percent_Identity=56.9868995633188, Blast_Score=565, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17557272, Length=375, Percent_Identity=25.3333333333333, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17543922, Length=331, Percent_Identity=23.8670694864048, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI25148342, Length=321, Percent_Identity=22.1183800623053, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323905, Length=457, Percent_Identity=40.0437636761488, Blast_Score=319, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6320500, Length=340, Percent_Identity=25.2941176470588, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355963, Length=425, Percent_Identity=25.1764705882353, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24654344, Length=425, Percent_Identity=25.1764705882353, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52394; Mature: 52263

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHSKDTVRDDLLDDIYSSTDLALSMPKYKMPEQENNSRHAYQIVHDELMMDGNSRQNL
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
ATFCQTWVEDEVHQLMDECIDKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMICGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDQLQKDTGLDIPMHV
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEE
DAASGGFLAPFCQPDLEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASVLDEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCCHHCCCCCEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FEIIYDGHGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEEECCCCCCCCCCCHHHCCCCCCCEECHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCVDFFASHPISHGSDAKESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
PLHSKDTVRDDLLDDIYSSTDLALSMPKYKMPEQENNSRHAYQIVHDELMMDGNSRQNL
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
ATFCQTWVEDEVHQLMDECIDKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMICGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDQLQKDTGLDIPMHV
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEE
DAASGGFLAPFCQPDLEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASVLDEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCCHHCCCCCEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FEIIYDGHGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEEECCCCCCCCCCCHHHCCCCCCCEECHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCVDFFASHPISHGSDAKESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]