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The map label for this gene is cpsA1 [H]

Identifier: 167624933

GI number: 167624933

Start: 3682322

End: 3683653

Strand: Reverse

Name: cpsA1 [H]

Synonym: Shal_3019

Alternate gene names: 167624933

Gene position: 3683653-3682322 (Counterclockwise)

Preceding gene: 167624935

Following gene: 167624929

Centisome position: 70.47

GC content: 48.65

Gene sequence:

>1332_bases
ATGAGATTACTAACAGCCTCTTTGCTTACACTACTTATTAGTTGTAGCTGGACCACTTGTTATGCCACAGGTTTAGCCGA
CTCTACGGCTAAGGCGATGCCTAACCTTGAAGCCTTATATTTGTACCTGCATCAAAACCCCGAACTCTCTTACCAAGAAA
AAGACAGTAGCGCTCGTATAGCGATAGAGTTAGCAAGTTTGGGTTTCCAAGTTACTGAAAATTTTGGTGGTTATGGCGTC
GTGGCCATATTAAGAAATGGTGAGGGGCCAACAGTGATGATAAGGGCTGACACCGACGGCCTGCCGATTACCGAGGAAAC
GGGTAAAACCTATGCCTCTAAAGTCACTACTTTGGATGCCAATAACAATGAGGTCGGGGTGATGCATGGTTGTGGTCATG
ACATACATATGACCAGTCTTGTGGGCACCGCTCAGCAACTTGTGGCCAATAAAGCGCATTGGCAAGGCACCTTGATGATG
GTGGCACAGCCTGCCGAGGAGGTGGGCGGCGGCGCAAAAGCCATGTTAAAGCAGGGATTGTTTGAGCAATTTCCCACACC
AGATTATGTGCTTGGGTTGCATGTTAGTGCATCGATACCAGCTGGTAAGGTGGGGATCGTTTCAGGCTACGCATTGGCGA
ACGTCGACTCTGTCGATATCACAGTTAAAGGCGAGGGCGGTCATGGCGCCTATCCGCACCAAACTAAAGATCCTGTGGTG
TTAGCCGCGAGAATCGTTGTAGCGTTGCAGACGATAGCGAGTCGAGAGGTGTCGCCGCTAGAACCTAATGTCATCACAGT
CGGTTCGATTCATGGTGGTTCAAAGCACAATATTATCTCTAATGAAGTGAAGTTACAGCTTACCCTGCGCTCCTATAATC
CCGATGTTCGCCTGCAACAAATTGCCGCCCTTAAGCGTTTAACAAAAGGGATTGCGATGAGTGCTGGGCTAGATGAAGCC
TTGTATCCTGAGGTGTATGTGCATCAAGGCGAGAGTATTCCATCGACTTATAATGATCCTGAATTAGCAGCGCGAGTGCA
GGCAAGTATCGAGTCTGAAATCGGCGCAGAAAACGTCATCGCTGCCTCGCCTGTGATGGCGGGAGAAGACTTTGGTTTAT
ATGGTTTAACAGATGACAAACGCCCTATCATGATGTTTTGGTTAGGGGCGGTAGAGCCGAGTCTCTATCAAACGAGCCTC
TCTTCCAGTAAGACACTACCGTCATTGCACTCCAGTAAATTTGCTCCCGATTACGCACCTACCATTAACACCGGTGTGAG
GGCGATGACCAAGAGTGCCATGGACTTGTTTGTTCACAAACATGACGACTGA

Upstream 100 bases:

>100_bases
GTTCACTGGTTTATATATGTTGACACTGTGTTAACTTGGTCTACTAAGGGATGGTAAATGCGTACACAATGGCGTAGCCG
AAAAATAAGAGAGATAAATC

Downstream 100 bases:

>100_bases
GTCATGCTTTGGAGCTAGATATTGCATCATTTGGACCTAGGTGTCGCATCAAAGGTTAGCTTGCAAGCTAAAAGCTAGCG
TATGCGCTGTAGCTTATTTT

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRLLTASLLTLLISCSWTTCYATGLADSTAKAMPNLEALYLYLHQNPELSYQEKDSSARIAIELASLGFQVTENFGGYGV
VAILRNGEGPTVMIRADTDGLPITEETGKTYASKVTTLDANNNEVGVMHGCGHDIHMTSLVGTAQQLVANKAHWQGTLMM
VAQPAEEVGGGAKAMLKQGLFEQFPTPDYVLGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHQTKDPVV
LAARIVVALQTIASREVSPLEPNVITVGSIHGGSKHNIISNEVKLQLTLRSYNPDVRLQQIAALKRLTKGIAMSAGLDEA
LYPEVYVHQGESIPSTYNDPELAARVQASIESEIGAENVIAASPVMAGEDFGLYGLTDDKRPIMMFWLGAVEPSLYQTSL
SSSKTLPSLHSSKFAPDYAPTINTGVRAMTKSAMDLFVHKHDD

Sequences:

>Translated_443_residues
MRLLTASLLTLLISCSWTTCYATGLADSTAKAMPNLEALYLYLHQNPELSYQEKDSSARIAIELASLGFQVTENFGGYGV
VAILRNGEGPTVMIRADTDGLPITEETGKTYASKVTTLDANNNEVGVMHGCGHDIHMTSLVGTAQQLVANKAHWQGTLMM
VAQPAEEVGGGAKAMLKQGLFEQFPTPDYVLGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHQTKDPVV
LAARIVVALQTIASREVSPLEPNVITVGSIHGGSKHNIISNEVKLQLTLRSYNPDVRLQQIAALKRLTKGIAMSAGLDEA
LYPEVYVHQGESIPSTYNDPELAARVQASIESEIGAENVIAASPVMAGEDFGLYGLTDDKRPIMMFWLGAVEPSLYQTSL
SSSKTLPSLHSSKFAPDYAPTINTGVRAMTKSAMDLFVHKHDD
>Mature_443_residues
MRLLTASLLTLLISCSWTTCYATGLADSTAKAMPNLEALYLYLHQNPELSYQEKDSSARIAIELASLGFQVTENFGGYGV
VAILRNGEGPTVMIRADTDGLPITEETGKTYASKVTTLDANNNEVGVMHGCGHDIHMTSLVGTAQQLVANKAHWQGTLMM
VAQPAEEVGGGAKAMLKQGLFEQFPTPDYVLGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHQTKDPVV
LAARIVVALQTIASREVSPLEPNVITVGSIHGGSKHNIISNEVKLQLTLRSYNPDVRLQQIAALKRLTKGIAMSAGLDEA
LYPEVYVHQGESIPSTYNDPELAARVQASIESEIGAENVIAASPVMAGEDFGLYGLTDDKRPIMMFWLGAVEPSLYQTSL
SSSKTLPSLHSSKFAPDYAPTINTGVRAMTKSAMDLFVHKHDD

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=246, Percent_Identity=28.8617886178862, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 47308; Mature: 47308

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLTASLLTLLISCSWTTCYATGLADSTAKAMPNLEALYLYLHQNPELSYQEKDSSARI
CCCHHHHHHHHHHHCCCHHEEEECCCCCHHHHCCCCEEEEEEEECCCCCCEECCCCCCEE
AIELASLGFQVTENFGGYGVVAILRNGEGPTVMIRADTDGLPITEETGKTYASKVTTLDA
EEEEECCCEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHCCCHHHHEEEEEEC
NNNEVGVMHGCGHDIHMTSLVGTAQQLVANKAHWQGTLMMVAQPAEEVGGGAKAMLKQGL
CCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHCCCHHHHHHHHH
FEQFPTPDYVLGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHQTKDPVV
HHHCCCCCEEEEEEEECCCCCCCEEEEECEEEECCCEEEEEEEECCCCCCCCCCCCCHHH
LAARIVVALQTIASREVSPLEPNVITVGSIHGGSKHNIISNEVKLQLTLRSYNPDVRLQQ
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEECCEEEEEEEEECCCCCCHHHH
IAALKRLTKGIAMSAGLDEALYPEVYVHQGESIPSTYNDPELAARVQASIESEIGAENVI
HHHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
AASPVMAGEDFGLYGLTDDKRPIMMFWLGAVEPSLYQTSLSSSKTLPSLHSSKFAPDYAP
ECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHHCCCCCCCCCC
TINTGVRAMTKSAMDLFVHKHDD
CCHHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure
MRLLTASLLTLLISCSWTTCYATGLADSTAKAMPNLEALYLYLHQNPELSYQEKDSSARI
CCCHHHHHHHHHHHCCCHHEEEECCCCCHHHHCCCCEEEEEEEECCCCCCEECCCCCCEE
AIELASLGFQVTENFGGYGVVAILRNGEGPTVMIRADTDGLPITEETGKTYASKVTTLDA
EEEEECCCEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHCCCHHHHEEEEEEC
NNNEVGVMHGCGHDIHMTSLVGTAQQLVANKAHWQGTLMMVAQPAEEVGGGAKAMLKQGL
CCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHCCCHHHHHHHHH
FEQFPTPDYVLGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHQTKDPVV
HHHCCCCCEEEEEEEECCCCCCCEEEEECEEEECCCEEEEEEEECCCCCCCCCCCCCHHH
LAARIVVALQTIASREVSPLEPNVITVGSIHGGSKHNIISNEVKLQLTLRSYNPDVRLQQ
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEECCEEEEEEEEECCCCCCHHHH
IAALKRLTKGIAMSAGLDEALYPEVYVHQGESIPSTYNDPELAARVQASIESEIGAENVI
HHHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
AASPVMAGEDFGLYGLTDDKRPIMMFWLGAVEPSLYQTSLSSSKTLPSLHSSKFAPDYAP
ECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHHCCCCCCCCCC
TINTGVRAMTKSAMDLFVHKHDD
CCHHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]