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The map label for this gene is truC [H]

Identifier: 167624902

GI number: 167624902

Start: 3633571

End: 3634410

Strand: Reverse

Name: truC [H]

Synonym: Shal_2988

Alternate gene names: 167624902

Gene position: 3634410-3633571 (Counterclockwise)

Preceding gene: 167624903

Following gene: 167624901

Centisome position: 69.53

GC content: 47.14

Gene sequence:

>840_bases
ATGAGTGATGAAACGAGAGAAGTAGCAGATAGCGAACTGCTTGATATTGAGGCTGAAGAGATCGCCCCTCATATTGAAGT
GTTATTCGAAGATGATGATGTGGTGGCGATCCACAAGCCTGCCGGTCTTTTGGTTCACCGTAGTTACCTTGCTCGTCGTG
AGCGCTTTTTTGCCATGCAGCTCACGCGCGATAAAGTCGGCTGTCATGTGTTTCCTGTACACAGGCTCGACAGACCAACA
TCGGGAATATTGTTATTCGCTAAAAGCAGTGAAATGGCCAACGCATTATGCGGGCAATTTGCCGATCATACAGTCGATAA
AGAGTACTTGGCCATTGTCCGTGGCAATATGCATGAGGCCGGTACGCTCGATTATGCATTAAAAGAGGAACTCGATGAGC
TAGGTGATAAAGATGTTGATCCGAATAAGGCTGCGCAAGATGCAGTGACAAGTTATCAGCCGTTGTTAAATAGTGAGATC
CCATTCTCTTCTGGCCGTTATGCCACGAGTCGTTACGCGCTAGTTCAGCTTTCCCCCCATACTGGGCGTAAGCACCAGCT
AAGACGTCATATGGCACACTTACGTCACCCGATCCTTGGAGATACAACCCATGGTGATGGAAAGCAAAATAAGTTTTTCC
GTTCTCACTTCGGGATTAATCGGTTATGGCTTATCGCAAAGAAGCTGAGCTTTAATCACCCTAGAACAGGGGAGAGGATC
GAGATTGAGACTGAACTAGAGACAGAATGGCTACAGCTATTTGAAGGGCTGGGCTGGCATGAAGACACTTTATCTGTCGA
CAAACAACCGCTTATCATCGCTAACGGGTGCAAAATCTAG

Upstream 100 bases:

>100_bases
TTGGGCAAGTCAGCCTGAGTTACAAAATTTAATTAGTATTTTAACTGATTTAGACACGCTATTGAGTCAGACAGAATAGC
GCTGTTAAGAGAGATTTGAA

Downstream 100 bases:

>100_bases
TTGATTTATCAGTTAGCTGTTATTTGTCGAACCAATGTGGATTGCTGCTTAAGACATTTATTTTGTTGTGCTTTTAGTTT
TTTCGCTAAGGCTTGGGGGG

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSDETREVADSELLDIEAEEIAPHIEVLFEDDDVVAIHKPAGLLVHRSYLARRERFFAMQLTRDKVGCHVFPVHRLDRPT
SGILLFAKSSEMANALCGQFADHTVDKEYLAIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVTSYQPLLNSEI
PFSSGRYATSRYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLSFNHPRTGERI
EIETELETEWLQLFEGLGWHEDTLSVDKQPLIIANGCKI

Sequences:

>Translated_279_residues
MSDETREVADSELLDIEAEEIAPHIEVLFEDDDVVAIHKPAGLLVHRSYLARRERFFAMQLTRDKVGCHVFPVHRLDRPT
SGILLFAKSSEMANALCGQFADHTVDKEYLAIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVTSYQPLLNSEI
PFSSGRYATSRYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLSFNHPRTGERI
EIETELETEWLQLFEGLGWHEDTLSVDKQPLIIANGCKI
>Mature_278_residues
SDETREVADSELLDIEAEEIAPHIEVLFEDDDVVAIHKPAGLLVHRSYLARRERFFAMQLTRDKVGCHVFPVHRLDRPTS
GILLFAKSSEMANALCGQFADHTVDKEYLAIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVTSYQPLLNSEIP
FSSGRYATSRYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLSFNHPRTGERIE
IETELETEWLQLFEGLGWHEDTLSVDKQPLIIANGCKI

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=192, Percent_Identity=31.25, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI17158025, Length=145, Percent_Identity=33.7931034482759, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221316663, Length=204, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=52.3404255319149, Blast_Score=257, Evalue=6e-70,
Organism=Escherichia coli, GI1787327, Length=256, Percent_Identity=30.46875, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788946, Length=244, Percent_Identity=25.8196721311475, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786244, Length=219, Percent_Identity=30.5936073059361, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17534965, Length=185, Percent_Identity=29.1891891891892, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320168, Length=185, Percent_Identity=32.4324324324324, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324506, Length=185, Percent_Identity=31.8918918918919, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321608, Length=207, Percent_Identity=28.5024154589372, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583449, Length=177, Percent_Identity=30.5084745762712, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583447, Length=177, Percent_Identity=30.5084745762712, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583445, Length=177, Percent_Identity=30.5084745762712, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921090, Length=181, Percent_Identity=29.8342541436464, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31740; Mature: 31609

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDETREVADSELLDIEAEEIAPHIEVLFEDDDVVAIHKPAGLLVHRSYLARRERFFAMQ
CCCCHHHHHCHHHHCCCHHHCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHEE
LTRDKVGCHVFPVHRLDRPTSGILLFAKSSEMANALCGQFADHTVDKEYLAIVRGNMHEA
ECCCCCCEEEEEHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
GTLDYALKEELDELGDKDVDPNKAAQDAVTSYQPLLNSEIPFSSGRYATSRYALVQLSPH
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECEEEEEEECCC
TGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLSFNHPRTGERI
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCEE
EIETELETEWLQLFEGLGWHEDTLSVDKQPLIIANGCKI
EEEECHHHHHHHHHHCCCCCCHHCCCCCCCEEEECCCCC
>Mature Secondary Structure 
SDETREVADSELLDIEAEEIAPHIEVLFEDDDVVAIHKPAGLLVHRSYLARRERFFAMQ
CCCHHHHHCHHHHCCCHHHCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHEE
LTRDKVGCHVFPVHRLDRPTSGILLFAKSSEMANALCGQFADHTVDKEYLAIVRGNMHEA
ECCCCCCEEEEEHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
GTLDYALKEELDELGDKDVDPNKAAQDAVTSYQPLLNSEIPFSSGRYATSRYALVQLSPH
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECEEEEEEECCC
TGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLSFNHPRTGERI
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCEE
EIETELETEWLQLFEGLGWHEDTLSVDKQPLIIANGCKI
EEEECHHHHHHHHHHCCCCCCHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]