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The map label for this gene is lcfB [H]

Identifier: 167624735

GI number: 167624735

Start: 3426005

End: 3427726

Strand: Reverse

Name: lcfB [H]

Synonym: Shal_2818

Alternate gene names: 167624735

Gene position: 3427726-3426005 (Counterclockwise)

Preceding gene: 167624736

Following gene: 167624734

Centisome position: 65.58

GC content: 49.42

Gene sequence:

>1722_bases
ATGGTAAGTCAGTTGACTCAAAAAAATGACGGCGCAAATTTGTGCCGTCATTTAGTTAGTGCCGCACAGACTATCCCCGA
TTCACTAGCGGTTGCAGTGCAGCAATCTAAGGGCAGAGCCGTAAGTGGTCTGCGTTATCAAGAGATGGATTTTCAAACCC
TCAACGCTAAGAGCGATGAGCTGGCTAGCGCGCTTATCAGCCATGGTTTAATGCCTGGCATGAAAGCGGTATTGATGGTC
ACTCCTAGCATCGATTTTTTCTGCTTAACCTTTGCCCTGTTTAAGGCCGGCATTATTCCTATCTTGGTCGACCCTGGCAT
GGGCGTTAAAAACCTTAAGCAGTGTTTTATTGAGGCTAAGCCGGACGCTTTTATCGGGATCCCCAAAGCCCATATCGCCA
GACGGCTTTTTGGCTGGGGCAAACCCACGGTTAAGCACTTAATCAATGTGGGGGGCAGCGCTTTCGCTCAGGCGCTGACG
GGGGCGACGAGCCTAGAGAGCCTATTGAAAGCGAACAGGTCACCAACTACAGTGCCATTTAATATGCAGTGGCTCGATGA
AGATGCCATGGCGGCAATCTTGTTTACCAGCGGCAGTACGGGCACGCCAAAGGGCGTGGTTTATTCCCATAAGATGTTCG
AGGCGCAAATTACCGCGCTTAAACATGATTACGCCATTAAACCAGGCGAACGTGACTTAGCCACCTTCCCGCTGTTTTCA
CTGTTTGGTCCCGCTTTAGGTATGGCATCTATCGTACCGGATATGGATGCCAGTAAACCCATTACCGCCAATCCTGATTT
TATCTTTGCTGCCATCGATAAATATCAGTGCAGCAATATGTTTGTAAACCCTGCGTTGATAGAGCGCCTTGGCCAAGCGG
GTGAAGCCAAACAACACAAACTGGCTAGCATTAAACGGGTGATCTCAGCTGGAGCGCCAGCGACCATCTCATCGATTAAA
CGCTTTAGTCAGATGCTAAATCAAGACGTTGAAGTGCTTAACTCTTACGGCGCGACCGAGTCTTTGCCTATTAGTAAAAT
TGGTAGCCAAGCCTTGTTTGACACGACGTTTGTGACCGATAACGGTGGCGGGATTTGTGTCGGTAAAGCCATTGATGGCG
TTGAAATTGCCATCATAGCTATCGACGAGCAGCCGATTATACATTGGCACGACAGCTTAGCCTTGCCTGTTGATGCAATA
GGCGAAATCGTCGTTAAAGGCCCTATGGTCAGTCGCAGTTATTATCAGCGTGAAAGCGCGACGCAGGTGGCTAAAATTCA
AGATGGCGCCAGTATTCGCCACCGTATGGGTGACTTGGGCTACTTGGATAGTGATGGTTTGCTGTGGATGTGTGGCCGCA
AGGCTCATCGCGTCGATGCCGATGCTCTGCCTGCAACGAATAATCAGGCTCAACGTTATTACTCTATTCCTTGTGAGCGT
ATTTTTAATACCCATAGTGATGTTAAACGCAGCGCGTTAGTGGGCGTAAAGGTGGCGGGCAGGGTGACACCTCTTGTCTG
TATCGAGCTCAAGCAGTCGGTCGCCTGTTCGTTATCGGGTGTGCTTTATAGTGAGCTTCGCGCGATTGCCGAGCAGCATG
CGCAGACTCAAGGTATAGATCGCTTCTTCATTCACCCCGACTTTCCGGTGGATATTCGCCATAATGCCAAAATTTTCCGT
GAACAATTAGCGGTTTGGGCACAAAAGCAGATAAAGGAGTAG

Upstream 100 bases:

>100_bases
ATATTCTTGAAGACGCCAGTGATGAAGTGATTGGTCACATTAAAGAATTTATGGCAAAAACAGAGCAGCAAACTGCCACT
GAGTCGGTGGCGTAAACTCG

Downstream 100 bases:

>100_bases
TGCACTTTGTCATTAGCTAATAAGAATCAAACTCCTGTGCTTGAGGACTTCTTACCCGAAGAACAGGCCGCGCTGTTGGC
CCTGAAGCAAAAGGTGAATC

Product: peptide synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MVSQLTQKNDGANLCRHLVSAAQTIPDSLAVAVQQSKGRAVSGLRYQEMDFQTLNAKSDELASALISHGLMPGMKAVLMV
TPSIDFFCLTFALFKAGIIPILVDPGMGVKNLKQCFIEAKPDAFIGIPKAHIARRLFGWGKPTVKHLINVGGSAFAQALT
GATSLESLLKANRSPTTVPFNMQWLDEDAMAAILFTSGSTGTPKGVVYSHKMFEAQITALKHDYAIKPGERDLATFPLFS
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGQAGEAKQHKLASIKRVISAGAPATISSIK
RFSQMLNQDVEVLNSYGATESLPISKIGSQALFDTTFVTDNGGGICVGKAIDGVEIAIIAIDEQPIIHWHDSLALPVDAI
GEIVVKGPMVSRSYYQRESATQVAKIQDGASIRHRMGDLGYLDSDGLLWMCGRKAHRVDADALPATNNQAQRYYSIPCER
IFNTHSDVKRSALVGVKVAGRVTPLVCIELKQSVACSLSGVLYSELRAIAEQHAQTQGIDRFFIHPDFPVDIRHNAKIFR
EQLAVWAQKQIKE

Sequences:

>Translated_573_residues
MVSQLTQKNDGANLCRHLVSAAQTIPDSLAVAVQQSKGRAVSGLRYQEMDFQTLNAKSDELASALISHGLMPGMKAVLMV
TPSIDFFCLTFALFKAGIIPILVDPGMGVKNLKQCFIEAKPDAFIGIPKAHIARRLFGWGKPTVKHLINVGGSAFAQALT
GATSLESLLKANRSPTTVPFNMQWLDEDAMAAILFTSGSTGTPKGVVYSHKMFEAQITALKHDYAIKPGERDLATFPLFS
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGQAGEAKQHKLASIKRVISAGAPATISSIK
RFSQMLNQDVEVLNSYGATESLPISKIGSQALFDTTFVTDNGGGICVGKAIDGVEIAIIAIDEQPIIHWHDSLALPVDAI
GEIVVKGPMVSRSYYQRESATQVAKIQDGASIRHRMGDLGYLDSDGLLWMCGRKAHRVDADALPATNNQAQRYYSIPCER
IFNTHSDVKRSALVGVKVAGRVTPLVCIELKQSVACSLSGVLYSELRAIAEQHAQTQGIDRFFIHPDFPVDIRHNAKIFR
EQLAVWAQKQIKE
>Mature_573_residues
MVSQLTQKNDGANLCRHLVSAAQTIPDSLAVAVQQSKGRAVSGLRYQEMDFQTLNAKSDELASALISHGLMPGMKAVLMV
TPSIDFFCLTFALFKAGIIPILVDPGMGVKNLKQCFIEAKPDAFIGIPKAHIARRLFGWGKPTVKHLINVGGSAFAQALT
GATSLESLLKANRSPTTVPFNMQWLDEDAMAAILFTSGSTGTPKGVVYSHKMFEAQITALKHDYAIKPGERDLATFPLFS
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGQAGEAKQHKLASIKRVISAGAPATISSIK
RFSQMLNQDVEVLNSYGATESLPISKIGSQALFDTTFVTDNGGGICVGKAIDGVEIAIIAIDEQPIIHWHDSLALPVDAI
GEIVVKGPMVSRSYYQRESATQVAKIQDGASIRHRMGDLGYLDSDGLLWMCGRKAHRVDADALPATNNQAQRYYSIPCER
IFNTHSDVKRSALVGVKVAGRVTPLVCIELKQSVACSLSGVLYSELRAIAEQHAQTQGIDRFFIHPDFPVDIRHNAKIFR
EQLAVWAQKQIKE

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=474, Percent_Identity=21.7299578059072, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693145, Length=461, Percent_Identity=23.2104121475054, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=461, Percent_Identity=23.4273318872017, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=461, Percent_Identity=23.4273318872017, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=472, Percent_Identity=23.5169491525424, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=279, Percent_Identity=24.7311827956989, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=332, Percent_Identity=23.1927710843373, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356947, Length=309, Percent_Identity=23.9482200647249, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI18859661, Length=277, Percent_Identity=24.5487364620939, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 62180; Mature: 62180

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSQLTQKNDGANLCRHLVSAAQTIPDSLAVAVQQSKGRAVSGLRYQEMDFQTLNAKSDE
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEECCCEEECCCCCCCCCCHHH
LASALISHGLMPGMKAVLMVTPSIDFFCLTFALFKAGIIPILVDPGMGVKNLKQCFIEAK
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCC
PDAFIGIPKAHIARRLFGWGKPTVKHLINVGGSAFAQALTGATSLESLLKANRSPTTVPF
CCCEEECCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCCCEEEE
NMQWLDEDAMAAILFTSGSTGTPKGVVYSHKMFEAQITALKHDYAIKPGERDLATFPLFS
EEEECCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGQAGEAKQHK
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHCCCCEEECHHHHHHHCCCCCHHHHH
LASIKRVISAGAPATISSIKRFSQMLNQDVEVLNSYGATESLPISKIGSQALFDTTFVTD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHEEEEEEEC
NGGGICVGKAIDGVEIAIIAIDEQPIIHWHDSLALPVDAIGEIVVKGPMVSRSYYQRESA
CCCCEEEECCCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
TQVAKIQDGASIRHRMGDLGYLDSDGLLWMCGRKAHRVDADALPATNNQAQRYYSIPCER
HHHHHHCCCHHHHHHCCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHEECCCHHH
IFNTHSDVKRSALVGVKVAGRVTPLVCIELKQSVACSLSGVLYSELRAIAEQHAQTQGID
HHCCHHHHHHHHHHEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RFFIHPDFPVDIRHNAKIFREQLAVWAQKQIKE
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSQLTQKNDGANLCRHLVSAAQTIPDSLAVAVQQSKGRAVSGLRYQEMDFQTLNAKSDE
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEECCCEEECCCCCCCCCCHHH
LASALISHGLMPGMKAVLMVTPSIDFFCLTFALFKAGIIPILVDPGMGVKNLKQCFIEAK
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCC
PDAFIGIPKAHIARRLFGWGKPTVKHLINVGGSAFAQALTGATSLESLLKANRSPTTVPF
CCCEEECCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCCCEEEE
NMQWLDEDAMAAILFTSGSTGTPKGVVYSHKMFEAQITALKHDYAIKPGERDLATFPLFS
EEEECCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGQAGEAKQHK
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHCCCCEEECHHHHHHHCCCCCHHHHH
LASIKRVISAGAPATISSIKRFSQMLNQDVEVLNSYGATESLPISKIGSQALFDTTFVTD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHEEEEEEEC
NGGGICVGKAIDGVEIAIIAIDEQPIIHWHDSLALPVDAIGEIVVKGPMVSRSYYQRESA
CCCCEEEECCCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
TQVAKIQDGASIRHRMGDLGYLDSDGLLWMCGRKAHRVDADALPATNNQAQRYYSIPCER
HHHHHHCCCHHHHHHCCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHEECCCHHH
IFNTHSDVKRSALVGVKVAGRVTPLVCIELKQSVACSLSGVLYSELRAIAEQHAQTQGID
HHCCHHHHHHHHHHEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RFFIHPDFPVDIRHNAKIFREQLAVWAQKQIKE
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]