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The map label for this gene is ybjS [C]

Identifier: 167624734

GI number: 167624734

Start: 3424895

End: 3425998

Strand: Reverse

Name: ybjS [C]

Synonym: Shal_2817

Alternate gene names: 167624734

Gene position: 3425998-3424895 (Counterclockwise)

Preceding gene: 167624735

Following gene: 167624733

Centisome position: 65.55

GC content: 48.55

Gene sequence:

>1104_bases
TTGTCATTAGCTAATAAGAATCAAACTCCTGTGCTTGAGGACTTCTTACCCGAAGAACAGGCCGCGCTGTTGGCCCTGAA
GCAAAAGGTGAATCGTGCCTTTGTAACCGGAGCTGGGGGCTTCTTAGGTAAAGCCATTTGTGAGCGTTTACTTGCCGCAG
GCATTAACGTCACAGGCTTTGCACGGAGTGAATATCCCGAGTTAAGCGCAATGGGCGTGAATATGATCAAAGGCGATATC
GCCAATGAGCAAGCCCTGCTCGATGCTATACAGGGCTGTGATGTGGTGTTTCATGTGGCATCCAAAGCCGGAGTTTGGGG
CAGTAAACAAAGCTATTTTTCACCCAATGTCGATGGTGCTCACCATATTATTAATGCCTGTAAAAAGTGCAATATTGAGT
GCATCGTGTATACCAGCACCCCGAGCGTCACCTTTGCCGGCGCGGATGAAGAGGGCAATGATGAATCTGCTCCCTATGCT
AAGCAGTTTCTCAATTTCTATGGCGAATCGAAAGCGATTGCCGAGCAGATGATGCTAGCTGCCAATGGCGACAAGCTCAG
TAATGGCAAATACCTTGGTACCACAGCGCTGCGTCCGCATCTTATTTGGGGGCCGAATGATCCTCATTTGGTGCCGAGAG
TGCTTGAACGTGCCGCCGCTGGCAGGTTAAAGCTGGTAGGAAAAGAAGATAAGCTGGTCGATACTATTTATGTGGGCAAT
GCTGCCTATGCCCATATTCTGGCGGCGCTTAAGCTGAGCGAGACGAGTGCTGATAGAGCTAGCTGCGCAGGTAGGGCTTA
TTACCTGAGTAATGATGAGCCCATCACAATGGCGGCCATGCTCAATAAAATTCTCGAATGTAAAGATCTCCCGCCTGTGA
CTAAGCGAGTGCCAAGCAATGTGGCTTATATTGCAGGCTGTGTGCTTGAGTCAGTATATGGGTTATTAGGCAAAAAAGAT
GAGCCGATCATGACTCGTTTTGTCGCGCGCCAGCTTTCAACCAGTCATTACTTCGATATTAGCGCGGCCAAAAATGATTT
AGGCTACAGCGCCTTAGTGAGTATTGATGAGGGAATGGAGCGACTTAAAGCCTCTTTACAATAA

Upstream 100 bases:

>100_bases
CATTCACCCCGACTTTCCGGTGGATATTCGCCATAATGCCAAAATTTTCCGTGAACAATTAGCGGTTTGGGCACAAAAGC
AGATAAAGGAGTAGTGCACT

Downstream 100 bases:

>100_bases
ACAAGGGTAGATTGATACTGACGACTAAGTGTAAAAAAGCAGCAATATTCTTGCTGCTTTTTTATACCCATAGATTAATT
ATGTTCTCAAGTGACTGATA

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSLANKNQTPVLEDFLPEEQAALLALKQKVNRAFVTGAGGFLGKAICERLLAAGINVTGFARSEYPELSAMGVNMIKGDI
ANEQALLDAIQGCDVVFHVASKAGVWGSKQSYFSPNVDGAHHIINACKKCNIECIVYTSTPSVTFAGADEEGNDESAPYA
KQFLNFYGESKAIAEQMMLAANGDKLSNGKYLGTTALRPHLIWGPNDPHLVPRVLERAAAGRLKLVGKEDKLVDTIYVGN
AAYAHILAALKLSETSADRASCAGRAYYLSNDEPITMAAMLNKILECKDLPPVTKRVPSNVAYIAGCVLESVYGLLGKKD
EPIMTRFVARQLSTSHYFDISAAKNDLGYSALVSIDEGMERLKASLQ

Sequences:

>Translated_367_residues
MSLANKNQTPVLEDFLPEEQAALLALKQKVNRAFVTGAGGFLGKAICERLLAAGINVTGFARSEYPELSAMGVNMIKGDI
ANEQALLDAIQGCDVVFHVASKAGVWGSKQSYFSPNVDGAHHIINACKKCNIECIVYTSTPSVTFAGADEEGNDESAPYA
KQFLNFYGESKAIAEQMMLAANGDKLSNGKYLGTTALRPHLIWGPNDPHLVPRVLERAAAGRLKLVGKEDKLVDTIYVGN
AAYAHILAALKLSETSADRASCAGRAYYLSNDEPITMAAMLNKILECKDLPPVTKRVPSNVAYIAGCVLESVYGLLGKKD
EPIMTRFVARQLSTSHYFDISAAKNDLGYSALVSIDEGMERLKASLQ
>Mature_366_residues
SLANKNQTPVLEDFLPEEQAALLALKQKVNRAFVTGAGGFLGKAICERLLAAGINVTGFARSEYPELSAMGVNMIKGDIA
NEQALLDAIQGCDVVFHVASKAGVWGSKQSYFSPNVDGAHHIINACKKCNIECIVYTSTPSVTFAGADEEGNDESAPYAK
QFLNFYGESKAIAEQMMLAANGDKLSNGKYLGTTALRPHLIWGPNDPHLVPRVLERAAAGRLKLVGKEDKLVDTIYVGNA
AYAHILAALKLSETSADRASCAGRAYYLSNDEPITMAAMLNKILECKDLPPVTKRVPSNVAYIAGCVLESVYGLLGKKDE
PIMTRFVARQLSTSHYFDISAAKNDLGYSALVSIDEGMERLKASLQ

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=360, Percent_Identity=33.6111111111111, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI8393516, Length=360, Percent_Identity=33.6111111111111, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI310132178, Length=349, Percent_Identity=30.945558739255, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI310113012, Length=349, Percent_Identity=30.945558739255, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI239745448, Length=349, Percent_Identity=30.945558739255, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI116268111, Length=346, Percent_Identity=32.3699421965318, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI260763931, Length=350, Percent_Identity=31.7142857142857, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI4504509, Length=350, Percent_Identity=31.7142857142857, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI4504507, Length=350, Percent_Identity=32, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI310113808, Length=255, Percent_Identity=30.9803921568627, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310127990, Length=255, Percent_Identity=30.5882352941176, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI19923621, Length=260, Percent_Identity=31.5384615384615, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI87081792, Length=348, Percent_Identity=26.7241379310345, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1786974, Length=258, Percent_Identity=25.968992248062, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17570557, Length=331, Percent_Identity=25.3776435045317, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI193211272, Length=343, Percent_Identity=22.7405247813411, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17509709, Length=295, Percent_Identity=23.0508474576271, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321437, Length=354, Percent_Identity=32.2033898305085, Blast_Score=153, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 39529; Mature: 39397

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLANKNQTPVLEDFLPEEQAALLALKQKVNRAFVTGAGGFLGKAICERLLAAGINVTGF
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCEEC
ARSEYPELSAMGVNMIKGDIANEQALLDAIQGCDVVFHVASKAGVWGSKQSYFSPNVDGA
CCCCCCCHHHCCHHHHHCCCCCHHHHHHHHHCCHHEEEHHHHCCCCCCCCCCCCCCCCHH
HHIINACKKCNIECIVYTSTPSVTFAGADEEGNDESAPYAKQFLNFYGESKAIAEQMMLA
HHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
ANGDKLSNGKYLGTTALRPHLIWGPNDPHLVPRVLERAAAGRLKLVGKEDKLVDTIYVGN
CCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEECC
AAYAHILAALKLSETSADRASCAGRAYYLSNDEPITMAAMLNKILECKDLPPVTKRVPSN
HHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCCEEHHHHHHHHHHHCCCCHHHHHCCCC
VAYIAGCVLESVYGLLGKKDEPIMTRFVARQLSTSHYFDISAAKNDLGYSALVSIDEGME
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
RLKASLQ
HHHHHCC
>Mature Secondary Structure 
SLANKNQTPVLEDFLPEEQAALLALKQKVNRAFVTGAGGFLGKAICERLLAAGINVTGF
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCEEC
ARSEYPELSAMGVNMIKGDIANEQALLDAIQGCDVVFHVASKAGVWGSKQSYFSPNVDGA
CCCCCCCHHHCCHHHHHCCCCCHHHHHHHHHCCHHEEEHHHHCCCCCCCCCCCCCCCCHH
HHIINACKKCNIECIVYTSTPSVTFAGADEEGNDESAPYAKQFLNFYGESKAIAEQMMLA
HHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
ANGDKLSNGKYLGTTALRPHLIWGPNDPHLVPRVLERAAAGRLKLVGKEDKLVDTIYVGN
CCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEECC
AAYAHILAALKLSETSADRASCAGRAYYLSNDEPITMAAMLNKILECKDLPPVTKRVPSN
HHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCCEEHHHHHHHHHHHCCCCHHHHHCCCC
VAYIAGCVLESVYGLLGKKDEPIMTRFVARQLSTSHYFDISAAKNDLGYSALVSIDEGME
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
RLKASLQ
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]