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The map label for this gene is aes [H]

Identifier: 167624653

GI number: 167624653

Start: 3317648

End: 3318583

Strand: Reverse

Name: aes [H]

Synonym: Shal_2735

Alternate gene names: 167624653

Gene position: 3318583-3317648 (Counterclockwise)

Preceding gene: 167624654

Following gene: 167624647

Centisome position: 63.49

GC content: 41.77

Gene sequence:

>936_bases
ATGAGAAATGTAAGTGAAAAACTCAAACCTTGGCTAGATAACTTTAATACACAAATAGCAGTTTTAATTGAAAACGGCTT
TAAGCCAACAGCGACTAATGCACGTGAAGGTTTAGCTAATCTGACGAAAGGTTTAGTGACAAATATTCCTGATATTGCAT
GGGTAGCCGACGATTTGGTCATGAATGAACAGTACAATGTACCCGTACGTATTTATCATCCGGTGCCCACTAAAAAATTA
CCTGTAATTGTGTATTTTCATGGTGGTGGACACATGGCTGGTAGTGTCACGGTTTACGATCCCATTTGCCGTAAACTTGC
CAATGCGACTCAACATATCGTGGTATCGGTTGACTATAGACTGGCACCAGAGTGCCCATATCCTGCTGGCGTGAATGACG
GTTATGCAGTAGTTGAAGGGATCTGGGCAGTACTCGATGGACGTTCAGTTAATTATCAGAAGCAACTATCCGTAGTCGGA
GATTCTGGTGGTGGCGCATTGGTGGCTAGCGTAAGCGGACGAGCTCAATTTGATAAGTCGGTGATTATTGACAAACAGGT
AATGATATACCCAAGCCTTGATTACACAATGGACACTCCTTCAATGGATCAAAATGCCGTTGGGTACTTATTACAAAAAG
GCAAAATTGGCTGGTATTTTGATAACTATTTTAGTGAAGCTGACGACAGAGTCGCTGCATCCCCACTGCATGGTAATTTA
ACCCGCGCATTACCGGAAACGTTAGTATTTACTGCTGAATTCTGTCCATTAAGAGATGAAGGCAAAATGTACGTAGACAA
AGTTAAGCAAGCGGGTGGTAAAGCAGAGTTGATACACTTTGATGATATGATCCACACGTTTTTGAACATGGAAGACCTTG
TTCAAGCTGAGTGCGATTTAGTTTATCGAACAATGGGTGAGTTTTTGAATCGATAA

Upstream 100 bases:

>100_bases
TTCATCTACCTTCTTTATTGCTAGCTTCTTTTTACCTGAAACCGGTAAAAGAAAACCTGTTCCATCATCAGTCACTGTAA
CGAAATAATTGGGAAGTATT

Downstream 100 bases:

>100_bases
TTGCTTTCAACCATTGTTTTGACTAACAAAAAAGACCGTTTATAGATTCTATTCGGTCTTTTTTCTTTAAAATTCAAAAC
TGCTGATATTAGAATTTAAG

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRNVSEKLKPWLDNFNTQIAVLIENGFKPTATNAREGLANLTKGLVTNIPDIAWVADDLVMNEQYNVPVRIYHPVPTKKL
PVIVYFHGGGHMAGSVTVYDPICRKLANATQHIVVSVDYRLAPECPYPAGVNDGYAVVEGIWAVLDGRSVNYQKQLSVVG
DSGGGALVASVSGRAQFDKSVIIDKQVMIYPSLDYTMDTPSMDQNAVGYLLQKGKIGWYFDNYFSEADDRVAASPLHGNL
TRALPETLVFTAEFCPLRDEGKMYVDKVKQAGGKAELIHFDDMIHTFLNMEDLVQAECDLVYRTMGEFLNR

Sequences:

>Translated_311_residues
MRNVSEKLKPWLDNFNTQIAVLIENGFKPTATNAREGLANLTKGLVTNIPDIAWVADDLVMNEQYNVPVRIYHPVPTKKL
PVIVYFHGGGHMAGSVTVYDPICRKLANATQHIVVSVDYRLAPECPYPAGVNDGYAVVEGIWAVLDGRSVNYQKQLSVVG
DSGGGALVASVSGRAQFDKSVIIDKQVMIYPSLDYTMDTPSMDQNAVGYLLQKGKIGWYFDNYFSEADDRVAASPLHGNL
TRALPETLVFTAEFCPLRDEGKMYVDKVKQAGGKAELIHFDDMIHTFLNMEDLVQAECDLVYRTMGEFLNR
>Mature_311_residues
MRNVSEKLKPWLDNFNTQIAVLIENGFKPTATNAREGLANLTKGLVTNIPDIAWVADDLVMNEQYNVPVRIYHPVPTKKL
PVIVYFHGGGHMAGSVTVYDPICRKLANATQHIVVSVDYRLAPECPYPAGVNDGYAVVEGIWAVLDGRSVNYQKQLSVVG
DSGGGALVASVSGRAQFDKSVIIDKQVMIYPSLDYTMDTPSMDQNAVGYLLQKGKIGWYFDNYFSEADDRVAASPLHGNL
TRALPETLVFTAEFCPLRDEGKMYVDKVKQAGGKAELIHFDDMIHTFLNMEDLVQAECDLVYRTMGEFLNR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=292, Percent_Identity=28.0821917808219, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI68299767, Length=298, Percent_Identity=25.503355704698, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68051721, Length=292, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157041239, Length=182, Percent_Identity=30.2197802197802, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI226423947, Length=300, Percent_Identity=23.6666666666667, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786682, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567059, Length=335, Percent_Identity=25.0746268656716, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71996133, Length=133, Percent_Identity=30.8270676691729, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=171, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=134, Percent_Identity=32.8358208955224, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34503; Mature: 34503

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVSEKLKPWLDNFNTQIAVLIENGFKPTATNAREGLANLTKGLVTNIPDIAWVADDLV
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
MNEQYNVPVRIYHPVPTKKLPVIVYFHGGGHMAGSVTVYDPICRKLANATQHIVVSVDYR
CCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHCCCCEEEEEEEEEE
LAPECPYPAGVNDGYAVVEGIWAVLDGRSVNYQKQLSVVGDSGGGALVASVSGRAQFDKS
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCE
VIIDKQVMIYPSLDYTMDTPSMDQNAVGYLLQKGKIGWYFDNYFSEADDRVAASPLHGNL
EEECCEEEEEECCCCEECCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCEECCCCCCCH
TRALPETLVFTAEFCPLRDEGKMYVDKVKQAGGKAELIHFDDMIHTFLNMEDLVQAECDL
HHHHHHHHEEEHHCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHCHHHHHHHHHHH
VYRTMGEFLNR
HHHHHHHHHCC
>Mature Secondary Structure
MRNVSEKLKPWLDNFNTQIAVLIENGFKPTATNAREGLANLTKGLVTNIPDIAWVADDLV
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
MNEQYNVPVRIYHPVPTKKLPVIVYFHGGGHMAGSVTVYDPICRKLANATQHIVVSVDYR
CCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHCCCCEEEEEEEEEE
LAPECPYPAGVNDGYAVVEGIWAVLDGRSVNYQKQLSVVGDSGGGALVASVSGRAQFDKS
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCE
VIIDKQVMIYPSLDYTMDTPSMDQNAVGYLLQKGKIGWYFDNYFSEADDRVAASPLHGNL
EEECCEEEEEECCCCEECCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCEECCCCCCCH
TRALPETLVFTAEFCPLRDEGKMYVDKVKQAGGKAELIHFDDMIHTFLNMEDLVQAECDL
HHHHHHHHEEEHHCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHCHHHHHHHHHHH
VYRTMGEFLNR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA