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The map label for this gene is pdxB

Identifier: 167624564

GI number: 167624564

Start: 3207557

End: 3208687

Strand: Reverse

Name: pdxB

Synonym: Shal_2644

Alternate gene names: 167624564

Gene position: 3208687-3207557 (Counterclockwise)

Preceding gene: 167624566

Following gene: 167624563

Centisome position: 61.39

GC content: 46.24

Gene sequence:

>1131_bases
ATGAAGATCCTTGCCGATGAAAATATGCCTTATGTACAACAGTTATTTGGTGATTTGGGCACGATTGAAACCGTAAACGG
TAGGGAGTTAACGCCTGAGCAAGTAAAAGATGCCGATGTACTGCTAGTGCGCTCAGTGACTAAGGTCGATCAAGCATTGT
TAGCAGAGAATAACCGACTTAAATTTGTCGGCAGTGCCACCATAGGTACAGATCATATCGATTTAGATTATCTTGCAAGT
CACAATATTCCCTTCTCTAATGCGCCGGGCTGTAACGCCACTGCTGTGGGGGAGTTTGCCTTTATCGCCATGCTTGAGTT
GGCGCAGCGCTTTAATAGCCCACTTAAAGGCAAGGTGGTGGGTATTGTTGGTGCGGGTAATACCGGCACTGCGGTGGTTA
AATGCCTTGAGGCTTATGGCATTGAAGTACTGCTTAATGATCCACTGTTAGAGCAAAGTGGCGATCCCCGCGACTTTGTG
TCGTTAGATACGCTTATTGAAAAGTGCGATGTTATTAGCCTGCATGTGCCGATAACTAAAACTGGTGAGCACAAAACTTG
GTATCTGTTCGATGAGCAGAGGCTCAATAGCCTAGCTGAAAACACCTGGCTGGTTAACTGTTGCCGCGGTGAGGTGATAG
ATAATCGTGCCCTGATTAAATTCAAACAACAGCGTGATGACGTCAAAGTGGTGCTCGATGTGTGGGAAGGCGAGCCCAAC
CCTATGCCTGAGTTGGTGCCCTATGTTGAGTTTTGTACGCCCCATATCGCCGGTTACTCGTTAGAGGGTAAGGCGCGCGG
TACCTATATCTTGTATCAAAAATTGGCTGAGGTGCTTCAAATTAGCGCCGACAAACAGATGGAGTCGTTACTGCCAGCGC
TGTGGAGTGAGCGGGTCTTAGTGCAGGAAATTAGCGATGAAAGAGCGTTATTACAGCTGGCGAGGTTTGTCTATGATCTG
CGCGATGATGACGAATTATTTAGAAAACAGTTTCTAAATAACCAGGGGTTTGATCATATGAGAAAAAATCACACCCATCG
GCGCGAATTTAGTGCATTAAAGGTAGGCAACACGGGGCAAACTAATGTAAATTGGTTATCCAATTTAGGGTTTTCAGGAG
TCGAGCTGTAA

Upstream 100 bases:

>100_bases
GTTTATTTTCAATGCTATTTTCAATGCTATTGTTTCTAATAAATTGACCATCTTGTTAGAATGCAGTCCATACGCATTCA
GGCCACTTTAGGGAACCACA

Downstream 100 bases:

>100_bases
TTATGTCGCAGGAATTTAATGTTGTCGTATTAGGTGCATCGGGCGCAGTGGGTCAAACAATGATTGAGATCCTCGAAGAG
CGTAACTTTCCGGTTGCTAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKILADENMPYVQQLFGDLGTIETVNGRELTPEQVKDADVLLVRSVTKVDQALLAENNRLKFVGSATIGTDHIDLDYLAS
HNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVVKCLEAYGIEVLLNDPLLEQSGDPRDFV
SLDTLIEKCDVISLHVPITKTGEHKTWYLFDEQRLNSLAENTWLVNCCRGEVIDNRALIKFKQQRDDVKVVLDVWEGEPN
PMPELVPYVEFCTPHIAGYSLEGKARGTYILYQKLAEVLQISADKQMESLLPALWSERVLVQEISDERALLQLARFVYDL
RDDDELFRKQFLNNQGFDHMRKNHTHRREFSALKVGNTGQTNVNWLSNLGFSGVEL

Sequences:

>Translated_376_residues
MKILADENMPYVQQLFGDLGTIETVNGRELTPEQVKDADVLLVRSVTKVDQALLAENNRLKFVGSATIGTDHIDLDYLAS
HNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVVKCLEAYGIEVLLNDPLLEQSGDPRDFV
SLDTLIEKCDVISLHVPITKTGEHKTWYLFDEQRLNSLAENTWLVNCCRGEVIDNRALIKFKQQRDDVKVVLDVWEGEPN
PMPELVPYVEFCTPHIAGYSLEGKARGTYILYQKLAEVLQISADKQMESLLPALWSERVLVQEISDERALLQLARFVYDL
RDDDELFRKQFLNNQGFDHMRKNHTHRREFSALKVGNTGQTNVNWLSNLGFSGVEL
>Mature_376_residues
MKILADENMPYVQQLFGDLGTIETVNGRELTPEQVKDADVLLVRSVTKVDQALLAENNRLKFVGSATIGTDHIDLDYLAS
HNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVVKCLEAYGIEVLLNDPLLEQSGDPRDFV
SLDTLIEKCDVISLHVPITKTGEHKTWYLFDEQRLNSLAENTWLVNCCRGEVIDNRALIKFKQQRDDVKVVLDVWEGEPN
PMPELVPYVEFCTPHIAGYSLEGKARGTYILYQKLAEVLQISADKQMESLLPALWSERVLVQEISDERALLQLARFVYDL
RDDDELFRKQFLNNQGFDHMRKNHTHRREFSALKVGNTGQTNVNWLSNLGFSGVEL

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=290, Percent_Identity=24.4827586206897, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI145580578, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4557499, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI145580575, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557497, Length=158, Percent_Identity=29.746835443038, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI61743967, Length=158, Percent_Identity=29.746835443038, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788660, Length=375, Percent_Identity=44.8, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1789279, Length=263, Percent_Identity=28.5171102661597, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=25.6281407035176, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082289, Length=319, Percent_Identity=23.5109717868339, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=275, Percent_Identity=26.5454545454545, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320925, Length=267, Percent_Identity=27.3408239700375, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=267, Percent_Identity=27.3408239700375, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=285, Percent_Identity=22.4561403508772, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324964, Length=280, Percent_Identity=22.1428571428571, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921140, Length=262, Percent_Identity=25.1908396946565, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646446, Length=238, Percent_Identity=26.0504201680672, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646448, Length=238, Percent_Identity=26.0504201680672, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646452, Length=238, Percent_Identity=26.0504201680672, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646450, Length=238, Percent_Identity=26.0504201680672, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62472511, Length=229, Percent_Identity=26.6375545851528, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_SHEHH (B0TKZ7)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674858.1
- ProteinModelPortal:   B0TKZ7
- SMR:   B0TKZ7
- GeneID:   5905883
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2644
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- ProtClustDB:   CLSK906984
- BioCyc:   SHAL458817:SHAL_2644-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 42182; Mature: 42182

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 209-209 ACT_SITE 238-238 ACT_SITE 255-255 BINDING 45-45 BINDING 67-67 BINDING 147-147 BINDING 233-233 BINDING 258-258 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILADENMPYVQQLFGDLGTIETVNGRELTPEQVKDADVLLVRSVTKVDQALLAENNRL
CCEECCCCCCHHHHHHCCCCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCE
KFVGSATIGTDHIDLDYLASHNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVV
EEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGTAVVKCLEAYGIEVLLNDPLLEQSGDPRDFVSLDTLIEKCDVISLHVPITK
EEEECCCCHHHHHHHHHHCCEEEEECCCHHHCCCCCHHHHHHHHHHHHCCEEEEEECEEE
TGEHKTWYLFDEQRLNSLAENTWLVNCCRGEVIDNRALIKFKQQRDDVKVVLDVWEGEPN
CCCCCEEEEECHHHHHHHHHCCEEEEECCCCEECCHHHEEECCCCCCEEEEEEEECCCCC
PMPELVPYVEFCTPHIAGYSLEGKARGTYILYQKLAEVLQISADKQMESLLPALWSERVL
CCHHHHHHHHHHCHHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHH
VQEISDERALLQLARFVYDLRDDDELFRKQFLNNQGFDHMRKNHTHRREFSALKVGNTGQ
HHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCHHHHHHEEECCCCCC
TNVNWLSNLGFSGVEL
CCHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILADENMPYVQQLFGDLGTIETVNGRELTPEQVKDADVLLVRSVTKVDQALLAENNRL
CCEECCCCCCHHHHHHCCCCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCE
KFVGSATIGTDHIDLDYLASHNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVV
EEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGTAVVKCLEAYGIEVLLNDPLLEQSGDPRDFVSLDTLIEKCDVISLHVPITK
EEEECCCCHHHHHHHHHHCCEEEEECCCHHHCCCCCHHHHHHHHHHHHCCEEEEEECEEE
TGEHKTWYLFDEQRLNSLAENTWLVNCCRGEVIDNRALIKFKQQRDDVKVVLDVWEGEPN
CCCCCEEEEECHHHHHHHHHCCEEEEECCCCEECCHHHEEECCCCCCEEEEEEEECCCCC
PMPELVPYVEFCTPHIAGYSLEGKARGTYILYQKLAEVLQISADKQMESLLPALWSERVL
CCHHHHHHHHHHCHHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHH
VQEISDERALLQLARFVYDLRDDDELFRKQFLNNQGFDHMRKNHTHRREFSALKVGNTGQ
HHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCHHHHHHEEECCCCCC
TNVNWLSNLGFSGVEL
CCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA