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The map label for this gene is nadE

Identifier: 167624439

GI number: 167624439

Start: 3037532

End: 3038362

Strand: Reverse

Name: nadE

Synonym: Shal_2517

Alternate gene names: 167624439

Gene position: 3038362-3037532 (Counterclockwise)

Preceding gene: 167624441

Following gene: 167624438

Centisome position: 58.13

GC content: 46.09

Gene sequence:

>831_bases
GTGAAAGGACAGATCTTAAGGGAAATGAAGGTGCTTAAAGCAATCGATCCCGCTTTTGAGGTGCAAAGACGTGTGGCATT
TATTAAGTCAAAATTAAAGCAATCGAGTACAACGTCACTGGTGTTAGGGATCAGTGGGGGGGTCGACTCCTCTGTGGGGG
GACGTTTGTGTCAGCTTGCGGTTGATGAGCTCAATAGCGAGTCTCAGAGCAGTAGCTACCAGTTTATAGCGGTTCGCCTG
CCTTATGATGTACAAAAAGATGAAGACGAAGCGCAGTTGGCTTGTCAGTTTATTCAGCCTTCAAAGCAAGTGACGGTAAA
TGTAAAGCTTGGTGTTGATGGCGTTCATAGTGAAACACTCGCGGCGATTGAAGCTGCAGGCATAGCCTTACCTGAGCATG
ATAAAATCGACTTTGTAAAGGGCAACGTGAAGGCAAGAATGCGCATGGTTGCTCAATATGATATTGCAGGACTTGTAGGC
GGGCTCGTGGTTGGCACCGATCACAGCGCCGAAAATATTACCGGCTTTTACACTAAGTGGGGTGATGGTGCATGTGATTT
AGCGCCGCTGTTTGGTCTCAATAAACGTCAGGTAAGACAGTTGGCAGACTATCTTGGCGCACCGGAAGTATTAGTGAGCA
AAGCGCCAACCGCTGATCTTGAAGAGGAACATCCGCAACAAGAAGATGAAGAGGCGTTGGGGCTAACATACGAGCAGATT
GATGACTTTTTAGAAGGTAAAGTGGTCAGTAATTTTGTTAATGATAAATTGATCAGTATGTACCTTGCGACGCAGCATAA
ACGTGAACCAATTCCTACGATCTATGATTAG

Upstream 100 bases:

>100_bases
TAAATTAACACGATTGTTATTAAACAGGTCGTGAATTGTCGCAAGAATTCACCCATAATATAAACCAATTTTTATGCCAA
CCACCATCGGAGTGTAGAAC

Downstream 100 bases:

>100_bases
GCTAATGGCGGCGCGGGTACCGATAGGTATTCGACGCTATTGATATACGAAAGACGCCTAGGCGTCTTTTTCTTTAGGCC
GCTTTTAAGGATGATACTAA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKGQILREMKVLKAIDPAFEVQRRVAFIKSKLKQSSTTSLVLGISGGVDSSVGGRLCQLAVDELNSESQSSSYQFIAVRL
PYDVQKDEDEAQLACQFIQPSKQVTVNVKLGVDGVHSETLAAIEAAGIALPEHDKIDFVKGNVKARMRMVAQYDIAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLADYLGAPEVLVSKAPTADLEEEHPQQEDEEALGLTYEQI
DDFLEGKVVSNFVNDKLISMYLATQHKREPIPTIYD

Sequences:

>Translated_276_residues
MKGQILREMKVLKAIDPAFEVQRRVAFIKSKLKQSSTTSLVLGISGGVDSSVGGRLCQLAVDELNSESQSSSYQFIAVRL
PYDVQKDEDEAQLACQFIQPSKQVTVNVKLGVDGVHSETLAAIEAAGIALPEHDKIDFVKGNVKARMRMVAQYDIAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLADYLGAPEVLVSKAPTADLEEEHPQQEDEEALGLTYEQI
DDFLEGKVVSNFVNDKLISMYLATQHKREPIPTIYD
>Mature_276_residues
MKGQILREMKVLKAIDPAFEVQRRVAFIKSKLKQSSTTSLVLGISGGVDSSVGGRLCQLAVDELNSESQSSSYQFIAVRL
PYDVQKDEDEAQLACQFIQPSKQVTVNVKLGVDGVHSETLAAIEAAGIALPEHDKIDFVKGNVKARMRMVAQYDIAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLADYLGAPEVLVSKAPTADLEEEHPQQEDEEALGLTYEQI
DDFLEGKVVSNFVNDKLISMYLATQHKREPIPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=51.6245487364621, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_SHEHH (B0TK55)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674733.1
- ProteinModelPortal:   B0TK55
- SMR:   B0TK55
- GeneID:   5906407
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2517
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   SHAL458817:SHAL_2517-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30229; Mature: 30229

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGQILREMKVLKAIDPAFEVQRRVAFIKSKLKQSSTTSLVLGISGGVDSSVGGRLCQLA
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
VDELNSESQSSSYQFIAVRLPYDVQKDEDEAQLACQFIQPSKQVTVNVKLGVDGVHSETL
HHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
AAIEAAGIALPEHDKIDFVKGNVKARMRMVAQYDIAGLVGGLVVGTDHSAENITGFYTKW
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLADYLGAPEVLVSKAPTADLEEEHPQQEDEEALGLTYEQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCCCHHHHCCCHHHH
DDFLEGKVVSNFVNDKLISMYLATQHKREPIPTIYD
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKGQILREMKVLKAIDPAFEVQRRVAFIKSKLKQSSTTSLVLGISGGVDSSVGGRLCQLA
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
VDELNSESQSSSYQFIAVRLPYDVQKDEDEAQLACQFIQPSKQVTVNVKLGVDGVHSETL
HHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
AAIEAAGIALPEHDKIDFVKGNVKARMRMVAQYDIAGLVGGLVVGTDHSAENITGFYTKW
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLADYLGAPEVLVSKAPTADLEEEHPQQEDEEALGLTYEQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCCCHHHHCCCHHHH
DDFLEGKVVSNFVNDKLISMYLATQHKREPIPTIYD
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA