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The map label for this gene is holB [H]

Identifier: 167624299

GI number: 167624299

Start: 2875673

End: 2876587

Strand: Reverse

Name: holB [H]

Synonym: Shal_2375

Alternate gene names: 167624299

Gene position: 2876587-2875673 (Counterclockwise)

Preceding gene: 167624300

Following gene: 167624298

Centisome position: 55.03

GC content: 44.7

Gene sequence:

>915_bases
ATGACGCTGCTCCCTTGGCTGCAACATACAATTGAAACCTTTTCAGAGCAACTGAAAACGGGGCGAGTCGGTCATGCTTA
CCTAATTGCGATGGATGTGGCTTACGGTGGTGAAGCCCTCACGCTTGAGCTCGCTAAGGCGGCATTATGTAAACAAGTTG
GCGTTACAGGGAGCTGTGGATTTTGTAAGCCTTGCCAACTTGTTGAGGCAAATAACCACCCTGATCTGTACCGAGTTGAA
GCCGATGGCGCACAGATTAAGGTGGATCAAATTCGTGCGCTGTGCCAAAAATTAACCACAACACCACAACAGGGCGGAAG
ACGCGTTGCGGTGATTTTAAACAGTGAACGTTTGAATCAGGCATCAGCGAATGCATTGCTTAAAACGCTAGAGGAGCCGG
GTAAAGACACCATTTTGTTTTTACAAGCCAATTCACCAACTCGCTTACTGCCAACCATTAGTAGTCGTTGTCAAAGACTT
CATTTAGAAATCCCAAGCAAAGCCGTTGTTAAGCAATGGCTGCAGCAAGAGTTGGCATCGAATGAAGATTTGACTTGGTG
CTTACCTGTAGTCGGTGGGCCGTTAGCTATCCTTTCTGCGGTTGAGAGTGGGCGGGATAAGGCATTGGTAGCGTATAGAA
AAGGCTGGATACAAAGTTTGTCGTCAGGGCACCTGTGTGATAGTTTATTAAATGTTAATGAAAAACAAGTTTCTGATGCG
CTTAAAGTTTTATACCTAGTGGTGCATCGAATATTAGTGAAAAAGCAAGAGATGGATCCTATGCTTAGGGCTAAGCTGGC
TAATTTTGCCGCAAAGGTGATGCTATTAGAAACACGCTTGTCGGTGATGCCAAACGTTAATTCGTTAGCATTATTTGAAG
GATTAACTATTGAGTACAATCAGCTAATGAGCTAA

Upstream 100 bases:

>100_bases
GCGGATAGCGACGACACGATAAAAATTGTCGACGCCAGCCAAAGTATGGCGCAGGTACATAAAGACATTATTGCCATTCT
TCAAGAGCAGAGTTGGTAAT

Downstream 100 bases:

>100_bases
TAAAAATCTATGATGAATCTTGTTGTTAAATTTGAGTCTTTACAGCAGCTTTACCGCGCTTATATGCCGTTTATCAAACC
GATAGGTATGTTTGTTGCTA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTLLPWLQHTIETFSEQLKTGRVGHAYLIAMDVAYGGEALTLELAKAALCKQVGVTGSCGFCKPCQLVEANNHPDLYRVE
ADGAQIKVDQIRALCQKLTTTPQQGGRRVAVILNSERLNQASANALLKTLEEPGKDTILFLQANSPTRLLPTISSRCQRL
HLEIPSKAVVKQWLQQELASNEDLTWCLPVVGGPLAILSAVESGRDKALVAYRKGWIQSLSSGHLCDSLLNVNEKQVSDA
LKVLYLVVHRILVKKQEMDPMLRAKLANFAAKVMLLETRLSVMPNVNSLALFEGLTIEYNQLMS

Sequences:

>Translated_304_residues
MTLLPWLQHTIETFSEQLKTGRVGHAYLIAMDVAYGGEALTLELAKAALCKQVGVTGSCGFCKPCQLVEANNHPDLYRVE
ADGAQIKVDQIRALCQKLTTTPQQGGRRVAVILNSERLNQASANALLKTLEEPGKDTILFLQANSPTRLLPTISSRCQRL
HLEIPSKAVVKQWLQQELASNEDLTWCLPVVGGPLAILSAVESGRDKALVAYRKGWIQSLSSGHLCDSLLNVNEKQVSDA
LKVLYLVVHRILVKKQEMDPMLRAKLANFAAKVMLLETRLSVMPNVNSLALFEGLTIEYNQLMS
>Mature_303_residues
TLLPWLQHTIETFSEQLKTGRVGHAYLIAMDVAYGGEALTLELAKAALCKQVGVTGSCGFCKPCQLVEANNHPDLYRVEA
DGAQIKVDQIRALCQKLTTTPQQGGRRVAVILNSERLNQASANALLKTLEEPGKDTILFLQANSPTRLLPTISSRCQRLH
LEIPSKAVVKQWLQQELASNEDLTWCLPVVGGPLAILSAVESGRDKALVAYRKGWIQSLSSGHLCDSLLNVNEKQVSDAL
KVLYLVVHRILVKKQEMDPMLRAKLANFAAKVMLLETRLSVMPNVNSLALFEGLTIEYNQLMS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=279, Percent_Identity=32.258064516129, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786676, Length=157, Percent_Identity=33.7579617834395, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33498; Mature: 33366

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLPWLQHTIETFSEQLKTGRVGHAYLIAMDVAYGGEALTLELAKAALCKQVGVTGSCG
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCC
FCKPCQLVEANNHPDLYRVEADGAQIKVDQIRALCQKLTTTPQQGGRRVAVILNSERLNQ
CCCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCHHCCH
ASANALLKTLEEPGKDTILFLQANSPTRLLPTISSRCQRLHLEIPSKAVVKQWLQQELAS
HHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHEEHHCCCCHHHHHHHHHHHHCC
NEDLTWCLPVVGGPLAILSAVESGRDKALVAYRKGWIQSLSSGHLCDSLLNVNEKQVSDA
CCCCEEEEHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
LKVLYLVVHRILVKKQEMDPMLRAKLANFAAKVMLLETRLSVMPNVNSLALFEGLTIEYN
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHH
QLMS
HHCC
>Mature Secondary Structure 
TLLPWLQHTIETFSEQLKTGRVGHAYLIAMDVAYGGEALTLELAKAALCKQVGVTGSCG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCC
FCKPCQLVEANNHPDLYRVEADGAQIKVDQIRALCQKLTTTPQQGGRRVAVILNSERLNQ
CCCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCHHCCH
ASANALLKTLEEPGKDTILFLQANSPTRLLPTISSRCQRLHLEIPSKAVVKQWLQQELAS
HHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHEEHHCCCCHHHHHHHHHHHHCC
NEDLTWCLPVVGGPLAILSAVESGRDKALVAYRKGWIQSLSSGHLCDSLLNVNEKQVSDA
CCCCEEEEHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
LKVLYLVVHRILVKKQEMDPMLRAKLANFAAKVMLLETRLSVMPNVNSLALFEGLTIEYN
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHH
QLMS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]