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The map label for this gene is ptsI [H]

Identifier: 167624231

GI number: 167624231

Start: 2791867

End: 2793570

Strand: Direct

Name: ptsI [H]

Synonym: Shal_2307

Alternate gene names: 167624231

Gene position: 2791867-2793570 (Clockwise)

Preceding gene: 167624230

Following gene: 167624232

Centisome position: 53.41

GC content: 42.78

Gene sequence:

>1704_bases
ATGTCGATTACGGGGATCGCTGTTTCATCGGGTATCGCTTTTGGCCAAGCGTTACATCTCAATTTACATCACGAAAAGAT
TGATTACCGTTTAATTCCTAAATCCCATGTCCCCATCGAAGTACAAAGACTAAAGCACGCTTTTGAACTTCAAAAACAGC
ACTTAGCTCAAGGGCTTGAGAAGTTGCAGCCAGAAACCAGCCACTTTGAGTTAATTGAAGCCGATCTGCTCATATTGGAC
GATGAGGATCTGTTAGAACAGATTGAACATAGCATCTCGAGTCTGCAGATCGGCGCTGCATTTGCGATAGAGCGGGTATT
CGAGCAGCAAGCCTCAGAGCTGGCGTTATTAGATGATCCCTATCTAGCCAATCGAGCTCAAGACGTGTCATGTCTCGGTA
GCCGGCTAATCGCCCGTTTAAACGGCGCGCAACATCTCGACTTAAGTCAACTCACACAAGATACAATAATATTTTCTCAA
GACTTAACTCCTGCCGAATTTGCCATGCTACCGCTTGAACATATTACCGGTGTAGTACTCAATACGGGGGGAATAACAAG
CCATACTGCAATTTTGACCCGTAGTGCTGGGATCCCTGCACTGCTAAGTTGTCAATTTGAAGCCATCCAGATAAAAAACG
GTACCCAAGTTGTTTTAAATGCCAATGAAGGTGAGTTGGTTATTAACCCTAACCAACAACAGATTGCTTATTTACAAACA
TTAAAGAAACAAGATTTTCTGCGTAAACAACAATTACTTACCCTTAAAGACTTACCCACTGAAACCCTTGACGGTCATGG
CATTTCTTTATTAGCTAATGTCGGTAACCTAAACGAGGTTAGTCATTTAACCGATGTAAGCGCCGAAGGGATTGGCTTAT
TTAGAACTGAATTCATGTTAATGAATACCAGTGTTTTGCCCGATGAAAAAACTCAATATCATTTATATTGCGATGCCCTG
CATTTAATGGAAGGTAGAACGTTTACCCTACGAACATTAGATGTCGGCGCAGATAAAGAGCTTCCTTGCCTTGGAACATT
AATTGAAGATAATCCGGCGCTAGGCTTAAGGGGGATCCGATATAGCTTGGCCCATCCAGAACTGTTCAAGACACAAGTAA
GTGCTATTTTACGGGCAGCCAATCACGGGTCTGTACGCTTAATGTTTCCGATGATCAATCAAATTGAAGAGTTAGAGCAA
TTGCTAAGCTACGTAGAAGAGTGTAAAGCGAAGCTAATTGAAGAGGAGAAAGGCTTTGGTGAGCTTAGCTATGGCATCGT
TGTCGAAACGCCTGCAGCCGTACTTAACCTCGAGTCGATGCTACCTCTGCTCGATTTTATCAGTATCGGCACAAACGATC
TCACTCAATACACTATGGCAGCGGACAGAAGCAATCCATTACTGACCAAAGAGTATCCCTCTCTCTCCCCTGCGGTGCTC
AATCTTATCCAAACTACCATAGATATCGCCAAGGAAAATCACATCACAGTTTCTCTATGTGGTGAATTAGCAAGCGATCC
AAATGTTGCGCCTTTGCTGGTCGGGATGGGGATAGACGAGCTCAGCGTTAATTTAGGTTCAATATTAGAGGTTAAGTCGG
CATTACGTGATATAAGTTATCAAACATGCTTGACGCTAGCCAAACAAGCGCTAAAAATAAAAAGAATCGAAGAATTAAAA
CGTTACATTTATAGTCGACCTTAA

Upstream 100 bases:

>100_bases
TTATGGCGTATCGGTCAAAGTCTTCGCTGAAGGTGAGCAAGCACAACAAGCTGTTGAAGAAGTGTCAGAACTACTCATTA
CATTAAGTTAAGGGTGTCCT

Downstream 100 bases:

>100_bases
AAGCTATAATAGTGACATTAGCCTTATTCAATATAAAAGATGATTCCAAGGGGCAAATTAGATGGGATTTTTGAGCCGTA
TTAGACGCCTTATTTCAGGA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSITGIAVSSGIAFGQALHLNLHHEKIDYRLIPKSHVPIEVQRLKHAFELQKQHLAQGLEKLQPETSHFELIEADLLILD
DEDLLEQIEHSISSLQIGAAFAIERVFEQQASELALLDDPYLANRAQDVSCLGSRLIARLNGAQHLDLSQLTQDTIIFSQ
DLTPAEFAMLPLEHITGVVLNTGGITSHTAILTRSAGIPALLSCQFEAIQIKNGTQVVLNANEGELVINPNQQQIAYLQT
LKKQDFLRKQQLLTLKDLPTETLDGHGISLLANVGNLNEVSHLTDVSAEGIGLFRTEFMLMNTSVLPDEKTQYHLYCDAL
HLMEGRTFTLRTLDVGADKELPCLGTLIEDNPALGLRGIRYSLAHPELFKTQVSAILRAANHGSVRLMFPMINQIEELEQ
LLSYVEECKAKLIEEEKGFGELSYGIVVETPAAVLNLESMLPLLDFISIGTNDLTQYTMAADRSNPLLTKEYPSLSPAVL
NLIQTTIDIAKENHITVSLCGELASDPNVAPLLVGMGIDELSVNLGSILEVKSALRDISYQTCLTLAKQALKIKRIEELK
RYIYSRP

Sequences:

>Translated_567_residues
MSITGIAVSSGIAFGQALHLNLHHEKIDYRLIPKSHVPIEVQRLKHAFELQKQHLAQGLEKLQPETSHFELIEADLLILD
DEDLLEQIEHSISSLQIGAAFAIERVFEQQASELALLDDPYLANRAQDVSCLGSRLIARLNGAQHLDLSQLTQDTIIFSQ
DLTPAEFAMLPLEHITGVVLNTGGITSHTAILTRSAGIPALLSCQFEAIQIKNGTQVVLNANEGELVINPNQQQIAYLQT
LKKQDFLRKQQLLTLKDLPTETLDGHGISLLANVGNLNEVSHLTDVSAEGIGLFRTEFMLMNTSVLPDEKTQYHLYCDAL
HLMEGRTFTLRTLDVGADKELPCLGTLIEDNPALGLRGIRYSLAHPELFKTQVSAILRAANHGSVRLMFPMINQIEELEQ
LLSYVEECKAKLIEEEKGFGELSYGIVVETPAAVLNLESMLPLLDFISIGTNDLTQYTMAADRSNPLLTKEYPSLSPAVL
NLIQTTIDIAKENHITVSLCGELASDPNVAPLLVGMGIDELSVNLGSILEVKSALRDISYQTCLTLAKQALKIKRIEELK
RYIYSRP
>Mature_566_residues
SITGIAVSSGIAFGQALHLNLHHEKIDYRLIPKSHVPIEVQRLKHAFELQKQHLAQGLEKLQPETSHFELIEADLLILDD
EDLLEQIEHSISSLQIGAAFAIERVFEQQASELALLDDPYLANRAQDVSCLGSRLIARLNGAQHLDLSQLTQDTIIFSQD
LTPAEFAMLPLEHITGVVLNTGGITSHTAILTRSAGIPALLSCQFEAIQIKNGTQVVLNANEGELVINPNQQQIAYLQTL
KKQDFLRKQQLLTLKDLPTETLDGHGISLLANVGNLNEVSHLTDVSAEGIGLFRTEFMLMNTSVLPDEKTQYHLYCDALH
LMEGRTFTLRTLDVGADKELPCLGTLIEDNPALGLRGIRYSLAHPELFKTQVSAILRAANHGSVRLMFPMINQIEELEQL
LSYVEECKAKLIEEEKGFGELSYGIVVETPAAVLNLESMLPLLDFISIGTNDLTQYTMAADRSNPLLTKEYPSLSPAVLN
LIQTTIDIAKENHITVSLCGELASDPNVAPLLVGMGIDELSVNLGSILEVKSALRDISYQTCLTLAKQALKIKRIEELKR
YIYSRP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=563, Percent_Identity=38.3658969804618, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI48994992, Length=487, Percent_Identity=34.7022587268994, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1788726, Length=566, Percent_Identity=31.8021201413428, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789193, Length=465, Percent_Identity=33.9784946236559, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1787994, Length=429, Percent_Identity=25.4079254079254, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62655; Mature: 62524

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITGIAVSSGIAFGQALHLNLHHEKIDYRLIPKSHVPIEVQRLKHAFELQKQHLAQGLE
CCEEEEEEECCCCCCEEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KLQPETSHFELIEADLLILDDEDLLEQIEHSISSLQIGAAFAIERVFEQQASELALLDDP
HHCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCC
YLANRAQDVSCLGSRLIARLNGAQHLDLSQLTQDTIIFSQDLTPAEFAMLPLEHITGVVL
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEECCCCHHHHHHCCHHHHCEEEE
NTGGITSHTAILTRSAGIPALLSCQFEAIQIKNGTQVVLNANEGELVINPNQQQIAYLQT
ECCCCCCCCEEEEECCCCCEEEECCEEEEEECCCCEEEEECCCCEEEECCCHHHHHHHHH
LKKQDFLRKQQLLTLKDLPTETLDGHGISLLANVGNLNEVSHLTDVSAEGIGLFRTEFML
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHEEEE
MNTSVLPDEKTQYHLYCDALHLMEGRTFTLRTLDVGADKELPCLGTLIEDNPALGLRGIR
EECCCCCCCCCEEEEEEHHHHHHCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCE
YSLAHPELFKTQVSAILRAANHGSVRLMFPMINQIEELEQLLSYVEECKAKLIEEEKGFG
EECCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELSYGIVVETPAAVLNLESMLPLLDFISIGTNDLTQYTMAADRSNPLLTKEYPSLSPAVL
CEECCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEECCCCCCCHHHH
NLIQTTIDIAKENHITVSLCGELASDPNVAPLLVGMGIDELSVNLGSILEVKSALRDISY
HHHHHHHHHHCCCCEEEEEECHHCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHCCH
QTCLTLAKQALKIKRIEELKRYIYSRP
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SITGIAVSSGIAFGQALHLNLHHEKIDYRLIPKSHVPIEVQRLKHAFELQKQHLAQGLE
CEEEEEEECCCCCCEEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KLQPETSHFELIEADLLILDDEDLLEQIEHSISSLQIGAAFAIERVFEQQASELALLDDP
HHCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCC
YLANRAQDVSCLGSRLIARLNGAQHLDLSQLTQDTIIFSQDLTPAEFAMLPLEHITGVVL
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEECCCCHHHHHHCCHHHHCEEEE
NTGGITSHTAILTRSAGIPALLSCQFEAIQIKNGTQVVLNANEGELVINPNQQQIAYLQT
ECCCCCCCCEEEEECCCCCEEEECCEEEEEECCCCEEEEECCCCEEEECCCHHHHHHHHH
LKKQDFLRKQQLLTLKDLPTETLDGHGISLLANVGNLNEVSHLTDVSAEGIGLFRTEFML
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHEEEE
MNTSVLPDEKTQYHLYCDALHLMEGRTFTLRTLDVGADKELPCLGTLIEDNPALGLRGIR
EECCCCCCCCCEEEEEEHHHHHHCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCE
YSLAHPELFKTQVSAILRAANHGSVRLMFPMINQIEELEQLLSYVEECKAKLIEEEKGFG
EECCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELSYGIVVETPAAVLNLESMLPLLDFISIGTNDLTQYTMAADRSNPLLTKEYPSLSPAVL
CEECCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEECCCCCCCHHHH
NLIQTTIDIAKENHITVSLCGELASDPNVAPLLVGMGIDELSVNLGSILEVKSALRDISY
HHHHHHHHHHCCCCEEEEEECHHCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHCCH
QTCLTLAKQALKIKRIEELKRYIYSRP
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]