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The map label for this gene is adhE [H]

Identifier: 167624174

GI number: 167624174

Start: 2725691

End: 2728306

Strand: Direct

Name: adhE [H]

Synonym: Shal_2250

Alternate gene names: 167624174

Gene position: 2725691-2728306 (Clockwise)

Preceding gene: 167624172

Following gene: 167624177

Centisome position: 52.15

GC content: 46.41

Gene sequence:

>2616_bases
ATGACAGTATCAAATGAGCAAGAACTCAATCTCCTAGTAGACAGAGTGCGAACGGCGCAACAGACGTTCGCTAACTACTC
TCAGGAACAAGTCGATAAGATTTTCAGAGCCGCAGCCCTCGCCGCTGCCGATGCGCGGATCTCACTTGCTAAGATGGCCG
CCAATGAAACTAAAATGGGCGTCATCGAAGATAAGGTGATTAAAAATCACTTTGCCTCAGAATATATCTACAACAAATAT
AAAGATGAAAAAACCTGCGGGATCCTATCTGAAGATCCCACATTTGGAACCATCACCATCGCCGAGCCTGTCGGCATTAT
CTGTGGCATAGTGCCAACCACTAACCCTACATCTACAGCAATCTTTAAGGCTCTGATTAGCCTTAAGACTCGAAACGGTA
TTATTTTCTCCCCCCACCCAAGAGCTAAAGAGTCGACGACAACTGCCGCTCGTATCGTATTGGAAGCAGCCGTTGCTGCA
GGAGCCCCAAAAGACATTATTGGCTGGATAGATGAGCCATCAGTCGCATTATCCAATCAATTAATGACCCACAAAGATAT
TAATCTCATTCTTGCTACTGGCGGGCCGGGAATGGTAAAAGCCGCCTACTCTTCAGGTAAACCAGCTATTGGCGTCGGCG
CAGGTAATACACCAATTGTTATTGATGAAAGCGCAGATATAAAACGCGCCGTTAGCTCTATATTGATGTCAAAAACCTTC
GATAACGGCGTTGTCTGTGCATCTGAACAAGCTGTTGTTATCGTAGATGAGGTTTACGAGGCGGTTAAAGCGCGTTTTGC
AAGCCACGGCGGCTACATTTTAAGCCCTGACGAAACTGCTGCAATGCAAAATGTTATTTTAAAAAATGGCGGGCTTAATG
CCGATATCGTCGGTCAAAGCGCCGCAAGCATTGCCAAGATGGCCGGAATAGAGGTACACCACACAACTAAAGTGCTGATC
GGTGAGGTGTCCGATATTGATGAAGCCGAAGCCTTCGCCCATGAAAAACTCTCACCGTTGCTGGCTATGTACCGCGCAGC
CGATTTTGACGATGCTGTAGATAAAGCTGAAGCCCTAGTCACGCTGGGTGGTATTGGACATACATCGGGGCTGTATACGG
ATCAGGATACTCAAATTGAAAGAGTTAAAGCCTTCGGCTTTCGAATGAAGACCGCGCGTATTCTCATTAACACTCCGGCT
TCACAGGGCGGGATTGGCGATCTCTATAACTTCAAGCTCGCTCCGTCGCTAACCTTAGGTTGTGGCTCTTGGGGTGGCAA
CTCCATTTCAGAAAATGTCGGTCCGAGCCATTTGATCAATAAGAAAATGGTCGCTAAGAGAGCTGAAAATATGTTGTGGC
ACAAACTTCCCTCTTCAATTTATTTCCGTCGTGGTAGCTTACCTATCGCACTAGAGGAGCTCAGCGATAAGAAGCGTGCG
CTTATCGTGACTGACAAGTATCTATTTAATAATGGTTACTGCGATGAAACCATCAAGATCTTAAAGAGCCAAGGTCTTGA
AACCGAAGTTTTCTATGAAGTAGAAGCCGACCCAACGATGCAAATCGTCAAGCAAGGTGCCAAAGTTGCGCAAAGCTTCC
AACCTGACGTTATTATCGCCTTAGGCGGCGGCTCGCCAATGGACGCCGCCAAGATCATCTGGGTGATGTATGAACATCCT
GATGTCGATTTCGCCGATTTAGCGCTACGCTTTATGGATATCAGAAAACGTATCTATAAATTCCCAAAACTGGGTAGAAA
AGCCAAGATGGTCGCGATACCAACCACATCTGGTACTGGCTCAGAAGTGACTCCATTTGCAGTCGTTACCGATGAACAAA
CGGGTATGAAGTACCCCATTGCCGATTATGAGCTAACGCCAAACATGGCGATTGTTGACCCTAATTTAGTCATGAACATG
CCAAAGTCACTCACAGCCTTTGGTGGCATAGACGCTGTGACCCATGCATTAGAAGCTTATGTGAGCGTAATGGCCAACGA
ATATAGCGATGGCCAAGCATTACAAGCACTAGACTTACTGTTTAAGCATCTTCCAGACGCCTACGAACATGGCGCCAATG
CGCCAGTGGCTCGTGAAAAAGTACATAACGGTGCAACCATTGCAGGCATCGCTTTTGCCAATGCTTTCTTGGGTATTTGC
CACTCTATGGCTCACAAACTGGGTGCAGAGTTCCATTTAGCCCATGGTTTAGCGAATGCGTTACTTATCAGCAATGTGAT
CCGTTTTAACGCAACCGATATGCCCACTAAACAGGCGGCATTTAGCCAATACGATAGACCGAAAGCACTTTGTCGATATG
CAAAGATTGCTGAATATTTAAAACTGGAAGGCGCCATAGGCGAAGGCAAAACTGATGCGGAAAAAGTCGAGACACTGCTT
AAAAAAATTGATGAACTCAAAGAGACGATTGGCATTCCACGCTCGATTCAAGCCGCAGGCGTCAACGAAGCCGATTTCCT
CGCAAAACTCGATGAGTTAGCAGAAGATGCCTTTGATGATCAGTGTACAGGCGCCAATCCTAGATACCCATTAATTGCAG
AGATAAAACAGATCCTTTTAGCCAGCTACTATGGAGAAAGCTATCGCGATCAGTAA

Upstream 100 bases:

>100_bases
CTCGCCTTTGCGTGTAATTTTTCTTCATCAAATGGATTTTAGAGCACCGGAGAATGTTTAGCCAAATACCGTTTGGCTAA
ATTTTTTCAGGATTGCGATT

Downstream 100 bases:

>100_bases
ACCATTTTAGTCATTAAAAAGCCCTAGCCTTCGGTTAGGGCTTTTTGCTGTGCCTATTACTATCACCCGTAACACATAAA
GCGACTAAGACTGAAGCTTG

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MTVSNEQELNLLVDRVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAANETKMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKESTTTAARIVLEAAVAA
GAPKDIIGWIDEPSVALSNQLMTHKDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDESADIKRAVSSILMSKTF
DNGVVCASEQAVVIVDEVYEAVKARFASHGGYILSPDETAAMQNVILKNGGLNADIVGQSAASIAKMAGIEVHHTTKVLI
GEVSDIDEAEAFAHEKLSPLLAMYRAADFDDAVDKAEALVTLGGIGHTSGLYTDQDTQIERVKAFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDKYLFNNGYCDETIKILKSQGLETEVFYEVEADPTMQIVKQGAKVAQSFQPDVIIALGGGSPMDAAKIIWVMYEHP
DVDFADLALRFMDIRKRIYKFPKLGRKAKMVAIPTTSGTGSEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMNM
PKSLTAFGGIDAVTHALEAYVSVMANEYSDGQALQALDLLFKHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDMPTKQAAFSQYDRPKALCRYAKIAEYLKLEGAIGEGKTDAEKVETLL
KKIDELKETIGIPRSIQAAGVNEADFLAKLDELAEDAFDDQCTGANPRYPLIAEIKQILLASYYGESYRDQ

Sequences:

>Translated_871_residues
MTVSNEQELNLLVDRVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAANETKMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKESTTTAARIVLEAAVAA
GAPKDIIGWIDEPSVALSNQLMTHKDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDESADIKRAVSSILMSKTF
DNGVVCASEQAVVIVDEVYEAVKARFASHGGYILSPDETAAMQNVILKNGGLNADIVGQSAASIAKMAGIEVHHTTKVLI
GEVSDIDEAEAFAHEKLSPLLAMYRAADFDDAVDKAEALVTLGGIGHTSGLYTDQDTQIERVKAFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDKYLFNNGYCDETIKILKSQGLETEVFYEVEADPTMQIVKQGAKVAQSFQPDVIIALGGGSPMDAAKIIWVMYEHP
DVDFADLALRFMDIRKRIYKFPKLGRKAKMVAIPTTSGTGSEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMNM
PKSLTAFGGIDAVTHALEAYVSVMANEYSDGQALQALDLLFKHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDMPTKQAAFSQYDRPKALCRYAKIAEYLKLEGAIGEGKTDAEKVETLL
KKIDELKETIGIPRSIQAAGVNEADFLAKLDELAEDAFDDQCTGANPRYPLIAEIKQILLASYYGESYRDQ
>Mature_870_residues
TVSNEQELNLLVDRVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAANETKMGVIEDKVIKNHFASEYIYNKYK
DEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKESTTTAARIVLEAAVAAG
APKDIIGWIDEPSVALSNQLMTHKDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDESADIKRAVSSILMSKTFD
NGVVCASEQAVVIVDEVYEAVKARFASHGGYILSPDETAAMQNVILKNGGLNADIVGQSAASIAKMAGIEVHHTTKVLIG
EVSDIDEAEAFAHEKLSPLLAMYRAADFDDAVDKAEALVTLGGIGHTSGLYTDQDTQIERVKAFGFRMKTARILINTPAS
QGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRAL
IVTDKYLFNNGYCDETIKILKSQGLETEVFYEVEADPTMQIVKQGAKVAQSFQPDVIIALGGGSPMDAAKIIWVMYEHPD
VDFADLALRFMDIRKRIYKFPKLGRKAKMVAIPTTSGTGSEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMNMP
KSLTAFGGIDAVTHALEAYVSVMANEYSDGQALQALDLLFKHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGICH
SMAHKLGAEFHLAHGLANALLISNVIRFNATDMPTKQAAFSQYDRPKALCRYAKIAEYLKLEGAIGEGKTDAEKVETLLK
KIDELKETIGIPRSIQAAGVNEADFLAKLDELAEDAFDDQCTGANPRYPLIAEIKQILLASYYGESYRDQ

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=320, Percent_Identity=27.5, Blast_Score=86, Evalue=2e-16,
Organism=Escherichia coli, GI1787493, Length=870, Percent_Identity=75.5172413793103, Blast_Score=1338, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=413, Percent_Identity=36.0774818401937, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1789163, Length=410, Percent_Identity=31.7073170731707, Blast_Score=194, Evalue=3e-50,
Organism=Escherichia coli, GI87082107, Length=384, Percent_Identity=30.9895833333333, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1788797, Length=372, Percent_Identity=31.1827956989247, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789386, Length=393, Percent_Identity=24.4274809160305, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=282, Percent_Identity=27.6595744680851, Blast_Score=110, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=396, Percent_Identity=32.5757575757576, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24657991, Length=319, Percent_Identity=26.3322884012539, Blast_Score=90, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94112; Mature: 93981

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSNEQELNLLVDRVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAANETKMG
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VIEDKVIKNHFASEYIYNKYKDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNGIIFSPHPRAKESTTTAARIVLEAAVAAGAPKDIIGWIDEPSVALSNQ
HHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMTHKDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDESADIKRAVSSILMSKTF
HHEECCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHCC
DNGVVCASEQAVVIVDEVYEAVKARFASHGGYILSPDETAAMQNVILKNGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCEECCCH
AASIAKMAGIEVHHTTKVLIGEVSDIDEAEAFAHEKLSPLLAMYRAADFDDAVDKAEALV
HHHHHHHHCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHE
TLGGIGHTSGLYTDQDTQIERVKAFGFRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
EECCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCCE
LIVTDKYLFNNGYCDETIKILKSQGLETEVFYEVEADPTMQIVKQGAKVAQSFQPDVIIA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
LGGGSPMDAAKIIWVMYEHPDVDFADLALRFMDIRKRIYKFPKLGRKAKMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMNMPKSLTAFGGIDAVTHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEECCHHHHCCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLFKHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDMPTKQAAFSQYDRPKALCRYAKIAEYL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
KLEGAIGEGKTDAEKVETLLKKIDELKETIGIPRSIQAAGVNEADFLAKLDELAEDAFDD
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHCCC
QCTGANPRYPLIAEIKQILLASYYGESYRDQ
CCCCCCCCCCHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
TVSNEQELNLLVDRVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAANETKMG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VIEDKVIKNHFASEYIYNKYKDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNGIIFSPHPRAKESTTTAARIVLEAAVAAGAPKDIIGWIDEPSVALSNQ
HHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMTHKDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDESADIKRAVSSILMSKTF
HHEECCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHCC
DNGVVCASEQAVVIVDEVYEAVKARFASHGGYILSPDETAAMQNVILKNGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCEECCCH
AASIAKMAGIEVHHTTKVLIGEVSDIDEAEAFAHEKLSPLLAMYRAADFDDAVDKAEALV
HHHHHHHHCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHE
TLGGIGHTSGLYTDQDTQIERVKAFGFRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
EECCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCCE
LIVTDKYLFNNGYCDETIKILKSQGLETEVFYEVEADPTMQIVKQGAKVAQSFQPDVIIA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
LGGGSPMDAAKIIWVMYEHPDVDFADLALRFMDIRKRIYKFPKLGRKAKMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMNMPKSLTAFGGIDAVTHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEECCHHHHCCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLFKHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDMPTKQAAFSQYDRPKALCRYAKIAEYL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
KLEGAIGEGKTDAEKVETLLKKIDELKETIGIPRSIQAAGVNEADFLAKLDELAEDAFDD
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHCCC
QCTGANPRYPLIAEIKQILLASYYGESYRDQ
CCCCCCCCCCHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]