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The map label for this gene is bepF [H]

Identifier: 167624090

GI number: 167624090

Start: 2615260

End: 2616486

Strand: Direct

Name: bepF [H]

Synonym: Shal_2166

Alternate gene names: 167624090

Gene position: 2615260-2616486 (Clockwise)

Preceding gene: 167624086

Following gene: 167624091

Centisome position: 50.03

GC content: 44.17

Gene sequence:

>1227_bases
ATGCCAAGTCATCTCAGCAGCACAGCATTCAAATTAACACTGCTTTTTTCATCCGTGGCATTCATTACTGGCTGTGAAAA
CGAGCCTGTGAAAATCACCCCTAAATTAGTCGTTGTTGAAAGAGTATCGACAGCAACAGTGCCGCTCTATGGCAATTATG
TCGGTGTAACAAAGGCCTCGTTGGATGTTGAAGTAAGAGCGAGAGTCGATGGCTTTGTTGAAAATAAAACGTTTATTGAA
GGTAGTGCGGTTAAAGAAGGGGTGCTACTTTATCGCATCGATAATCGTCCTTATGTGGCAGTGGTTAACCGTTTAAGTGC
TAATGTTGAATCCCAGCAATCAGCGCTCGAAAAGGCACAACGTGATGTCGAACGTTTAAAGCCGTTGTATGAGCAAGATG
CAGCCAGTCAGTTAGATTTCGATAATGCGCTGTCGGTTCTGTCGCAAGCAAAATCCAGTGTCGCCGCAAGTAAAGCCGAG
CTTGAAGAAGCAAAACTTGAACTCAGTTATACCGAGATTAAGTCACCCATTAGTGGATTAGTCAGCCGCTCAGAAGTTGA
TATCGGTGCGTTAGTCGGCAGCAGTGGCCAATCGTTATTAACTCGGGTTAAGCAGGTCGATCCTATCTATGTCACCTTTA
ATATGTCGGCGTTAGATTATCTTAATGCGCGTCGCCGCATGACCAGTGATAAAGAGCAACAAGCCGCTGAATCAGAAGGT
AAAGCTGTTGAAGGTTTCGTAACAATTACATTGCCAGATAACAGTGAGTACCGGTATTTGGGTGATGTCGGCTTTACCGA
TCCTTCTGTCAATCCTGAAACAGGTACTTTTCAGGTAAGAGCTGAACTACCTAACCCCGATAAAGAGTTACTGCCGGGCC
AATATACCAATGTCAGAATTAAGCTCAGTGAAGTGAGTGATGCAATCGTTATCCCACAAAAAGCCACTCAAGTTGAGCAA
GGTGGCGTCTATGTGATGATTGTGCTGCCCAATAATAAAGTTGAACGCCGCTTTATTGTGATAAAACATCAAGGTGAAAT
GGGCGTAGTAGTACAAAGTGGTTTAAAAGCGGGAGAATTAGTCATTGTCGAAGGCATGCATCGTGTCCGTCATGGACAAC
TGGCTGAGCCGTTAACTGCCGAGCAGTATGATAAAAAAGAGGATGTAAAACAGAAAGAGCAAGCATTGAAGCAACAAAAG
CTTGAGCAGGAACAGGAGGATAACTAA

Upstream 100 bases:

>100_bases
CATCTTCATTACAAGAATAACCAGTGCGAATTCAATTACATCTAATTGTTTTTAACAGCACCATTAGAGATTTGTGAACA
GCACATTTAGGGACGAACGT

Downstream 100 bases:

>100_bases
TGGCACAGTATTTTGTTAATCGTCCCGTTTTTGCCAGTGTAATCTCTATTGTCATTGTGCTATTGGGGTTAATTGCGATG
TTTCAATTACCCATTGACCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPSHLSSTAFKLTLLFSSVAFITGCENEPVKITPKLVVVERVSTATVPLYGNYVGVTKASLDVEVRARVDGFVENKTFIE
GSAVKEGVLLYRIDNRPYVAVVNRLSANVESQQSALEKAQRDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAE
LEEAKLELSYTEIKSPISGLVSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRIKLSEVSDAIVIPQKATQVEQ
GGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGELVIVEGMHRVRHGQLAEPLTAEQYDKKEDVKQKEQALKQQK
LEQEQEDN

Sequences:

>Translated_408_residues
MPSHLSSTAFKLTLLFSSVAFITGCENEPVKITPKLVVVERVSTATVPLYGNYVGVTKASLDVEVRARVDGFVENKTFIE
GSAVKEGVLLYRIDNRPYVAVVNRLSANVESQQSALEKAQRDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAE
LEEAKLELSYTEIKSPISGLVSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRIKLSEVSDAIVIPQKATQVEQ
GGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGELVIVEGMHRVRHGQLAEPLTAEQYDKKEDVKQKEQALKQQK
LEQEQEDN
>Mature_407_residues
PSHLSSTAFKLTLLFSSVAFITGCENEPVKITPKLVVVERVSTATVPLYGNYVGVTKASLDVEVRARVDGFVENKTFIEG
SAVKEGVLLYRIDNRPYVAVVNRLSANVESQQSALEKAQRDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAEL
EEAKLELSYTEIKSPISGLVSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEGK
AVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRIKLSEVSDAIVIPQKATQVEQG
GVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGELVIVEGMHRVRHGQLAEPLTAEQYDKKEDVKQKEQALKQQKL
EQEQEDN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=388, Percent_Identity=31.701030927835, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786668, Length=402, Percent_Identity=32.5870646766169, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789665, Length=365, Percent_Identity=33.1506849315069, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI87082036, Length=337, Percent_Identity=27.5964391691395, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44992; Mature: 44860

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSHLSSTAFKLTLLFSSVAFITGCENEPVKITPKLVVVERVSTATVPLYGNYVGVTKAS
CCCCCCHHHHHHHHHHHHHHHEECCCCCCEEECCEEEEEEECCCEEEEEECCEEEEEEEE
LDVEVRARVDGFVENKTFIEGSAVKEGVLLYRIDNRPYVAVVNRLSANVESQQSALEKAQ
EEEEEEEEECCEECCCEEECCCCCCCCEEEEEECCCCEEEEEHHHHCCHHHHHHHHHHHH
RDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAELEEAKLELSYTEIKSPISGL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHH
VSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
HHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHCC
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRI
CEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEE
KLSEVSDAIVIPQKATQVEQGGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGEL
EEECCCCEEEECCHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCCCCCE
VIVEGMHRVRHGQLAEPLTAEQYDKKEDVKQKEQALKQQKLEQEQEDN
EEEECHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSHLSSTAFKLTLLFSSVAFITGCENEPVKITPKLVVVERVSTATVPLYGNYVGVTKAS
CCCCCHHHHHHHHHHHHHHHEECCCCCCEEECCEEEEEEECCCEEEEEECCEEEEEEEE
LDVEVRARVDGFVENKTFIEGSAVKEGVLLYRIDNRPYVAVVNRLSANVESQQSALEKAQ
EEEEEEEEECCEECCCEEECCCCCCCCEEEEEECCCCEEEEEHHHHCCHHHHHHHHHHHH
RDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAELEEAKLELSYTEIKSPISGL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHH
VSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
HHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHCC
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRI
CEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEE
KLSEVSDAIVIPQKATQVEQGGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGEL
EEECCCCEEEECCHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCCCCCE
VIVEGMHRVRHGQLAEPLTAEQYDKKEDVKQKEQALKQQKLEQEQEDN
EEEECHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]