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The map label for this gene is emrB [H]

Identifier: 167624087

GI number: 167624087

Start: 2612071

End: 2613612

Strand: Reverse

Name: emrB [H]

Synonym: Shal_2163

Alternate gene names: 167624087

Gene position: 2613612-2612071 (Counterclockwise)

Preceding gene: 167624088

Following gene: 167624085

Centisome position: 50.0

GC content: 45.91

Gene sequence:

>1542_bases
ATGAGCGATACTTCAGAGACGATGCCTGATACTGATGACAGTCAGGTAATGAGTGAAAGCAGCCGTAAACTCGTCACTTT
AGCGGTAATGCTATCGGCCATCATGGTATTGCTTGATATGACAATCGCCAATGTTGCCCTGCCTCATATGATGGGCGCAT
TGGGGGTCACATCAGATCAAGTGACCTGGGTACTGACTTCCTATTCTATGGCTGAAGCGATATTTATCCCGCTAGCCAGT
TTTCTCGCGCTTAAATTTGGAGTTCGTAATCTACTGCTGATCTCGGTCAGTGGATTTATGATCAGCTCAGCGCTTTGCGG
CCAAGCAGATAGCATCGCTGAAATGGTCACCTTTAGGGTGATGCAAGGGGCATTTGGTGCCTCAGTTATCCCGCTTTCTC
AGTCGATTATGGTGCAGATCTACCCTGCAAACCAACGCGGTAAAGCCATGGCACTCTTTAGTGTCGGGGTATTACTGGGG
CCAATATTGGGGCCTACGCTTGGCGGGATCATTACCGAGAATATGAATTGGCGCTGGATTTTTTACGTTAACCTGCCTAT
TGGTGCAATCTGCCTTACGCTGATTTACACCTTTGTTAAGCTAGGTGGTAAAGGTAAACCTAAAATTGACTGGCCAATTG
TCATCGCAATGACCATTGGTATTGGTCTGCTACAGATGGTGCTCGACCGAGGTAATCAAGAGTCTTGGTTTGAGTCTAAT
ACCATCTTGTTTTCAGCCATCATCAGCGCTATTGCGATTATTTTCTTTGTTGCGCGCTCTTTCATTACTAAGAGTGAGAT
AGCACCGGTCTGGTTATTACACGATCGTAACCTTGCTATGTCATGCCTTGTGATGGCGGGCTTCTCGATGGGAATGTTTG
GCATCACTCAGCTGCAACCCATGATGCTAGAACAGTTATTGAATTACCCGGTAGAAACTACGGGGTTTGCTATGGCGCCA
AGGGGTCTTGCATCTGCCATTGTTTTACTTGCAATGGCACGTTACATGGACAAAATCGATGCAAGGCTACTTATCGCTGT
AGGTCTGTCACTTAATGCCCTTGGCACGTATTTGATGACTCAATATTCACTTGAAATCGACATCTACTGGATCTTACTAC
CTAGTATTATACAAGGTGCTGGTATGGGATTAGTTTTTGCCCCATTAAGTCAGCTTGCCTATACAACCTTGTCACCAAAA
GACACTATCGGTGGTGCAGTGGTGTTTAATTTATGCCGGACAATTGGTGGCTCTTTCGGCATATCTATTGTGAATACCTA
TTTTTCAAGGATCCAGCAACAAGAGTGGAACCAGTTAGGAGGCTCACTTTCGCTAAGCAACCCCGTGTTACAGAACGCGG
CGCAGATGCAAGGTACCAGCATCACAGACCCTACTTTCTTAGGGCAGCTCCAAAATATGCTGCAGCAACAGTCGACACTC
ACAGCCTTCGTCTATACCTTTGGCTTCCTGTTAATCTCTTACCTCTGTCTGATCCCGCTTCTTGCACTATTCAAAACTAA
GCCAAAAATTGAGACTCAGTAA

Upstream 100 bases:

>100_bases
AATGAAAGTGAGCACCCTGACTTTCCTCTACGTGTTGGCGCAAGTGCTAACGTAAGCATAGACACGGTTAATAGCCCATC
TAAGCAACAAGAGCAGTAAC

Downstream 100 bases:

>100_bases
TGCATATATTTAGCGCTCGATACCGAGCGCTTTCTCGCACTTATAAGCGTTGGCAATCATGGTGACTGAGCCACTGAAGC
CTAGTGGCTTATATAGATAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDTSETMPDTDDSQVMSESSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQVTWVLTSYSMAEAIFIPLAS
FLALKFGVRNLLLISVSGFMISSALCGQADSIAEMVTFRVMQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLG
PILGPTLGGIITENMNWRWIFYVNLPIGAICLTLIYTFVKLGGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
TILFSAIISAIAIIFFVARSFITKSEIAPVWLLHDRNLAMSCLVMAGFSMGMFGITQLQPMMLEQLLNYPVETTGFAMAP
RGLASAIVLLAMARYMDKIDARLLIAVGLSLNALGTYLMTQYSLEIDIYWILLPSIIQGAGMGLVFAPLSQLAYTTLSPK
DTIGGAVVFNLCRTIGGSFGISIVNTYFSRIQQQEWNQLGGSLSLSNPVLQNAAQMQGTSITDPTFLGQLQNMLQQQSTL
TAFVYTFGFLLISYLCLIPLLALFKTKPKIETQ

Sequences:

>Translated_513_residues
MSDTSETMPDTDDSQVMSESSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQVTWVLTSYSMAEAIFIPLAS
FLALKFGVRNLLLISVSGFMISSALCGQADSIAEMVTFRVMQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLG
PILGPTLGGIITENMNWRWIFYVNLPIGAICLTLIYTFVKLGGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
TILFSAIISAIAIIFFVARSFITKSEIAPVWLLHDRNLAMSCLVMAGFSMGMFGITQLQPMMLEQLLNYPVETTGFAMAP
RGLASAIVLLAMARYMDKIDARLLIAVGLSLNALGTYLMTQYSLEIDIYWILLPSIIQGAGMGLVFAPLSQLAYTTLSPK
DTIGGAVVFNLCRTIGGSFGISIVNTYFSRIQQQEWNQLGGSLSLSNPVLQNAAQMQGTSITDPTFLGQLQNMLQQQSTL
TAFVYTFGFLLISYLCLIPLLALFKTKPKIETQ
>Mature_512_residues
SDTSETMPDTDDSQVMSESSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQVTWVLTSYSMAEAIFIPLASF
LALKFGVRNLLLISVSGFMISSALCGQADSIAEMVTFRVMQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLGP
ILGPTLGGIITENMNWRWIFYVNLPIGAICLTLIYTFVKLGGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESNT
ILFSAIISAIAIIFFVARSFITKSEIAPVWLLHDRNLAMSCLVMAGFSMGMFGITQLQPMMLEQLLNYPVETTGFAMAPR
GLASAIVLLAMARYMDKIDARLLIAVGLSLNALGTYLMTQYSLEIDIYWILLPSIIQGAGMGLVFAPLSQLAYTTLSPKD
TIGGAVVFNLCRTIGGSFGISIVNTYFSRIQQQEWNQLGGSLSLSNPVLQNAAQMQGTSITDPTFLGQLQNMLQQQSTLT
AFVYTFGFLLISYLCLIPLLALFKTKPKIETQ

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=459, Percent_Identity=33.7690631808279, Blast_Score=248, Evalue=9e-67,
Organism=Escherichia coli, GI1788710, Length=459, Percent_Identity=33.3333333333333, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=30.2211302211302, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790195, Length=393, Percent_Identity=25.6997455470738, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788392, Length=398, Percent_Identity=26.1306532663317, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=153, Percent_Identity=31.3725490196078, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=30.2631578947368, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323000, Length=123, Percent_Identity=30.0813008130081, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=181, Percent_Identity=25.9668508287293, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=384, Percent_Identity=24.7395833333333, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55783; Mature: 55652

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSETMPDTDDSQVMSESSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VTWVLTSYSMAEAIFIPLASFLALKFGVRNLLLISVSGFMISSALCGQADSIAEMVTFRV
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEHEEEHHHHHHHHHCCCHHHHHHHHHHHH
MQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLGPILGPTLGGIITENMNWRWI
HHHCCCCEEEECCCEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHEECCCCEEEE
FYVNLPIGAICLTLIYTFVKLGGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
TILFSAIISAIAIIFFVARSFITKSEIAPVWLLHDRNLAMSCLVMAGFSMGMFGITQLQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCHHHHHHCCH
MMLEQLLNYPVETTGFAMAPRGLASAIVLLAMARYMDKIDARLLIAVGLSLNALGTYLMT
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QYSLEIDIYWILLPSIIQGAGMGLVFAPLSQLAYTTLSPKDTIGGAVVFNLCRTIGGSFG
EEEEEEEEHHEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCC
ISIVNTYFSRIQQQEWNQLGGSLSLSNPVLQNAAQMQGTSITDPTFLGQLQNMLQQQSTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TAFVYTFGFLLISYLCLIPLLALFKTKPKIETQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDTSETMPDTDDSQVMSESSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VTWVLTSYSMAEAIFIPLASFLALKFGVRNLLLISVSGFMISSALCGQADSIAEMVTFRV
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEHEEEHHHHHHHHHCCCHHHHHHHHHHHH
MQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLGPILGPTLGGIITENMNWRWI
HHHCCCCEEEECCCEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHEECCCCEEEE
FYVNLPIGAICLTLIYTFVKLGGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
TILFSAIISAIAIIFFVARSFITKSEIAPVWLLHDRNLAMSCLVMAGFSMGMFGITQLQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCHHHHHHCCH
MMLEQLLNYPVETTGFAMAPRGLASAIVLLAMARYMDKIDARLLIAVGLSLNALGTYLMT
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QYSLEIDIYWILLPSIIQGAGMGLVFAPLSQLAYTTLSPKDTIGGAVVFNLCRTIGGSFG
EEEEEEEEHHEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCC
ISIVNTYFSRIQQQEWNQLGGSLSLSNPVLQNAAQMQGTSITDPTFLGQLQNMLQQQSTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TAFVYTFGFLLISYLCLIPLLALFKTKPKIETQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]