Click here to switch to the map view.

The map label for this gene is mhpE [C]

Identifier: 167624012

GI number: 167624012

Start: 2513451

End: 2514473

Strand: Reverse

Name: mhpE [C]

Synonym: Shal_2088

Alternate gene names: 167624012

Gene position: 2514473-2513451 (Counterclockwise)

Preceding gene: 167624013

Following gene: 167624011

Centisome position: 48.11

GC content: 45.36

Gene sequence:

>1023_bases
ATGAATTTAACGGGAAAAAAAGTTATTTTACACGACATGTCATTAAGAGATGGCATGCATGCTCGCCAACATCAAATTAC
TTTGAAAGAGATGGTTGATGTAGCAACAGGACTCGATGCTGCGGGGGTACCTTTAATCGAAGTTACCCATGGTGATGGCC
TTGGCGGTGCATCGCTAAATTATGGTTTCCCTGCGCACACTGATGAAGAATACTTAAGCGCCGTGATCCCTAAAATGAAG
CAAGCAAAGGTTTCTGCACTGTTATTACCAGGGATCGGTACCGTCGATCATTTACGGATGGCACATCAACTTGGTGTTAA
TACAATCCGAGTTGCTACCCACTGTACTGAAGCCGATGTATCTGGACAGCACATTGCGTTATCAAGAGATTTAGGCCTTG
ATACTGTAGGATTTCTTATGATGGCTCATATGGTTAGCCCTGAGAAGTTGCTTGAGCAAGCCAAACTTATGGAGTCCTAT
GGCGCAAATTGTATTTACTGCACCGACTCTGCAGGTTATATGCTACCAGGAGACGTTACTTCGCATATTGAGCGATTACG
GGGCGAGCTTAAAAGCGATACGCAAATTGGTTTTCATGGCCACCATAATATGGGGATGAGTATCGCCAACTCCCTTGCAG
CTATTGAAGCTGGCGCCGAACGTATAGATGGTTCCGTTGCTGGTTTGGGTGCCGGAGCTGGAAACACACCATTAGAGGTA
TTCGTGGCCGTACTTGAACGTATGCAGGTCCACCACGGGGTTAACTTATATGACATTATGGACGTAGCAGAAGATCTCGT
TACCCCAATGATGGACCAACCGATAAGAATCGACAGAGATGCGCTAACTTTAGGCTATGCTGGAGTCTATTCATCATTCT
TATTGTTCGCACAAAGAGCCGAAAAAAAATATGGGGTTCCAGCAAGAGATATATTGCTTGAGCTGGGTAGACGTGGCACT
GTTGGCGGCCAAGAAGATATGATTGATGATACCGCAATGAATATGGCAAGAGAGCTTGCCTAA

Upstream 100 bases:

>100_bases
CGCAGCAGCAAGAACAGCAGAGATGCTAGCGTCCAAAATGCTCAATCCTGAACCCCATTACAATGTTACCCAAGAAGCGG
CGCTAGCCTAGGAGTCCATG

Downstream 100 bases:

>100_bases
ACGTGAATAAAATTGCTGACGGTAAACCAACACAGTGGCAGCGACATGGTCTGCTACTGCTGTTGACGTTGTTTTATGGT
GAAACGTTCATCGGAAGACA

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNLTGKKVILHDMSLRDGMHARQHQITLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLNYGFPAHTDEEYLSAVIPKMK
QAKVSALLLPGIGTVDHLRMAHQLGVNTIRVATHCTEADVSGQHIALSRDLGLDTVGFLMMAHMVSPEKLLEQAKLMESY
GANCIYCTDSAGYMLPGDVTSHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIEAGAERIDGSVAGLGAGAGNTPLEV
FVAVLERMQVHHGVNLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRAEKKYGVPARDILLELGRRGT
VGGQEDMIDDTAMNMARELA

Sequences:

>Translated_340_residues
MNLTGKKVILHDMSLRDGMHARQHQITLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLNYGFPAHTDEEYLSAVIPKMK
QAKVSALLLPGIGTVDHLRMAHQLGVNTIRVATHCTEADVSGQHIALSRDLGLDTVGFLMMAHMVSPEKLLEQAKLMESY
GANCIYCTDSAGYMLPGDVTSHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIEAGAERIDGSVAGLGAGAGNTPLEV
FVAVLERMQVHHGVNLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRAEKKYGVPARDILLELGRRGT
VGGQEDMIDDTAMNMARELA
>Mature_340_residues
MNLTGKKVILHDMSLRDGMHARQHQITLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLNYGFPAHTDEEYLSAVIPKMK
QAKVSALLLPGIGTVDHLRMAHQLGVNTIRVATHCTEADVSGQHIALSRDLGLDTVGFLMMAHMVSPEKLLEQAKLMESY
GANCIYCTDSAGYMLPGDVTSHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIEAGAERIDGSVAGLGAGAGNTPLEV
FVAVLERMQVHHGVNLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRAEKKYGVPARDILLELGRRGT
VGGQEDMIDDTAMNMARELA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=333, Percent_Identity=55.8558558558559, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786261, Length=303, Percent_Identity=27.3927392739274, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_SHEHH (B0TTS9)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674306.1
- ProteinModelPortal:   B0TTS9
- SMR:   B0TTS9
- GeneID:   5902819
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2088
- HOGENOM:   HBG300122
- OMA:   MMAHMAS
- ProtClustDB:   PRK08195
- BioCyc:   SHAL458817:SHAL_2088-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36600; Mature: 36600

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 170-170 BINDING 199-199 BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTGKKVILHDMSLRDGMHARQHQITLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLN
CCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLSAVIPKMKQAKVSALLLPGIGTVDHLRMAHQLGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
SGQHIALSRDLGLDTVGFLMMAHMVSPEKLLEQAKLMESYGANCIYCTDSAGYMLPGDVT
CCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHH
SHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIEAGAERIDGSVAGLGAGAGNTPLEV
HHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHH
FVAVLERMQVHHGVNLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHH
EKKYGVPARDILLELGRRGTVGGQEDMIDDTAMNMARELA
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLTGKKVILHDMSLRDGMHARQHQITLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLN
CCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLSAVIPKMKQAKVSALLLPGIGTVDHLRMAHQLGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
SGQHIALSRDLGLDTVGFLMMAHMVSPEKLLEQAKLMESYGANCIYCTDSAGYMLPGDVT
CCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHH
SHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIEAGAERIDGSVAGLGAGAGNTPLEV
HHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHH
FVAVLERMQVHHGVNLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHH
EKKYGVPARDILLELGRRGTVGGQEDMIDDTAMNMARELA
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA