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The map label for this gene is fixJ [H]

Identifier: 167623987

GI number: 167623987

Start: 2483023

End: 2483622

Strand: Reverse

Name: fixJ [H]

Synonym: Shal_2061

Alternate gene names: 167623987

Gene position: 2483622-2483023 (Counterclockwise)

Preceding gene: 167623988

Following gene: 167623982

Centisome position: 47.52

GC content: 44.0

Gene sequence:

>600_bases
ATGACTAATCTGTATCTGGTAGACGATGATCAGGCTGTACTAGACTCGTTAACCTGGATGTTAAATGGACTTGGATATCA
ACCTAAAGGCTTTTTATCGGCCGATTCATTCCTACAACAAGTCGATATAAATAACACGGGGATCGCAATCTTAGATGTGC
AGATGCCCGGTATGGATGGCAGTGCGTTATTAACGCATATGAGCAAGGCACAAAGCCCTATTGCCGTAATAATGTTAAGT
GGTCATGGTAGTATCGCTATGGCAGTACAAGCCATTCAAAAAGGTGCGCTAGACTTCTTAGAAAAGCCTGTAGATGGAGA
TAAACTTGTACGGTTGCTTGACCAAGCGGGTGAACTAACAGAGCAAAATCTACGGAGCAAACAGGAGCGCCAAGCGCTTT
CTGATAAGTTAGCGACACTGACACCAAGAGAACATGAGGTGATGGAGAAAGTCTTAGAAGGAAAGCTCAATAAGGTGATC
GCGGCGGAGCTAAATGTCGCTCAGCGTACATTAGAGCTACACCGTCAAAAAGTCATGCTAAAAATGCAGGTCAGTAATGT
TGCTGAACTCGCTTTCTTAATGGGCAAAAGCACCGAATAA

Upstream 100 bases:

>100_bases
GCCGGTGAGATAAAGGCTAAAAACAATTGCAACAAATATACTAAGCAGCGTGAGCCAGGTTTAAGCATGACTATCACCCT
TCCAATTAAGGAACCATTAA

Downstream 100 bases:

>100_bases
CCCTTTACACTAATTGGGATTAGCCCCTATGCAATTCTACTGTCTGGGCTACGTTTTGATTTTGCCCAAGTTGCACAAAA
CTCCCAGCCTCTATCTTGGC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTNLYLVDDDQAVLDSLTWMLNGLGYQPKGFLSADSFLQQVDINNTGIAILDVQMPGMDGSALLTHMSKAQSPIAVIMLS
GHGSIAMAVQAIQKGALDFLEKPVDGDKLVRLLDQAGELTEQNLRSKQERQALSDKLATLTPREHEVMEKVLEGKLNKVI
AAELNVAQRTLELHRQKVMLKMQVSNVAELAFLMGKSTE

Sequences:

>Translated_199_residues
MTNLYLVDDDQAVLDSLTWMLNGLGYQPKGFLSADSFLQQVDINNTGIAILDVQMPGMDGSALLTHMSKAQSPIAVIMLS
GHGSIAMAVQAIQKGALDFLEKPVDGDKLVRLLDQAGELTEQNLRSKQERQALSDKLATLTPREHEVMEKVLEGKLNKVI
AAELNVAQRTLELHRQKVMLKMQVSNVAELAFLMGKSTE
>Mature_198_residues
TNLYLVDDDQAVLDSLTWMLNGLGYQPKGFLSADSFLQQVDINNTGIAILDVQMPGMDGSALLTHMSKAQSPIAVIMLSG
HGSIAMAVQAIQKGALDFLEKPVDGDKLVRLLDQAGELTEQNLRSKQERQALSDKLATLTPREHEVMEKVLEGKLNKVIA
AELNVAQRTLELHRQKVMLKMQVSNVAELAFLMGKSTE

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=202, Percent_Identity=32.6732673267327, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=33.6283185840708, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788905, Length=128, Percent_Identity=37.5, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=34.2105263157895, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21844; Mature: 21712

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLYLVDDDQAVLDSLTWMLNGLGYQPKGFLSADSFLQQVDINNTGIAILDVQMPGMDG
CCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCH
SALLTHMSKAQSPIAVIMLSGHGSIAMAVQAIQKGALDFLEKPVDGDKLVRLLDQAGELT
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQNLRSKQERQALSDKLATLTPREHEVMEKVLEGKLNKVIAAELNVAQRTLELHRQKVML
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMQVSNVAELAFLMGKSTE
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNLYLVDDDQAVLDSLTWMLNGLGYQPKGFLSADSFLQQVDINNTGIAILDVQMPGMDG
CCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCH
SALLTHMSKAQSPIAVIMLSGHGSIAMAVQAIQKGALDFLEKPVDGDKLVRLLDQAGELT
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQNLRSKQERQALSDKLATLTPREHEVMEKVLEGKLNKVIAAELNVAQRTLELHRQKVML
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMQVSNVAELAFLMGKSTE
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]