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The map label for this gene is prpB [H]

Identifier: 167623890

GI number: 167623890

Start: 2357314

End: 2358192

Strand: Direct

Name: prpB [H]

Synonym: Shal_1961

Alternate gene names: 167623890

Gene position: 2357314-2358192 (Clockwise)

Preceding gene: 167623889

Following gene: 167623891

Centisome position: 45.1

GC content: 50.06

Gene sequence:

>879_bases
ATGAGCGCAGGTAAGAAGTTTCGCGAGGCATTAGCCGCTAACAAGCCCCTACAGATAGTAGGCACCATTAATGCCTACTC
AGCCATGATGGCTAAAAAGATTGGTCACCAAGCTATTTACCTTTCGGGTGGCGGTGTGGCTAACGCTTCCTATGGCTTGC
CCGATCTGGGCATGACATCGCTAAACGATGTCATTGTTGATGTGCAGCGCATCACCTCAGCTTGTGACTTGCCGTTGCTG
GTGGATATCGACACGGGTTGGGGCGGTGCATTTAACATTGCAAAAACCATTCGTGATATGGAAAAAGCCGGCGCCGCAGC
GGTGCACATGGAAGATCAAGTGGCGCAGAAGCGCTGTGGTCATCGCCCGAATAAAGAGATCGTTTCGACCCAAGAGATGG
TCGATCGCATTAAAGCGGCGGTCGATGCCCGTAGCGATCCAGACTTTTTCATTATGGCGCGCACCGACTCTTTCGCACAG
GAAGGCTTAGAGGCCGCAATTGCTCGCGCTAAAGCCTATGTGGCTGCAGGGGCTGACGGTATTTTTGCCGAAGCGGTCAA
AACCGAAGAGCATTACCGCGCCTTTAGCGACGCTCTTGATGTACCTATTCTTGCCAACATTACCGAGTTTGGCCAAACCG
AGTTGTGGAACAAAGAGCAACTAGGCGAGTGGGGCGCATCTATGGTGCTGTATCCATTAAGTGCATTTAGAGCCATGAAT
AAAGCCGCCGAGATGGTATACACCTCAATCTTAGAAAATGGCGACCAAAAAGCGGTTGTTGATTCGATGCAAACCCGCAT
GGATCTGTATGACTACCTGGGTTATCACGATTACGAGCAGAAGTTAGATAAGCTGTTCTCAGAAGGTAAGAATTTATAA

Upstream 100 bases:

>100_bases
CATAACAAATCAATAATTGACAGTAATAACATCATTATAAATTTTGCCGTGTAGCAGCTAACACTCGAGCACAGTGAACG
ATAAATAAAAGGATCAGCAC

Downstream 100 bases:

>100_bases
ATAGCGCGACATAAATGCGTTAGCCCCTGTTGAACAAAGCAATACCTTACAAAGTAAATCATCAAAGTAAACCCTACAAG
TTTACAAGTGGAAGCGTTAA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSAGKKFREALAANKPLQIVGTINAYSAMMAKKIGHQAIYLSGGGVANASYGLPDLGMTSLNDVIVDVQRITSACDLPLL
VDIDTGWGGAFNIAKTIRDMEKAGAAAVHMEDQVAQKRCGHRPNKEIVSTQEMVDRIKAAVDARSDPDFFIMARTDSFAQ
EGLEAAIARAKAYVAAGADGIFAEAVKTEEHYRAFSDALDVPILANITEFGQTELWNKEQLGEWGASMVLYPLSAFRAMN
KAAEMVYTSILENGDQKAVVDSMQTRMDLYDYLGYHDYEQKLDKLFSEGKNL

Sequences:

>Translated_292_residues
MSAGKKFREALAANKPLQIVGTINAYSAMMAKKIGHQAIYLSGGGVANASYGLPDLGMTSLNDVIVDVQRITSACDLPLL
VDIDTGWGGAFNIAKTIRDMEKAGAAAVHMEDQVAQKRCGHRPNKEIVSTQEMVDRIKAAVDARSDPDFFIMARTDSFAQ
EGLEAAIARAKAYVAAGADGIFAEAVKTEEHYRAFSDALDVPILANITEFGQTELWNKEQLGEWGASMVLYPLSAFRAMN
KAAEMVYTSILENGDQKAVVDSMQTRMDLYDYLGYHDYEQKLDKLFSEGKNL
>Mature_291_residues
SAGKKFREALAANKPLQIVGTINAYSAMMAKKIGHQAIYLSGGGVANASYGLPDLGMTSLNDVIVDVQRITSACDLPLLV
DIDTGWGGAFNIAKTIRDMEKAGAAAVHMEDQVAQKRCGHRPNKEIVSTQEMVDRIKAAVDARSDPDFFIMARTDSFAQE
GLEAAIARAKAYVAAGADGIFAEAVKTEEHYRAFSDALDVPILANITEFGQTELWNKEQLGEWGASMVLYPLSAFRAMNK
AAEMVYTSILENGDQKAVVDSMQTRMDLYDYLGYHDYEQKLDKLFSEGKNL

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=290, Percent_Identity=61.0344827586207, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790445, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31909; Mature: 31777

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGKKFREALAANKPLQIVGTINAYSAMMAKKIGHQAIYLSGGGVANASYGLPDLGMTS
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHH
LNDVIVDVQRITSACDLPLLVDIDTGWGGAFNIAKTIRDMEKAGAAAVHMEDQVAQKRCG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHC
HRPNKEIVSTQEMVDRIKAAVDARSDPDFFIMARTDSFAQEGLEAAIARAKAYVAAGADG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCH
IFAEAVKTEEHYRAFSDALDVPILANITEFGQTELWNKEQLGEWGASMVLYPLSAFRAMN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KAAEMVYTSILENGDQKAVVDSMQTRMDLYDYLGYHDYEQKLDKLFSEGKNL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAGKKFREALAANKPLQIVGTINAYSAMMAKKIGHQAIYLSGGGVANASYGLPDLGMTS
CCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHH
LNDVIVDVQRITSACDLPLLVDIDTGWGGAFNIAKTIRDMEKAGAAAVHMEDQVAQKRCG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHC
HRPNKEIVSTQEMVDRIKAAVDARSDPDFFIMARTDSFAQEGLEAAIARAKAYVAAGADG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCH
IFAEAVKTEEHYRAFSDALDVPILANITEFGQTELWNKEQLGEWGASMVLYPLSAFRAMN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KAAEMVYTSILENGDQKAVVDSMQTRMDLYDYLGYHDYEQKLDKLFSEGKNL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]