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The map label for this gene is thiF [H]

Identifier: 167623832

GI number: 167623832

Start: 2286559

End: 2287446

Strand: Direct

Name: thiF [H]

Synonym: Shal_1902

Alternate gene names: 167623832

Gene position: 2286559-2287446 (Clockwise)

Preceding gene: 167623831

Following gene: 167623833

Centisome position: 43.75

GC content: 46.62

Gene sequence:

>888_bases
ATGTTTAAAGGTGCAATAAATAGGTCATCAGACGAGATAAGCCTATCTCTTAGTGATAGCGATTTTTTACATTATTCACG
ACAGGTATTGCTACCAGAAGTCGGTGAGGCAGGGCAGATCTTACTGGCTAATGCCCATGTCGCGGTAATAGGCATTGGCG
GTTTGGGCAATGTAGCGGCCCAGTATCTGGCTGCTGCAGGAGTTGGTCACATAACGCTGGTGGATGGTGATGATGTGGAG
GTATCAAACCTTCCTCGGCAACTGTTGTTTACCGTTAGCGATATTGGCGGCAATAAAGCGCAGGTAGCACAGAAAAAACT
TAGCCATGCCTACACTCAAGTTAAGTTAGCAGCCGTTACTGATTACTTAACGAAAGACAATATCCAGCAAGCTTTAAGCA
TACAAGCTCTTGGCATACAAGAAATGGGCATAGAGGCTGGCCAATATGATCTGCTGCTAGATTGCAGTGACAACTTTGAA
ACCCGGCAATTGGTTAATAGGTTTGCCATTAGCAATCAGCTGCCATTAGTGTCGGCGTCAGCGGCGCATTTTCAAGGACA
ACTGCTCAATATCGATCAACGACACAGCCCCCAATCTGGCTGTTATCACTGTTTATTTCCTGCCGATATGCAAGTCGGTC
AAAGCTGTCAAACCGTGGGCGTGCTGGGGCCAATGGTTGGCACACTTGCATCGATGCAAGCCTTAATGGCGTTGCAACAG
CTATTAGGCCATCGGGACACCGTCGGTAAATTATTTCGCTTTGACGGTAAGCAATTTAGCTGGCGAGAAGCAAGACTTCC
ACGTAGCAAAGACTGCCTTGTATGCCGCGCTGACAGTAATAATAACAATGGCAATGGCAATGACTGTGAACCTGAATATC
GAAGTTAA

Upstream 100 bases:

>100_bases
GTGTGGATGATATTGCCGTGGTGCGGGCGGTGACAGAGTCAGACGACCCTGCTGCTGCATTTAAAGCGTTAGCGCAAGCA
TGGCTTGAGGAGTCATGATT

Downstream 100 bases:

>100_bases
ATTAGCATGGTACGGATGTAAACCTGTAGAGGTAGATTATGCAGCAAGAAAACAATAATGAAGCGGGATCAGTAGTGAAG
GTGGAAGCCCAAAGTGGCAT

Product: UBA/THIF-type NAD/FAD-binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MFKGAINRSSDEISLSLSDSDFLHYSRQVLLPEVGEAGQILLANAHVAVIGIGGLGNVAAQYLAAAGVGHITLVDGDDVE
VSNLPRQLLFTVSDIGGNKAQVAQKKLSHAYTQVKLAAVTDYLTKDNIQQALSIQALGIQEMGIEAGQYDLLLDCSDNFE
TRQLVNRFAISNQLPLVSASAAHFQGQLLNIDQRHSPQSGCYHCLFPADMQVGQSCQTVGVLGPMVGTLASMQALMALQQ
LLGHRDTVGKLFRFDGKQFSWREARLPRSKDCLVCRADSNNNNGNGNDCEPEYRS

Sequences:

>Translated_295_residues
MFKGAINRSSDEISLSLSDSDFLHYSRQVLLPEVGEAGQILLANAHVAVIGIGGLGNVAAQYLAAAGVGHITLVDGDDVE
VSNLPRQLLFTVSDIGGNKAQVAQKKLSHAYTQVKLAAVTDYLTKDNIQQALSIQALGIQEMGIEAGQYDLLLDCSDNFE
TRQLVNRFAISNQLPLVSASAAHFQGQLLNIDQRHSPQSGCYHCLFPADMQVGQSCQTVGVLGPMVGTLASMQALMALQQ
LLGHRDTVGKLFRFDGKQFSWREARLPRSKDCLVCRADSNNNNGNGNDCEPEYRS
>Mature_295_residues
MFKGAINRSSDEISLSLSDSDFLHYSRQVLLPEVGEAGQILLANAHVAVIGIGGLGNVAAQYLAAAGVGHITLVDGDDVE
VSNLPRQLLFTVSDIGGNKAQVAQKKLSHAYTQVKLAAVTDYLTKDNIQQALSIQALGIQEMGIEAGQYDLLLDCSDNFE
TRQLVNRFAISNQLPLVSASAAHFQGQLLNIDQRHSPQSGCYHCLFPADMQVGQSCQTVGVLGPMVGTLASMQALMALQQ
LLGHRDTVGKLFRFDGKQFSWREARLPRSKDCLVCRADSNNNNGNGNDCEPEYRS

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=192, Percent_Identity=43.2291666666667, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4885649, Length=175, Percent_Identity=30.2857142857143, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082356, Length=259, Percent_Identity=40.5405405405405, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787048, Length=261, Percent_Identity=34.0996168582375, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789177, Length=151, Percent_Identity=32.4503311258278, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17540406, Length=271, Percent_Identity=34.3173431734317, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321903, Length=277, Percent_Identity=34.6570397111913, Blast_Score=122, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24582879, Length=270, Percent_Identity=38.1481481481481, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24660640, Length=173, Percent_Identity=30.635838150289, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 31905; Mature: 31905

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGAINRSSDEISLSLSDSDFLHYSRQVLLPEVGEAGQILLANAHVAVIGIGGLGNVAA
CCCCCCCCCCCEEEEEECCCHHHHHHHHEECCCCCCCCEEEEEECEEEEEEECCHHHHHH
QYLAAAGVGHITLVDGDDVEVSNLPRQLLFTVSDIGGNKAQVAQKKLSHAYTQVKLAAVT
HHHHHCCCCEEEEECCCCCCHHHCCHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DYLTKDNIQQALSIQALGIQEMGIEAGQYDLLLDCSDNFETRQLVNRFAISNQLPLVSAS
HHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEECC
AAHFQGQLLNIDQRHSPQSGCYHCLFPADMQVGQSCQTVGVLGPMVGTLASMQALMALQQ
HHHHCCEEECCCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGHRDTVGKLFRFDGKQFSWREARLPRSKDCLVCRADSNNNNGNGNDCEPEYRS
HHCCCHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKGAINRSSDEISLSLSDSDFLHYSRQVLLPEVGEAGQILLANAHVAVIGIGGLGNVAA
CCCCCCCCCCCEEEEEECCCHHHHHHHHEECCCCCCCCEEEEEECEEEEEEECCHHHHHH
QYLAAAGVGHITLVDGDDVEVSNLPRQLLFTVSDIGGNKAQVAQKKLSHAYTQVKLAAVT
HHHHHCCCCEEEEECCCCCCHHHCCHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DYLTKDNIQQALSIQALGIQEMGIEAGQYDLLLDCSDNFETRQLVNRFAISNQLPLVSAS
HHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEECC
AAHFQGQLLNIDQRHSPQSGCYHCLFPADMQVGQSCQTVGVLGPMVGTLASMQALMALQQ
HHHHCCEEECCCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGHRDTVGKLFRFDGKQFSWREARLPRSKDCLVCRADSNNNNGNGNDCEPEYRS
HHCCCHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]