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The map label for this gene is thiE [H]

Identifier: 167623831

GI number: 167623831

Start: 2284943

End: 2286556

Strand: Direct

Name: thiE [H]

Synonym: Shal_1901

Alternate gene names: 167623831

Gene position: 2284943-2286556 (Clockwise)

Preceding gene: 167623830

Following gene: 167623832

Centisome position: 43.71

GC content: 49.07

Gene sequence:

>1614_bases
ATGACAAGTGCTCGTCAAGTTTTGCCTGCTGTTCGTCCGATTGTGTGGACGATTGCGGGCTCTGATAGCGGAGGCGGCGC
GGGTATTCAAGCAGATCTGGCAACCATGTCAGATCTGGACACCCACGCGTGTAGCGTTATCAGCGCAGTGACCGCGCAAA
GCTCGGTTACAGTGTCGTTAGTCGAGGCGGTATCGGCGCAAATGCTGAGCTCTCAGCTCAATACTTTGGTAACAGACCTA
CCGCCAGATGCGATTAAAATCGGTTTACTCGCTGATCAGAGGCAAATCGCTTTATTAGCATTATGGTTACGTGATCAACA
AGATATTTGGCTTAAAGCTGGTGTTAAGGTGCCGGTGATCTTAGATCCCGTGATGGTTGCAACCTGTGGTGATGCTTTAG
CAAATGGGGGAGCGTTAGATTTTACCCCTTTCAAAGGGTTGCTGACTCTTATCACACCCAATGTTAGTGAGCTTGAAGTG
CTTGCGGGCCATTGCTTAGACGATGTCGGCGCTTGTATTACCGCTGCCAAAAAACTCGCCGATGTTCTTGCAACGTCAGT
ACTGGCAAAAGGCGGCGATCGGGGCCCAAGTTGGTGCCAGCATCAGGCCAATGATTTACTGGTTTGTCGCGATGTGAATG
GTATATCGGCTAAGCATCAGTGGCAAAGTTTTTGGCTATCGAGCATAAGAGAAGCCAGTGGTAATAATCATGGCACTGGC
TGCACATTATCATCGGCCATTGCGGCTGTGATGGCTCATGGCTTTGTGCTTAATGATGCCGTCGTTGTGGCTAAAGCCTA
TGTGAGTGCGGGTCTTCGCGGGAGTTATCAACCAGGGAAAGGAGCGGGCTGCCTAGCAAGAACGGCGTGGCCAAGGGAGT
TAAGCTTATTTCCTTATATCAAACAAGTCACACAGCAAGACCCATTACCGGCTGCATTGATTAACAATGCGCTATCGAGG
ACGGGCAAACTCAAAGGCTTTAAAAAGATTGATGAAGCCTTGGGGCTGTATCCAGTTGTCGCAGATGTTGCTTTGCTCGA
AATGCTATTAAAAGCGGGGGCTAAGACACTACAGCTGCGAATAAAAGATACCAAGGAGCAGGCTCTTAAGGAGCAATCAC
TTGAGCAGCAGATCATTCAAGCAATAGCCCTTGGCCGTGATTATCAGGCGCGAGTGTTTATCAATGATCATTGGCAGTTA
GCGATAAAGCATCAGGCTTACGGAGTACATTTGGGTCAGGAAGACTTGATTGAGAGTAATCTTAAGCAACTTGATGACGC
GGATATTGCTCTTGGACTGTCGAGTCATAGTTATTTTGAGATCTTGCTCGCCAAGCAGCACAACCCATCTTATATCGCAT
TTGGTCATATATTTCCGACAACGACCAAGGTGATGCCCTCTAAACCGCAGGGACTCGCAAAACTGGCAAGGTATGTGTCT
TTGATGAAGGGCGAGCTTCCTATCGTCGCTATTGGTGGTATCGATGCATCGAGGCTTGCAAGTGTGAAAGCAACAGGTGT
GGATGATATTGCCGTGGTGCGGGCGGTGACAGAGTCAGACGACCCTGCTGCTGCATTTAAAGCGTTAGCGCAAGCATGGC
TTGAGGAGTCATGA

Upstream 100 bases:

>100_bases
TGGGCATGGCGCAGATGTCAGCCGAGTTTAAGGCTAAGGGCGCAGAGCTCTATCATGAAGCCGCCGATAAACAGCGTGAT
GCGGTATTAGAGGACTAGTC

Downstream 100 bases:

>100_bases
TTATGTTTAAAGGTGCAATAAATAGGTCATCAGACGAGATAAGCCTATCTCTTAGTGATAGCGATTTTTTACATTATTCA
CGACAGGTATTGCTACCAGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTSARQVLPAVRPIVWTIAGSDSGGGAGIQADLATMSDLDTHACSVISAVTAQSSVTVSLVEAVSAQMLSSQLNTLVTDL
PPDAIKIGLLADQRQIALLALWLRDQQDIWLKAGVKVPVILDPVMVATCGDALANGGALDFTPFKGLLTLITPNVSELEV
LAGHCLDDVGACITAAKKLADVLATSVLAKGGDRGPSWCQHQANDLLVCRDVNGISAKHQWQSFWLSSIREASGNNHGTG
CTLSSAIAAVMAHGFVLNDAVVVAKAYVSAGLRGSYQPGKGAGCLARTAWPRELSLFPYIKQVTQQDPLPAALINNALSR
TGKLKGFKKIDEALGLYPVVADVALLEMLLKAGAKTLQLRIKDTKEQALKEQSLEQQIIQAIALGRDYQARVFINDHWQL
AIKHQAYGVHLGQEDLIESNLKQLDDADIALGLSSHSYFEILLAKQHNPSYIAFGHIFPTTTKVMPSKPQGLAKLARYVS
LMKGELPIVAIGGIDASRLASVKATGVDDIAVVRAVTESDDPAAAFKALAQAWLEES

Sequences:

>Translated_537_residues
MTSARQVLPAVRPIVWTIAGSDSGGGAGIQADLATMSDLDTHACSVISAVTAQSSVTVSLVEAVSAQMLSSQLNTLVTDL
PPDAIKIGLLADQRQIALLALWLRDQQDIWLKAGVKVPVILDPVMVATCGDALANGGALDFTPFKGLLTLITPNVSELEV
LAGHCLDDVGACITAAKKLADVLATSVLAKGGDRGPSWCQHQANDLLVCRDVNGISAKHQWQSFWLSSIREASGNNHGTG
CTLSSAIAAVMAHGFVLNDAVVVAKAYVSAGLRGSYQPGKGAGCLARTAWPRELSLFPYIKQVTQQDPLPAALINNALSR
TGKLKGFKKIDEALGLYPVVADVALLEMLLKAGAKTLQLRIKDTKEQALKEQSLEQQIIQAIALGRDYQARVFINDHWQL
AIKHQAYGVHLGQEDLIESNLKQLDDADIALGLSSHSYFEILLAKQHNPSYIAFGHIFPTTTKVMPSKPQGLAKLARYVS
LMKGELPIVAIGGIDASRLASVKATGVDDIAVVRAVTESDDPAAAFKALAQAWLEES
>Mature_536_residues
TSARQVLPAVRPIVWTIAGSDSGGGAGIQADLATMSDLDTHACSVISAVTAQSSVTVSLVEAVSAQMLSSQLNTLVTDLP
PDAIKIGLLADQRQIALLALWLRDQQDIWLKAGVKVPVILDPVMVATCGDALANGGALDFTPFKGLLTLITPNVSELEVL
AGHCLDDVGACITAAKKLADVLATSVLAKGGDRGPSWCQHQANDLLVCRDVNGISAKHQWQSFWLSSIREASGNNHGTGC
TLSSAIAAVMAHGFVLNDAVVVAKAYVSAGLRGSYQPGKGAGCLARTAWPRELSLFPYIKQVTQQDPLPAALINNALSRT
GKLKGFKKIDEALGLYPVVADVALLEMLLKAGAKTLQLRIKDTKEQALKEQSLEQQIIQAIALGRDYQARVFINDHWQLA
IKHQAYGVHLGQEDLIESNLKQLDDADIALGLSSHSYFEILLAKQHNPSYIAFGHIFPTTTKVMPSKPQGLAKLARYVSL
MKGELPIVAIGGIDASRLASVKATGVDDIAVVRAVTESDDPAAAFKALAQAWLEES

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=53.8461538461538, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=34.2307692307692, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=29.6028880866426, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324997, Length=265, Percent_Identity=29.4339622641509, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324517, Length=268, Percent_Identity=29.1044776119403, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 57052; Mature: 56921

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSARQVLPAVRPIVWTIAGSDSGGGAGIQADLATMSDLDTHACSVISAVTAQSSVTVSL
CCCHHHHHHHHCCEEEEEECCCCCCCCCCEEHHHHHHHCHHHHHHHHHHHHHCCCHHHHH
VEAVSAQMLSSQLNTLVTDLPPDAIKIGLLADQRQIALLALWLRDQQDIWLKAGVKVPVI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCEE
LDPVMVATCGDALANGGALDFTPFKGLLTLITPNVSELEVLAGHCLDDVGACITAAKKLA
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLATSVLAKGGDRGPSWCQHQANDLLVCRDVNGISAKHQWQSFWLSSIREASGNNHGTG
HHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
CTLSSAIAAVMAHGFVLNDAVVVAKAYVSAGLRGSYQPGKGAGCLARTAWPRELSLFPYI
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHHHH
KQVTQQDPLPAALINNALSRTGKLKGFKKIDEALGLYPVVADVALLEMLLKAGAKTLQLR
HHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEE
IKDTKEQALKEQSLEQQIIQAIALGRDYQARVFINDHWQLAIKHQAYGVHLGQEDLIESN
EECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEECCHHHHHHHH
LKQLDDADIALGLSSHSYFEILLAKQHNPSYIAFGHIFPTTTKVMPSKPQGLAKLARYVS
HHHCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LMKGELPIVAIGGIDASRLASVKATGVDDIAVVRAVTESDDPAAAFKALAQAWLEES
HHCCCCCEEEECCCCHHHHHHEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSARQVLPAVRPIVWTIAGSDSGGGAGIQADLATMSDLDTHACSVISAVTAQSSVTVSL
CCHHHHHHHHCCEEEEEECCCCCCCCCCEEHHHHHHHCHHHHHHHHHHHHHCCCHHHHH
VEAVSAQMLSSQLNTLVTDLPPDAIKIGLLADQRQIALLALWLRDQQDIWLKAGVKVPVI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCEE
LDPVMVATCGDALANGGALDFTPFKGLLTLITPNVSELEVLAGHCLDDVGACITAAKKLA
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLATSVLAKGGDRGPSWCQHQANDLLVCRDVNGISAKHQWQSFWLSSIREASGNNHGTG
HHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
CTLSSAIAAVMAHGFVLNDAVVVAKAYVSAGLRGSYQPGKGAGCLARTAWPRELSLFPYI
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHHHH
KQVTQQDPLPAALINNALSRTGKLKGFKKIDEALGLYPVVADVALLEMLLKAGAKTLQLR
HHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEE
IKDTKEQALKEQSLEQQIIQAIALGRDYQARVFINDHWQLAIKHQAYGVHLGQEDLIESN
EECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEECCHHHHHHHH
LKQLDDADIALGLSSHSYFEILLAKQHNPSYIAFGHIFPTTTKVMPSKPQGLAKLARYVS
HHHCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LMKGELPIVAIGGIDASRLASVKATGVDDIAVVRAVTESDDPAAAFKALAQAWLEES
HHCCCCCEEEECCCCHHHHHHEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA