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The map label for this gene is def [H]

Identifier: 167623826

GI number: 167623826

Start: 2279175

End: 2279666

Strand: Direct

Name: def [H]

Synonym: Shal_1896

Alternate gene names: 167623826

Gene position: 2279175-2279666 (Clockwise)

Preceding gene: 167623824

Following gene: 167623827

Centisome position: 43.6

GC content: 44.31

Gene sequence:

>492_bases
ATGGCAGTGTTAGACATTTTAACGATCCCCGATGAGCGCCTAAAACGCAAAGCGGTTCCTGTAACAGATATTGAGTCTGT
TCAAGGTTTTATCGATGATCTGATTGAGACTATGTATGACACCGACGACGGTATCGGCCTAGCAGCAACTCAGGTTGGTA
GCTTACATGCTATCTTAGTTATCGACCTGTCTGATGAGCGCGATCAGCCAATGGTATTGATTAACCCTGAGTTCCTCGAG
TCTCGTGGTGAATACCAAGGCGAAGAGGGCTGTCTATCTATCCCAGGTTATCGCGCCAAAGTGAATCGCCACCAAGGTGT
GAAGGTGACGGCACTGGACCGTCATGGTAAAGCATTCGAAATCGATACCGATGAATTCTTAGCGATTGTATTACAGCATG
AAATGGATCACCTTAATGGTGTGGTATTTACAGACCATCTCTCTATGCTTAAGCAACAGGTTGCTTTAAAGAAAGTTAAA
AAATACAAGTAA

Upstream 100 bases:

>100_bases
TAACTTAACTAGAGTTAAATTACCGCAGTGGTTGCCTTTGCGGTTGTTTCGGTCATAATAGCGCGAAATTTTTTTAGTTT
ACTTTTAGTGTGATGATAAT

Downstream 100 bases:

>100_bases
GCAAACTGTGTGCTGCTAAGCTGTTAATGGGCTAGCTAAGAAAATCACAGTTGTAAAATTAAGCGCACCTCTTATGAGGG
GGCGCTTTTTTTATGGCTCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 3; Polypeptide deformylase 3 [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MAVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILVIDLSDERDQPMVLINPEFLE
SRGEYQGEEGCLSIPGYRAKVNRHQGVKVTALDRHGKAFEIDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVK
KYK

Sequences:

>Translated_163_residues
MAVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILVIDLSDERDQPMVLINPEFLE
SRGEYQGEEGCLSIPGYRAKVNRHQGVKVTALDRHGKAFEIDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVK
KYK
>Mature_162_residues
AVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILVIDLSDERDQPMVLINPEFLES
RGEYQGEEGCLSIPGYRAKVNRHQGVKVTALDRHGKAFEIDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVKK
YK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=56.1728395061728, Blast_Score=172, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24645728, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18287; Mature: 18156

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILV
CCEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
IDLSDERDQPMVLINPEFLESRGEYQGEEGCLSIPGYRAKVNRHQGVKVTALDRHGKAFE
EEECCCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCEEECCCCCCEEEEEECCCCCEEE
IDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVKKYK
ECHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILV
CEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
IDLSDERDQPMVLINPEFLESRGEYQGEEGCLSIPGYRAKVNRHQGVKVTALDRHGKAFE
EEECCCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCEEECCCCCCEEEEEECCCCCEEE
IDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVKKYK
ECHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]