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The map label for this gene is purN [H]

Identifier: 167623610

GI number: 167623610

Start: 2014017

End: 2014661

Strand: Reverse

Name: purN [H]

Synonym: Shal_1679

Alternate gene names: 167623610

Gene position: 2014661-2014017 (Counterclockwise)

Preceding gene: 167623611

Following gene: 167623609

Centisome position: 38.54

GC content: 45.89

Gene sequence:

>645_bases
ATGCCCCAGAGTTGTCGCGTTTTAGTATTAATTTCAGGTAATGGCAGTAATTTACAAGCCATTATCGATGGCTGTGATGA
TAACCTGCAAGCCGATGTGGTTGGAGTGATCAGTAATAAGCCCGATGCCTATGGATTGGTTCGCGCCCACCAAAATGAGA
TTGATACCAGCTGCGTGATAGCTCACACAGGCGAAACAAGACAAGAGTACGACGCAAGACTACTGAATGCGATTGAAAAG
TACCAACCCGATCTTATCGTGTTGGCTGGCTTTATGCGTATTTTAAGTGATGAATTCGTTCAGCGTTTTGAAGGCAAGAT
GGTTAACATCCACCCTTCTCTCTTGCCTAAATACACAGGATTACACACTCATCAGCGTGCTATTGACGCAAAAGATACAG
AGCACGGCGCAAGCGTGCACTTTGTGACGCCTGAGCTCGATGCTGGCCCTGTCATCTTACAAGCTAAAGTGCCAGTTTAT
GAAGACGACACCGCAGATACATTAGCTGAACGTGTTCATGAGCAAGAGCATGCCATTTACCCACTAGTAGTTAAATGGTT
CAGTCAAAACCGACTCGAAATGGTTGATGGCGTTGCCCAGCTTGATGGCCAAGTATTAGCAATCAATGGTTACGCTGCGG
ACTAA

Upstream 100 bases:

>100_bases
ATTCAGCCTTAGCGTTGCTAAAAACAGAAGGCGAGAACGCATGGTTAATCGGAAACATTGCCAAGCGTAACGGCGAAGAA
GAGCAAGTGGAGATCCTGTA

Downstream 100 bases:

>100_bases
TCTGTTTCAGAAAATGTAGCTTCGTAAGAAAGGCACCTATGGGTGCCTTTCTTGTTCCAGATTTTTAAATCTGCGCGAGA
CTAATACCAATCAGTATAAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPQSCRVLVLISGNGSNLQAIIDGCDDNLQADVVGVISNKPDAYGLVRAHQNEIDTSCVIAHTGETRQEYDARLLNAIEK
YQPDLIVLAGFMRILSDEFVQRFEGKMVNIHPSLLPKYTGLHTHQRAIDAKDTEHGASVHFVTPELDAGPVILQAKVPVY
EDDTADTLAERVHEQEHAIYPLVVKWFSQNRLEMVDGVAQLDGQVLAINGYAAD

Sequences:

>Translated_214_residues
MPQSCRVLVLISGNGSNLQAIIDGCDDNLQADVVGVISNKPDAYGLVRAHQNEIDTSCVIAHTGETRQEYDARLLNAIEK
YQPDLIVLAGFMRILSDEFVQRFEGKMVNIHPSLLPKYTGLHTHQRAIDAKDTEHGASVHFVTPELDAGPVILQAKVPVY
EDDTADTLAERVHEQEHAIYPLVVKWFSQNRLEMVDGVAQLDGQVLAINGYAAD
>Mature_213_residues
PQSCRVLVLISGNGSNLQAIIDGCDDNLQADVVGVISNKPDAYGLVRAHQNEIDTSCVIAHTGETRQEYDARLLNAIEKY
QPDLIVLAGFMRILSDEFVQRFEGKMVNIHPSLLPKYTGLHTHQRAIDAKDTEHGASVHFVTPELDAGPVILQAKVPVYE
DDTADTLAERVHEQEHAIYPLVVKWFSQNRLEMVDGVAQLDGQVLAINGYAAD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=43.5233160621762, Blast_Score=159, Evalue=1e-39,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=43.5233160621762, Blast_Score=159, Evalue=1e-39,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=43.5233160621762, Blast_Score=159, Evalue=1e-39,
Organism=Escherichia coli, GI1788846, Length=209, Percent_Identity=58.8516746411483, Blast_Score=251, Evalue=2e-68,
Organism=Escherichia coli, GI1787483, Length=164, Percent_Identity=32.9268292682927, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=38.4180790960452, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=31.6326530612245, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=51.6666666666667, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23614; Mature: 23483

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSCRVLVLISGNGSNLQAIIDGCDDNLQADVVGVISNKPDAYGLVRAHQNEIDTSCVI
CCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCEEEE
AHTGETRQEYDARLLNAIEKYQPDLIVLAGFMRILSDEFVQRFEGKMVNIHPSLLPKYTG
EECCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCC
LHTHQRAIDAKDTEHGASVHFVTPELDAGPVILQAKVPVYEDDTADTLAERVHEQEHAIY
CCHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHH
PLVVKWFSQNRLEMVDGVAQLDGQVLAINGYAAD
HHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCC
>Mature Secondary Structure 
PQSCRVLVLISGNGSNLQAIIDGCDDNLQADVVGVISNKPDAYGLVRAHQNEIDTSCVI
CCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCEEEE
AHTGETRQEYDARLLNAIEKYQPDLIVLAGFMRILSDEFVQRFEGKMVNIHPSLLPKYTG
EECCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCC
LHTHQRAIDAKDTEHGASVHFVTPELDAGPVILQAKVPVYEDDTADTLAERVHEQEHAIY
CCHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHH
PLVVKWFSQNRLEMVDGVAQLDGQVLAINGYAAD
HHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]