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The map label for this gene is asnB [H]

Identifier: 167623604

GI number: 167623604

Start: 2002997

End: 2004661

Strand: Reverse

Name: asnB [H]

Synonym: Shal_1673

Alternate gene names: 167623604

Gene position: 2004661-2002997 (Counterclockwise)

Preceding gene: 167623605

Following gene: 167623599

Centisome position: 38.35

GC content: 47.63

Gene sequence:

>1665_bases
ATGTGTTCAATTTTTGCAATTTTAGATATTCAATCTGACGCGACGCCACTGCGTCAAGTCGCTCTAGAGATGTCCAAACT
ACTGCGCCACCGTGGTCCAGATTGGTCGGGAATATACAGCAGTGACAAGGCCGTTTTAGCCCATGAGCGTTTAGCTATCG
TCGATATCGAACACGGCGCACAGCCATTACTTAGCCAAGATGAGAGCATTGTTTTAGCGGTTAATGGTGAGATCTACAAC
CACAAGGAGCTAAAAGCCGAGCTGGGTGACAAATACAGCTACCAAACAAACTCCGATTGCGAAGTGATCTTGGCGCTATA
TCAAGAATACGGCACTGAATTCTTAGATAAGCTCAACGGTATTTTTGCCTTCGTACTTTACGACAAGGCGCGTGATACCT
ATCTCGTCGGTCGCGACCATATGGGAATTATCCCGCTCTACACCGGCCGTGATGCAGAAGGTAACTTCTATGTTGCTTCT
GAGATGAAAGCACTCATGCCAGTATGTAAAACCGTAGAGACTTTTACCCCTGGCCACTACCTCTCAAGTGAGAAAGGTGA
ACTGACTCATTATTATCAACGTGAATGGCGTGAATTTGCAGCAGTACAAGATAACCCTGCCAGCGCGGAAGATGTTCGTA
TTGCATTAGAAGCCGCGGTTAAGCGTCAATTGATGTCTGATGTGCCTTACGGTGTTTTATTGTCGGGTGGACTTGATTCA
TCGGTAATTTCAGCTATTACACAAACCTTTGCTAAGCGCCGTATCGAAGATGACGGCGAGAGCAACGCTTGGTGGCCACA
GCTTCACTCATTTGCCGTTGGCCTTGAGGGTGCGCCTGACTTGATTGCCGCAAAAAAGGTGGCTGATGCCATTGGCACTA
TCCATCACGAAATCACCTTTACCTTCCAAGATGGTATCGATGCGATTAAAGACGTGATCTACCACCTTGAAACGTATGAC
GTCACTACCATTCGAGCGGCGACACCTATGTACTTGATGGCACGTAAGATTAAAGCTATGGGCATTAAGATGGTGTTATC
TGGTGAAGGTGCCGATGAATTGTTTGGCGGTTATCTTTACTTCCATAAGGCGCCAAACGCTCAAGCTTTTCACGAGGAGC
TTGTGCGTAAGCTAGACAAACTGTATCTGTTCGATTGTCTACGTGCGAACAAGGCGATGGCGGCATGGGGACTTGAAGCG
CGCGTACCTTTCCTAGACAAAGAGTTTATGGATGTGGCTATGCGCACTAACCCAGAAGCAAAAATGTCCAAGAACGGCAA
GATTGAAAAGCACATTTTACGTGAAGCTTTTGAGCACAAACTGCCTAAAGAGGTGGCTTGGCGCCAAAAGGAGCAGTTCA
GTGATGGTGTAGGTTACTCTTGGATCGATGGTCTTAAAGATCACGCCGCGCAAAAGGTAGACTGTCTGCAATTGGCCAAC
GCTAAGTTTAGATTCCCATACAACACGCCAGAAACGAAAGAAGCCTACTTCTACCGTACTTTTTTTGAGGAGTTGTTCCC
ACTACCAACTGCCGCAGAAACCGTACCAGGTGGTAAGTCGGTAGCTTGCTCAACCCCAGAAGCACTAGCCTGGGATGAAA
GCTTACAAGGGCTCATCGATCCATCTGGCCGCGCGGTGCAATCTGTACACGACTGCGCCTACTAA

Upstream 100 bases:

>100_bases
CTTTTGTAGAGAAATCATCAAAAATAGATTTGAATTGTTGAGCTTTCAATGGCAGATTAATACTCAATTGAGTTTAAGAC
AGAATTTTTTGGAGTGACCT

Downstream 100 bases:

>100_bases
TCAGTATAACGCTCTGTTTAATATACTATAGCCACTCATTTGAGTGGCTATTTTCGTTTTCGAAATGAGCATGTTCGAGG
ATTAAATACGTTAACCAATA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFAILDIQSDATPLRQVALEMSKLLRHRGPDWSGIYSSDKAVLAHERLAIVDIEHGAQPLLSQDESIVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKARDTYLVGRDHMGIIPLYTGRDAEGNFYVAS
EMKALMPVCKTVETFTPGHYLSSEKGELTHYYQREWREFAAVQDNPASAEDVRIALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITFTFQDGIDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLYLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRTNPEAKMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAQKVDCLQLAN
AKFRFPYNTPETKEAYFYRTFFEELFPLPTAAETVPGGKSVACSTPEALAWDESLQGLIDPSGRAVQSVHDCAY

Sequences:

>Translated_554_residues
MCSIFAILDIQSDATPLRQVALEMSKLLRHRGPDWSGIYSSDKAVLAHERLAIVDIEHGAQPLLSQDESIVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKARDTYLVGRDHMGIIPLYTGRDAEGNFYVAS
EMKALMPVCKTVETFTPGHYLSSEKGELTHYYQREWREFAAVQDNPASAEDVRIALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITFTFQDGIDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLYLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRTNPEAKMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAQKVDCLQLAN
AKFRFPYNTPETKEAYFYRTFFEELFPLPTAAETVPGGKSVACSTPEALAWDESLQGLIDPSGRAVQSVHDCAY
>Mature_554_residues
MCSIFAILDIQSDATPLRQVALEMSKLLRHRGPDWSGIYSSDKAVLAHERLAIVDIEHGAQPLLSQDESIVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKARDTYLVGRDHMGIIPLYTGRDAEGNFYVAS
EMKALMPVCKTVETFTPGHYLSSEKGELTHYYQREWREFAAVQDNPASAEDVRIALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITFTFQDGIDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLYLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRTNPEAKMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAQKVDCLQLAN
AKFRFPYNTPETKEAYFYRTFFEELFPLPTAAETVPGGKSVACSTPEALAWDESLQGLIDPSGRAVQSVHDCAY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=40.8839779005525, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=40.8839779005525, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=40.8839779005525, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI296010848, Length=514, Percent_Identity=41.6342412451362, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI296010852, Length=449, Percent_Identity=42.7616926503341, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI296010850, Length=449, Percent_Identity=42.7616926503341, Blast_Score=316, Evalue=3e-86,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=69.1335740072202, Blast_Score=822, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=561, Percent_Identity=42.2459893048128, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=42.2182468694097, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17560178, Length=520, Percent_Identity=36.9230769230769, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI25147560, Length=311, Percent_Identity=38.2636655948553, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=50.4587155963303, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=50.5494505494505, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45553209, Length=575, Percent_Identity=46.4347826086956, Blast_Score=487, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62180; Mature: 62180

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFAILDIQSDATPLRQVALEMSKLLRHRGPDWSGIYSSDKAVLAHERLAIVDIEHGA
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLLSQDESIVLAVNGEIYNHKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CHHHCCCCEEEEEECCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IFAFVLYDKARDTYLVGRDHMGIIPLYTGRDAEGNFYVASEMKALMPVCKTVETFTPGHY
EEEEEEEECCCCEEEECCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSEKGELTHYYQREWREFAAVQDNPASAEDVRIALEAAVKRQLMSDVPYGVLLSGGLDS
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITF
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEE
TFQDGIDAIKDVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLYLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRTNPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAQKVDCLQLAN
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
AKFRFPYNTPETKEAYFYRTFFEELFPLPTAAETVPGGKSVACSTPEALAWDESLQGLID
CEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCEECCCCCHHHHHHHHHHCCC
PSGRAVQSVHDCAY
CCCHHHHHHHHCCC
>Mature Secondary Structure
MCSIFAILDIQSDATPLRQVALEMSKLLRHRGPDWSGIYSSDKAVLAHERLAIVDIEHGA
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLLSQDESIVLAVNGEIYNHKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CHHHCCCCEEEEEECCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IFAFVLYDKARDTYLVGRDHMGIIPLYTGRDAEGNFYVASEMKALMPVCKTVETFTPGHY
EEEEEEEECCCCEEEECCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSEKGELTHYYQREWREFAAVQDNPASAEDVRIALEAAVKRQLMSDVPYGVLLSGGLDS
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITF
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEE
TFQDGIDAIKDVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLYLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRTNPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAQKVDCLQLAN
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
AKFRFPYNTPETKEAYFYRTFFEELFPLPTAAETVPGGKSVACSTPEALAWDESLQGLID
CEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCEECCCCCHHHHHHHHHHCCC
PSGRAVQSVHDCAY
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]