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The map label for this gene is flbD [H]

Identifier: 167623377

GI number: 167623377

Start: 1743584

End: 1744936

Strand: Direct

Name: flbD [H]

Synonym: Shal_1446

Alternate gene names: 167623377

Gene position: 1743584-1744936 (Clockwise)

Preceding gene: 167623376

Following gene: 167623378

Centisome position: 33.36

GC content: 47.45

Gene sequence:

>1353_bases
ATGTCTGAAGGTAAGTTATTGCTTGTAGAAGATGACGCCTCACTGCGCGAGGCGCTGCTCGATACCTTGATGCTAGCGCA
TTATGATTGCGTCGATGTAGGCTCTGCTGAAGAGGCCATTTTAGCGTTAAAAGCAGATCGCTTCGACTTGGTCATTAGTG
ACGTGCAGATGGAGGGCATTGGCGGTATTGGTTTACTCAATTACTTGCAGCAACATCAGCCTAAGCTCCCTGTTTTATTG
ATGACGGCCTATGCCACTATCGACAATGCGGTCAATGCAATGAAACTCGGCGCAGTCGATTATTTGGCTAAGCCTTTCTC
TCCAGAAGTGTTATTAAACCAAGTTTCTCGTTATTTGCCGGCAAAAGCGGCAGAAGGAACCCCCATCGTTGCTGATGAAA
AGAGCTTGGCTCTATTGGCACTCGCACAGCGCGTTGCCGCCTCTGATGCATCAGTAATGATTATGGGCCCAAGTGGCAGT
GGTAAAGAGGTATTAGCGCGTTTTATTCATCAGAATAGCCAGCGAGCAGAGCAACCCTTTATCGCCATTAACTGCGCTGC
TATTCCTGAAAATATGCTTGAAGCCACCCTATTTGGCTATGAAAAGGGGGCATTTACCGGGGCCTATCAAGCGTGCCCAG
GTAAGTTTGAACAAGCACAAGGTGGGACTTTGTTGCTCGATGAGATTTCTGAGATGGAAGTCGGGCTACAGGCAAAACTA
CTACGTGTACTTCAAGAGCGTGAAGTCGAGCGTCTAGGTGGTCGTAAAACCATTAAGCTCGATGTGCGAGTGCTAGCGAC
GTCTAACCGCGACTTAAAGGCGATGGCAGCATCGGGTGAGTTTAGAGAAGATCTTTATTACCGTATTAACGTATTCCCAC
TGACTTGGCCTTCATTGAATCAGCGCCCAGCAGATATCTTGCCCTTGGCAAGGCATCTGCTGCAGCGGCATGCATTGATT
GCTAATCGCAGTGAGTTGCCACTATTGAGCGAATGCGCATCACGTCGGCTATTGACTCATCGTTGGCCTGGTAATGTACG
AGAGCTCGATAATGTGGTTCAAAGAGCGTTGATCATGTGCGGATCACATGAGATCACAGCAGCAGACATTATTATCGATT
CACTTGAGCTCGATTTTAATCTCGAGAGCACACCATTAGCCGACAATAAGCCGACAGAGCTTGATGGTTTAGGTGACGAG
CTTAAAGCGCAAGAGCATGTGATTATTTTAGAAACCTTAACTCAATGTAACGGTAGTCGAAAACTCGTTGCTGAGAAGCT
AGGAATTAGTGCCAGAACCCTGCGTTATAAGATGGCCAAAATGCGTGATTCAGGCATTCAAATTCCGGCGTAA

Upstream 100 bases:

>100_bases
ATGCAGTTCAGTTGTCATCCAGGTAATGGCTGCACTGCGTCATTGAAGTTCCCATTGATCACGGCCATTAATAATAAAGA
AGTGCTTGGAGATAATCAGC

Downstream 100 bases:

>100_bases
TGCAAAGAAGGGCGCTTCGTTTCTAGATCCTAGGACGGACTGCGTCCTTCTATACAATCAAAGACTAAGACGAACGCTTT
TCTCACGGAAGACGGAACGT

Product: Fis family two component sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSEGKLLLVEDDASLREALLDTLMLAHYDCVDVGSAEEAILALKADRFDLVISDVQMEGIGGIGLLNYLQQHQPKLPVLL
MTAYATIDNAVNAMKLGAVDYLAKPFSPEVLLNQVSRYLPAKAAEGTPIVADEKSLALLALAQRVAASDASVMIMGPSGS
GKEVLARFIHQNSQRAEQPFIAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
LRVLQEREVERLGGRKTIKLDVRVLATSNRDLKAMAASGEFREDLYYRINVFPLTWPSLNQRPADILPLARHLLQRHALI
ANRSELPLLSECASRRLLTHRWPGNVRELDNVVQRALIMCGSHEITAADIIIDSLELDFNLESTPLADNKPTELDGLGDE
LKAQEHVIILETLTQCNGSRKLVAEKLGISARTLRYKMAKMRDSGIQIPA

Sequences:

>Translated_450_residues
MSEGKLLLVEDDASLREALLDTLMLAHYDCVDVGSAEEAILALKADRFDLVISDVQMEGIGGIGLLNYLQQHQPKLPVLL
MTAYATIDNAVNAMKLGAVDYLAKPFSPEVLLNQVSRYLPAKAAEGTPIVADEKSLALLALAQRVAASDASVMIMGPSGS
GKEVLARFIHQNSQRAEQPFIAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
LRVLQEREVERLGGRKTIKLDVRVLATSNRDLKAMAASGEFREDLYYRINVFPLTWPSLNQRPADILPLARHLLQRHALI
ANRSELPLLSECASRRLLTHRWPGNVRELDNVVQRALIMCGSHEITAADIIIDSLELDFNLESTPLADNKPTELDGLGDE
LKAQEHVIILETLTQCNGSRKLVAEKLGISARTLRYKMAKMRDSGIQIPA
>Mature_449_residues
SEGKLLLVEDDASLREALLDTLMLAHYDCVDVGSAEEAILALKADRFDLVISDVQMEGIGGIGLLNYLQQHQPKLPVLLM
TAYATIDNAVNAMKLGAVDYLAKPFSPEVLLNQVSRYLPAKAAEGTPIVADEKSLALLALAQRVAASDASVMIMGPSGSG
KEVLARFIHQNSQRAEQPFIAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKLL
RVLQEREVERLGGRKTIKLDVRVLATSNRDLKAMAASGEFREDLYYRINVFPLTWPSLNQRPADILPLARHLLQRHALIA
NRSELPLLSECASRRLLTHRWPGNVRELDNVVQRALIMCGSHEITAADIIIDSLELDFNLESTPLADNKPTELDGLGDEL
KAQEHVIILETLTQCNGSRKLVAEKLGISARTLRYKMAKMRDSGIQIPA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=451, Percent_Identity=40.5764966740577, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=39.0134529147982, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788905, Length=374, Percent_Identity=43.048128342246, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1790299, Length=388, Percent_Identity=39.1752577319588, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI87082117, Length=332, Percent_Identity=43.0722891566265, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789233, Length=304, Percent_Identity=42.1052631578947, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=41.1214953271028, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082152, Length=216, Percent_Identity=47.6851851851852, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=309, Percent_Identity=37.5404530744337, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1786524, Length=312, Percent_Identity=39.1025641025641, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787583, Length=305, Percent_Identity=35.4098360655738, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=37.4501992031873, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081858, Length=244, Percent_Identity=31.1475409836066, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=35.5140186915888, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786784, Length=122, Percent_Identity=36.8852459016393, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49389; Mature: 49258

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGKLLLVEDDASLREALLDTLMLAHYDCVDVGSAEEAILALKADRFDLVISDVQMEGI
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCEEEEEEEEEECCC
GGIGLLNYLQQHQPKLPVLLMTAYATIDNAVNAMKLGAVDYLAKPFSPEVLLNQVSRYLP
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHCC
AKAAEGTPIVADEKSLALLALAQRVAASDASVMIMGPSGSGKEVLARFIHQNSQRAEQPF
CCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCHHCCCE
IAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
EEEEEHHCCHHHHHHHHHCCCCCCCCCCHHCCCCCCHHCCCCEEEEHHHHHHHHHHHHHH
LRVLQEREVERLGGRKTIKLDVRVLATSNRDLKAMAASGEFREDLYYRINVFPLTWPSLN
HHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCCCCC
QRPADILPLARHLLQRHALIANRSELPLLSECASRRLLTHRWPGNVRELDNVVQRALIMC
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHC
GSHEITAADIIIDSLELDFNLESTPLADNKPTELDGLGDELKAQEHVIILETLTQCNGSR
CCCCCEEHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEHHHCCCCH
KLVAEKLGISARTLRYKMAKMRDSGIQIPA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEGKLLLVEDDASLREALLDTLMLAHYDCVDVGSAEEAILALKADRFDLVISDVQMEGI
CCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCEEEEEEEEEECCC
GGIGLLNYLQQHQPKLPVLLMTAYATIDNAVNAMKLGAVDYLAKPFSPEVLLNQVSRYLP
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHCC
AKAAEGTPIVADEKSLALLALAQRVAASDASVMIMGPSGSGKEVLARFIHQNSQRAEQPF
CCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCHHCCCE
IAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
EEEEEHHCCHHHHHHHHHCCCCCCCCCCHHCCCCCCHHCCCCEEEEHHHHHHHHHHHHHH
LRVLQEREVERLGGRKTIKLDVRVLATSNRDLKAMAASGEFREDLYYRINVFPLTWPSLN
HHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCCCCC
QRPADILPLARHLLQRHALIANRSELPLLSECASRRLLTHRWPGNVRELDNVVQRALIMC
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHC
GSHEITAADIIIDSLELDFNLESTPLADNKPTELDGLGDELKAQEHVIILETLTQCNGSR
CCCCCEEHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEHHHCCCCH
KLVAEKLGISARTLRYKMAKMRDSGIQIPA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]