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The map label for this gene is thiH [H]

Identifier: 167623342

GI number: 167623342

Start: 1704310

End: 1705752

Strand: Direct

Name: thiH [H]

Synonym: Shal_1411

Alternate gene names: 167623342

Gene position: 1704310-1705752 (Clockwise)

Preceding gene: 167623341

Following gene: 167623343

Centisome position: 32.61

GC content: 45.32

Gene sequence:

>1443_bases
ATGACTACAAATGAACACCCGCATTCAATCTCCATTTCTCACTACGATGCTAATAAGAGTTTTATTGATGAACAAGCTAT
TTGGGCTGCGATAAATAACGCCGCGAGTCCCTCTGCAGAGCAAGTGCGTCAGGTTCTAGCTAAAGCTCGTCAATGCCAAG
GCCTGAGCATTGAAGAGACAGCTTTGTTATTACAAAACCAGCATGAACAATTAGATGAGGAATTGTTTGCCGTCGCTCGA
GAGGTAAAAAATACCATTTACGGTAACCGCATCGTTCTGTTTGCTCCACTATATGTATCCAATCATTGTGCCAATAGCTG
CAGCTATTGTGGCTTTAATGCAGATAACCATGCACTTAAGCGCAAGACGTTAAATCAAGAAGAAATTAGAAAAGAAGTGA
CCATTTTAGAAGGGATGGGACACAAACGGATTTTAGCCGTATATGGCGAACACCCACGTAACAATGTCAATGCCATTGTT
GATAGCATTCAAACCATGTATAGCGTTAAAGATGGCAAAGGGGGAGAGATCCGCCGCATTAACGTGAACTGTGCGCCGAT
GAGCACCGCTGATTTCAAAGTGCTTAAAACCGCGGCGATAGGAACTTACCAGTGTTTTCAGGAAACATATCACCAACCCA
CCTATGAAAGTGTGCATTTAAAAGGTAAGAAAAAAGACTATCTATACCGTTTATATGCGATGCACCGAGCAATGGAAGCC
GGTATTGATGATGTTGGGATCGGCGCCCTATTTGGGTTATATGACCACCGATTTGAGTTATTAGCCATGTTGACTCATGT
GGCGCAGCTAGAAACCGTTTGCGGAATTGGACCACATACGATTTCGTTCCCACGAATTGAACCTGCACATGGCTCCGCGC
TAAGTGAAAAGCCTCCCTATGAAGTGGACGACGCCTGCTTTAAACGCATCGTGGCCATTACTCGCCTAGCAGTCCCTTAC
ACAGGGCTAATTATGAGTACCCGTGAAAGCGCTGGCTTACGTAAAGAGTTATTAGAACTCGGTGTATCGCAGATCAGTGC
AGGCAGCCGCACATCTCCAGGTGGTTATCAAGACAGCGAGCAAGATCAACATGATGCTGAGCAATTTAGTCTTGGAGATC
ATCGTGCGATGGATGACATTATTTATGAGTTAGTGACAGAGTCGAATGCCATCCCATCATTCTGTACGGGCTGCTACCGT
AAGGGCCGTACTGGCGATCACTTTATAGGCTTAGCGAAACAACAATTTATCGGGAAGTTCTGCCAACCTAACGCATTAAT
CACCTTTAAAGAGTATTTAAATGATTACGCAGGGCCCAAAACCCGTGCAGCAGGTACCGCTCTGATTGAACGAGAACTCG
CAGCGATGAGTCCAGCTAGAGAACGTAACGTACGTACCTGTTTGGCGAAAACCGAAGCTGGCGAACGAGATATCTACCTT
TAA

Upstream 100 bases:

>100_bases
AACAAATAAGTTGATCTTCCCCACCTTCTAACGACAAAGCAGTAGTTACCTTTAGGTAGCTGCTGCTTTTGATTGCTATA
CAAATAGAGTTTTACCGGTT

Downstream 100 bases:

>100_bases
TCTGGCATCAAAATTATTTCACTATAGATAAGGTACCCGATGTGCATTAATGCCATACGTCCATCAGCTGGTCTTGATAA
GAAATCATTATTAACAGCAA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTTNEHPHSISISHYDANKSFIDEQAIWAAINNAASPSAEQVRQVLAKARQCQGLSIEETALLLQNQHEQLDEELFAVAR
EVKNTIYGNRIVLFAPLYVSNHCANSCSYCGFNADNHALKRKTLNQEEIRKEVTILEGMGHKRILAVYGEHPRNNVNAIV
DSIQTMYSVKDGKGGEIRRINVNCAPMSTADFKVLKTAAIGTYQCFQETYHQPTYESVHLKGKKKDYLYRLYAMHRAMEA
GIDDVGIGALFGLYDHRFELLAMLTHVAQLETVCGIGPHTISFPRIEPAHGSALSEKPPYEVDDACFKRIVAITRLAVPY
TGLIMSTRESAGLRKELLELGVSQISAGSRTSPGGYQDSEQDQHDAEQFSLGDHRAMDDIIYELVTESNAIPSFCTGCYR
KGRTGDHFIGLAKQQFIGKFCQPNALITFKEYLNDYAGPKTRAAGTALIERELAAMSPARERNVRTCLAKTEAGERDIYL

Sequences:

>Translated_480_residues
MTTNEHPHSISISHYDANKSFIDEQAIWAAINNAASPSAEQVRQVLAKARQCQGLSIEETALLLQNQHEQLDEELFAVAR
EVKNTIYGNRIVLFAPLYVSNHCANSCSYCGFNADNHALKRKTLNQEEIRKEVTILEGMGHKRILAVYGEHPRNNVNAIV
DSIQTMYSVKDGKGGEIRRINVNCAPMSTADFKVLKTAAIGTYQCFQETYHQPTYESVHLKGKKKDYLYRLYAMHRAMEA
GIDDVGIGALFGLYDHRFELLAMLTHVAQLETVCGIGPHTISFPRIEPAHGSALSEKPPYEVDDACFKRIVAITRLAVPY
TGLIMSTRESAGLRKELLELGVSQISAGSRTSPGGYQDSEQDQHDAEQFSLGDHRAMDDIIYELVTESNAIPSFCTGCYR
KGRTGDHFIGLAKQQFIGKFCQPNALITFKEYLNDYAGPKTRAAGTALIERELAAMSPARERNVRTCLAKTEAGERDIYL
>Mature_479_residues
TTNEHPHSISISHYDANKSFIDEQAIWAAINNAASPSAEQVRQVLAKARQCQGLSIEETALLLQNQHEQLDEELFAVARE
VKNTIYGNRIVLFAPLYVSNHCANSCSYCGFNADNHALKRKTLNQEEIRKEVTILEGMGHKRILAVYGEHPRNNVNAIVD
SIQTMYSVKDGKGGEIRRINVNCAPMSTADFKVLKTAAIGTYQCFQETYHQPTYESVHLKGKKKDYLYRLYAMHRAMEAG
IDDVGIGALFGLYDHRFELLAMLTHVAQLETVCGIGPHTISFPRIEPAHGSALSEKPPYEVDDACFKRIVAITRLAVPYT
GLIMSTRESAGLRKELLELGVSQISAGSRTSPGGYQDSEQDQHDAEQFSLGDHRAMDDIIYELVTESNAIPSFCTGCYRK
GRTGDHFIGLAKQQFIGKFCQPNALITFKEYLNDYAGPKTRAAGTALIERELAAMSPARERNVRTCLAKTEAGERDIYL

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=353, Percent_Identity=30.8781869688385, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53461; Mature: 53330

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNEHPHSISISHYDANKSFIDEQAIWAAINNAASPSAEQVRQVLAKARQCQGLSIEET
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHH
ALLLQNQHEQLDEELFAVAREVKNTIYGNRIVLFAPLYVSNHCANSCSYCGFNADNHALK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCCCCCCCCCCCHHHH
RKTLNQEEIRKEVTILEGMGHKRILAVYGEHPRNNVNAIVDSIQTMYSVKDGKGGEIRRI
HHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
NVNCAPMSTADFKVLKTAAIGTYQCFQETYHQPTYESVHLKGKKKDYLYRLYAMHRAMEA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
GIDDVGIGALFGLYDHRFELLAMLTHVAQLETVCGIGPHTISFPRIEPAHGSALSEKPPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
EVDDACFKRIVAITRLAVPYTGLIMSTRESAGLRKELLELGVSQISAGSRTSPGGYQDSE
CCCHHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QDQHDAEQFSLGDHRAMDDIIYELVTESNAIPSFCTGCYRKGRTGDHFIGLAKQQFIGKF
CHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CQPNALITFKEYLNDYAGPKTRAAGTALIERELAAMSPARERNVRTCLAKTEAGERDIYL
CCCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTNEHPHSISISHYDANKSFIDEQAIWAAINNAASPSAEQVRQVLAKARQCQGLSIEET
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHH
ALLLQNQHEQLDEELFAVAREVKNTIYGNRIVLFAPLYVSNHCANSCSYCGFNADNHALK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCCCCCCCCCCCHHHH
RKTLNQEEIRKEVTILEGMGHKRILAVYGEHPRNNVNAIVDSIQTMYSVKDGKGGEIRRI
HHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
NVNCAPMSTADFKVLKTAAIGTYQCFQETYHQPTYESVHLKGKKKDYLYRLYAMHRAMEA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
GIDDVGIGALFGLYDHRFELLAMLTHVAQLETVCGIGPHTISFPRIEPAHGSALSEKPPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
EVDDACFKRIVAITRLAVPYTGLIMSTRESAGLRKELLELGVSQISAGSRTSPGGYQDSE
CCCHHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QDQHDAEQFSLGDHRAMDDIIYELVTESNAIPSFCTGCYRKGRTGDHFIGLAKQQFIGKF
CHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CQPNALITFKEYLNDYAGPKTRAAGTALIERELAAMSPARERNVRTCLAKTEAGERDIYL
CCCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]