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The map label for this gene is gshA

Identifier: 167623175

GI number: 167623175

Start: 1513579

End: 1515147

Strand: Direct

Name: gshA

Synonym: Shal_1241

Alternate gene names: 167623175

Gene position: 1513579-1515147 (Clockwise)

Preceding gene: 167623174

Following gene: 167623176

Centisome position: 28.96

GC content: 43.02

Gene sequence:

>1569_bases
TTGAAACCTTTCAATGAATTAGTAGGCAAGCTGTCGGATGCGGCAAGTCGTCAAGCGCTAAAATCAATGCACCGAGGTAT
TGAGCGTGAAGCGTTAAGAATTGAAAAGTCGGGTCACTTGGCGTTGGATAAGCATCCAAAAGCGCTAGGGTCGGCATTAA
TGCATTCGCGGATCACCACTGATTATAGCGAGTCTCTACTTGAGTTTATTACTCCTGTTTTTGAAGATATCGATGAATTA
GTCGAAGATCTGACCTTAACTCATGCCTATAGTGTGCGTCATTTAAACGGTCAACGCCTGTGGCCTGTTAGTATGCCGTG
TTACCTTGGGGACGCAGGCGATATTCCTATTGCTGATTATGGCAGCTCTAACACCGGACAAATGAAGCGTTTATATCGAA
AAGGGCTGACTTATCGATATGGTGCACAGATGCAAATTATTTCAGGAGTGCATTTTAATTTCTCTGTTTCAGACCAACTC
TGGAACAGATTGTACGAATTATCGGACACGTCTTTAAGTTTAGAGGAGTTTATCTCTGAGTCTTATTTCGGTCTTATTCG
TAACTATCGCCGTCTAGTCTGGGTGCTACCTTACCTGTTTGGTGCATCACCAGCGCTGTGCAGCACATTTATCCAAGATC
CAAAGACCAATGAGTTTCCTTTTGAAGTCATTGGTAACGGCACCTTGTATCTGCCTTATGCGACCTCATTGCGTATGAGC
GACTTAGGCTATACCAACCAAGAGCAAGACAACTTAAATATTAGTTACAATAGCTTAGCTTGTTACTTGGAAGGAATGAA
ATCAGCGATTAATATGCCTTCGGCTAAGTTCGCAAAAATTGGTGTCAAAGTTGATGGTGAGTATCGTCAGCTCAATGCCA
ATATTTTACAGATAGAAAATGAATTCTATTCCCCAATTCGTGCGAAACGTGTAGCAAAAGGTAACGAAAAGCCCTCAGAA
TCGCTTGCTCGTGCGGGGGTTGAGTATATTGAGGTTAGAGCATTAGATGTTAACCCTTATAGTGCTGTAGGTATTGAAAA
GTCACAAATTCGTTTCCTTGACTTGTTCTTATTGAATTGCTTATTGCAGCCATCACCAGCATCAGATGCAAGTGAAGAGG
CTGAGATTGCCGCTAACTTGCAAGCCGTGGTGTTAGAGGGACGCAAACCTGGGCTTAAATTAACTCGTGCAGGTGAAGAG
GTGAGTCTAAGTGGCTGGCTTGAGTCACTATTTGGTAATCTAAACGATATCGCTAAATTACTTGATGATGAAGGGCAAGA
CGATTACCAAGTCGCACTTGCTAAGTGGCAGAAGGCCGTCAACGATCCTGATGCAACTCTGTCGGGCCAAATTATTAAAG
GCCTTAAAGACAACCAAATTGATCATGGTGATTGGGTATTACAGCTTGCAGAGCAGTACCATCAAGAGCTAAAAGGCTAT
CCTTTATCAGAGGCTGTGACAATGCGCTATGAGCAGGACGCACAAGCATCACTTGAGAAACAAGCTAGGTTAGAAGCTGA
GCCTAGCGTGAGCTTTGATGATTTTTTAGCTGACTATTTTAAGGCATAA

Upstream 100 bases:

>100_bases
TAAAAAACGCTAATTTGGTACGACTTGAAATTTAAGTTGGCTATAGCGGGTCTGACTGACATGCTATATGGGTTAAAGTC
ACGTACTAGAGAGTAATATA

Downstream 100 bases:

>100_bases
CGTTGGGGACTCTGGGGTTGAAAAGCGTAGTGAGTGTTGCCGCATTGGCGTTGCTAATATCGGGCTGTGCGACACAACCC
GATACCGCTAATCAGTGCGG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKPFNELVGKLSDAASRQALKSMHRGIEREALRIEKSGHLALDKHPKALGSALMHSRITTDYSESLLEFITPVFEDIDEL
VEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDAGDIPIADYGSSNTGQMKRLYRKGLTYRYGAQMQIISGVHFNFSVSDQL
WNRLYELSDTSLSLEEFISESYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTNEFPFEVIGNGTLYLPYATSLRMS
DLGYTNQEQDNLNISYNSLACYLEGMKSAINMPSAKFAKIGVKVDGEYRQLNANILQIENEFYSPIRAKRVAKGNEKPSE
SLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSPASDASEEAEIAANLQAVVLEGRKPGLKLTRAGEE
VSLSGWLESLFGNLNDIAKLLDDEGQDDYQVALAKWQKAVNDPDATLSGQIIKGLKDNQIDHGDWVLQLAEQYHQELKGY
PLSEAVTMRYEQDAQASLEKQARLEAEPSVSFDDFLADYFKA

Sequences:

>Translated_522_residues
MKPFNELVGKLSDAASRQALKSMHRGIEREALRIEKSGHLALDKHPKALGSALMHSRITTDYSESLLEFITPVFEDIDEL
VEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDAGDIPIADYGSSNTGQMKRLYRKGLTYRYGAQMQIISGVHFNFSVSDQL
WNRLYELSDTSLSLEEFISESYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTNEFPFEVIGNGTLYLPYATSLRMS
DLGYTNQEQDNLNISYNSLACYLEGMKSAINMPSAKFAKIGVKVDGEYRQLNANILQIENEFYSPIRAKRVAKGNEKPSE
SLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSPASDASEEAEIAANLQAVVLEGRKPGLKLTRAGEE
VSLSGWLESLFGNLNDIAKLLDDEGQDDYQVALAKWQKAVNDPDATLSGQIIKGLKDNQIDHGDWVLQLAEQYHQELKGY
PLSEAVTMRYEQDAQASLEKQARLEAEPSVSFDDFLADYFKA
>Mature_522_residues
MKPFNELVGKLSDAASRQALKSMHRGIEREALRIEKSGHLALDKHPKALGSALMHSRITTDYSESLLEFITPVFEDIDEL
VEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDAGDIPIADYGSSNTGQMKRLYRKGLTYRYGAQMQIISGVHFNFSVSDQL
WNRLYELSDTSLSLEEFISESYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTNEFPFEVIGNGTLYLPYATSLRMS
DLGYTNQEQDNLNISYNSLACYLEGMKSAINMPSAKFAKIGVKVDGEYRQLNANILQIENEFYSPIRAKRVAKGNEKPSE
SLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSPASDASEEAEIAANLQAVVLEGRKPGLKLTRAGEE
VSLSGWLESLFGNLNDIAKLLDDEGQDDYQVALAKWQKAVNDPDATLSGQIIKGLKDNQIDHGDWVLQLAEQYHQELKGY
PLSEAVTMRYEQDAQASLEKQARLEAEPSVSFDDFLADYFKA

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=503, Percent_Identity=46.9184890656064, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_SHEHH (B0TK23)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673469.1
- ProteinModelPortal:   B0TK23
- SMR:   B0TK23
- GeneID:   5904229
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1241
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   PRK02107
- BioCyc:   SHAL458817:SHAL_1241-MONOMER
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 58584; Mature: 58584

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFNELVGKLSDAASRQALKSMHRGIEREALRIEKSGHLALDKHPKALGSALMHSRITT
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCEEECCCHHHHHHHHHHHHHCC
DYSESLLEFITPVFEDIDELVEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDAGDIPIADY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCEEECCCCCCCCCCC
GSSNTGQMKRLYRKGLTYRYGAQMQIISGVHFNFSVSDQLWNRLYELSDTSLSLEEFISE
CCCCHHHHHHHHHCCCCEEECCCEEEEECEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHH
SYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTNEFPFEVIGNGTLYLPYATSLRMS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCEEHH
DLGYTNQEQDNLNISYNSLACYLEGMKSAINMPSAKFAKIGVKVDGEYRQLNANILQIEN
HCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHCCHHEEECH
EFYSPIRAKRVAKGNEKPSESLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNC
HHHCHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LLQPSPASDASEEAEIAANLQAVVLEGRKPGLKLTRAGEEVSLSGWLESLFGNLNDIAKL
HCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHH
LDDEGQDDYQVALAKWQKAVNDPDATLSGQIIKGLKDNQIDHGDWVLQLAEQYHQELKGY
HCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
PLSEAVTMRYEQDAQASLEKQARLEAEPSVSFDDFLADYFKA
CCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKPFNELVGKLSDAASRQALKSMHRGIEREALRIEKSGHLALDKHPKALGSALMHSRITT
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCEEECCCHHHHHHHHHHHHHCC
DYSESLLEFITPVFEDIDELVEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDAGDIPIADY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCEEECCCCCCCCCCC
GSSNTGQMKRLYRKGLTYRYGAQMQIISGVHFNFSVSDQLWNRLYELSDTSLSLEEFISE
CCCCHHHHHHHHHCCCCEEECCCEEEEECEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHH
SYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTNEFPFEVIGNGTLYLPYATSLRMS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCEEHH
DLGYTNQEQDNLNISYNSLACYLEGMKSAINMPSAKFAKIGVKVDGEYRQLNANILQIEN
HCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHCCHHEEECH
EFYSPIRAKRVAKGNEKPSESLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNC
HHHCHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LLQPSPASDASEEAEIAANLQAVVLEGRKPGLKLTRAGEEVSLSGWLESLFGNLNDIAKL
HCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHH
LDDEGQDDYQVALAKWQKAVNDPDATLSGQIIKGLKDNQIDHGDWVLQLAEQYHQELKGY
HCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
PLSEAVTMRYEQDAQASLEKQARLEAEPSVSFDDFLADYFKA
CCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA