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The map label for this gene is eno

Identifier: 167623156

GI number: 167623156

Start: 1487068

End: 1488363

Strand: Direct

Name: eno

Synonym: Shal_1222

Alternate gene names: 167623156

Gene position: 1487068-1488363 (Clockwise)

Preceding gene: 167623155

Following gene: 167623157

Centisome position: 28.45

GC content: 45.37

Gene sequence:

>1296_bases
ATGGCTAAGATTATTAACATTATCGGTCGCGAAATCATGGATTCTCGCGGTAACCCAACTGTTGAAGCTGAAGTTCATTT
AGAAGGCGGATTCATGGGTATGGCTGCTGCACCGTCAGGCGCATCTACAGGTAGCCGTGAAGCACTAGAGCTTCGTGATG
GCGATAAAGCACGTTACATGGGTAAAGGTGTATTAAAAGCTGTTGAAAACATCAATGGACTTATCCGTGATGCTTTGATG
GGCAAAGATGCGACAGCGCAAGCTGAACTTGACCAAATCATGATTGACGTAGATGGTACTGAAAACAAAGATAAGCTAGG
CGCTAACGCGATTCTTGCTGTTTCTTTGGCTGCTGCTAAAGCGGCTGCAGCATTTAAAGGTGTTCCTTTATATGCGCATA
TCGCTGATCTAAATGGCACTCCAGGCCAGTACTCTATGCCTGTTCCTATGATGAACATCTTAAACGGTGGTGAGCACGCA
GATAACAATGTTGATATCCAAGAGTTCATGGTTCAACCTGTTGGCGCAAAGAGCTTCCGTGAAGCACTACGTATGGGCGC
TGAAATCTTCCATAGCCTTAAGAGCGTACTAAAGTCTAAAGGCTTAAGCACTTCTGTGGGTGATGAAGGTGGTTTCGCAC
CAGATCTAGCATCAAACGCTGACGCACTTGCAATCATCAAGGTTGCTGTAGAGAAAGCGGGTTACACGTTAGGTACTGAC
GTAACACTAGCGCTAGATTGCGCGGCATCTGAGTTTTACAAAGATGGTCAGTACGACCTATCTGGTGAAGGCAAAGTCTT
CTCGGCTAACGGTTTCTCTGACTTCCTTAAGTCGCTAACTGAACAGTACCCTATTGCTTCTATTGAAGATGGCTTAGATG
AGTCAGACTGGGATGGTTGGGCTTACCAGACTCAAATCATGGGTGACAAGATCCAGCTTGTTGGTGACGACTTGTTCGTA
ACTAACACTAAGATCTTGAAGCGTGGTATCGACAACGGTATTGCTAACTCAATCTTGATTAAGTTTAACCAAATCGGTTC
ACTAACAGAAACTTTAGCAGCGATTCGCATGGCGAAAGATGCGGGTTACACAGTAGTGATTTCACACCGTAGTGGTGAAA
CTGAAGATGCAACCATTGCCGATCTAGCAGTAGCGACTTCTGCTGGTCAAATCAAGACGGGTTCACTATGCCGTAGTGAC
CGTGTTGCTAAGTACAACCAGCTGCTTCGTATCGAAGAGCAGTTAGGCGAAAAAGCTCCTTATAACGGTCTTAAAGAGAT
CAAAGGTCAAGCATAA

Upstream 100 bases:

>100_bases
ATCGACCACTTTTTTATACCAATTGGTATCCGTTTGGATGAATAGAATTCTTTTTATTTTTATCTTTAAATTTATCTTTA
AAACTTAAATCGAGGGCATT

Downstream 100 bases:

>100_bases
ATCGCTATAAACTTTAAAAAGGCCACCACTCGGTGGCCTTTTTTGTTGTACTATCTGTTTAATATTCTCAATTTCTTTCT
ATTGATAATCATGAAACGCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAKIINIIGREIMDSRGNPTVEAEVHLEGGFMGMAAAPSGASTGSREALELRDGDKARYMGKGVLKAVENINGLIRDALM
GKDATAQAELDQIMIDVDGTENKDKLGANAILAVSLAAAKAAAAFKGVPLYAHIADLNGTPGQYSMPVPMMNILNGGEHA
DNNVDIQEFMVQPVGAKSFREALRMGAEIFHSLKSVLKSKGLSTSVGDEGGFAPDLASNADALAIIKVAVEKAGYTLGTD
VTLALDCAASEFYKDGQYDLSGEGKVFSANGFSDFLKSLTEQYPIASIEDGLDESDWDGWAYQTQIMGDKIQLVGDDLFV
TNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIRMAKDAGYTVVISHRSGETEDATIADLAVATSAGQIKTGSLCRSD
RVAKYNQLLRIEEQLGEKAPYNGLKEIKGQA

Sequences:

>Translated_431_residues
MAKIINIIGREIMDSRGNPTVEAEVHLEGGFMGMAAAPSGASTGSREALELRDGDKARYMGKGVLKAVENINGLIRDALM
GKDATAQAELDQIMIDVDGTENKDKLGANAILAVSLAAAKAAAAFKGVPLYAHIADLNGTPGQYSMPVPMMNILNGGEHA
DNNVDIQEFMVQPVGAKSFREALRMGAEIFHSLKSVLKSKGLSTSVGDEGGFAPDLASNADALAIIKVAVEKAGYTLGTD
VTLALDCAASEFYKDGQYDLSGEGKVFSANGFSDFLKSLTEQYPIASIEDGLDESDWDGWAYQTQIMGDKIQLVGDDLFV
TNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIRMAKDAGYTVVISHRSGETEDATIADLAVATSAGQIKTGSLCRSD
RVAKYNQLLRIEEQLGEKAPYNGLKEIKGQA
>Mature_430_residues
AKIINIIGREIMDSRGNPTVEAEVHLEGGFMGMAAAPSGASTGSREALELRDGDKARYMGKGVLKAVENINGLIRDALMG
KDATAQAELDQIMIDVDGTENKDKLGANAILAVSLAAAKAAAAFKGVPLYAHIADLNGTPGQYSMPVPMMNILNGGEHAD
NNVDIQEFMVQPVGAKSFREALRMGAEIFHSLKSVLKSKGLSTSVGDEGGFAPDLASNADALAIIKVAVEKAGYTLGTDV
TLALDCAASEFYKDGQYDLSGEGKVFSANGFSDFLKSLTEQYPIASIEDGLDESDWDGWAYQTQIMGDKIQLVGDDLFVT
NTKILKRGIDNGIANSILIKFNQIGSLTETLAAIRMAKDAGYTVVISHRSGETEDATIADLAVATSAGQIKTGSLCRSDR
VAKYNQLLRIEEQLGEKAPYNGLKEIKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=53.5046728971963, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=53.5046728971963, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=53.8461538461538, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=51.981351981352, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=48.3568075117371, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=25.5952380952381, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=25.5952380952381, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=25.5952380952381, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=79.3981481481482, Blast_Score=698, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=53.8283062645012, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=53.8283062645012, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=50, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.9607390300231, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=49.6519721577726, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.729792147806, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.729792147806, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.9559164733179, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=427, Percent_Identity=52.2248243559719, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=427, Percent_Identity=52.2248243559719, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=427, Percent_Identity=52.2248243559719, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=427, Percent_Identity=52.2248243559719, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=427, Percent_Identity=52.2248243559719, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=427, Percent_Identity=52.2248243559719, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_SHEHH (B0TK04)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673450.1
- ProteinModelPortal:   B0TK04
- SMR:   B0TK04
- GeneID:   5903912
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1222
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   SHAL458817:SHAL_1222-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45737; Mature: 45606

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 341-341 BINDING 159-159 BINDING 168-168 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIINIIGREIMDSRGNPTVEAEVHLEGGFMGMAAAPSGASTGSREALELRDGDKARYM
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVENINGLIRDALMGKDATAQAELDQIMIDVDGTENKDKLGANAILAVSLAAAK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEECCCCCCCHHHCCCCEEEHHHHHHH
AAAAFKGVPLYAHIADLNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVGAKSFR
HHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRMGAEIFHSLKSVLKSKGLSTSVGDEGGFAPDLASNADALAIIKVAVEKAGYTLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCC
VTLALDCAASEFYKDGQYDLSGEGKVFSANGFSDFLKSLTEQYPIASIEDGLDESDWDGW
EEEEEEHHHHHHHCCCCEEECCCCCEEECCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCE
AYQTQIMGDKIQLVGDDLFVTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIRMAKD
EEEEEECCCEEEEECCCEEEECHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHCC
AGYTVVISHRSGETEDATIADLAVATSAGQIKTGSLCRSDRVAKYNQLLRIEEQLGEKAP
CCCEEEEECCCCCCCCCHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
YNGLKEIKGQA
CCCHHHHCCCC
>Mature Secondary Structure 
AKIINIIGREIMDSRGNPTVEAEVHLEGGFMGMAAAPSGASTGSREALELRDGDKARYM
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVENINGLIRDALMGKDATAQAELDQIMIDVDGTENKDKLGANAILAVSLAAAK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEECCCCCCCHHHCCCCEEEHHHHHHH
AAAAFKGVPLYAHIADLNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVGAKSFR
HHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRMGAEIFHSLKSVLKSKGLSTSVGDEGGFAPDLASNADALAIIKVAVEKAGYTLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCC
VTLALDCAASEFYKDGQYDLSGEGKVFSANGFSDFLKSLTEQYPIASIEDGLDESDWDGW
EEEEEEHHHHHHHCCCCEEECCCCCEEECCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCE
AYQTQIMGDKIQLVGDDLFVTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIRMAKD
EEEEEECCCEEEEECCCEEEECHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHCC
AGYTVVISHRSGETEDATIADLAVATSAGQIKTGSLCRSDRVAKYNQLLRIEEQLGEKAP
CCCEEEEECCCCCCCCCHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
YNGLKEIKGQA
CCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA