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The map label for this gene is thrA [H]

Identifier: 167623048

GI number: 167623048

Start: 1352672

End: 1355137

Strand: Direct

Name: thrA [H]

Synonym: Shal_1114

Alternate gene names: 167623048

Gene position: 1352672-1355137 (Clockwise)

Preceding gene: 167623045

Following gene: 167623049

Centisome position: 25.88

GC content: 47.12

Gene sequence:

>2466_bases
ATGAAAGTAATGAAATTTGGTGGAACCTCGCTTGCTAATTGGCAGCGTTTTAAAGGCGCTGCGGAGATTATCCGTCAGTC
TGCTAATTCACAAGCTACAGCCGTAGTATTATCAGCCCCTGCCACCGTTACCAACGGCTTGATTGAGATGGTTGATGTTG
CAGTAAAAGGGGGGGACTACTGTGCCGTGCTAGCCAAGGTAGAACAGGTTTTTAAAGACCTTTATGCAGCAGCGGCGGTT
AGCTTAAGTACAGAACAAAATATTTTGCTTAATGAGAAGCTACAGCAGCAGCTAACTTTTTGGCAGAGTAAGCTTGACGG
AATGAGCTTACTTGGCGAGTGCCCTGAAAGTGTTGAAGCACAGGTTTTGGTCGGTGGGGAGAAGCTGTCATCGGCGTTAA
TGGTTGAAGTGATGAAGTCTTTTAACCATAGTGCGGATTTATTAGTGCCCGAGTCTTTACTCGTTGGTTATGGCCCACGT
CTTGAGTCTGCAGTCGATATTGAAGCAAGTAAACTTCGCTTCAATGCTCTAGATTTATCTGTGGCAAAAGTATGGGTGAT
GCCAGGGTTTACAGCGGGTGACAGTGAAGGCAAGGTGGTCACACTCGGACGTAATGGCTCTGATTATTCTGCGGCAGTAT
TATCAGCTTGTATCGGCGCAAGCTGTTGTGAGATCTGGACCGATGTTGATGGTGTCTATAATACCGATCCGCGAGTGGTT
GCAGATGCCAAACTTTTGTCTCAGCTTAGTTATCAAGAGGCGATGGAGGTATCTTACTTTGGTGCAAAGGTGTTACATCC
TAAGACTGTAGCGCCTATCGCGCAGTACCATATTCCGTGTTATATCCGTAATACCTTCAATCCAGAAGCCGTGGGCACAT
TGGTGTCAGATCAGCCCGATGAAACAGGGCTCAATGTCAAAGCCATTTCTAATCTAGACTCGCAAACGATGTTTGATGTA
TCAGGCCCTGGCATGAAAGGCATGGTCGGTATGGCGAGTCGTACACTTGGCGCTATTTCTCGCTCTGGTGTGTCGGTGTC
CTTGATCACTCAAAGTTCCTCTGAATACAGCATCAGTTTCTGTGTCGCTACTGAAGACGCAATTAAAGCCAAGTTTGCCC
TAGAGCAAGAGTTTGAACTTGAGCTTAAAAGCGACATTCTAGAGCCGATTGAGATGCGTCATGACTTAGCAATTGTCTCA
CTTATTGGCGATGGTATGCGCACCCACAAAGGTGTGGCCGCCAAATTCTTCCAGGCGTTAGCTCAGGCGACAGTAAATAT
CGTGGCTATCGCCCAGGGCTCATCTGAGCGTTCGATCTCTGCGGTGGTAGAGCAGCGCAAGACTAAGCATGCTATCGCTG
CCTGTCATCAATCTTTCTTTGATGTGCAGCATTACCTAGATGTATTTCTTGTCGGCTGCGGTAATGTGGGCGCCGGTTTA
CTTGAGCAGATAAAGCTGCAGCGTAGCATGTTAAAAGAGCAACATATTGCTATCCGAGTCTGCGGGATCGTTAATTCGAA
AAAAATGTTACTCGATGCACAAGGTATCGACTTAGCCAACTGGCAGGGCGTATTAGCCGATTGTGAGCAAGAGGCGGATC
TCGATGCTATGTTGGTATGGGCGAAGGAGCAGCAGCTACTCAACCCTGTGCTGGTGGACTGCACCTCGAGTGAATTGGTA
TCGAATAAATACCTAGATGTTATGAATGCAGGGCTTCATGTTGTTACGCCAAATAAGAAGGCTAACACTCGCGATATGGC
GTATTACCAAGATTTAAGACAAACAGCGCTTAAGCAGCGTCGTCAGTTCCTGTATGAGACAACCGTTGGTGCGGGTCTGC
CTGTTATCGATAACCTAAAAAAACTACTATTTGCTGGTGATAAGTTGCTGCGCTTTAATGGCATTTTATCGGGCAGTTTG
TCTTACATTTTCGGCATGCTTGATGAAGGCATGACTCTGTCCCAAGCAACATCAATCGCCAGAGATAAATGCTTTACCGA
GCCCGATCCTCGTGATGATTTAAGTGGTATGGACGTGGCTCGTAAAGTGCTTATTCTTGCAAGGGAAGTGGGCATGGAGC
TTGAGCTTAGCGATATCCATGTTGAATCTGTATTACCTGATTCATTCGATGCCTCTGGCGACGTTAATGCCTTTATGGCT
AAACTGCCGTCGTTAGATGCAGAGGTTGCCCTGCGAGTTGAAGCCGCTAAAGGACAAGGTAAAGTGCTGCGCTATGTTGG
GCAGATTGATGATGAAGGCTGTAAGGTTAAAATAGTCGAAGTCGGTGCCAATGATCCGCTATACAGCGTTAAAGGGGGAG
AGAATGCCCTCGCATTTTATAGCCGCTATTACCAGCCTATCCCATTTGTACTTCGAGGATATGGTGCGGGTACCGATGTT
ACTGCTGCGGGCGCATTTGCCGATATTTTACGTACACTAAATTGGACTCGTGAGGTGGGGGTATGA

Upstream 100 bases:

>100_bases
CGGGCTAAGCACAACCTGATATAAGTAAAATATCAAGCCCGCTCCCCCAAAAGGAGCGGGCTTTTTTGTTTCTTAGGTGG
CGCGGCACAAGGAGATTTTG

Downstream 100 bases:

>100_bases
GTGTAACCATTTATGCCCCAGCATCGATGGGCAACGTAGGCGTGGGTTTTGACCTACTGGGGGCGGCGTTAGCCCCTATT
GATGGCAGTTTGCTTGGCGA

Product: bifunctional aspartokinase I/homoserine dehydrogenase I

Products: NA

Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 821; Mature: 821

Protein sequence:

>821_residues
MKVMKFGGTSLANWQRFKGAAEIIRQSANSQATAVVLSAPATVTNGLIEMVDVAVKGGDYCAVLAKVEQVFKDLYAAAAV
SLSTEQNILLNEKLQQQLTFWQSKLDGMSLLGECPESVEAQVLVGGEKLSSALMVEVMKSFNHSADLLVPESLLVGYGPR
LESAVDIEASKLRFNALDLSVAKVWVMPGFTAGDSEGKVVTLGRNGSDYSAAVLSACIGASCCEIWTDVDGVYNTDPRVV
ADAKLLSQLSYQEAMEVSYFGAKVLHPKTVAPIAQYHIPCYIRNTFNPEAVGTLVSDQPDETGLNVKAISNLDSQTMFDV
SGPGMKGMVGMASRTLGAISRSGVSVSLITQSSSEYSISFCVATEDAIKAKFALEQEFELELKSDILEPIEMRHDLAIVS
LIGDGMRTHKGVAAKFFQALAQATVNIVAIAQGSSERSISAVVEQRKTKHAIAACHQSFFDVQHYLDVFLVGCGNVGAGL
LEQIKLQRSMLKEQHIAIRVCGIVNSKKMLLDAQGIDLANWQGVLADCEQEADLDAMLVWAKEQQLLNPVLVDCTSSELV
SNKYLDVMNAGLHVVTPNKKANTRDMAYYQDLRQTALKQRRQFLYETTVGAGLPVIDNLKKLLFAGDKLLRFNGILSGSL
SYIFGMLDEGMTLSQATSIARDKCFTEPDPRDDLSGMDVARKVLILAREVGMELELSDIHVESVLPDSFDASGDVNAFMA
KLPSLDAEVALRVEAAKGQGKVLRYVGQIDDEGCKVKIVEVGANDPLYSVKGGENALAFYSRYYQPIPFVLRGYGAGTDV
TAAGAFADILRTLNWTREVGV

Sequences:

>Translated_821_residues
MKVMKFGGTSLANWQRFKGAAEIIRQSANSQATAVVLSAPATVTNGLIEMVDVAVKGGDYCAVLAKVEQVFKDLYAAAAV
SLSTEQNILLNEKLQQQLTFWQSKLDGMSLLGECPESVEAQVLVGGEKLSSALMVEVMKSFNHSADLLVPESLLVGYGPR
LESAVDIEASKLRFNALDLSVAKVWVMPGFTAGDSEGKVVTLGRNGSDYSAAVLSACIGASCCEIWTDVDGVYNTDPRVV
ADAKLLSQLSYQEAMEVSYFGAKVLHPKTVAPIAQYHIPCYIRNTFNPEAVGTLVSDQPDETGLNVKAISNLDSQTMFDV
SGPGMKGMVGMASRTLGAISRSGVSVSLITQSSSEYSISFCVATEDAIKAKFALEQEFELELKSDILEPIEMRHDLAIVS
LIGDGMRTHKGVAAKFFQALAQATVNIVAIAQGSSERSISAVVEQRKTKHAIAACHQSFFDVQHYLDVFLVGCGNVGAGL
LEQIKLQRSMLKEQHIAIRVCGIVNSKKMLLDAQGIDLANWQGVLADCEQEADLDAMLVWAKEQQLLNPVLVDCTSSELV
SNKYLDVMNAGLHVVTPNKKANTRDMAYYQDLRQTALKQRRQFLYETTVGAGLPVIDNLKKLLFAGDKLLRFNGILSGSL
SYIFGMLDEGMTLSQATSIARDKCFTEPDPRDDLSGMDVARKVLILAREVGMELELSDIHVESVLPDSFDASGDVNAFMA
KLPSLDAEVALRVEAAKGQGKVLRYVGQIDDEGCKVKIVEVGANDPLYSVKGGENALAFYSRYYQPIPFVLRGYGAGTDV
TAAGAFADILRTLNWTREVGV
>Mature_821_residues
MKVMKFGGTSLANWQRFKGAAEIIRQSANSQATAVVLSAPATVTNGLIEMVDVAVKGGDYCAVLAKVEQVFKDLYAAAAV
SLSTEQNILLNEKLQQQLTFWQSKLDGMSLLGECPESVEAQVLVGGEKLSSALMVEVMKSFNHSADLLVPESLLVGYGPR
LESAVDIEASKLRFNALDLSVAKVWVMPGFTAGDSEGKVVTLGRNGSDYSAAVLSACIGASCCEIWTDVDGVYNTDPRVV
ADAKLLSQLSYQEAMEVSYFGAKVLHPKTVAPIAQYHIPCYIRNTFNPEAVGTLVSDQPDETGLNVKAISNLDSQTMFDV
SGPGMKGMVGMASRTLGAISRSGVSVSLITQSSSEYSISFCVATEDAIKAKFALEQEFELELKSDILEPIEMRHDLAIVS
LIGDGMRTHKGVAAKFFQALAQATVNIVAIAQGSSERSISAVVEQRKTKHAIAACHQSFFDVQHYLDVFLVGCGNVGAGL
LEQIKLQRSMLKEQHIAIRVCGIVNSKKMLLDAQGIDLANWQGVLADCEQEADLDAMLVWAKEQQLLNPVLVDCTSSELV
SNKYLDVMNAGLHVVTPNKKANTRDMAYYQDLRQTALKQRRQFLYETTVGAGLPVIDNLKKLLFAGDKLLRFNGILSGSL
SYIFGMLDEGMTLSQATSIARDKCFTEPDPRDDLSGMDVARKVLILAREVGMELELSDIHVESVLPDSFDASGDVNAFMA
KLPSLDAEVALRVEAAKGQGKVLRYVGQIDDEGCKVKIVEVGANDPLYSVKGGENALAFYSRYYQPIPFVLRGYGAGTDV
TAAGAFADILRTLNWTREVGV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=816, Percent_Identity=56.3725490196078, Blast_Score=926, Evalue=0.0,
Organism=Escherichia coli, GI1790376, Length=820, Percent_Identity=30.3658536585366, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1790455, Length=468, Percent_Identity=28.4188034188034, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322599, Length=336, Percent_Identity=31.8452380952381, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320893, Length=498, Percent_Identity=24.4979919678715, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88815; Mature: 88815

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMKFGGTSLANWQRFKGAAEIIRQSANSQATAVVLSAPATVTNGLIEMVDVAVKGGDY
CCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCH
CAVLAKVEQVFKDLYAAAAVSLSTEQNILLNEKLQQQLTFWQSKLDGMSLLGECPESVEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCE
QVLVGGEKLSSALMVEVMKSFNHSADLLVPESLLVGYGPRLESAVDIEASKLRFNALDLS
EEEECHHHHHHHHHHHHHHHCCCCCCEEECHHHHHCCCCCCCCCCCCCHHHEEEEEEEEE
VAKVWVMPGFTAGDSEGKVVTLGRNGSDYSAAVLSACIGASCCEIWTDVDGVYNTDPRVV
EEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCEE
ADAKLLSQLSYQEAMEVSYFGAKVLHPKTVAPIAQYHIPCYIRNTFNPEAVGTLVSDQPD
HHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCC
ETGLNVKAISNLDSQTMFDVSGPGMKGMVGMASRTLGAISRSGVSVSLITQSSSEYSISF
CCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
CVATEDAIKAKFALEQEFELELKSDILEPIEMRHDLAIVSLIGDGMRTHKGVAAKFFQAL
EEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCHHHHHHHHHHHHHHH
AQATVNIVAIAQGSSERSISAVVEQRKTKHAIAACHQSFFDVQHYLDVFLVGCGNVGAGL
HHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
LEQIKLQRSMLKEQHIAIRVCGIVNSKKMLLDAQGIDLANWQGVLADCEQEADLDAMLVW
HHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEE
AKEQQLLNPVLVDCTSSELVSNKYLDVMNAGLHVVTPNKKANTRDMAYYQDLRQTALKQR
EHHHHHCCCCEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
RQFLYETTVGAGLPVIDNLKKLLFAGDKLLRFNGILSGSLSYIFGMLDEGMTLSQATSIA
HHHHHHHHCCCCCCHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHCCCCCHHHHHHHH
RDKCFTEPDPRDDLSGMDVARKVLILAREVGMELELSDIHVESVLPDSFDASGDVNAFMA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCEEHHHCCCCCCCCCCCHHHHHH
KLPSLDAEVALRVEAAKGQGKVLRYVGQIDDEGCKVKIVEVGANDPLYSVKGGENALAFY
HCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCHHHHH
SRYYQPIPFVLRGYGAGTDVTAAGAFADILRTLNWTREVGV
HHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKVMKFGGTSLANWQRFKGAAEIIRQSANSQATAVVLSAPATVTNGLIEMVDVAVKGGDY
CCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCH
CAVLAKVEQVFKDLYAAAAVSLSTEQNILLNEKLQQQLTFWQSKLDGMSLLGECPESVEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCE
QVLVGGEKLSSALMVEVMKSFNHSADLLVPESLLVGYGPRLESAVDIEASKLRFNALDLS
EEEECHHHHHHHHHHHHHHHCCCCCCEEECHHHHHCCCCCCCCCCCCCHHHEEEEEEEEE
VAKVWVMPGFTAGDSEGKVVTLGRNGSDYSAAVLSACIGASCCEIWTDVDGVYNTDPRVV
EEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCEE
ADAKLLSQLSYQEAMEVSYFGAKVLHPKTVAPIAQYHIPCYIRNTFNPEAVGTLVSDQPD
HHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCC
ETGLNVKAISNLDSQTMFDVSGPGMKGMVGMASRTLGAISRSGVSVSLITQSSSEYSISF
CCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
CVATEDAIKAKFALEQEFELELKSDILEPIEMRHDLAIVSLIGDGMRTHKGVAAKFFQAL
EEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCHHHHHHHHHHHHHHH
AQATVNIVAIAQGSSERSISAVVEQRKTKHAIAACHQSFFDVQHYLDVFLVGCGNVGAGL
HHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
LEQIKLQRSMLKEQHIAIRVCGIVNSKKMLLDAQGIDLANWQGVLADCEQEADLDAMLVW
HHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEE
AKEQQLLNPVLVDCTSSELVSNKYLDVMNAGLHVVTPNKKANTRDMAYYQDLRQTALKQR
EHHHHHCCCCEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
RQFLYETTVGAGLPVIDNLKKLLFAGDKLLRFNGILSGSLSYIFGMLDEGMTLSQATSIA
HHHHHHHHCCCCCCHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHCCCCCHHHHHHHH
RDKCFTEPDPRDDLSGMDVARKVLILAREVGMELELSDIHVESVLPDSFDASGDVNAFMA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCEEHHHCCCCCCCCCCCHHHHHH
KLPSLDAEVALRVEAAKGQGKVLRYVGQIDDEGCKVKIVEVGANDPLYSVKGGENALAFY
HCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCHHHHH
SRYYQPIPFVLRGYGAGTDVTAAGAFADILRTLNWTREVGV
HHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7003595; 1630901; 7610040; 9278503; 6277952; 2410621; 387092; 6298218; 390305 [H]