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The map label for this gene is ptsP [H]

Identifier: 167623002

GI number: 167623002

Start: 1306314

End: 1308548

Strand: Direct

Name: ptsP [H]

Synonym: Shal_1068

Alternate gene names: 167623002

Gene position: 1306314-1308548 (Clockwise)

Preceding gene: 167623001

Following gene: 167623003

Centisome position: 24.99

GC content: 45.59

Gene sequence:

>2235_bases
GTGCTAAAAACACTGAGAGATATTACACAGGCAGTAGCGTCTTCACGCGATCTTCAATACGCGTTAGAGCTACTTGTTAC
GCAAACCAAACAAGCTATGGCTACAGAGTGTTGCTCTATCTATATATTAGAGGAGCAACAACTCGTTCTTTCTGCAACGG
ACGGCCTAGACCCTGCGGCTGTTAATCGTGTTAAACTCCCCGTTACACAAGGCTTAGTTGGCTTAGTTGCTCAGCGAGAA
GAGGCAATTAACTTAGCCGATGCACCATCCCACCCTAGATTTAAACTACTCCCTGAAGTGGCTGAAGAAGCTTATAGTGC
TTTTCTCGCAGTGCCAATCGTATATCAAAAGTCGGTTATTGGCGTTATCGTGGTTCAGCAAGTTCAAGCTCGTCAGTTTA
GTGAGGGAGAAGAAGCCTTCTTAATGACGCTAGCCGCTCAGTTGGCTGTTGCAATTAAAGGGCTAACCCATAGAGCAAAG
TCTAGCAGTAGCCTACAGCAGCGATATTATACTGGCACTCAAGCTTCAAAAGGCATTGCGATTGCAAAAGCTCTGATGTT
GGGGGGGCAGATCTCGTTTGATCTTGCTTTGCAGCGTGCAAACTCAATAGAAGAGGAGGAGAAACGCCTTCAAGATGCGA
TGAACCGCTGCAAAGATTTGCTCGTCGGTCTATCTCAGCGATTCGACAGGGAGAAAGATGAAGAAGCCGCATCGATTTTT
AAAGCGCTGTTGTTATTGCTTGAGAAGGCCAGTTTGGGCGGTGAATACATTGAAGAGGTTAGAGCGGGTTGGAGTTCTGA
AACCGCTGTTTGTCAGGTATCTAAGCGGTACATAGAACACTTTGGCTCGATGAGTGACCTTTATCTAAAAGAGCGAGCTA
ATGATATTAGAGATCTCGGTCAGCGCGTTCTTAGGCAGTTGATCGAACCTGATAAGATTTTTTTAGAGCCAGAAACACCG
GTGATCTTGATTACTCGTGAAGCCAGTACAACTCTGTTAGCAGAATTTCCTAGACAAAAGTTGGCGGGGATCGTAACCGA
GCTTGGCGGCGCCAACTCTCATGCGGCTATTTTAGCTAGGGCGATGGGGGTACCTGCAATTATTGGTGTCGAAGGTATTT
TAAGCGCTAATATCGAAAAGTCGCAGATCATTATCAATGCCAATCGTGGTCAGGTTATCATTGAACCTTCAGCGTTAGTA
TTATCTGAGTATCGAAGCTTAATTGCGGCGCAAGAAGAGCAACAAAGACAATATGCGCAAGAGTTATCTCTTAAAGCGGT
TACCCTCGATAACAAGCGCATTTATCTCTACATCAATAGTGGATTGTTGAGTAGTGTCGACTCTGAAACCGGTGCCGACT
CTGATGGGATTGGGCTCTATCGCACCGAGATCCTGTTTATGCTCAAACAGTGCTTCCCTGGAGAAATAGAACAGCTAGAA
ATTTATCGCAAGGTTTTAAAGGCGGCGGGTAATAAGCCTGTGGTGATGCGAACCCTTGATGTTGGAGGGGATAAACCGCT
CGATTACTTCCCTATTATTGAAGATAACCCGTTTTTGGGCTGGCGTGGCATACGATTATCGCTGGATCATCCTGAGTTAT
TCCTCGTTCAGCTTAGAGCAATGTTACAGGCGGCTATTGACACTGAGCAGCTGCATATATTACTGCCGATGGTCAGTAAT
CTTGAAGAGATAGATTTATCATTGATCTATTTAGAGCAAGCTTGTCAAGAGCTGACTTCTGAGCTTGGCAAGAGCATTAC
TATGCCTAAAGTGGGTATTATGCTCGAAGTACCAGTATTGTTGTATCAGCTAGATGAAGTGGCTAAACGTGTGGATTTTG
TCTCTGTGGGATCGAACGATCTGACTCAGTACTTATTGGCTGTCGACCGCAATAACCCCAGTGTTAGCCCATTATTTGAT
AGCTATCACCCTGGCGTACTCCGAGCGCTTAAATTTGCCGTCGAACGCTGTGGCGAGCATCAATTGCCGATTAGTGTCTG
TGGGGAATTAGCGGGTGAGCCGATTGGCGCCATGTTACTTGTCGCAATGGGCTATGATCAACTTAGTATGAACCAAGCAA
GCTTAGCGCGGATTAATTATCTGTTAAGGCGTGTTTCCCAAACAGAGTTGAATCAGTTACTGGGTGAGGCGTTAAAGCTT
AATAGTGGCGAAGAGGTGAGGTTGCTAGTGGCTCAATACTGTGAGCAAAAGGGTCTTGCGTCAGTATTAAGTTAA

Upstream 100 bases:

>100_bases
ATGAAAGAGTTCGCTGCGACCACTTTGCCTTTGCAGGTTAAAGAGTCGAATAACAGAAGACGAGGTATGCGCAGAAGCTA
AATAAGGGAGGTTTGCGGTA

Downstream 100 bases:

>100_bases
AAGGGCTTATGCTCTATGAACTCCATCTTGATTGTTTTAAGCTTGATAAATAATAACTACTAAAGGTAGCGCTTGTGGAA
AGCTGGCTATATATATTGTG

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MLKTLRDITQAVASSRDLQYALELLVTQTKQAMATECCSIYILEEQQLVLSATDGLDPAAVNRVKLPVTQGLVGLVAQRE
EAINLADAPSHPRFKLLPEVAEEAYSAFLAVPIVYQKSVIGVIVVQQVQARQFSEGEEAFLMTLAAQLAVAIKGLTHRAK
SSSSLQQRYYTGTQASKGIAIAKALMLGGQISFDLALQRANSIEEEEKRLQDAMNRCKDLLVGLSQRFDREKDEEAASIF
KALLLLLEKASLGGEYIEEVRAGWSSETAVCQVSKRYIEHFGSMSDLYLKERANDIRDLGQRVLRQLIEPDKIFLEPETP
VILITREASTTLLAEFPRQKLAGIVTELGGANSHAAILARAMGVPAIIGVEGILSANIEKSQIIINANRGQVIIEPSALV
LSEYRSLIAAQEEQQRQYAQELSLKAVTLDNKRIYLYINSGLLSSVDSETGADSDGIGLYRTEILFMLKQCFPGEIEQLE
IYRKVLKAAGNKPVVMRTLDVGGDKPLDYFPIIEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAAIDTEQLHILLPMVSN
LEEIDLSLIYLEQACQELTSELGKSITMPKVGIMLEVPVLLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPSVSPLFD
SYHPGVLRALKFAVERCGEHQLPISVCGELAGEPIGAMLLVAMGYDQLSMNQASLARINYLLRRVSQTELNQLLGEALKL
NSGEEVRLLVAQYCEQKGLASVLS

Sequences:

>Translated_744_residues
MLKTLRDITQAVASSRDLQYALELLVTQTKQAMATECCSIYILEEQQLVLSATDGLDPAAVNRVKLPVTQGLVGLVAQRE
EAINLADAPSHPRFKLLPEVAEEAYSAFLAVPIVYQKSVIGVIVVQQVQARQFSEGEEAFLMTLAAQLAVAIKGLTHRAK
SSSSLQQRYYTGTQASKGIAIAKALMLGGQISFDLALQRANSIEEEEKRLQDAMNRCKDLLVGLSQRFDREKDEEAASIF
KALLLLLEKASLGGEYIEEVRAGWSSETAVCQVSKRYIEHFGSMSDLYLKERANDIRDLGQRVLRQLIEPDKIFLEPETP
VILITREASTTLLAEFPRQKLAGIVTELGGANSHAAILARAMGVPAIIGVEGILSANIEKSQIIINANRGQVIIEPSALV
LSEYRSLIAAQEEQQRQYAQELSLKAVTLDNKRIYLYINSGLLSSVDSETGADSDGIGLYRTEILFMLKQCFPGEIEQLE
IYRKVLKAAGNKPVVMRTLDVGGDKPLDYFPIIEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAAIDTEQLHILLPMVSN
LEEIDLSLIYLEQACQELTSELGKSITMPKVGIMLEVPVLLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPSVSPLFD
SYHPGVLRALKFAVERCGEHQLPISVCGELAGEPIGAMLLVAMGYDQLSMNQASLARINYLLRRVSQTELNQLLGEALKL
NSGEEVRLLVAQYCEQKGLASVLS
>Mature_744_residues
MLKTLRDITQAVASSRDLQYALELLVTQTKQAMATECCSIYILEEQQLVLSATDGLDPAAVNRVKLPVTQGLVGLVAQRE
EAINLADAPSHPRFKLLPEVAEEAYSAFLAVPIVYQKSVIGVIVVQQVQARQFSEGEEAFLMTLAAQLAVAIKGLTHRAK
SSSSLQQRYYTGTQASKGIAIAKALMLGGQISFDLALQRANSIEEEEKRLQDAMNRCKDLLVGLSQRFDREKDEEAASIF
KALLLLLEKASLGGEYIEEVRAGWSSETAVCQVSKRYIEHFGSMSDLYLKERANDIRDLGQRVLRQLIEPDKIFLEPETP
VILITREASTTLLAEFPRQKLAGIVTELGGANSHAAILARAMGVPAIIGVEGILSANIEKSQIIINANRGQVIIEPSALV
LSEYRSLIAAQEEQQRQYAQELSLKAVTLDNKRIYLYINSGLLSSVDSETGADSDGIGLYRTEILFMLKQCFPGEIEQLE
IYRKVLKAAGNKPVVMRTLDVGGDKPLDYFPIIEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAAIDTEQLHILLPMVSN
LEEIDLSLIYLEQACQELTSELGKSITMPKVGIMLEVPVLLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPSVSPLFD
SYHPGVLRALKFAVERCGEHQLPISVCGELAGEPIGAMLLVAMGYDQLSMNQASLARINYLLRRVSQTELNQLLGEALKL
NSGEEVRLLVAQYCEQKGLASVLS

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=754, Percent_Identity=41.9098143236074, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=33.7391304347826, Blast_Score=299, Evalue=5e-82,
Organism=Escherichia coli, GI48994992, Length=510, Percent_Identity=34.7058823529412, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788726, Length=523, Percent_Identity=33.0783938814532, Blast_Score=259, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82103; Mature: 82103

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTLRDITQAVASSRDLQYALELLVTQTKQAMATECCSIYILEEQQLVLSATDGLDPAA
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEECCCCCHHH
VNRVKLPVTQGLVGLVAQREEAINLADAPSHPRFKLLPEVAEEAYSAFLAVPIVYQKSVI
HCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVIVVQQVQARQFSEGEEAFLMTLAAQLAVAIKGLTHRAKSSSSLQQRYYTGTQASKGIA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHH
IAKALMLGGQISFDLALQRANSIEEEEKRLQDAMNRCKDLLVGLSQRFDREKDEEAASIF
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KALLLLLEKASLGGEYIEEVRAGWSSETAVCQVSKRYIEHFGSMSDLYLKERANDIRDLG
HHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QRVLRQLIEPDKIFLEPETPVILITREASTTLLAEFPRQKLAGIVTELGGANSHAAILAR
HHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMGVPAIIGVEGILSANIEKSQIIINANRGQVIIEPSALVLSEYRSLIAAQEEQQRQYAQ
HHCCCEEECCHHHHHCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
ELSLKAVTLDNKRIYLYINSGLLSSVDSETGADSDGIGLYRTEILFMLKQCFPGEIEQLE
HCCEEEEEECCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
IYRKVLKAAGNKPVVMRTLDVGGDKPLDYFPIIEDNPFLGWRGIRLSLDHPELFLVQLRA
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MLQAAIDTEQLHILLPMVSNLEEIDLSLIYLEQACQELTSELGKSITMPKVGIMLEVPVL
HHHHHCCHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHH
LYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPSVSPLFDSYHPGVLRALKFAVERCGEH
HHHHHHHHHHHCEEECCCHHHHHHHHHEECCCCCCCCCHHCCCHHHHHHHHHHHHHCCCC
QLPISVCGELAGEPIGAMLLVAMGYDQLSMNQASLARINYLLRRVSQTELNQLLGEALKL
CCCHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NSGEEVRLLVAQYCEQKGLASVLS
CCCCHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MLKTLRDITQAVASSRDLQYALELLVTQTKQAMATECCSIYILEEQQLVLSATDGLDPAA
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEECCCCCHHH
VNRVKLPVTQGLVGLVAQREEAINLADAPSHPRFKLLPEVAEEAYSAFLAVPIVYQKSVI
HCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVIVVQQVQARQFSEGEEAFLMTLAAQLAVAIKGLTHRAKSSSSLQQRYYTGTQASKGIA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHH
IAKALMLGGQISFDLALQRANSIEEEEKRLQDAMNRCKDLLVGLSQRFDREKDEEAASIF
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KALLLLLEKASLGGEYIEEVRAGWSSETAVCQVSKRYIEHFGSMSDLYLKERANDIRDLG
HHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QRVLRQLIEPDKIFLEPETPVILITREASTTLLAEFPRQKLAGIVTELGGANSHAAILAR
HHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMGVPAIIGVEGILSANIEKSQIIINANRGQVIIEPSALVLSEYRSLIAAQEEQQRQYAQ
HHCCCEEECCHHHHHCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
ELSLKAVTLDNKRIYLYINSGLLSSVDSETGADSDGIGLYRTEILFMLKQCFPGEIEQLE
HCCEEEEEECCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
IYRKVLKAAGNKPVVMRTLDVGGDKPLDYFPIIEDNPFLGWRGIRLSLDHPELFLVQLRA
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MLQAAIDTEQLHILLPMVSNLEEIDLSLIYLEQACQELTSELGKSITMPKVGIMLEVPVL
HHHHHCCHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHH
LYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPSVSPLFDSYHPGVLRALKFAVERCGEH
HHHHHHHHHHHCEEECCCHHHHHHHHHEECCCCCCCCCHHCCCHHHHHHHHHHHHHCCCC
QLPISVCGELAGEPIGAMLLVAMGYDQLSMNQASLARINYLLRRVSQTELNQLLGEALKL
CCCHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NSGEEVRLLVAQYCEQKGLASVLS
CCCCHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]