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The map label for this gene is ykoW [H]

Identifier: 167622986

GI number: 167622986

Start: 1284758

End: 1286053

Strand: Direct

Name: ykoW [H]

Synonym: Shal_1052

Alternate gene names: 167622986

Gene position: 1284758-1286053 (Clockwise)

Preceding gene: 167622979

Following gene: 167622987

Centisome position: 24.58

GC content: 40.2

Gene sequence:

>1296_bases
ATGAGCATACGCTTCAAACTCGGACTTCTTTTGTCCTTACTGTTTTTTGCTGCAATTGGTAATACTGTATTCACATTTAT
CCTTGAGGAGTATGGTGAGGAGAAGCTTGAATGGGTGATCCACACACATGAGGTGTTAACTGAATCGGAACGTCTAATTG
GCGCAGTTATCGATGCTGAAACGGGTCAGCGAGGCTACCTTTTAACCCAGGATTCGGCATATCTTGAGCCGTACCATATT
GGCGTGTCTTCGGTGGATTCACACCTTCAAGAGCTCTTCCGATTAACTATAGATAATTTGTCACAACAGAAGCGCTTAGA
AAATATAAGTGGGTTATTGACGAAAAAGTTAGCCGAGCTAAATTTAACGATTAACTTGACCCAAGAAAATACTCCCTCCT
CCATATCTGAAGCCTCTAACATTGTTAAAAATAACAGTGGTAAGGACTATATGGATGCAATAAGGGATGAGCTCACTTCG
TTTAATAATGAAGAGCTGGTGCTGCTAGAACAAAGGAAAGGCGCGTTTAACGAGAGTCGGGCATATATCACCACATTGAT
TGGAGCTGAGATCCTGTTTTTTATTTTTTTGTCGATTATTACAGCAATGTTTGTAAAAAATAAATTCTATCAACCATTAG
GCATGATGATTGAAGCTACGGCTAAAATGGAGCGGGGCGAAAGGCAAGAAGTTTCAGATATCCTGCCAAAAGATGAGATG
GGCTATTTACTCTCGCGTTTCTATCAAATGAGTGAAACCGTATACGCTAAAGCACAGGTGTTAGATCACGAAGCTAACCA
TGATTATCTCACCGGATTAAAGAATCGTACAGGCTTAGATACTGGAGTTGATTACTCTATAAAGGCACTGAGTTATGGCC
GAAAACTGGCAATATTTTTTATAGACCTAAATAAGTTTAAAGTGCTGAATGATACCTTAGGGCATGATGTTGGAGATGAA
ATTCTCAAGGAAGCAGCGTTACGTATCAAAGAGTCGGTGAGGGTGAATGATGCTGTGTATCGCTTCGGAGGAGATGAATT
TGTTGTTATTGTCAATGACATTAAGGAAGCGGATCATGCAAGAGTGATAGCCGAAAAGATGTTGGCTAAATTCGACTCGC
CATTTATTTGCAGGGGCAACGTAATCGATATATCGCTGAGTATCGGTATCTCTATTTCGCCAGAGGATTCATCACAAAGC
AGTGAGTTAATCAATCGGGCCGATATCGCTATGTACGCAGTAAAACGCGATGAGAGTATAAATTATAAATTCTTTGATAC
TTCAATGTTAAACTGA

Upstream 100 bases:

>100_bases
TAACGTTTTTGTTCCAGATAATTTAATTGCTAAGTGAATAAATATAATATGGCGATTAATTGATTCTAATAATTAAGCAG
TAAACTTTAAGGGGGCTCGA

Downstream 100 bases:

>100_bases
GCGAGTGATAGTGAGGATACCTCTTCACTATCACCATATGTAACAGAGACGAGTCACTGTAATATTAGTAGCCAAGTGAC
CTAGTACGAATCGGTATTAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSIRFKLGLLLSLLFFAAIGNTVFTFILEEYGEEKLEWVIHTHEVLTESERLIGAVIDAETGQRGYLLTQDSAYLEPYHI
GVSSVDSHLQELFRLTIDNLSQQKRLENISGLLTKKLAELNLTINLTQENTPSSISEASNIVKNNSGKDYMDAIRDELTS
FNNEELVLLEQRKGAFNESRAYITTLIGAEILFFIFLSIITAMFVKNKFYQPLGMMIEATAKMERGERQEVSDILPKDEM
GYLLSRFYQMSETVYAKAQVLDHEANHDYLTGLKNRTGLDTGVDYSIKALSYGRKLAIFFIDLNKFKVLNDTLGHDVGDE
ILKEAALRIKESVRVNDAVYRFGGDEFVVIVNDIKEADHARVIAEKMLAKFDSPFICRGNVIDISLSIGISISPEDSSQS
SELINRADIAMYAVKRDESINYKFFDTSMLN

Sequences:

>Translated_431_residues
MSIRFKLGLLLSLLFFAAIGNTVFTFILEEYGEEKLEWVIHTHEVLTESERLIGAVIDAETGQRGYLLTQDSAYLEPYHI
GVSSVDSHLQELFRLTIDNLSQQKRLENISGLLTKKLAELNLTINLTQENTPSSISEASNIVKNNSGKDYMDAIRDELTS
FNNEELVLLEQRKGAFNESRAYITTLIGAEILFFIFLSIITAMFVKNKFYQPLGMMIEATAKMERGERQEVSDILPKDEM
GYLLSRFYQMSETVYAKAQVLDHEANHDYLTGLKNRTGLDTGVDYSIKALSYGRKLAIFFIDLNKFKVLNDTLGHDVGDE
ILKEAALRIKESVRVNDAVYRFGGDEFVVIVNDIKEADHARVIAEKMLAKFDSPFICRGNVIDISLSIGISISPEDSSQS
SELINRADIAMYAVKRDESINYKFFDTSMLN
>Mature_430_residues
SIRFKLGLLLSLLFFAAIGNTVFTFILEEYGEEKLEWVIHTHEVLTESERLIGAVIDAETGQRGYLLTQDSAYLEPYHIG
VSSVDSHLQELFRLTIDNLSQQKRLENISGLLTKKLAELNLTINLTQENTPSSISEASNIVKNNSGKDYMDAIRDELTSF
NNEELVLLEQRKGAFNESRAYITTLIGAEILFFIFLSIITAMFVKNKFYQPLGMMIEATAKMERGERQEVSDILPKDEMG
YLLSRFYQMSETVYAKAQVLDHEANHDYLTGLKNRTGLDTGVDYSIKALSYGRKLAIFFIDLNKFKVLNDTLGHDVGDEI
LKEAALRIKESVRVNDAVYRFGGDEFVVIVNDIKEADHARVIAEKMLAKFDSPFICRGNVIDISLSIGISISPEDSSQSS
ELINRADIAMYAVKRDESINYKFFDTSMLN

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=157, Percent_Identity=35.6687898089172, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=32.9341317365269, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=36.875, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=32.5581395348837, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=172, Percent_Identity=31.3953488372093, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=30.952380952381, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=28.1437125748503, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=28.6585365853659, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081977, Length=160, Percent_Identity=31.25, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081974, Length=87, Percent_Identity=40.2298850574713, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 48754; Mature: 48623

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRFKLGLLLSLLFFAAIGNTVFTFILEEYGEEKLEWVIHTHEVLTESERLIGAVIDAE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECC
TGQRGYLLTQDSAYLEPYHIGVSSVDSHLQELFRLTIDNLSQQKRLENISGLLTKKLAEL
CCCCCEEEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
NLTINLTQENTPSSISEASNIVKNNSGKDYMDAIRDELTSFNNEELVLLEQRKGAFNESR
EEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCH
AYITTLIGAEILFFIFLSIITAMFVKNKFYQPLGMMIEATAKMERGERQEVSDILPKDEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHH
GYLLSRFYQMSETVYAKAQVLDHEANHDYLTGLKNRTGLDTGVDYSIKALSYGRKLAIFF
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCEEEHCCCCCEEEEEE
IDLNKFKVLNDTLGHDVGDEILKEAALRIKESVRVNDAVYRFGGDEFVVIVNDIKEADHA
EECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHEEECCCEEEEEEECCCCCHHH
RVIAEKMLAKFDSPFICRGNVIDISLSIGISISPEDSSQSSELINRADIAMYAVKRDESI
HHHHHHHHHHCCCCEEEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHHEEEEEECCCCC
NYKFFDTSMLN
CEEEECCCCCC
>Mature Secondary Structure 
SIRFKLGLLLSLLFFAAIGNTVFTFILEEYGEEKLEWVIHTHEVLTESERLIGAVIDAE
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECC
TGQRGYLLTQDSAYLEPYHIGVSSVDSHLQELFRLTIDNLSQQKRLENISGLLTKKLAEL
CCCCCEEEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
NLTINLTQENTPSSISEASNIVKNNSGKDYMDAIRDELTSFNNEELVLLEQRKGAFNESR
EEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCH
AYITTLIGAEILFFIFLSIITAMFVKNKFYQPLGMMIEATAKMERGERQEVSDILPKDEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHH
GYLLSRFYQMSETVYAKAQVLDHEANHDYLTGLKNRTGLDTGVDYSIKALSYGRKLAIFF
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCEEEHCCCCCEEEEEE
IDLNKFKVLNDTLGHDVGDEILKEAALRIKESVRVNDAVYRFGGDEFVVIVNDIKEADHA
EECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHEEECCCEEEEEEECCCCCHHH
RVIAEKMLAKFDSPFICRGNVIDISLSIGISISPEDSSQSSELINRADIAMYAVKRDESI
HHHHHHHHHHCCCCEEEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHHEEEEEECCCCC
NYKFFDTSMLN
CEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]