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The map label for this gene is potF [H]

Identifier: 167622935

GI number: 167622935

Start: 1224720

End: 1225820

Strand: Direct

Name: potF [H]

Synonym: Shal_0996

Alternate gene names: 167622935

Gene position: 1224720-1225820 (Clockwise)

Preceding gene: 167622934

Following gene: 167622936

Centisome position: 23.43

GC content: 44.05

Gene sequence:

>1101_bases
ATGAAACTTTTAACAAAAGCGACCACACTTGCCCTCGTCACCGCAAGTGTAATGGCTTCAACTACCGTATACGCTGAAGA
AGTGGTTCGAGTTTACAACTGGTCAGATTATATTGCCGAAGATACCCTAGAAAACTTCCAAAAAGAGACCGGTATTCGTG
TTGTTTACGATGTGTTTGACAGTAATGAAGTGGTTGAGGCTAAGCTGCTTTCTGGTCGCTCTGGCTATGATATCGTCGTA
CCTTCTAATAGTTTCTTAGCCAAGCAGATTAAAGCGGGGGCTTTCCAGAAGTTAGACTCAAATCAACTGCCAAACCATAA
GAATCTAAGTCCTGAGTTAATGAATCAGTTAGAGTCCGCTGATCCGGGTAATCAGTACTCTGTGCCATATCTATGGGGAA
CCAACGGTATCGGTTATAACGTGGAAAAAGTCACAGCAGTACTCGGTGAAGATGCCCCTGTCGATTCGTTAGAACTTATC
TTTAATCCTAAGTATGCCGAGAAGGTATCTCAGTGTGGCTTGTCTTTGTTAGATAGCGCTGATGAGATGATCCCAATGGC
GCTTATCTACCTAGGGCTGGATCCAAATAGTACTAAAGCCGATGATTTTAAGAAGGCAGGTGAAGTGCTTGCTAAGGTAC
GTCCTTACATCACCTACTTCCACTCGTCGCGTTATATCACCGACTTAGCGAATGGTGATATCTGTGTGGCATTTGGTTAT
TCAGGCGATATTTTCCAGGCTGCCGATCGCGCCGATGAGGCCGAAAACGGTAATACGATTGAATACTCCATTCCAAAAGA
GGGCTCAAATCTTTGGTTTGATATGTTAGCGATTCCTGCGGATGCGGCTAACGTTAAAAATGCTCACACCTTTATCAATT
ACCTGCTTCGTCCAGAGGTGATTGCCCCTATTACCAACTATGTGGCTTATGCGAATCCTAATGTTCCAGCTTTGCCTCTA
GTGGATGAAGATGTGCGTACGAATACTTCTATTTATCCATCAAAGGAAGTGCTTGATCGATTGTATGTTGGCGATGTACG
CCCAATGAAATCACAACGTTCATTAACGCGCGTGTGGACCAAAGTGAAATCAGGCTACTAA

Upstream 100 bases:

>100_bases
TAGCATTGGTGAAAAATAAGAACTAAAGCCGGTATATCGGCAACACAATGAATGGACAGCAGAGGTGGATTTACTGCTGC
TACGCTAAATAGGAGAAAGC

Downstream 100 bases:

>100_bases
GCAAATAAGGGTAAGGCATCGCCTTGCCCTTTTTGATCAACAGCTAATTTTGGCACAGTAAGTAATAAATGAATTGAAAC
AAAGGGCATTGATTATCGCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKLLTKATTLALVTASVMASTTVYAEEVVRVYNWSDYIAEDTLENFQKETGIRVVYDVFDSNEVVEAKLLSGRSGYDIVV
PSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMNQLESADPGNQYSVPYLWGTNGIGYNVEKVTAVLGEDAPVDSLELI
FNPKYAEKVSQCGLSLLDSADEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEVIAPITNYVAYANPNVPALPL
VDEDVRTNTSIYPSKEVLDRLYVGDVRPMKSQRSLTRVWTKVKSGY

Sequences:

>Translated_366_residues
MKLLTKATTLALVTASVMASTTVYAEEVVRVYNWSDYIAEDTLENFQKETGIRVVYDVFDSNEVVEAKLLSGRSGYDIVV
PSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMNQLESADPGNQYSVPYLWGTNGIGYNVEKVTAVLGEDAPVDSLELI
FNPKYAEKVSQCGLSLLDSADEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEVIAPITNYVAYANPNVPALPL
VDEDVRTNTSIYPSKEVLDRLYVGDVRPMKSQRSLTRVWTKVKSGY
>Mature_366_residues
MKLLTKATTLALVTASVMASTTVYAEEVVRVYNWSDYIAEDTLENFQKETGIRVVYDVFDSNEVVEAKLLSGRSGYDIVV
PSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMNQLESADPGNQYSVPYLWGTNGIGYNVEKVTAVLGEDAPVDSLELI
FNPKYAEKVSQCGLSLLDSADEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEVIAPITNYVAYANPNVPALPL
VDEDVRTNTSIYPSKEVLDRLYVGDVRPMKSQRSLTRVWTKVKSGY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=369, Percent_Identity=55.0135501355014, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=33.9233038348083, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787711, Length=322, Percent_Identity=24.223602484472, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40508; Mature: 40508

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLTKATTLALVTASVMASTTVYAEEVVRVYNWSDYIAEDTLENFQKETGIRVVYDVFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVVEAKLLSGRSGYDIVVPSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMNQLESA
CCCEEEHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
DPGNQYSVPYLWGTNGIGYNVEKVTAVLGEDAPVDSLELIFNPKYAEKVSQCGLSLLDSA
CCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHHCH
DEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
HHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCEEEEECC
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEV
CCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHH
IAPITNYVAYANPNVPALPLVDEDVRTNTSIYPSKEVLDRLYVGDVRPMKSQRSLTRVWT
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KVKSGY
HHHCCC
>Mature Secondary Structure
MKLLTKATTLALVTASVMASTTVYAEEVVRVYNWSDYIAEDTLENFQKETGIRVVYDVFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVVEAKLLSGRSGYDIVVPSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMNQLESA
CCCEEEHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
DPGNQYSVPYLWGTNGIGYNVEKVTAVLGEDAPVDSLELIFNPKYAEKVSQCGLSLLDSA
CCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHHCH
DEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
HHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCEEEEECC
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEV
CCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHH
IAPITNYVAYANPNVPALPLVDEDVRTNTSIYPSKEVLDRLYVGDVRPMKSQRSLTRVWT
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KVKSGY
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]