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The map label for this gene is puuA [H]

Identifier: 167622934

GI number: 167622934

Start: 1223093

End: 1224442

Strand: Direct

Name: puuA [H]

Synonym: Shal_0995

Alternate gene names: 167622934

Gene position: 1223093-1224442 (Clockwise)

Preceding gene: 167622933

Following gene: 167622935

Centisome position: 23.4

GC content: 46.81

Gene sequence:

>1350_bases
ATGAAAAAGTTAGTTAGTTATTTAAAAGATCAAAAAATTACCGAAGTAGAGTGTGTCGTCTGTGATATGACGGGCATTGC
CCGTGGCAAGATTGCACCAGTGGGTAAGTTTATTGACGAAAAAGGCATGCGTTTGCCTGAGAGTGTGTTGCTGCAAACTG
TCACCGGCGATTATGTGGATGACGCATCTTACGATGCGCTACTCGATGAGGCCGATATCGACTTTGTTTGTGTACCCGAT
GAAAACGCGGTGTTTAAACTGCCATGGACCATCGAAGCGACCGCACAGGTGATCCATGATACCTACGACAAGATGGGCAA
TCCTATCGAGTTATCTCCACGTAACCTACTGAAGAAAGTGCTTAGACTTTATGAAGATAAAGGCTGGAAGCCTGTGGTTG
CACCAGAGATGGAATTCTACTTAACTCGCATTAATGAAGATCCCGATCAACCGCTTATTCCCCCTGTAGGGCGTTCGGGT
CGACAGGAGTCGGGTCGTCAGTCTTTCTCTATCGACGCGGCAAATGAATACGATCCACTATTTGAAGATATGTACGATTG
GTGTGAAATTCAGGGGCTGGATATCGATACTTTGATCCACGAAGAAGGTACGGCGCAGATGGAGATTAACTTCTCACATG
GCGATGCCTTATCTCTTGCAGATCAAGTCTTTGTGTTTAAACGTACCCTGCGTGAGGCCGCGCTTAAGCACAATGTTTGC
GCCACCTTTATGGCCAAACCGATTACTAACGAACCCGGCAGCGCGATGCACCTGCATCAGAGTATTGTTGATGTTAAAAC
AGGTAAAAACATTTTCTCGAATGAGGATGGTACTAAGAGCCCGCTGTTTTATAGCTACATAGGTGGGCTGCAAAAGTTTA
TTCCTGAATTGTTGCCATTGCTTGCGCCAAACGTTAACTCTTTCCGCCGCTTCCTACCGGGCGCTTCTGCACCTGTAAAC
TTGGAGTGGGGTGAAGAGAACCGTACCTGTGGTTTGCGTATCCCTGAGTCGTCACCGCAAAATCTGCGTATTGAAAACCG
TGTCGCTGGCGCCGATGCCAACAGTTATTTGTCAATTGCAGCCAGCTTACTCTGTGGTTATATGGGCATGGTTGAAGACA
TTAAGCCATCAACACCCGTCCAGGGCCGCGCCAATGAAACTCGCAGTGGAACGGCATTGCCACTGACATTAGAGGAAGCC
TTGGCGGTAATGACAGAAAGCTCAGCTTGTAGAGAATATTTAGGTGAAACCTTTACCAATGGCTATGTAGCGGTTAAACA
AGCCGATTTGGAAAACTACCGCCAGGTGATTAGTTCTTGGGAACGTAAGTTTTTACTGCTGACAGTATAA

Upstream 100 bases:

>100_bases
ATCCGGAATGGAAGGCACTCGATAGTCCTTTTTATACCGCGATTTTTAATGCTTTTAGTCAAGCATGTCAGCACCGTGCA
GCAAGCAGAGTGAAATAATC

Downstream 100 bases:

>100_bases
ATACATCACGCTCTCAGAATAAATAAAAGGCGATAGGGTTCGTTTTGGGGACGGATCTTAACCGCCTAAAAAATGAAAAT
ATGAAAAGGAGTTATTCATA

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKLVSYLKDQKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVDDASYDALLDEADIDFVCVPD
ENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKVLRLYEDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSG
RQESGRQSFSIDAANEYDPLFEDMYDWCEIQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
ATFMAKPITNEPGSAMHLHQSIVDVKTGKNIFSNEDGTKSPLFYSYIGGLQKFIPELLPLLAPNVNSFRRFLPGASAPVN
LEWGEENRTCGLRIPESSPQNLRIENRVAGADANSYLSIAASLLCGYMGMVEDIKPSTPVQGRANETRSGTALPLTLEEA
LAVMTESSACREYLGETFTNGYVAVKQADLENYRQVISSWERKFLLLTV

Sequences:

>Translated_449_residues
MKKLVSYLKDQKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVDDASYDALLDEADIDFVCVPD
ENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKVLRLYEDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSG
RQESGRQSFSIDAANEYDPLFEDMYDWCEIQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
ATFMAKPITNEPGSAMHLHQSIVDVKTGKNIFSNEDGTKSPLFYSYIGGLQKFIPELLPLLAPNVNSFRRFLPGASAPVN
LEWGEENRTCGLRIPESSPQNLRIENRVAGADANSYLSIAASLLCGYMGMVEDIKPSTPVQGRANETRSGTALPLTLEEA
LAVMTESSACREYLGETFTNGYVAVKQADLENYRQVISSWERKFLLLTV
>Mature_449_residues
MKKLVSYLKDQKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVDDASYDALLDEADIDFVCVPD
ENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKVLRLYEDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSG
RQESGRQSFSIDAANEYDPLFEDMYDWCEIQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
ATFMAKPITNEPGSAMHLHQSIVDVKTGKNIFSNEDGTKSPLFYSYIGGLQKFIPELLPLLAPNVNSFRRFLPGASAPVN
LEWGEENRTCGLRIPESSPQNLRIENRVAGADANSYLSIAASLLCGYMGMVEDIKPSTPVQGRANETRSGTALPLTLEEA
LAVMTESSACREYLGETFTNGYVAVKQADLENYRQVISSWERKFLLLTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=460, Percent_Identity=27.1739130434783, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI87081870, Length=434, Percent_Identity=34.331797235023, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1790301, Length=261, Percent_Identity=32.9501915708812, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50066; Mature: 50066

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVSYLKDQKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVD
CHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
DASYDALLDEADIDFVCVPDENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKV
CCCHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LRLYEDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSGRQESGRQSFSIDAANEYDPL
HHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
FEDMYDWCEIQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHCCCCCHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPITNEPGSAMHLHQSIVDVKTGKNIFSNEDGTKSPLFYSYIGGLQKFIPELLPL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAPNVNSFRRFLPGASAPVNLEWGEENRTCGLRIPESSPQNLRIENRVAGADANSYLSIA
HCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECEECCCCCHHHHHHH
ASLLCGYMGMVEDIKPSTPVQGRANETRSGTALPLTLEEALAVMTESSACREYLGETFTN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCC
GYVAVKQADLENYRQVISSWERKFLLLTV
CEEEEEHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKKLVSYLKDQKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVD
CHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
DASYDALLDEADIDFVCVPDENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKV
CCCHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LRLYEDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSGRQESGRQSFSIDAANEYDPL
HHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
FEDMYDWCEIQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHCCCCCHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPITNEPGSAMHLHQSIVDVKTGKNIFSNEDGTKSPLFYSYIGGLQKFIPELLPL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAPNVNSFRRFLPGASAPVNLEWGEENRTCGLRIPESSPQNLRIENRVAGADANSYLSIA
HCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECEECCCCCHHHHHHH
ASLLCGYMGMVEDIKPSTPVQGRANETRSGTALPLTLEEALAVMTESSACREYLGETFTN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCC
GYVAVKQADLENYRQVISSWERKFLLLTV
CEEEEEHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]