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The map label for this gene is rstB [H]

Identifier: 167622870

GI number: 167622870

Start: 1149577

End: 1150845

Strand: Reverse

Name: rstB [H]

Synonym: Shal_0930

Alternate gene names: 167622870

Gene position: 1150845-1149577 (Counterclockwise)

Preceding gene: 167622871

Following gene: 167622868

Centisome position: 22.02

GC content: 46.26

Gene sequence:

>1269_bases
ATGAAACGTCTTTTTATTAGCCTTTATCTTCTGCTCAGCCTCGGTTTTCTCGGTATAGGCTGGACACTCGATAATTTATG
GCAAAACAACGTAGACGAAAAAGGTGCACTCGATGCACCTTTAGTCGCTCTGGCGCAAGTATTAGCAACTCTGCCACCAG
CAGAGCGGCAAACGATGCTAGACGCTATCGAAGCCAACCCTGATTTTCCCCTGACTTTACTCGACTCTGACCAAGTGGCA
TTTTCTAGCGAGCAACTTTTAGGCCCAGATAAAGTGATTGCGACCCCTTATGACATCAATCACGAGTTTCATTTTGTGGC
TGTGGGTGAACAAGTCTTAATGGCCGGACCTATCGCAATCGACCCTGTAGCCAGCTTGCGAAATATCTACAATATTTTCT
TCTATCTATTATTGGCTTTTATCGCACTCATTTGGGTCTGGCCACTGTCGAAAGATCTAAAAACCTTGCGCAAGGCCACC
AAAGAGTTTGGCCAAGCCAACTGGAACACACGGATCCAGTTAACCAAGCGCTCTCAAGTATTACCACTAGCAAATACCTT
TAACGATATGGCCGCTCATATTAGTGCGCTTATCGAAAACCAGAAACATCTCTCTAACGCGGTTTCTCATGAGATCCGCA
CGCCATTAGCTCGCCTTAAATTTGCCTTAGCCTTGCTGCCTCAATACTGCCTACCAGAGTCCGATGTCGCTAAACGTCAA
CGCTTCTTAGATGAGATGCAGCTTGATATCAAAGAGATGGAAAATCTTCTGCAAGAGCTGCTTACCTATGCCAGTCTTGA
GACCCAGCGAGAGTCGCTCAACTTTGAACGTTGCGATTTAAACGGCCTAGTGGCGCAGACAATCCGGCGATTACAGAGCC
ACCATCCGACGCCAATCATTCTCGATGTCCCTGAAGAGACGATATATTTGATTGCAGAACCATCATTAGTTGAGCGTGCA
CTGCAAAACCTCGTCACTAACGCGCAGCGATTTTCAACTGACGATATTATCGTTAAGATCTCTCAAGACAGTGATGGTGT
GCGTCTATCCGTTACCGATGATGGCGAGGGGATTTTAGAGGAAGATCAGAGTAAGATCTTTGAGCCTTTCTACCGTAGCG
CAAGTAGTAAAAACGGCAATAAAGGACACGGTTTAGGCTTAGCAATAATCAAGCGGATTATGGACAGGCATCACGGTGAA
GTGAGCCTACAAAGCCGTCCAGGCTTTACCCAATTTACCTTATACTGGCCTGCGGCTAATCAAGTCTAA

Upstream 100 bases:

>100_bases
CGATAATATTGAAACCCCCTTTAGGATTAAGACTGTCTGGGGTCAAGGTTATCTGTTTGCCCCTGAAGCATGGAATAATT
AATTCTGCTAACCGGTTAAG

Downstream 100 bases:

>100_bases
AATTGTCGATATCATCGCCAGGCTCAATTGCTGGCGCATTGTCAAAATCAAGACTGGCGTTCACTTTTGCTCTTTCGTCA
AGTATTTGGGCTTTTTGGGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLATLPPAERQTMLDAIEANPDFPLTLLDSDQVA
FSSEQLLGPDKVIATPYDINHEFHFVAVGEQVLMAGPIAIDPVASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKAT
KEFGQANWNTRIQLTKRSQVLPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCLPESDVAKRQ
RFLDEMQLDIKEMENLLQELLTYASLETQRESLNFERCDLNGLVAQTIRRLQSHHPTPIILDVPEETIYLIAEPSLVERA
LQNLVTNAQRFSTDDIIVKISQDSDGVRLSVTDDGEGILEEDQSKIFEPFYRSASSKNGNKGHGLGLAIIKRIMDRHHGE
VSLQSRPGFTQFTLYWPAANQV

Sequences:

>Translated_422_residues
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLATLPPAERQTMLDAIEANPDFPLTLLDSDQVA
FSSEQLLGPDKVIATPYDINHEFHFVAVGEQVLMAGPIAIDPVASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKAT
KEFGQANWNTRIQLTKRSQVLPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCLPESDVAKRQ
RFLDEMQLDIKEMENLLQELLTYASLETQRESLNFERCDLNGLVAQTIRRLQSHHPTPIILDVPEETIYLIAEPSLVERA
LQNLVTNAQRFSTDDIIVKISQDSDGVRLSVTDDGEGILEEDQSKIFEPFYRSASSKNGNKGHGLGLAIIKRIMDRHHGE
VSLQSRPGFTQFTLYWPAANQV
>Mature_422_residues
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLATLPPAERQTMLDAIEANPDFPLTLLDSDQVA
FSSEQLLGPDKVIATPYDINHEFHFVAVGEQVLMAGPIAIDPVASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKAT
KEFGQANWNTRIQLTKRSQVLPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCLPESDVAKRQ
RFLDEMQLDIKEMENLLQELLTYASLETQRESLNFERCDLNGLVAQTIRRLQSHHPTPIILDVPEETIYLIAEPSLVERA
LQNLVTNAQRFSTDDIIVKISQDSDGVRLSVTDDGEGILEEDQSKIFEPFYRSASSKNGNKGHGLGLAIIKRIMDRHHGE
VSLQSRPGFTQFTLYWPAANQV

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=438, Percent_Identity=28.9954337899543, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789808, Length=284, Percent_Identity=28.169014084507, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790861, Length=206, Percent_Identity=34.4660194174757, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790551, Length=297, Percent_Identity=29.2929292929293, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=25.0996015936255, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=28.7037037037037, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786783, Length=299, Percent_Identity=25.4180602006689, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=29.8642533936652, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786912, Length=214, Percent_Identity=28.9719626168224, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=26.890756302521, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=29.2792792792793, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=23.1046931407942, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=27.6018099547511, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789403, Length=223, Percent_Identity=25.5605381165919, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789149, Length=349, Percent_Identity=23.4957020057307, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=276, Percent_Identity=23.1884057971014, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790300, Length=234, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47552; Mature: 47552

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLATLPPAERQTML
CHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
DAIEANPDFPLTLLDSDQVAFSSEQLLGPDKVIATPYDINHEFHFVAVGEQVLMAGPIAI
HHHHCCCCCCEEEECCCCCCCCCHHCCCCCCEEECCEECCCCEEEEEECCHHHEECCCCC
DPVASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKATKEFGQANWNTRIQLTKRSQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
LPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCLPESDVAKRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RFLDEMQLDIKEMENLLQELLTYASLETQRESLNFERCDLNGLVAQTIRRLQSHHPTPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEE
LDVPEETIYLIAEPSLVERALQNLVTNAQRFSTDDIIVKISQDSDGVRLSVTDDGEGILE
EECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCHHH
EDQSKIFEPFYRSASSKNGNKGHGLGLAIIKRIMDRHHGEVSLQSRPGFTQFTLYWPAAN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCC
QV
CC
>Mature Secondary Structure
MKRLFISLYLLLSLGFLGIGWTLDNLWQNNVDEKGALDAPLVALAQVLATLPPAERQTML
CHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
DAIEANPDFPLTLLDSDQVAFSSEQLLGPDKVIATPYDINHEFHFVAVGEQVLMAGPIAI
HHHHCCCCCCEEEECCCCCCCCCHHCCCCCCEEECCEECCCCEEEEEECCHHHEECCCCC
DPVASLRNIYNIFFYLLLAFIALIWVWPLSKDLKTLRKATKEFGQANWNTRIQLTKRSQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
LPLANTFNDMAAHISALIENQKHLSNAVSHEIRTPLARLKFALALLPQYCLPESDVAKRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RFLDEMQLDIKEMENLLQELLTYASLETQRESLNFERCDLNGLVAQTIRRLQSHHPTPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEE
LDVPEETIYLIAEPSLVERALQNLVTNAQRFSTDDIIVKISQDSDGVRLSVTDDGEGILE
EECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCHHH
EDQSKIFEPFYRSASSKNGNKGHGLGLAIIKRIMDRHHGEVSLQSRPGFTQFTLYWPAAN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCC
QV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]