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The map label for this gene is ispB [H]

Identifier: 167622844

GI number: 167622844

Start: 1124758

End: 1125729

Strand: Reverse

Name: ispB [H]

Synonym: Shal_0904

Alternate gene names: 167622844

Gene position: 1125729-1124758 (Counterclockwise)

Preceding gene: 167622852

Following gene: 167622842

Centisome position: 21.54

GC content: 44.65

Gene sequence:

>972_bases
ATGGATTTAAACGCCATTCGTCAATTGACCGATAGCGATATGCAAGCTGTCAATCAATTGATCTATAAACAACTAGAGTC
AGATGTTGCCCTAATTAATCAACTTGGGTTCTACATTATTAACGGTGGCGGTAAACGCTTGCGACCTCTGCTATCTATTC
TAGCAGCACGCGCCATTGACTATAAAGGTGATTCTCACCTTAAATTAGCAGCAATTATCGAATTCATTCACACCGCATCA
CTTTTACATGATGATGTGGTTGATGAGTCTATGTTGCGCCGCGGCAGAAAAACCGCTAATGCCCTGTTCGGCAATAGTGC
AAGTGTATTGGTCGGTGACTTCCTATATACCCGCTCATTCCAGATGATGACTGAGCTCAAGTGTCTAGATGTATTAGAAA
TTCTTGCAGATGCAACCAACGTACTTGCTGAAGGTGAAGTTTTACAATTAATGAATTGTAATGACCCAGACACCACAGAA
GACAGCTATATGCGCGTGATCTACTGTAAGACTGCCAAGCTATTCGAGGCAGCGACTCGTCTTGCAGGCTTGTTAGCTGA
CAGCCCTATTGAAGTGCAAACCGCACTGGCTGAATACGGTAAGTATTTAGGTACCGCATTCCAGTTAACTGATGACCTTC
TCGATTACACTGCCGATACAGAAGAGCTAGGTAAGAACATCGGTGACGACTTAGCTGAAGGTAAGCCAACGCTGCCGCTA
ATTTTTGCGATGGCAAACGGTAACGACACTGAAAAGGCGCTTATCCGTGAGGCTATCGAGAAAGGTGACGGTACTGAGCA
TATCGAAGTTATCCTCGCCGCACTTAATAACTGTGGTGCACTCAAGTACACCGAGCAGCGCGCTATCGAAGAGTCAGAAA
AGGCCATTGCAGCACTGGCTATCATTCCTGAGTCTGATTATAAGACCGCATTAATCTCTTTAGCAAAACTAGCCACTCAA
CGTAGTAACTAA

Upstream 100 bases:

>100_bases
TCTTGTACCGCAAATTTAGGTAGAATCCATTCATTATAACATTTAAGCCTTAAATGAGCTGCAATGTTACTCCGCAGCCA
ACAAGAACTAGAGCCTACTT

Downstream 100 bases:

>100_bases
TCAGCTATTGCCACCTGTAAGCTAAGAGCAGTGACGAGATAAATGTTGAAAATAAAAAAGGAGCCTAAGGCTCCTTTTTT
ATTTTGACCCACAAAGGCCC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAIDYKGDSHLKLAAIIEFIHTAS
LLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSFQMMTELKCLDVLEILADATNVLAEGEVLQLMNCNDPDTTE
DSYMRVIYCKTAKLFEAATRLAGLLADSPIEVQTALAEYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
IFAMANGNDTEKALIREAIEKGDGTEHIEVILAALNNCGALKYTEQRAIEESEKAIAALAIIPESDYKTALISLAKLATQ
RSN

Sequences:

>Translated_323_residues
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAIDYKGDSHLKLAAIIEFIHTAS
LLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSFQMMTELKCLDVLEILADATNVLAEGEVLQLMNCNDPDTTE
DSYMRVIYCKTAKLFEAATRLAGLLADSPIEVQTALAEYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
IFAMANGNDTEKALIREAIEKGDGTEHIEVILAALNNCGALKYTEQRAIEESEKAIAALAIIPESDYKTALISLAKLATQ
RSN
>Mature_323_residues
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAIDYKGDSHLKLAAIIEFIHTAS
LLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSFQMMTELKCLDVLEILADATNVLAEGEVLQLMNCNDPDTTE
DSYMRVIYCKTAKLFEAATRLAGLLADSPIEVQTALAEYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
IFAMANGNDTEKALIREAIEKGDGTEHIEVILAALNNCGALKYTEQRAIEESEKAIAALAIIPESDYKTALISLAKLATQ
RSN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=321, Percent_Identity=29.9065420560748, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4758430, Length=265, Percent_Identity=25.2830188679245, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI83700220, Length=265, Percent_Identity=25.2830188679245, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=61.993769470405, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=32.3144104803493, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=322, Percent_Identity=31.9875776397516, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=289, Percent_Identity=32.8719723183391, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=26.8722466960352, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=30.8724832214765, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI281365769, Length=289, Percent_Identity=23.8754325259516, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24660002, Length=289, Percent_Identity=23.8754325259516, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHC
YKGDSHLKLAAIIEFIHTASLLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
QMMTELKCLDVLEILADATNVLAEGEVLQLMNCNDPDTTEDSYMRVIYCKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHH
LAGLLADSPIEVQTALAEYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCEE
IFAMANGNDTEKALIREAIEKGDGTEHIEVILAALNNCGALKYTEQRAIEESEKAIAALA
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEE
IIPESDYKTALISLAKLATQRSN
EECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHC
YKGDSHLKLAAIIEFIHTASLLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
QMMTELKCLDVLEILADATNVLAEGEVLQLMNCNDPDTTEDSYMRVIYCKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHH
LAGLLADSPIEVQTALAEYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCEE
IFAMANGNDTEKALIREAIEKGDGTEHIEVILAALNNCGALKYTEQRAIEESEKAIAALA
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEE
IIPESDYKTALISLAKLATQRSN
EECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]