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The map label for this gene is rluE [H]

Identifier: 167622752

GI number: 167622752

Start: 1015500

End: 1016111

Strand: Reverse

Name: rluE [H]

Synonym: Shal_0812

Alternate gene names: 167622752

Gene position: 1016111-1015500 (Counterclockwise)

Preceding gene: 167622758

Following gene: 167622748

Centisome position: 19.44

GC content: 45.75

Gene sequence:

>612_bases
ATGTCTGTTGTCGATAAATCTAGCGAACCTAAGCATCAACACTTTTTGATCTACAAGCCCTACGGTTTTCTAAGTCAATT
TGTACCTGAAACACGTAAGAGTAAACCGTTGCTCGGTCAGCTATATGAGTTCCCAGAGAAAACAATGGCAATTGGTCGAC
TCGACCATGACTCAGAAGGCCTGTTACTACTGACCACCGATGGGATGCAGAGCCACCTTATTCGTAGTAAAACCATTGAA
AAGGAATACTATGTGCAGGTTGATGGCGAAGTGAGTCAAGCACAACTCGCGAAGCTACGACAAGGAGTAGAAATTGGCAT
TAGCGGGGGTAAATATCTGACTTTACCCTGTAAGGTTGAACTATTGATCCACGAACCAAACCTTCCAGCAAGAGGAAAAA
AGATCCGCGATCCTCGCCACGGCCCAACCAATTGGCTTTCCGTTACTATAGATGAAGGTAAGAACCGTCAAGTAAGAAAG
ATGACCGCCGCTGTGGGCTTGCCGACGTTAAGATTGGTTAGAGTCAGGATTGGTGCTATTCATCTTAAGCAGATGCAACC
TGGCGAGGTTATCCCTGTGACCGGTTTTGCTATCGATACAGAGCATCGATAG

Upstream 100 bases:

>100_bases
CATTAGCTATTGTTTAAACCGTTTATCCGCTTTCTCGCTACTCAAGCGAAGCGCTTTAATTAACGGTATAATCAATGTCC
CCTTGTCCAGAGTCTCTTGT

Downstream 100 bases:

>100_bases
CAACGGCTTTAGGTATTACGACTTAACAACCTATAAGGATGGCGATTAAACTCTGTCGTCAGCAATTAAAAACGCTGTCA
GCTCTTCGACTAATTTAGGC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSVVDKSSEPKHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYEFPEKTMAIGRLDHDSEGLLLLTTDGMQSHLIRSKTIE
KEYYVQVDGEVSQAQLAKLRQGVEIGISGGKYLTLPCKVELLIHEPNLPARGKKIRDPRHGPTNWLSVTIDEGKNRQVRK
MTAAVGLPTLRLVRVRIGAIHLKQMQPGEVIPVTGFAIDTEHR

Sequences:

>Translated_203_residues
MSVVDKSSEPKHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYEFPEKTMAIGRLDHDSEGLLLLTTDGMQSHLIRSKTIE
KEYYVQVDGEVSQAQLAKLRQGVEIGISGGKYLTLPCKVELLIHEPNLPARGKKIRDPRHGPTNWLSVTIDEGKNRQVRK
MTAAVGLPTLRLVRVRIGAIHLKQMQPGEVIPVTGFAIDTEHR
>Mature_202_residues
SVVDKSSEPKHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYEFPEKTMAIGRLDHDSEGLLLLTTDGMQSHLIRSKTIEK
EYYVQVDGEVSQAQLAKLRQGVEIGISGGKYLTLPCKVELLIHEPNLPARGKKIRDPRHGPTNWLSVTIDEGKNRQVRKM
TAAVGLPTLRLVRVRIGAIHLKQMQPGEVIPVTGFAIDTEHR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=44.0217391304348, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787524, Length=189, Percent_Identity=34.3915343915344, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1788510, Length=187, Percent_Identity=32.0855614973262, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 22870; Mature: 22739

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVDKSSEPKHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYEFPEKTMAIGRLDHDSEG
CCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCHHHHHHHCCHHHEEEECCCCCCCC
LLLLTTDGMQSHLIRSKTIEKEYYVQVDGEVSQAQLAKLRQGVEIGISGGKYLTLPCKVE
EEEEEECCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCEEEEECCCEEEEEEEEEE
LLIHEPNLPARGKKIRDPRHGPTNWLSVTIDEGKNRQVRKMTAAVGLPTLRLVRVRIGAI
EEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHEEEEEECCEE
HLKQMQPGEVIPVTGFAIDTEHR
EEECCCCCCEEEEEEEEEECCCC
>Mature Secondary Structure 
SVVDKSSEPKHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYEFPEKTMAIGRLDHDSEG
CCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCHHHHHHHCCHHHEEEECCCCCCCC
LLLLTTDGMQSHLIRSKTIEKEYYVQVDGEVSQAQLAKLRQGVEIGISGGKYLTLPCKVE
EEEEEECCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCEEEEECCCEEEEEEEEEE
LLIHEPNLPARGKKIRDPRHGPTNWLSVTIDEGKNRQVRKMTAAVGLPTLRLVRVRIGAI
EEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHEEEEEECCEE
HLKQMQPGEVIPVTGFAIDTEHR
EEECCCCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA