Click here to switch to the map view.

The map label for this gene is mpl [H]

Identifier: 167622683

GI number: 167622683

Start: 924411

End: 925796

Strand: Direct

Name: mpl [H]

Synonym: Shal_0743

Alternate gene names: 167622683

Gene position: 924411-925796 (Clockwise)

Preceding gene: 167622679

Following gene: 167622684

Centisome position: 17.69

GC content: 48.2

Gene sequence:

>1386_bases
ATGCACGTACATATTTTAGGAATTTGTGGCACTTTCATGGGCGGCTTAGCGCTATTAGCTAGAGCAGAAGGGCACAAGGT
AACGGGCAGCGATGCCAATGTTTATCCTCCGATGAGCACTCAGCTTGAAGAGCAGGGCATTGAGCTTATTCAAGGCTTCG
ATCCGTCTCAGCTGGGAAGCTCTGAGGACGACGCACCCGACTTGGTCGTGATTGGTAATGCTATGAGCCGAGGTAACCCT
TGCGTCGAAGCGGTATTAAATCGCGGCCTTAAATATACCTCGGGTCCGCAATTTCTTGCCGAGCATATTCTGCCCCAGCG
TTGGGTGTTAGCGGTATCGGGTACCCATGGTAAAACATCGACCTCGAGCATGTTGGCGTGGATTTTAGAAGATTGCGGCT
ATGAGCCCGGCTTCTTGATTGGCGGCGTACCACAGAACTTTGGTGTATCGGCTCGTCTTGGTGGCTCACCCTTTTTTGTC
GTTGAAGCCGATGAGTACGATAGTGCATTTTTCGATAAACGCTCTAAATTTGTCCACTATCAGCCGCGCACCTTAGTGAT
TAATAACTTAGAGTTCGATCACGCGGATATCTTTGATGATTTAAAAGCCATTCAACGTCAGTTTAATCACGTAATACGTA
CCGTAGCGGGAGAAGGCAAAATCATATGGCCAGCCGATGCGGTCAGTGTGCAGCAAGTGATCGAAATGGGTTGCTGGAGC
GAGCAAGAGACTTATCATCTTGCACCAAGCTCACAGGGTTGGCATGCGGTGAACCTAACCCAAGATGGCCACAAATTTGA
AGTCTTCTTCGATGGTGAGTCGCAAGGTGTTTTAGACTGGCAGTTAATCGGTCAGCACAATATTGAAAACGCCGTTATGG
CGATAGCTGCAGCAAGGCATGTGGGCGTAAAACCCGATGCGGCGATTGCGGCATTAACGCAATTTGCACCGCCTAAGCGC
CGCATGGAACTGCTCGATACGGTTAATGGTATCGAAGTCTATGATGACTTTGCTCACCACCCAACGGCGATTACTACCTC
ACTGAGTGGCATGCGTGCTCGAGTAGGCGAAGCTAAGGTGACAGTGATTTTAGAGCCCCGCTCTAACACCATGAAAAGCG
GCGTGCATAAAGATACTTTAGCTAACTCGTTAGCGTTGGCCAGTAATGCTTATCTATATCAAGCGGGTAATATTGACTGG
GACATGCGTTCTGCTATGAGTTCAGCCTCTATTCCGGTTGAGGTCTTGTATGATATTGACGAGATTGTTGACAAGGTCGC
TGCTCAAGCCTGTAGCACAGATACTATTATTGTAATGAGTAATGGTGGTTTCGGTGGATTACATCAAAAGCTGCTTGCTA
AACTTCGCAGTCAAATGGGTAAATAA

Upstream 100 bases:

>100_bases
CGTCTGTTGTAATTGCGTCGATTTAAACGCGAGTTAACTGACAAAGCTTGTCACAATTGGGTAGAATGAGGCAGATTTTT
CATTCATCAGAGTGACAGGC

Downstream 100 bases:

>100_bases
TTAGTCATGAGTGTGACATATCGTAAAGCAGAAAAGTCAATCAGCTTGGCATGGACAGGTGCATCTGGCGCTCCCTACGG
ACTTAGGCTGCTAGAGTGTT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGSSEDDAPDLVVIGNAMSRGNP
CVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVSGTHGKTSTSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFV
VEADEYDSAFFDKRSKFVHYQPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVAGEGKIIWPADAVSVQQVIEMGCWS
EQETYHLAPSSQGWHAVNLTQDGHKFEVFFDGESQGVLDWQLIGQHNIENAVMAIAAARHVGVKPDAAIAALTQFAPPKR
RMELLDTVNGIEVYDDFAHHPTAITTSLSGMRARVGEAKVTVILEPRSNTMKSGVHKDTLANSLALASNAYLYQAGNIDW
DMRSAMSSASIPVEVLYDIDEIVDKVAAQACSTDTIIVMSNGGFGGLHQKLLAKLRSQMGK

Sequences:

>Translated_461_residues
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGSSEDDAPDLVVIGNAMSRGNP
CVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVSGTHGKTSTSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFV
VEADEYDSAFFDKRSKFVHYQPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVAGEGKIIWPADAVSVQQVIEMGCWS
EQETYHLAPSSQGWHAVNLTQDGHKFEVFFDGESQGVLDWQLIGQHNIENAVMAIAAARHVGVKPDAAIAALTQFAPPKR
RMELLDTVNGIEVYDDFAHHPTAITTSLSGMRARVGEAKVTVILEPRSNTMKSGVHKDTLANSLALASNAYLYQAGNIDW
DMRSAMSSASIPVEVLYDIDEIVDKVAAQACSTDTIIVMSNGGFGGLHQKLLAKLRSQMGK
>Mature_461_residues
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGSSEDDAPDLVVIGNAMSRGNP
CVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVSGTHGKTSTSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFV
VEADEYDSAFFDKRSKFVHYQPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVAGEGKIIWPADAVSVQQVIEMGCWS
EQETYHLAPSSQGWHAVNLTQDGHKFEVFFDGESQGVLDWQLIGQHNIENAVMAIAAARHVGVKPDAAIAALTQFAPPKR
RMELLDTVNGIEVYDDFAHHPTAITTSLSGMRARVGEAKVTVILEPRSNTMKSGVHKDTLANSLALASNAYLYQAGNIDW
DMRSAMSSASIPVEVLYDIDEIVDKVAAQACSTDTIIVMSNGGFGGLHQKLLAKLRSQMGK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=459, Percent_Identity=59.9128540305011, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1786279, Length=470, Percent_Identity=25.7446808510638, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50121; Mature: 50121

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGS
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCC
SEDDAPDLVVIGNAMSRGNPCVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVSGTHGKTS
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCH
TSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFVVEADEYDSAFFDKRSKFVHY
HHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHCCCCEEEE
QPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVAGEGKIIWPADAVSVQQVIEMGCWS
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHCCCC
EQETYHLAPSSQGWHAVNLTQDGHKFEVFFDGESQGVLDWQLIGQHNIENAVMAIAAARH
CCCEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHH
VGVKPDAAIAALTQFAPPKRRMELLDTVNGIEVYDDFAHHPTAITTSLSGMRARVGEAKV
CCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECHHHHHHHCCCEEE
TVILEPRSNTMKSGVHKDTLANSLALASNAYLYQAGNIDWDMRSAMSSASIPVEVLYDID
EEEECCCCCHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHH
EIVDKVAAQACSTDTIIVMSNGGFGGLHQKLLAKLRSQMGK
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGS
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCC
SEDDAPDLVVIGNAMSRGNPCVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVSGTHGKTS
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCH
TSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFVVEADEYDSAFFDKRSKFVHY
HHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHCCCCEEEE
QPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVAGEGKIIWPADAVSVQQVIEMGCWS
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHCCCC
EQETYHLAPSSQGWHAVNLTQDGHKFEVFFDGESQGVLDWQLIGQHNIENAVMAIAAARH
CCCEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHH
VGVKPDAAIAALTQFAPPKRRMELLDTVNGIEVYDDFAHHPTAITTSLSGMRARVGEAKV
CCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECHHHHHHHCCCEEE
TVILEPRSNTMKSGVHKDTLANSLALASNAYLYQAGNIDWDMRSAMSSASIPVEVLYDID
EEEECCCCCHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHH
EIVDKVAAQACSTDTIIVMSNGGFGGLHQKLLAKLRSQMGK
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]