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The map label for this gene is rluF [H]

Identifier: 167622673

GI number: 167622673

Start: 912015

End: 912749

Strand: Reverse

Name: rluF [H]

Synonym: Shal_0733

Alternate gene names: 167622673

Gene position: 912749-912015 (Counterclockwise)

Preceding gene: 167622674

Following gene: 167622670

Centisome position: 17.46

GC content: 50.61

Gene sequence:

>735_bases
ATGAGACTCGCAAAATACCTTGCCCTAACCGGCTGTTGCTCCCGTCGTGCGGCGACCCGCTTGATAAGAGACGGCCAAGT
TCGCATTAATGACCGACTCGCAAATCATGTCGATAGCGTCAGCCTCATCGACACCCCAGAAGGACAGCGCAGCGCCGAGC
AGATCTATGTTAACGATGAGCTCGTTTGCCCTATCGAAGCCAAGGCCTATTGGTTGTTTAATAAATCCGTCGGCACAGAT
AGCCGCTTGCTCGCTCATCTACCAAACAGTCTAATACACCTTTTGCCAGAGACACCGCGGCTCTACCCCATCGGCCGTCT
CGATAAAGACAGTCGTGGCTTACTGATCTTAACCAACGATGGTGAGCTGACCCATAAGCTAATGCACCCCGATTTTGGCC
ATGCTAAGACTTACCATGTGCAGCTCGACCGTGATTTTGATGATGCTTTTCTGGCAAGCATGGCCGCCGGGGTGAGTTAT
AAGGATGTCACCACCTTGCCGTGTATCATGCAGCGTTTAGCTGCTGATAAGTATCAGATTACGTTGACCCAGGGGCTTAA
TCGTCAAATACGCCGTATGTCTCAGGCTTTGGGGTTTAAGGTTATCGACCTTAAACGGGTATCGATTCAGAGTTTGCAGT
TAGGGGATTTGGCAGAAGGGTCGATGAGACCTTTGAGTTCTGCAGAGTTAGCAGATTTGTTGGCTTCTGTAGCTTGCCCA
GAAAGCAGCGCTTGA

Upstream 100 bases:

>100_bases
CTTTGGTGTCTTGCATTCGCTTGGAGCATACGTAATATTGCGATTAAAAATTACCTAGAACTGTCACAGCGCTACTTAGC
TGGAGAGTATTAAGCCCCTC

Downstream 100 bases:

>100_bases
GCTTGGAGTTAGACTTTGTCTAACAAGTTCTGTTGGAGGTAATCAAGCTGCGCTTGATTAGCTTACAATGGTTACACGCA
GTCTAACTGAAAGCTCAATA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRLAKYLALTGCCSRRAATRLIRDGQVRINDRLANHVDSVSLIDTPEGQRSAEQIYVNDELVCPIEAKAYWLFNKSVGTD
SRLLAHLPNSLIHLLPETPRLYPIGRLDKDSRGLLILTNDGELTHKLMHPDFGHAKTYHVQLDRDFDDAFLASMAAGVSY
KDVTTLPCIMQRLAADKYQITLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLAEGSMRPLSSAELADLLASVACP
ESSA

Sequences:

>Translated_244_residues
MRLAKYLALTGCCSRRAATRLIRDGQVRINDRLANHVDSVSLIDTPEGQRSAEQIYVNDELVCPIEAKAYWLFNKSVGTD
SRLLAHLPNSLIHLLPETPRLYPIGRLDKDSRGLLILTNDGELTHKLMHPDFGHAKTYHVQLDRDFDDAFLASMAAGVSY
KDVTTLPCIMQRLAADKYQITLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLAEGSMRPLSSAELADLLASVACP
ESSA
>Mature_244_residues
MRLAKYLALTGCCSRRAATRLIRDGQVRINDRLANHVDSVSLIDTPEGQRSAEQIYVNDELVCPIEAKAYWLFNKSVGTD
SRLLAHLPNSLIHLLPETPRLYPIGRLDKDSRGLLILTNDGELTHKLMHPDFGHAKTYHVQLDRDFDDAFLASMAAGVSY
KDVTTLPCIMQRLAADKYQITLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLAEGSMRPLSSAELADLLASVACP
ESSA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=36.864406779661, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788510, Length=243, Percent_Identity=29.6296296296296, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787524, Length=252, Percent_Identity=29.3650793650794, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081838, Length=145, Percent_Identity=33.7931034482759, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27102; Mature: 27102

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAKYLALTGCCSRRAATRLIRDGQVRINDRLANHVDSVSLIDTPEGQRSAEQIYVNDE
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCEEEEECCCCCCCCCEEEECCC
LVCPIEAKAYWLFNKSVGTDSRLLAHLPNSLIHLLPETPRLYPIGRLDKDSRGLLILTND
EEEEECCEEEEEEECCCCCCHHHHHHCCHHHHHHCCCCCCEEECCCCCCCCCCEEEEECC
GELTHKLMHPDFGHAKTYHVQLDRDFDDAFLASMAAGVSYKDVTTLPCIMQRLAADKYQI
CHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEE
TLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLAEGSMRPLSSAELADLLASVACP
EEHHHHHHHHHHHHHHHCEEEEEEEEHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
ESSA
CCCC
>Mature Secondary Structure
MRLAKYLALTGCCSRRAATRLIRDGQVRINDRLANHVDSVSLIDTPEGQRSAEQIYVNDE
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCEEEEECCCCCCCCCEEEECCC
LVCPIEAKAYWLFNKSVGTDSRLLAHLPNSLIHLLPETPRLYPIGRLDKDSRGLLILTND
EEEEECCEEEEEEECCCCCCHHHHHHCCHHHHHHCCCCCCEEECCCCCCCCCCEEEEECC
GELTHKLMHPDFGHAKTYHVQLDRDFDDAFLASMAAGVSYKDVTTLPCIMQRLAADKYQI
CHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEE
TLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLAEGSMRPLSSAELADLLASVACP
EEHHHHHHHHHHHHHHHCEEEEEEEEHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
ESSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]