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The map label for this gene is pyrI [H]

Identifier: 167622622

GI number: 167622622

Start: 840811

End: 841269

Strand: Reverse

Name: pyrI [H]

Synonym: Shal_0682

Alternate gene names: 167622622

Gene position: 841269-840811 (Counterclockwise)

Preceding gene: 167622623

Following gene: 167622621

Centisome position: 16.09

GC content: 37.91

Gene sequence:

>459_bases
ATGAAAAAACATCTCAAGGTAGAAGCTATTGAACAAGGTACTGTAATCGATCATATTTCATCCGGCCAAGGTATCAAAAT
ATTGAAATTTTTTGAACTCAGTAAAGGGCTACAACGCGTTACAATAGGCTTGAATTTACCGACTTATAGTGGTGGAAACA
AAGATTTGATCAAGATAGAAAATACAGTATTTGATCAAAAGGAGGCTAATCAGCTATCTCTATTCGCGCCACAGGCCACC
ATTAACGTGATCGAAAACTTCGAAGTTGTTAAAAAGTTTAAGCTGGCATTACCTGAACAAATATCAGGTGTATTAACCTG
CCCTAATAGTAACTGTATTTGCCATAACGAACCTGTTGATAGTCGCTTTAGCATCAGGCAAGAGCCCGTTGCAGTGAAGC
TAAAATGTCACTACTGTGAAAAATCATTTACAGCTACATCCTTTCAAGAGCTGCGCTAA

Upstream 100 bases:

>100_bases
GCACACCTTATGCATATTACTTCCAACAAGCAAAAAATGGTGTATATGCCCGTCAAGCTCTACTCACCTTAGTTTTAACA
AATGAATTTGGAGACCTGTG

Downstream 100 bases:

>100_bases
TAGCTAATCAATCAGCCTTTATCACTTATCAAATGAAGGCCGCGATTTCTATATCGTGGCCTTTTATTTGGGTCGACACC
CAAGGGCGAGTCTATAGTTT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKKHLKVEAIEQGTVIDHISSGQGIKILKFFELSKGLQRVTIGLNLPTYSGGNKDLIKIENTVFDQKEANQLSLFAPQAT
INVIENFEVVKKFKLALPEQISGVLTCPNSNCICHNEPVDSRFSIRQEPVAVKLKCHYCEKSFTATSFQELR

Sequences:

>Translated_152_residues
MKKHLKVEAIEQGTVIDHISSGQGIKILKFFELSKGLQRVTIGLNLPTYSGGNKDLIKIENTVFDQKEANQLSLFAPQAT
INVIENFEVVKKFKLALPEQISGVLTCPNSNCICHNEPVDSRFSIRQEPVAVKLKCHYCEKSFTATSFQELR
>Mature_152_residues
MKKHLKVEAIEQGTVIDHISSGQGIKILKFFELSKGLQRVTIGLNLPTYSGGNKDLIKIENTVFDQKEANQLSLFAPQAT
INVIENFEVVKKFKLALPEQISGVLTCPNSNCICHNEPVDSRFSIRQEPVAVKLKCHYCEKSFTATSFQELR

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=140, Percent_Identity=56.4285714285714, Blast_Score=170, Evalue=4e-44,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 17051; Mature: 17051

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHLKVEAIEQGTVIDHISSGQGIKILKFFELSKGLQRVTIGLNLPTYSGGNKDLIKIE
CCCCCEEEEECCCCEEEECCCCCCEEEEEEEHHHCCCEEEEEEEECCCCCCCCCCEEEEE
NTVFDQKEANQLSLFAPQATINVIENFEVVKKFKLALPEQISGVLTCPNSNCICHNEPVD
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCC
SRFSIRQEPVAVKLKCHYCEKSFTATSFQELR
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHCC
>Mature Secondary Structure
MKKHLKVEAIEQGTVIDHISSGQGIKILKFFELSKGLQRVTIGLNLPTYSGGNKDLIKIE
CCCCCEEEEECCCCEEEECCCCCCEEEEEEEHHHCCCEEEEEEEECCCCCCCCCCEEEEE
NTVFDQKEANQLSLFAPQATINVIENFEVVKKFKLALPEQISGVLTCPNSNCICHNEPVD
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCC
SRFSIRQEPVAVKLKCHYCEKSFTATSFQELR
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA