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The map label for this gene is metB [H]

Identifier: 167622562

GI number: 167622562

Start: 749947

End: 751107

Strand: Direct

Name: metB [H]

Synonym: Shal_0622

Alternate gene names: 167622562

Gene position: 749947-751107 (Clockwise)

Preceding gene: 167622559

Following gene: 167622563

Centisome position: 14.35

GC content: 48.41

Gene sequence:

>1161_bases
ATGACTGAGCGTAAACCAGCCACTGCGGCGGTACGTCAAGGAATTGAGTCCGATAGTCAGCATGGTGCCGTTGTTCCGCC
GATTTATCTATCTACTAACTACTCTTTTGATGGCCATAACAATCCAAGAGACTTCGACTATAGCCGCTCAGGCAATCCAA
CCCGATCTATTCTAGGCGATGCCATCGCCGAGCTGGAAAAAGGCGCAACTGGTGTTGTGACCTGTACCGGAATGGCTGCG
ATTACTTTAGCGACAAGCTTGCTAGGACCCGATGACTTACTCGTTGTTCCCCATGATTGTTATGGCGGCAGCTATCGTCT
ATTCACCAATTTAGCTAAAAAGGGCCAGTTTAAGTTATTAGTGGTGGATCAAACCGACTCACAAGCGCTCAATCAGGCGA
TCGCCAAGAAACCTAAGATGGTCTGGTTAGAAACTCCATCGAACCCGCTGCTGCGTGTGGTAGACATTGAGGCTATCAGC
TCAGCCAGTCATGAGGTTGGCGCATTGGTGGTTGTCGACAACACTTTTCTATCGCCAATTTTACAGCAGCCACTGCTTCT
TGGTGCAGATATTGTGATCCATTCAACCACTAAATATATCAATGGTCATAGTGATGTGGTGGGCGGCGCGGTTGTGGCAA
AAGATCCGGAGTTGGGTGAACTGCTGCACTGGTGGTCAAATACGCTAGGGCTAACGGGCTCAGCATTTGATAGTTATCTC
ACTTTGCGCGGAATTCGTACACTTGCAGTGAGAATTCGTGAACATCAGGCGAATGCACAGCGAATTCTTGATACCCTTGT
TAACCATCAGGCCGTGTCCAAGATATACTACCCGGGACTTAAAGAGCATCCGGGTCATGAAATTGCGACTAAACAGCAAA
AGGGCTATGGGGCCATGCTGAGTTTTGAGCTCAAGGGTAGCGAAGAACAGTTGGTCGCATTCTTAAAAAATCTTAACTTT
TTTTCAGTTGCAGAAAGTCTTGGTGGCGTAGAAAGCTTAGTCGCCGTACCTGCAACTATGACGCATAGGGCGATGGAGCC
AGAGGCACGCGCTGAAGCTGGGGTTAAGGATACGCTTATCCGTCTTTCTGTTGGTATCGAAGATGCTGATGATCTTGTTG
CCGATATTGAAGCTGCGCTTAATGCCGCAGCCGCTTGTTAA

Upstream 100 bases:

>100_bases
CGTCTAAACGGCTAAAATGGTTGACGAGGCGTGAGTGCTTAGGTACATTTAGACGTCCAGACATCTCTTGGCTGAAAATT
AACAGGAAAAGGAGTAGTAC

Downstream 100 bases:

>100_bases
TTAAAGGGGTATACGATGGCACGCTGTCACTTACATAAATTTGGTGGCTCAAGTCTTGCGGATGCTGACTGTTACCGTCG
CGTTGCTCATATTCTGCTGA

Product: O-succinylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTERKPATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHNNPRDFDYSRSGNPTRSILGDAIAELEKGATGVVTCTGMAA
ITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDSQALNQAIAKKPKMVWLETPSNPLLRVVDIEAIS
SASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIATKQQKGYGAMLSFELKGSEEQLVAFLKNLNF
FSVAESLGGVESLVAVPATMTHRAMEPEARAEAGVKDTLIRLSVGIEDADDLVADIEAALNAAAAC

Sequences:

>Translated_386_residues
MTERKPATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHNNPRDFDYSRSGNPTRSILGDAIAELEKGATGVVTCTGMAA
ITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDSQALNQAIAKKPKMVWLETPSNPLLRVVDIEAIS
SASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIATKQQKGYGAMLSFELKGSEEQLVAFLKNLNF
FSVAESLGGVESLVAVPATMTHRAMEPEARAEAGVKDTLIRLSVGIEDADDLVADIEAALNAAAAC
>Mature_385_residues
TERKPATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHNNPRDFDYSRSGNPTRSILGDAIAELEKGATGVVTCTGMAAI
TLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDSQALNQAIAKKPKMVWLETPSNPLLRVVDIEAISS
ASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYLT
LRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIATKQQKGYGAMLSFELKGSEEQLVAFLKNLNFF
SVAESLGGVESLVAVPATMTHRAMEPEARAEAGVKDTLIRLSVGIEDADDLVADIEAALNAAAAC

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=379, Percent_Identity=43.2717678100264, Blast_Score=285, Evalue=3e-77,
Organism=Homo sapiens, GI299473759, Length=379, Percent_Identity=41.1609498680739, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=38.2271468144044, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=60.8923884514436, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789383, Length=396, Percent_Identity=30.5555555555556, Blast_Score=160, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=347, Percent_Identity=45.5331412103746, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17538055, Length=364, Percent_Identity=44.5054945054945, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=32.8804347826087, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=42.1188630490956, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=35.2216748768473, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=30.8860759493671, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=31.042654028436, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=27.5541795665635, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6322970, Length=256, Percent_Identity=29.296875, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323557, Length=242, Percent_Identity=28.5123966942149, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322590, Length=277, Percent_Identity=27.4368231046931, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=41.7322834645669, Blast_Score=307, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41434; Mature: 41302

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKPATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHNNPRDFDYSRSGNPTRSILGD
CCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
AIAELEKGATGVVTCTGMAAITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLL
HHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
VVDQTDSQALNQAIAKKPKMVWLETPSNPLLRVVDIEAISSASHEVGALVVVDNTFLSPI
EEECCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCEEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
HHCHHHHCCEEEEECCHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIATKQQKGYGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
SFELKGSEEQLVAFLKNLNFFSVAESLGGVESLVAVPATMTHRAMEPEARAEAGVKDTLI
EEEECCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCHHHHHHCCCCHHHHHCCCHHEEE
RLSVGIEDADDLVADIEAALNAAAAC
EEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TERKPATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHNNPRDFDYSRSGNPTRSILGD
CCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
AIAELEKGATGVVTCTGMAAITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLL
HHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
VVDQTDSQALNQAIAKKPKMVWLETPSNPLLRVVDIEAISSASHEVGALVVVDNTFLSPI
EEECCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCEEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
HHCHHHHCCEEEEECCHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIATKQQKGYGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
SFELKGSEEQLVAFLKNLNFFSVAESLGGVESLVAVPATMTHRAMEPEARAEAGVKDTLI
EEEECCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCHHHHHHCCCCHHHHHCCCHHEEE
RLSVGIEDADDLVADIEAALNAAAAC
EEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]