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The map label for this gene is purR [H]

Identifier: 167622523

GI number: 167622523

Start: 699775

End: 700779

Strand: Direct

Name: purR [H]

Synonym: Shal_0583

Alternate gene names: 167622523

Gene position: 699775-700779 (Clockwise)

Preceding gene: 167622522

Following gene: 167622526

Centisome position: 13.39

GC content: 51.24

Gene sequence:

>1005_bases
ATGGCCACCATGAAAGATGTTGCCAAACTTGCCAGCGTATCCACATCTACGGTGAGTCATGTGATGAATGCTACCCGCTT
TGTGAGTGAGGATATTGCCAAGCGGGTGCAAGCGGCTGCGCAGCAACTGAACTATCACGGTCCATCGGCGGTGGCCCGCA
GCCTTAAACAGAACTGCACCAAAACCATCGGCATGTTGGTGACCACCTCGACCAACCCCTTCTATGCTGAGGTGGTTCGT
AGTGTTGAGCAGGCGTGTTACCAGCAGGGTTACAACCTTATTCTGTGTAATACCGAAGGTGATGCCCAGCGCTTAAAACG
TTCATTAACGACGTTAATTGAGCGCCGTGTCGACGGTTTAATCATGATGTGCGCCATGTTAGAAGGCGAGGATGCACTAA
TCTATCGCCGTCTAGACTCGCTACCTGTAGTGGTGATGGATTGGGGGCAGGTGGATTTTCCTTGTGACAAAATTCAGGGC
CACTCCCGCCAAGGTGGCATACTGGCGACTCAATATCTGATTGATAACGGGCATACTCAAATCGGCTGTATTACCGGGCC
ACGTAATCGTCAGCAGGCCGATCTGCGCTATCAGGGCTTTGTCGATGCGATGCAAGCGGCTAATCTACCGATTAACCCTG
ATTGGGTGGTTGAAGGGAACTTTGAAACCGAGGGCGGTAAGGCGGCGTTTGCTAAGCTACAAGCGGTGGGTAGCTTACCG
ACAGCACTTTTTGTGTGTAATGACATGATGGCGATTGGTGTATTACATGAAGCGCAGCGCACTGGGGTGCAGATCCCCGA
GCAGTTATCGGTTATCGGTTACGATAATATTCAATTAGCTAAATTTATGACCCCAGCGCTTACCAGTATTCATCAGTCGA
AAGCACGCTTAGGTGCGGCGGCGGTGCATGCCTTGATTGCCAGACTGCAAGATCCAAACCTGCCACCACAATTTATGGAT
ATTGAGCCTAGAGTGACGGTGCGCGCCAGCGTGAGTCAAAGGTAA

Upstream 100 bases:

>100_bases
GCTGCGCACTAAAATCGCACTAGATAGCAGTGAATACTGTTATTGAGAGAGTTATTAATACCGCTTTTAACATAGTGATT
CAGATAGGAAGTTAACCCCC

Downstream 100 bases:

>100_bases
AAAATAGAGTTTAGAAGAAAGAGTTTGAAGAAAGAATGTGGAAAATAGTTAAGTAGGATAAATCGCGAGTGAATCATAGC
TAATGACTCACTCGCCTATA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATMKDVAKLASVSTSTVSHVMNATRFVSEDIAKRVQAAAQQLNYHGPSAVARSLKQNCTKTIGMLVTTSTNPFYAEVVR
SVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGLIMMCAMLEGEDALIYRRLDSLPVVVMDWGQVDFPCDKIQG
HSRQGGILATQYLIDNGHTQIGCITGPRNRQQADLRYQGFVDAMQAANLPINPDWVVEGNFETEGGKAAFAKLQAVGSLP
TALFVCNDMMAIGVLHEAQRTGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAAAVHALIARLQDPNLPPQFMD
IEPRVTVRASVSQR

Sequences:

>Translated_334_residues
MATMKDVAKLASVSTSTVSHVMNATRFVSEDIAKRVQAAAQQLNYHGPSAVARSLKQNCTKTIGMLVTTSTNPFYAEVVR
SVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGLIMMCAMLEGEDALIYRRLDSLPVVVMDWGQVDFPCDKIQG
HSRQGGILATQYLIDNGHTQIGCITGPRNRQQADLRYQGFVDAMQAANLPINPDWVVEGNFETEGGKAAFAKLQAVGSLP
TALFVCNDMMAIGVLHEAQRTGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAAAVHALIARLQDPNLPPQFMD
IEPRVTVRASVSQR
>Mature_333_residues
ATMKDVAKLASVSTSTVSHVMNATRFVSEDIAKRVQAAAQQLNYHGPSAVARSLKQNCTKTIGMLVTTSTNPFYAEVVRS
VEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGLIMMCAMLEGEDALIYRRLDSLPVVVMDWGQVDFPCDKIQGH
SRQGGILATQYLIDNGHTQIGCITGPRNRQQADLRYQGFVDAMQAANLPINPDWVVEGNFETEGGKAAFAKLQAVGSLPT
ALFVCNDMMAIGVLHEAQRTGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAAAVHALIARLQDPNLPPQFMDI
EPRVTVRASVSQR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=51.8181818181818, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=42.814371257485, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=32.6860841423948, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=28.4023668639053, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=26.6467065868263, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=28.0821917808219, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786540, Length=308, Percent_Identity=29.5454545454545, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=24.8466257668712, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=23.3438485804416, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787906, Length=265, Percent_Identity=26.4150943396226, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=23.8938053097345, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=22.1153846153846, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=26.605504587156, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36578; Mature: 36446

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVAKLASVSTSTVSHVMNATRFVSEDIAKRVQAAAQQLNYHGPSAVARSLKQNCT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KTIGMLVTTSTNPFYAEVVRSVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGL
HHHHEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
IMMCAMLEGEDALIYRRLDSLPVVVMDWGQVDFPCDKIQGHSRQGGILATQYLIDNGHTQ
HHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCCCEEEEEEEEECCCEE
IGCITGPRNRQQADLRYQGFVDAMQAANLPINPDWVVEGNFETEGGKAAFAKLQAVGSLP
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCH
TALFVCNDMMAIGVLHEAQRTGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVHALIARLQDPNLPPQFMDIEPRVTVRASVSQR
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
ATMKDVAKLASVSTSTVSHVMNATRFVSEDIAKRVQAAAQQLNYHGPSAVARSLKQNCT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KTIGMLVTTSTNPFYAEVVRSVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGL
HHHHEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
IMMCAMLEGEDALIYRRLDSLPVVVMDWGQVDFPCDKIQGHSRQGGILATQYLIDNGHTQ
HHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCCCEEEEEEEEECCCEE
IGCITGPRNRQQADLRYQGFVDAMQAANLPINPDWVVEGNFETEGGKAAFAKLQAVGSLP
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCH
TALFVCNDMMAIGVLHEAQRTGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVHALIARLQDPNLPPQFMDIEPRVTVRASVSQR
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]