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The map label for this gene is folE [H]

Identifier: 167622364

GI number: 167622364

Start: 499051

End: 499710

Strand: Reverse

Name: folE [H]

Synonym: Shal_0424

Alternate gene names: 167622364

Gene position: 499710-499051 (Counterclockwise)

Preceding gene: 167622365

Following gene: 167622361

Centisome position: 9.56

GC content: 49.09

Gene sequence:

>660_bases
ATGACATCAAAAAACGCAATCCTCAGTGAATCCGCTCAGATAGTAAAATCTGCCTTAATTGAGCGCGGCCTCGAGACCCC
GATGTTGCCGAGCGAGCTAACAGCTGAAGAACGTAAAGACAAGATTGAACAGCATATGCGTGAAATCTTGACCCTAATGT
CACTGGATTTGAGCGATGATAGCCTAGCCGATACCCCACGCCGTGTCGCCAAGATGTATGTCGATGAAATTTTCTCTGGC
CTAGACTACGCCAATTTTCCCAAGATCACCGTTATCGAAAACAAGATGGGCTTCGATGAGATGGTCAAGGTTAACGGCAT
TAACCTAACCAGTACCTGTGAGCATCACTTAGTCACTATCGATGGTCTCGCTACCGTGGCCTACTTACCACGCAAGAACA
TCATCGGTCTGTCTAAGATTAACCGCATCGTGCGTTTCTTTGCCCAGCGACCACAAGTGCAGGAGCGTCTGACTCAGCAG
GTTCTAGTGGCGCTACAGGCGCTATTAGGCACTAAAGATGTGGCAGTTAAGATGGATGCGGTGCACTACTGCGTAAAATC
TCGCGGCGTGATGGACTCGACCAGCTCGACCACCACCACAGCACTGGGCGGCATCTTTAAGTCTAATCCAGCGACACGGG
CTGAATTTCTTAGTAATTAA

Upstream 100 bases:

>100_bases
ATTTTTCATTTTTTATGAGATAACTCAGTCTCTCCACGAGACTTACAGCCTAGTACGAAGGGTACGAAAATCACCACTCA
ATGTTTTGGTGGAGGAACTT

Downstream 100 bases:

>100_bases
GTTTAAAAGAAGGTTCTAGGTTTAAAAGAAGGTCCTAGGACGCTTCGCTTCGAGGACGGCCTTCGGCCTGCTAGGAAAAG
CAGAAACCAAAAACAAAAAC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTSKNAILSESAQIVKSALIERGLETPMLPSELTAEERKDKIEQHMREILTLMSLDLSDDSLADTPRRVAKMYVDEIFSG
LDYANFPKITVIENKMGFDEMVKVNGINLTSTCEHHLVTIDGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQ
VLVALQALLGTKDVAVKMDAVHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN

Sequences:

>Translated_219_residues
MTSKNAILSESAQIVKSALIERGLETPMLPSELTAEERKDKIEQHMREILTLMSLDLSDDSLADTPRRVAKMYVDEIFSG
LDYANFPKITVIENKMGFDEMVKVNGINLTSTCEHHLVTIDGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQ
VLVALQALLGTKDVAVKMDAVHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN
>Mature_218_residues
TSKNAILSESAQIVKSALIERGLETPMLPSELTAEERKDKIEQHMREILTLMSLDLSDDSLADTPRRVAKMYVDEIFSGL
DYANFPKITVIENKMGFDEMVKVNGINLTSTCEHHLVTIDGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQV
LVALQALLGTKDVAVKMDAVHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=120, Percent_Identity=46.6666666666667, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4503949, Length=120, Percent_Identity=46.6666666666667, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI66932970, Length=98, Percent_Identity=43.8775510204082, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI66932972, Length=82, Percent_Identity=48.780487804878, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1788476, Length=212, Percent_Identity=69.811320754717, Blast_Score=296, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=38.9830508474576, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=40.4371584699454, Blast_Score=125, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24656796, Length=120, Percent_Identity=47.5, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24656791, Length=120, Percent_Identity=47.5, Blast_Score=106, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24656782, Length=120, Percent_Identity=47.5, Blast_Score=106, Evalue=9e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 24290; Mature: 24159

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKNAILSESAQIVKSALIERGLETPMLPSELTAEERKDKIEQHMREILTLMSLDLSDD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLADTPRRVAKMYVDEIFSGLDYANFPKITVIENKMGFDEMVKVNGINLTSTCEHHLVTI
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCHHEEEE
DGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQALLGTKDVAVKMDA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TSKNAILSESAQIVKSALIERGLETPMLPSELTAEERKDKIEQHMREILTLMSLDLSDD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLADTPRRVAKMYVDEIFSGLDYANFPKITVIENKMGFDEMVKVNGINLTSTCEHHLVTI
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCHHEEEE
DGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQALLGTKDVAVKMDA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA